LOCUS       Exported               12398 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  lentiviral expression of human VE-cadherin, C-terminally tagged with
            mRuby3.
ACCESSION   .
VERSION     .
KEYWORDS    pLV-CDH5-mRuby3-IRES-puro
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12398)
  AUTHORS   Hayer A, Shao L, Chung M, Joubert LM, Yang HW, Tsai FC, Bisaria A, 
            Betzig E, Meyer T
  TITLE     Engulfed cadherin fingers are polarized junctional structures 
            between collectively migrating endothelial cells.
  JOURNAL   Nat Cell Biol. 2016 Dec;18(12):1311-1323. doi: 10.1038/ncb3438. Epub
            2016 Nov 14.
  PUBMED    27842057
REFERENCE   2  (bases 1 to 12398)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..12398
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2028..2143
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     enhancer        2206..2585
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2588..2863
                     /label=chicken beta-actin promoter
     intron          2864..3872
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     3796..3818
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     3880..3899
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             complement(5974..5985)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(6299..6323)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             6331..7041
                     /codon_start=1
                     /product="monomeric red fluorescent protein derived from 
                     mRuby2, with improved brightness and photostability (Bajar 
                     et al., 2016)"
                     /label=mRuby3
                     /translation="MVSKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGRPYEGVQTM
                     RIKVIEGGPLPFAFDILATSFMYGSRTFIKYPADIPDFFKQSFPEGFTWERVTRYEDGG
                     VVTVTQDTSLEDGELVYNVKVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTD
                     IALKVDGGGHLHCNFVTTYRSKKTVGNIKMPGVHAVDHRLERIEESDNETYVVQREVAV
                     AKYSNLGGGMDELYK"
     primer_bind     complement(7289..7306)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     7516..7535
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             7697..8296
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(7697..7716)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8193..8213
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    8320..8908
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8373..8393)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8791..8802)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             9116..9749
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(9878..9894)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9878..9894)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9891..9913)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9902..9918
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(9926..9956)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9971..9992
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10109..10126)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10280..10865)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10360..10379)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(11036..11896)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11659..11678
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11897..12001)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    12049..12183
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(12086..12105)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     12140..12159
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga actacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgatgaggcc ctttcgtctt cactcgacat tgattattga
     2221 ctagttatta atagtaatca attacggggt cattagttca tagcccatat atggagttcc
     2281 gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat
     2341 tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc
