LOCUS Exported 8907 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pBABEpuro-ERBB2 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 8907) AUTHORS Greulich H, Kaplan B, Mertins P, Chen TH, Tanaka KE, Yun CH, Zhang X, Lee SH, Cho J, Ambrogio L, Liao R, Imielinski M, Banerji S, Berger AH, Lawrence MS, Zhang J, Pho NH, Walker SR, Winckler W, Getz G, Frank D, Hahn WC, Eck MJ, Mani DR, Jaffe JD, Carr SA, Wong KK, Meyerson M TITLE Functional analysis of receptor tyrosine kinase mutations in lung cancer identifies oncogenic extracellular domain mutations of ERBB2. JOURNAL Proc Natl Acad Sci U S A. 2012 Sep 4;109(36):14476-81. Epub 2012 Aug 20. PUBMED 22908275 REFERENCE 2 (bases 1 to 8907) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..8907 /organism="synthetic DNA construct" /mol_type="other DNA" LTR 8..477 /note="long terminal repeat from Moloney murine leukemia virus" misc_feature 542..741 /label=MMLV Psi /note="packaging signal of Moloney murine leukemia virus (MMLV)" CDS 942..1358 /label=gag (truncated) /note="truncated Moloney murine leukemia virus (MMLV) gag gene lacking the start codon" primer_bind 1288..1310 /label=pLXSN 5' /note="Murine stem cell virus, forward primer. Also called MSCV" primer_bind 1326..1342 /label=pBABE 5' /note="Psi packaging signal, forward primer for pBABE vectors" protein_bind 1386..1410 /gene="mutant version of attB" /label=attB1 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" protein_bind complement(5176..5200) /gene="mutant version of attB" /label=attB2 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" primer_bind complement(5237..5257) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" promoter 5242..5571 /label=SV40 promoter /note="SV40 enhancer and early promoter" rep_origin 5422..5557 /label=SV40 ori /note="SV40 origin of replication" primer_bind 5484..5503 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" CDS 5581..6180 /codon_start=1 /gene="pac from Streptomyces alboniger" /product="puromycin N-acetyltransferase" /label=PuroR /note="confers resistance to puromycin" /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA" primer_bind complement(5581..5600) /label=Puro-R /note="Puromycin resistance gene, reverse primer. Also called puro-variant-R" primer_bind 6077..6097 /label=Puro-F /note="Puromycin resistance gene, forward primer" LTR 6401..6870 /note="long terminal repeat from Moloney murine leukemia virus" primer_bind complement(7000..7017) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(7171..7759) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(7251..7270) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(7919..8779) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 8542..8561 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(8780..8884) /gene="bla" /label=AmpR promoter ORIGIN 1 ctgcagcctg aatatgggcc aaacaggata tctgtggtaa gcagttcctg ccccggctca 61 gggccaagaa cagatggaac agctgaatat gggccaaaca ggatatctgt ggtaagcagt 121 tcctgccccg gctcagggcc aagaacagat ggtccccaga tgcggtccag ccctcagcag 181 tttctagaga accatcagat gtttccaggg tgccccaagg acctgaaatg accctgtgcc 241 ttatttgaac taaccaatca gttcgcttct cgcttctgtt cgcgcgcttc tgctccccga 301 gctcaataaa agagcccaca acccctcact cggggcgcca gtcctccgat tgactgagtc 361 gcccgggtac ccgtgtatcc aataaaccct cttgcagttg catccgactt gtggtctcgc 421 tgttccttgg gagggtctcc tctgagtgat tgactacccg tcagcggggg tctttcattt 481 gggggctcgt ccgggatcgg gagacccctg cccagggacc accgacccac caccgggagg 541 caagctggcc agcaacttat ctgtgtctgt ccgattgtct agtgtctatg actgatttta 601 tgcgcctgcg tcggtactag ttagctaact agctctgtat ctggcggacc cgtggtggaa 661 ctgacgagtt ctgaacaccc ggccgcaacc ctgggagacg tcccagggac tttgggggcc 721 gtttttgtgg cccgacctga ggaagggagt cgatgtggaa tccgaccccg tcaggatatg 781 tggttctggt aggagacgag aacctaaaac agttcccgcc tccgtctgaa tttttgcttt 841 cggtttggaa ccgaagccgc gcgtcttgtc tgctgcagcg ctgcagcatc gttctgtgtt 901 gtctctgtct gactgtgttt ctgtatttgt ctgaaaatta gggccagact gttaccactc 961 ccttaagttt gaccttaggt cactggaaag atgtcgagcg gatcgctcac aaccagtcgg 1021 tagatgtcaa gaagagacgt tgggttacct tctgctctgc agaatggcca acctttaacg 1081 tcggatggcc gcgagacggc acctttaacc gagacctcat cacccaggtt aagatcaagg 1141 tcttttcacc tggcccgcat ggacacccag accaggtccc ctacatcgtg acctgggaag 1201 ccttggcttt tgacccccct ccctgggtca agccctttgt acaccctaag cctccgcctc 1261 ctcttcctcc atccgccccg tctctccccc