LOCUS       Exported                 873 bp ds-DNA     linear   SYN 06-JUN-2021
DEFINITION  MESA protease chain with V1-MESA ectodomain, 45 extracellular 
            linkers, a flag tag, and Tev protease.
ACCESSION   .
VERSION     .
KEYWORDS    V1-MESA-45F-Tev
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 873)
  AUTHORS   Schwarz KA, Daringer NM, Dolberg TB, Leonard JN
  TITLE     Rewiring human cellular input-output using modular extracellular 
            sensors.
  JOURNAL   Nat Chem Biol. 2016 Dec 12. doi: 10.1038/nchembio.2253.
  PUBMED    27941759
REFERENCE   2  (bases 1 to 873)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 6, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..873
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             complement(164..868)
                     /codon_start=1
                     /product="tobacco etch virus protease (Kapust et al., 
                     2001)"
                     /label=TEV protease
                     /translation="ESLFKGPRDYNPISSTICHLTNESDGHTTSLYGIGFGPFIITNKH
                     LFRRNNGTLLVQSLHGVFKVKNTTTLQQHLIDGRDMIIIRMPKDFPPFPQKLKFREPQR
                     EERICLVTTNFQTKSMSSMVSDTSCTFPSSDGIFWKHWIQTKDGQCGSPLVSTRDGFIV
                     GIHSASNFTNTNNYFTSVPKNFMELLTNQEAQQWVSGWRLNADSVLWGGHKVFMVKPEE
                     PFQPVKEATQLMN"
ORIGIN
        1 aaggaacctt taatagaaat tggacagcaa gaaagcgagc ttagtgatac ttgtgggcca
       61 gggcattagc cacaccagcc accactttct gataggcagc ctgcactggt ggggtgaatt
      121 gctcgaggtt taaacagctc caccgcggtg gcggccgcta ctaattcatg agttgagtcg
      181 cttccttaac tggctgaaaa ggctcttcag gtttcaccat gaaaacttta tggccccccc
      241 acaatactga gtcagcattt aatcgccaac cactaaccca ctgctgcgcc tcctgatttg
      301 tcaacaattc catgaagttt ttcggcacgc ttgtgaaata attgtttgtg ttggtgaaat
      361 tcgatgctga gtgtatacca acaatgaacc catctctagt tgatactaat ggactgccac
      421 actgcccatc cttggtttga atccaatgct tccagaatat gccatcagat gaagggaatg
      481 tgcaactagt gtctgacacc atgctagaca tgctcttagt ttggaagttg gttgtcacaa
      541 gacatatgcg ctcttccctt tgtggctctc taaatttcag cttttgagga aatggtggga
      601 aatccttagg catgcgaata attatcatgt ccctcccatc aatgaggtgt tgttgcaaag
      661 tcgtggtgtt cttgaccttg aatacaccat gtagtgattg gaccaacagt gttccattat
      721 ttcttctaaa caagtgcttg tttgtaatga tgaagggacc aaatccaata ccatacaacg
      781 atgttgtgtg cccatcagat tcattcgtca aatgacaaat ggtgctcgat atcgggttgt
      841 aatcacgcgg ccccttaaac aagctctcgt ttt
//