     2401 aatgggtgga ctatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc
     2461 caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt
     2521 acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta
     2581 ccatgggtcg aggtgagccc cacgttctgc ttcactctcc ccatctcccc cccctcccca
     2641 cccccaattt tgtatttatt tattttttaa ttattttgtg cagcgatggg ggcggggggg
     2701 gggggggcgc gcgccaggcg gggcggggcg gggcgagggg cggggcgggg cgaggcggag
     2761 aggtgcggcg gcagccaatc agagcggcgc gctccgaaag tttcctttta tggcgaggcg
     2821 gcggcggcgg cggccctata aaaagcgaag cgcgcggcgg gcgggagtcg ctgcgttgcc
     2881 ttcgccccgt gccccgctcc gcgccgcctc gcgccgcccg ccccggctct gactgaccgc
     2941 gttactccca caggtgagcg ggcgggacgg cccttctcct ccgggctgta attagcgctt
     3001 ggtttaatga cggctcgttt cttttctgtg gctgcgtgaa agccttaaag ggctccggga
     3061 gggccctttg tgcggggggg agcggctcgg ggggtgcgtg cgtgtgtgtg tgcgtgggga
     3121 gcgccgcgtg cggcccgcgc tgcccggcgg ctgtgagcgc tgcgggcgcg gcgcggggct
     3181 ttgtgcgctc cgcgtgtgcg cgaggggagc gcggccgggg gcggtgcccc gcggtgcggg
     3241 ggggctgcga ggggaacaaa ggctgcgtgc ggggtgtgtg cgtggggggg tgagcagggg
     3301 gtgtgggcgc ggcggtcggg ctgtaacccc cccctgcacc cccctccccg agttgctgag
     3361 cacggcccgg cttcgggtgc ggggctccgt gcggggcgtg gcgcggggct cgccgtgccg
     3421 ggcggggggt ggcggcaggt gggggtgccg ggcggggcgg ggccgcctcg ggccggggag
     3481 ggctcggggg aggggcgcgg cggccccgga gcgccggcgg ctgtcgaggc gcggcgagcc
     3541 gcagccattg ccttttatgg taatcgtgcg agagggcgca gggacttcct ttgtcccaaa
     3601 tctggcggag ccgaaatctg ggaggcgccg ccgcaccccc tctagcgggc gcgggcgaag
     3661 cggtgcggcg ccggcaggaa ggaaatgggc ggggagggcc ttcgtgcgtc gccgcgccgc
     3721 cgtccccttc tccatctcca gcctcggggc tgccgcaggg ggacggctgc cttcgggggg
     3781 gacggggcag ggcggggttc ggcttctggc gtgtgaccgg cggctctaga gcctctgcta
     3841 accatgttca tgccttcttc tttttcctac agctcctggg caacgtgctg gttattgtgc
     3901 tgtctcatca ttttggcaaa gaattccacc atgcagaggc tcatgatgct cctcgccaca
     3961 tcgggcgcct gcctgggcct gctggcagtg gcagcagtgg cagcagcagg tgctaaccct
     4021 gcccaacggg acacccacag cctgctgccc acccaccggc gccaaaagag agattggatt
     4081 tggaaccaga tgcacattga tgaagagaaa aacacctcac ttccccatca tgtaggcaag
     4141 atcaagtcaa gcgtgagtcg caagaatgcc aagtacctgc tcaaaggaga atatgtgggc
     4201 aaggtcttcc gggtcgatgc agagacagga gacgtgttcg ccattgagag gctggaccgg
     4261 gagaatatct cagagtacca cctcactgct gtcattgtgg acaaggacac tggcgaaaac
     4321 ctggagactc cttccagctt caccatcaaa gttcatgacg tgaacgacaa ctggcctgtg
     4381 ttcacgcatc ggttgttcaa tgcgtccgtg cctgagtcgt cggctgtggg gacctcagtc
     4441 atctctgtga cagcagtgga tgcagacgac cccactgtgg gagaccacgc ctctgtcatg
     4501 taccaaatcc tgaaggggaa agagtatttt gccatcgata attctggacg tattatcaca
     4561 ataacgaaaa gcttggaccg agagaagcag gccaggtatg agatcgtggt ggaagcgcga
     4621 gatgcccagg gcctccgggg ggactcgggc acggccaccg tgctggtcac tctgcaagac
     4681 atcaatgaca acttcccctt cttcacccag accaagtaca catttgtcgt gcctgaagac
     4741 acccgtgtgg gcacctctgt gggctctctg tttgttgagg acccagatga gccccagaac
     4801 cggatgacca agtacagcat cttgcggggc gactaccagg acgctttcac cattgagaca
     4861 aaccccgccc acaacgaggg catcatcaag cccatgaagc ctctggatta tgaatacatc
     4921 cagcaataca gcttcatcgt cgaggccaca gaccccacca tcgacctccg atacatgagc
     4981 cctcccgcgg gaaacagagc ccaggtcatt atcaacatca cagatgtgga cgagcccccc
     5041 attttccagc agcctttcta ccacttccag ctgaaggaaa accagaagaa gcctctgatt
     5101 ggcacagtgc tggccatgga ccctgatgcg gctaggcata gcattggata ctccatccgc
     5161 aggaccagtg acaagggcca gttcttccga gtcacaaaaa agggggacat ttacaatgag
     5221 aaagaactgg acagagaagt ctacccctgg tataacctga ctgtggaggc caaagaactg
     5281 gattccactg gaacccccac aggaaaagaa tccattgtgc aagtccacat tgaagttttg
     5341 gatgagaatg acaatgcccc ggagtttgcc aagccctacc agcccaaagt gtgtgagaac
     5401 gctgtccatg gccagctggt cctgcagatc tccgcaatag acaaggacat aacaccacga
     5461 aacgtgaagt tcaaattcac cttgaatact gagaacaact ttaccctcac ggataatcac
     5521 gataacacgg ccaacatcac agtcaagtat gggcagtttg accgggagca taccaaggtc
