LOCUS Exported 3536 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Use to synthesize riboprobes for in situ hybridization (ISH) probe for Otx1. Not for gene expression.. ACCESSION . VERSION . KEYWORDS pISH-Otx1 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3536) AUTHORS Parrilla M, Chang I, Degl'Innocenti A, Omura M TITLE Expression of homeobox genes in the mouse olfactory epithelium. JOURNAL J Comp Neurol. 2016 Oct 1;524(14):2713-39. doi: 10.1002/cne.24051. Epub 2016 Jun 19. PUBMED 27243442 REFERENCE 2 (bases 1 to 3536) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..3536 /organism="synthetic DNA construct" /mol_type="other DNA" regulatory 33..42 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" regulatory 37..46 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" promoter complement(661..679) /label=SP6 promoter /note="promoter for bacteriophage SP6 RNA polymerase" primer_bind complement(662..679) /label=SP6 /note="SP6 promoter, forward primer" primer_bind complement(697..713) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(697..713) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(710..732) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 721..737 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(745..775) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 790..811 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(928..945) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(1099..1687) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(1179..1198) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(1858..2718) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 2481..2500 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(2719..2823) /gene="bla" /label=AmpR promoter rep_origin complement(2901..3356) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(3038..3059) /label=F1ori-F /note="F1 origin, forward primer" primer_bind 3250..3269 /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 3482..3504 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 3496..3513 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 3497..3513 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind 3520..3 /label=T7 /note="T7 promoter, forward primer" promoter 3520..2 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" ORIGIN 1 gggcgaattg ggcccgacgt cgcatgctcc cggccgccat ggcggccgcg ggaattcgat 61 tcgagagctc gggttcagag agcagcggcc agttcacgcc gcccgccgta tctagctctg 121 cttcttcgtc tagctcagcg tccagtgcct ccgccaaccc cgcggctgct gcggccgcgg 181 gcctgggtgg gaacccagtg gcagcagcgt cctctctgag cacgcctact gcttcgtcca 241 tctggagccc ggcctccatc tcgccgggct cagcgccgac atccgtatcg gtgccagagc 301 cactggccgc tccgagcaac gcctcgtgca tgcagcgctc ggtagccgca ggtgcggcca 361 ctgccgcagc ctcctaccct atgtcctatg gccagggcgg aagctatggt cagggatacc 421 ccgcgccctc ctcttcttac tttggcggtg tagactgcag ctcctaccta gcgcccatgc 481 actctcatca ccacccgcac cagcttagcc ccatggcacc ctcctccatg gctggccatc 541 atcaccatca ccctcatgcg caccacccac tgagccaatc aatcactagt gaattcgcgg 601 ccgcctgcag gtcgaccata tgggagagct cccaacgcgt tggatgcata gcttgagtat 661 tctatagtgt cacctaaata gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa 721 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg 781 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca 841 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg 901 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg 961 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg 1021 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa 1081 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg 1141 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc 1201 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc 1261 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc 1321 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg 1381 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc 1441 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga 1501 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc 1561 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac 1621 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg 1681 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc 1741 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa 1801 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta 1861 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt 1921 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag 1981 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca 2041 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc 2101 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt 2161 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag 2221 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt 2281 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat 2341 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt 2401 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc 2461 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat 2521 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag 2581 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt 2641 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg 2701 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta 2761 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc 2821 gcgcacattt ccccgaaaag tgccacctga tgcggtgtga aataccgcac agatgcgtaa 2881 ggagaaaata ccgcatcagg aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa 2941 tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa 3001 atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact 3061 attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc 3121 actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa 3181 tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc 3241 gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt 3301 cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtccat 3361 tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta 3421 cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac gccagggttt 3481 tcccagtcac gacgttgtaa aacgacggcc agtgaattgt aatacgactc actata //