ttgaacctcc tcgttcgacc ccgcctcgat 1321 cctcccttta tccagccctc actccttctc taggcgccgg ccggatccca gtgtggtggt 1381 acatcacaag tttgtacaaa aaagttggca tggagctggc ggccttgtgc cgctgggggc 1441 tcctcctcgc cctcttgccc cccggagccg cgagcaccca agtgtgcacc ggcacagaca 1501 tgaagctgcg gctccctgcc agtcccgaga cccacctgga catgctccgc cacctctacc 1561 agggctgcca ggtggtgcag ggaaacctgg aactcaccta cctgcccacc aatgccagcc 1621 tgtccttcct gcaggatatc caggaggtgc agggctacgt gctcatcgct cacaaccaag 1681 tgaggcaggt cccactgcag aggctgcgga ttgtgcgagg cacccagctc tttgaggaca 1741 actatgccct ggccgtgcta gacaatggag acccgctgaa caataccacc cctgtcacag 1801 gggcctcccc aggaggcctg cgggagctgc agcttcgaag cctcacagag atcttgaaag 1861 gaggggtctt gatccagcgg aacccccagc tctgctacca ggacacgatt ttgtggaagg 1921 acatcttcca caagaacaac cagctggctc tcacactgat agacaccaac cgctctcggg 1981 cctgccaccc ctgttctccg atgtgtaagg gctcccgctg ctggggagag agttctgagg 2041 attgtcagag cctgacgcgc actgtctgtg ccggtggctg tgcccgctgc aaggggccac 2101 tgcccactga ctgctgccat gagcagtgtg ctgccggctg cacgggcccc aagcactctg 2161 actgcctggc ctgcctccac ttcaaccaca gtggcatctg tgagctgcac tgcccagccc 2221 tggtcaccta caacacagac acgtttgagt ccatgcccaa tcccgagggc cggtatacat 2281 tcggcgccag ctgtgtgact gcctgtccct acaactacct ttctacggac gtgggatcct 2341 gcaccctcgt ctgccccctg cacaaccaag aggtgacagc agaggatgga acacagcggt 2401 gtgagaagtg cagcaagccc tgtgcccgag tgtgctatgg tctgggcatg gagcacttgc 2461 gagaggtgag ggcagttacc agtgccaata tccaggagtt tgctggctgc aagaagatct 2521 ttgggagcct ggcatttctg ccggagagct ttgatgggga cccagcctcc aacactgccc 2581 cgctccagcc agagcagctc caagtgtttg agactctgga agagatcaca ggttacctat 2641 acatctcagc atggccggac agcctgcctg acctcagcgt cttccagaac ctgcaagtaa 2701 tccggggacg aattctgcac aatggcgcct actcgctgac cctgcaaggg ctgggcatca 2761 gctggctggg gctgcgctca ctgagggaac tgggcagtgg actggccctc atccaccata 2821 acacccacct ctgcttcgtg cacacggtgc cctgggacca gctctttcgg aacccgcacc 2881 aagctctgct ccacactgcc aaccggccag aggacgagtg tgtgggcgag ggcctggcct 2941 gccaccagct gtgcgcccga gggcactgct ggggtccagg gcccacccag tgtgtcaact 3001 gcagccagtt ccttcggggc caggagtgcg tggaggaatg ccgagtactg caggggctcc 3061 ccagggagta tgtgaatgcc aggcactgtt tgccgtgcca ccctgagtgt cagccccaga 3121 atggctcagt gacctgtttt ggaccggagg ctgaccagtg tgtggcctgt gcccactata 3181 aggaccctcc cttctgcgtg gcccgctgcc ccagcggtgt gaaacctgac ctctcctaca 3241 tgcccatctg gaagtttcca gatgaggagg gcgcatgcca gccttgcccc atcaactgca 3301 cccactcctg tgtggacctg gatgacaagg gctgccccgc cgagcagaga gccagccctc 3361 tgacgtccat catctctgcg gtggttggca ttctgctggt cgtggtcttg ggggtggtct 3421 ttgggatcct catcaagcga cggcagcaga agatccggaa gtacacgatg cggagactgc 3481 tgcaggaaac ggagctggtg gagccgctga cacctagcgg agcgatgccc aaccaggcgc 3541 agatgcggat cctgaaagag acggagctga ggaaggtgaa ggtgcttgga tctggcgctt 3601 ttggcacagt ctacaagggc atctggatcc ctgatgggga gaatgtgaaa attccagtgg 3661 ccatcaaagt gttgagggaa aacacatccc ccaaagccaa caaagaaatc ttagacgaag 3721 catacgtgat ggctggtgtg ggctccccat atgtctcccg ccttctgggc atctgcctga 3781 catccacggt gcagctggtg acacagctta tgccctatgg ctgcctctta gaccatgtcc 3841 gggaaaaccg cggacgcctg ggctcccagg acctgctgaa ctggtgtatg cagattgcca 3901 aggggatgag ctacctggag gatgtgcggc tcgtacacag ggacttggcc gctcggaacg 3961 tgctggtcaa gagtcccaac catgtcaaaa ttacagactt cgggctggct cggctgctgg 4021 acattgacga gacagagtac catgcagatg ggggcaaggt gcccatcaag tggatggcgc 4081 tggagtccat tctccgccgg cggttcaccc accagagtga tgtgtggagt tatggtgtga 4141 ctgtgtggga gctgatgact tttggggcca aaccttacga tgggatccca gcccgggaga 4201 tccctgacct gctggaaaag ggggagcggc tgccccagcc ccccatctgc accattgatg 4261 tctacatgat catggtcaaa tgttggatga ttgactctga atgtcggcca agattccggg 4321 agttggtgtc tgaattctcc cgcatggcca gggaccccca gcgctttgtg gtcatccaga 4381 atgaggactt gggcccagcc agtcccttgg acagcacctt ctaccgctca ctgctggagg 4441 acgatgacat gggggacctg gtggatgctg aggagtatct ggtaccccag cagggcttct 4501 tctgtccaga ccctgccccg ggcgctgggg gcatggtcca ccacaggcac cgcagctcat 4561 ctaccaggag tggcggtggg gacctgacac tagggctgga gccctctgaa gaggaggccc 4621 ccaggtctcc actggcaccc tccgaagggg ctggctccga tgtatttgat ggtgacctgg 4681 gaatgggggc agccaagggg ctgcaaagcc tccccacaca tgaccccagc cctctacagc 4741 ggtacagtga ggaccccaca gtacccctgc cctctgagac tgatggctac gttgcccccc 4801 tgacctgcag cccccagcct gaatatgtga accagccaga tgttcggccc cagccccctt 4861 cgccccgaga gggccctctg cctgctgccc gacctgctgg tgccactctg gaaaggccca 4921 agactctctc cccagggaag aatggggtcg