     5581 cacttcctac ccgtggtcat ctcagacaat gggatgccaa gtcgcacggg caccagcacg
     5641 ctgaccgtgg ccgtgtgcaa gtgcaacgag cagggcgagt tcaccttctg cgaggatatg
     5701 gccgcccagg tgggcgtgag catccaggca gtggtagcca tcttactctg catcctcacc
     5761 atcacagtga tcaccctgct catcttcctg cggcggcggc tccggaagca ggcccgcgcg
     5821 cacggcaaga gcgtgccgga gatccacgag cagctggtca cctacgacga ggagggcggc
     5881 ggcgagatgg acaccaccag ctacgatgtg tcggtgctca actcggtgcg ccgcggcggg
     5941 gccaagcccc cgcggcccgc gctggacgcc cggccttccc tctatgcgca ggtgcagaag
     6001 ccaccgaggc acgcgcctgg ggcacacgga gggcccgggg agatggcagc catgatcgag
     6061 gtgaagaagg acgaggcgga ccacgacggc gacggccccc cctacgacac gctgcacatc
     6121 tacggctacg agggctccga gtccatagcc gagtccctca gctccctggg caccgactca
     6181 tccgactctg acgtggatta cgacttcctt aacgactggg gacccaggtt taagatgctg
     6241 gctgagctgt acggctcgga cccccgggag gagctgctgt ataagggtgg gcgcgccgac
     6301 ccagctttct tgtacaaagt ggtaccggtc atggtgtcta agggcgaaga gctgatcaag
     6361 gaaaatatgc gtatgaaggt ggtcatggaa ggttcggtca acggccacca attcaaatgc
     6421 acaggtgaag gagaaggcag accgtacgag ggagtgcaaa ccatgaggat caaagtcatc
     6481 gagggaggac ccctgccatt tgcctttgac attcttgcca cgtcgttcat gtatggcagc
     6541 cgtaccttta tcaagtaccc ggccgacatc cctgatttct ttaaacagtc ctttcctgag
     6601 ggttttactt gggaaagagt tacgagatac gaagatggtg gagtcgtcac cgtcacgcag
     6661 gacaccagcc ttgaggatgg cgagctcgtc tacaacgtca aggtcagagg ggtaaacttt
     6721 ccctccaatg gtcccgtgat gcagaagaag accaagggtt gggagcctaa tacagagatg
     6781 atgtatccag cagatggtgg tctgagagga tacactgaca tcgcactgaa agttgatggt
     6841 ggtggccatc tgcactgcaa cttcgtgaca acttacaggt caaaaaagac cgtcgggaac
     6901 atcaagatgc ccggtgtcca tgccgttgat caccgcctgg aaaggatcga ggagagtgac
     6961 aatgaaacct acgtagtgca aagagaagtg gcagttgcca aatacagcaa ccttggtggt
     7021 ggcatggacg agctgtacaa gtaagcggcc gctctagagt cgacgggccg cggtaacaat
     7081 tgttaactaa cttaagctag caacggtttc cctctagcgg gatcaattcc gccccccccc
     7141 cctaacgtta ctggccgaag ccgcttggaa taaggccggt gtgcgtttgt ctatatgtta
     7201 ttttccacca tattgccgtc ttttggcaat gtgagggccc ggaaacctgg ccctgtcttc
     7261 ttgacgagca ttcctagggg tctttcccct ctcgccaaag gaatgcaagg tctgttgaat
     7321 gtcgtgaagg aagcagttcc tctggaagct tcttgaagac aaacaacgtc tgtagcgacc
     7381 ctttgcaggc agcggaaccc cccacctggc gacaggtgcc tctgcggcca aaagccacgt
     7441 gtataagata cacctgcaaa ggcggcacaa ccccagtgcc acgttgtgag ttggatagtt
     7501 gtggaaagag tcaaatggct ctcctcaagc gtattcaaca aggggctgaa ggatgcccag
     7561 aaggtacccc attgtatggg atctgatctg gggcctcggt gcacatgctt tacatgtgtt
     7621 tagtcgaggt taaaaaaacg tctaggcccc ccgaaccacg gggacgtggt tttcctttga
     7681 aaaacacgat aataccatga ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga
     7741 cgtccccagg gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca
     7801 caccgtcgat ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac
     7861 gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt
     7921 ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat
     7981 ggccgagttg agcggttccc ggctggccgc gcagcaacag atggaaggcc tcctggcgcc
     8041 gcaccggccc aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca
     8101 gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg
     8161 ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc cccttctacg agcggctcgg
     8221 cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg
     8281 caagcccggt gcctgacccg ggcggggcgc gtctggaaca atcaacctct ggattacaaa
     8341 atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac
     8401 gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc
     8461 ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt
     8521 ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc
     8581 tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc
     8641 gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg
     8701 gtgttgtcgg ggaagctgac gtcctttcca tggctgctcg cctgtgttgc cacctggatt
     8761 ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc
     8821 cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt
