LOCUS Exported 1009 bp ds-DNA linear SYN 21-MAY-2021 DEFINITION rAAV-based template for genome engineering of the MDM2 protein C-terminus containing PQS2 and 3xHA tags and a selection cassette. ACCESSION . VERSION . KEYWORDS pAav-MDM2-PQS2-3xHA SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 1009) AUTHORS Vandemoortele G, Staes A, Gonnelli G, Samyn N, De Sutter D, Vandermarliere E, Timmerman E, Gevaert K, Martens L, Eyckerman S TITLE An extra dimension in protein tagging by quantifying universal proteotypic peptides using targeted proteomics. JOURNAL Sci Rep. 2016 Jun 6;6:27220. doi: 10.1038/srep27220. PUBMED 27264994 REFERENCE 2 (bases 1 to 1009) AUTHORS . TITLE Direct Submission JOURNAL Exported May 21, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..1009 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind complement(121..138) /label=BGH-rev /note="Bovine growth hormone terminator, reverse primer. Also called BGH reverse" polyA_signal 127..351 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" protein_bind 376..409 /label=loxP /bound_moiety="Cre recombinase" /note="Cre-mediated recombination occurs in the 8-bp core sequence (GCATACAT)." ORIGIN 1 gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct 61 tctatcgcct tcttgacgag ttcttctgag gggatcaatt ctctagagct cgctgatcag 121 cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct 181 tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc 241 attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg 301 aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg gcttctgagg 361 cggaaagaac cagctataac ttcgtatagc atacattata cgaagttata gtaatcgata 421 actcgagtag ttgacctgtc tataagagaa ttatatattt ctaactatat aaccctagga 481 atttagacaa cctgaaattt attcacatat atcaaagtga gaaaatgcct caattcacat 541 agatttcttc tctttagtat aattgaccta ctttggtagt ggaatagtga atacttacta 601 taatttgact tgaatatgta gctcatcctt tacaccaact cctaatttta aataatttct 661 actctgtctt aaatgagaag tacttggttt tttttttctt aaatatgtat atgacattta 721 aatgtaactt attatttttt ttgagaccga gtcttgctct gttacccagg ctggagtgca 781 gtggcgtgat cttggctcac tgcaagctct gcctcccggg ttcgcaccat tctcctgcct 841 cagcctccca attagcttgg cctacagtca tctgccacca cacctggcta attttttgta 901 cttttagtag agacagggtt tcaccgtgtt agccaggatg gtctcgatct cctgacctcg 961 tgatccgccc acctcggcct cccaaagtgc tgggattaca ggcatgagc //