tcaaagacgt ttttgccttt gggggtgccg 4981 tggagaaccc cgagtacttg acaccccagg gaggagctgc ccctcagccc caccctcctc 5041 ctgccttcag cccagccttc gacaacctct attactggga ccaggaccca ccagagcggg 5101 gggctccacc cagcaccttc aaagggacac ctacggcaga gaacccagag tacctgggtc 5161 tggacgtgcc agtgtgacca actttcttgt acaaagtggt gatgtaggaa ttcgccagca 5221 cagtggtcga ccctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca 5281 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc 5341 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata 5401 gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg 5461 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag 5521 ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagcttacc 5581 atgaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc cagggccgta 5641 cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt cgatccggac 5701 cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac 5761 atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac cacgccggag 5821 agcgtcgaag cgggggcggt gttcgccgag atcggcccgc gcatggccga gttgagcggt 5881 tcccggctgg ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg gcccaaggag 5941 cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc 6001 agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg 6061 gagacctccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac cgtcaccgcc 6121 gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc cggtgcctga 6181 cgcccgcccc acgacccgca gcgcccgacc gaaaggagcg cacgacccca tgcatcgata 6241 aaataaaaga ttttatttag tctccagaaa aaggggggaa tgaaagaccc cacctgtagg 6301 tttggcaagc tagcttaagt aacgccattt tgcaaggcat ggaaaaatac ataactgaga 6361 atagagaagt tcagatcaag gtcaggaaca gatggaacag ctgaatatgg gccaaacagg 6421 atatctgtgg taagcagttc ctgccccggc tcagggccaa gaacagatgg aacagctgaa 6481 tatgggccaa acaggatatc tgtggtaagc agttcctgcc ccggctcagg gccaagaaca 6541 gatggtcccc agatgcggtc cagccctcag cagtttctag agaaccatca gatgtttcca 6601 gggtgcccca aggacctgaa atgaccctgt gccttatttg aactaaccaa tcagttcgct 6661 tctcgcttct gttcgcgcgc ttctgctccc cgagctcaat aaaagagccc acaacccctc 6721 actcggggcg ccagtcctcc gattgactga gtcgcccggg tacccgtgta tccaataaac 6781 cctcttgcag ttgcatccga cttgtggtct cgctgttcct tgggagggtc tcctctgagt 6841 gattgactac ccgtcagcgg gggtctttca catgcagcat gtatcaaaat taatttggtt 6901 ttttttctta agtatttaca ttaaatggcc atagttgcat taatgaatcg gccaacgcgc 6961 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg 7021 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc 7081 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag 7141 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca 7201 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca 7261 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg 7321 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag 7381 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt 7441 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca 7501 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg 7561 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt 7621 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc 7681 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg 7741 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg 7801 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta 7861 gatccttttg cggccgcaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt 7921 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag 7981 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca 8041 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc 8101 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt 8161 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg 8221 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca 8281 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg 8341 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca 8401 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg 8461 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct 8521 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca 8581 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca 8641 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg 8701 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac 8761 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt 8821 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc 8881 cgcgcacatt tccccgaaaa gtgccac //