     8881 cggatctccc tttgggccgc ctccccgcct ggaattaatt ctgcagtcga gacctagaaa
     8941 aacatggagc aatcacaagt agcaatacag cagctaccaa tgctgattgt gcctggctag
     9001 aagcacaaga ggaggaggag gtgggttttt ccagtcacac ctcaggtacc tttaagacca
     9061 atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaagag gggactggaa
     9121 gggctaattc actcccaacg aagacaagat atccttgatc tgtggatcta ccacacacaa
     9181 ggctacttcc ctgattagca gaactacaca ccagggccag gggtcagata tccactgacc
     9241 tttggatggt gctacaagct agtaccagtt gagccagata aggtagaaga ggccaataaa
     9301 ggagagaaca ccagcttgtt acaccctgtg agcctgcatg ggatggatga cccggagaga
     9361 gaagtgttag agtggaggtt tgacagccgc ctagcatttc atcacgtggc ccgagagctg
     9421 catccggagt acttcaagaa ctgctgatat cgagcttgct acaagggact ttccgctggg
     9481 gactttccag ggaggcgtgg cctgggcggg actggggagt ggcgagccct cagatcctgc
     9541 atataagcag ctgctttttg cctgtactgg gtctctctgg ttagaccaga tctgagcctg
     9601 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt
     9661 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc
     9721 cttttagtca gtgtggaaaa tctctagcag tagtagttca tgtcatctta ttattcagta
     9781 tttataactt gcaaagaaat gaatatcaga gagtgagagg ccttgacatt gctagcgttt
     9841 taccgtcgac ctctagctag agcttggcgt aatcatggtc atagctgttt cctgtgtgaa
     9901 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
     9961 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc
    10021 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
    10081 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
    10141 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
    10201 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
    10261 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
    10321 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
    10381 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
    10441 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
    10501 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
    10561 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
    10621 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
    10681 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
    10741 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
    10801 ccaccgctgg tagcggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
    10861 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
    10921 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
    10981 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
    11041 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
    11101 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
    11161 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
    11221 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
    11281 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
    11341 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
    11401 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
    11461 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
    11521 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
    11581 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
    11641 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
    11701 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
    11761 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
    11821 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
    11881 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
    11941 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
    12001 gcacatttcc ccgaaaagtg ccacctgacg tcgacggatc gggagatcaa cttgtttatt
    12061 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
    12121 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg
    12181 atcaactgga taactcaagc taaccaaaat catcccaaac ttcccacccc ataccctatt
    12241 accactgcca attacctagt ggtttcattt actctaaacc tgtgattcct ctgaattatt
    12301 ttcattttaa agaaattgta tttgttaaat atgtactaca aacttagtag tttttaaaga
    12361 aattgtattt gttaaatatg tactacaaac ttagtagt
//
