LOCUS       Exported                 885 bp ds-DNA     linear   SYN 30-MAY-2021
DEFINITION  Expresses GA-inducible VPR-Sp dCas9.
ACCESSION   .
VERSION     .
KEYWORDS    pSLQ2812 pPB: CAG-GID1-VPR-IRES-Puro-WPRE PGK-GAI-tagBFP-SpdCas9
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 885)
  AUTHORS   Gao Y, Xiong X, Wong S, Charles EJ, Lim WA, Qi LS
  TITLE     Complex transcriptional modulation with orthogonal and inducible 
            dCas9 regulators.
  JOURNAL   Nat Methods. 2016 Oct 24. doi: 10.1038/nmeth.4042.
  PUBMED    27776111
REFERENCE   2  (bases 1 to 885)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 30, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..885
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             complement(283..300)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     promoter        complement(342..841)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(390..409)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     783..806
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
ORIGIN
        1 ctcgctcatg gtagcacctg cagcgcttga catatgatta aggtcggtga gcatagaatc
       61 aagccacgtg taaagctccg ccggattata gtgaacagtc tcagtagcga gttgagaaag
      121 atcgtcttct tgaacattag acatcataac ttcaagctgc tcgagtttct gagcaacatc
      181 agccatttcg gatgacctaa ccttgtaacc aagaacagct agaagctcat ccatgccgtt
      241 accgtcgtct tcttcattca tcatcatagt cttcttatct tgatgatgat gatgatgatg
      301 atctctcttc atggtggcgt cgacaagctt gggctgcagg tcgaaaggcc cggagatgag
      361 gaagaggaga acagcgcggc agacgtgcgc ttttgaagcg tgcagaatgc cgggcctccg
      421 gaggaccttc gggcgcccgc cccgcccctg agcccgcccc tgagcccgcc cccggaccca
      481 ccccttccca gcctctgagc ccagaaagcg aaggagcaaa gctgctattg gccgctgccc
      541 caaaggccta cccgcttcca ttgctcagcg gtgctgtcca tctgcacgag cctagtgaga
      601 cgtgctactt ccatttgtca cgtcctgcac gacgcgagct gcggggcggg ggggaacttc
      661 ctgactaggg gaggagtaga aggtggcgcg aaggggccac caaagaacgg agccggttgg
      721 cgcctaccgg tggatgtgga atgtgtgcga ggccagaggc cacttgtgta gcgccaagtg
      781 cccagcgggg ctgctaaagc gcatgctcca gactgccttg ggaaaagcgc ctcccctacc
      841 cggtagaatt acctgcagga tgcggggagg cggcccaaag ggaga
//
