LOCUS       Exported                4655 bp ds-DNA     circular SYN 13-MAY-2021
DEFINITION  Ebola Virus (Makona) Glycoprotein in CMV driven mammalian expression
            vector.
ACCESSION   .
VERSION     .
KEYWORDS    pGL4.23 A82V/T230A/D637G 2014 EBOV Delta-Mucin-Like-Domain
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4655)
  AUTHORS   Diehl WE, Lin AE, Grubaugh ND, Carvalho LM, Kim K, Kyawe PP, 
            McCauley SM, Donnard E, Kucukural A, McDonel P, Schaffner SF, Garber
            M, Rambaut A, Andersen KG, Sabeti PC, Luban J
  TITLE     Ebola Virus Glycoprotein with Increased Infectivity Dominated the 
            2013-2016 Epidemic.
  JOURNAL   Cell. 2016 Nov 3;167(4):1088-1098.e6. doi: 
            10.1016/j.cell.2016.10.014.
  PUBMED    27814506
REFERENCE   2  (bases 1 to 4655)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 13, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4655
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        30..409
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     564..584
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     promoter        618..649
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     regulatory      676..685
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     polyA_signal    2209..2330
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2220..2239)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2274..2293
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(2578..2595)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2749..3337)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2829..2848)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(3537..4397)
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     polyA_signal    4502..4550
                     /note="synthetic polyadenylation signal"
     misc_feature    4564..4655
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     4604..4623
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
ORIGIN
        1 ggcctaactg gccgccagat atacgcgttg acattgatta ttgactagtt attaatagta
       61 atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac
      121 ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac
      181 gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt
      241 acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat
      301 tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga
      361 ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt
      421 ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca
      481 ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg
      541 tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt ggggcctcgg
      601 cggccaagct tagacactag agggtatata atggaagctc gacttccagc ttggcaatcc
      661 ggtactgttg gtaaagccac catgggtgtt acaggaatat tgcagttacc tcgtgatcga
      721 ttcaagagga catcattctt tctttgggta attatccttt tccaaagaac attttccatc
      781 ccgcttggag ttatccacaa tagtacatta caggttagtg atgtcgacaa actagtttgt
      841 cgtgacaaac tgtcatccac aaatcaattg agatcagttg gactgaatct cgaggggaat
      901 ggagtggcaa ctgacgtgcc atctgtgact aaaagatggg gcttcaggtc cggtgtccca
      961 ccaaaggtgg tcaattatga agctggtgaa tgggctgaaa actgctacaa tcttgaaatc
     1021 aaaaaacctg acgggagtga gtgtctacca gcagcgccag acgggattcg gggcttcccc
     1081 cggtgccggt atgtgcacaa agtatcagga acgggaccat gtgccggaga ctttgccttc
     1141 cacaaagagg gtgctttctt cctgtatgat cgacttgctt ccacagttat ctaccgagga
     1201 acgactttcg ctgaaggtgt cgttgcattt ctgatactgc cccaagctaa gaaggacttc
     1261 ttcagctcac accccttgag agagccggtc aatgcaacgg aggacccgtc gagtggctat
     1321 tattctacca caattagata tcaggctacc ggttttggaa ctaatgaggc agagtacttg
     1381 ttcgaggttg acaatttgac ctacgtccaa cttgaatcaa gattcacacc acagtttctg
     1441 ctccagctga atgagacaat atatgcaagt gggaagagga gcaacaccac gggaaaacta
     1501 atttggaagg tcaaccccga aattgataca acaatcgggg agtgggcctt ctgggaaact
     1561 aaaaaaaacc tcactagaaa aattcgcagt gaagagttgt ctttcgcagg actgatcaca
     1621 ggcgggagaa ggactcgaag agaagtaatt gtcaatgctc aacccaaatg caaccccaat
     1681 ttacattact ggactactca ggatgaaggt gctgcaatcg gattggcctg gataccatat
     1741 ttcgggccag cagccgaagg aatttacaca gaggggctaa tgcacaacca agatggttta
     1801 atctgtgggt tgaggcagct ggccaacgaa acgactcaag ctctccaact gttcctgaga
     1861 gccacaactg agctgcgaac cttttcaatc ctcaaccgta aggcaattga cttcctgctg
     1921 cagcgatggg gtggcacatg ccacattttg ggaccggact gctgtatcga accacatgat
     1981 tggaccaaga acataacaga caaaattgat cagattattc atgattttgt tgataaaacc
     2041 cttccgggcc agggggacaa tgacaattgg tggacaggat ggagacaatg gataccggca
     2101 ggtattggag ttacaggtgt tataattgca gttatcgctt tattctgtat atgcaaattt
     2161 gtcttttagt ctagagtcgg ggcggccggc cgcttcgagc agacatgata agatacattg
     2221 atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt tgtgaaattt
     2281 gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt aacaacaaca
     2341 attgcattca ttttatgttt caggttcagg gggaggtgtg ggaggttttt taaagcaagt
     2401 aaaacctcta caaatgtggt aaaatcgata aggatccgtc gaccgatgcc cttgagagcc
     2461 ttcaacccag tcagctcctt ccggtgggcg cggggcatga ctatcgtcgc cgcacttatg
     2521 actgtcttct ttatcatgca actcgtagga caggtgccgg cagcgctctt ccgcttcctc
     2581 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
     2641 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     2701 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     2761 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     2821 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     2881 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     2941 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     3001 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     3061 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     3121 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     3181 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     3241 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     3301 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     3361 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
     3421 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
     3481 tatatatgag taaacttggt ctgacagcgg ccgcaaatgc taaaccactg cagtggttac
     3541 cagtgcttga tcagtgaggc accgatctca gcgatctgcc tatttcgttc gtccatagtg
     3601 gcctgactcc ccgtcgtgta gatcactacg attcgtgagg gcttaccatc aggccccagc
     3661 gcagcaatga tgccgcgaga gccgcgttca ccggcccccg atttgtcagc aatgaaccag
     3721 ccagcaggga gggccgagcg aagaagtggt cctgctactt tgtccgcctc catccagtct
     3781 atgagctgct gtcgtgatgc tagagtaaga agttcgccag tgagtagttt ccgaagagtt
     3841 gtggccattg ctactggcat cgtggtatca cgctcgtcgt tcggtatggc ttcgttcaac
     3901 tctggttccc agcggtcaag ccgggtcaca tgatcaccca tattatgaag aaatgcagtc
     3961 agctccttag ggcctccgat cgttgtcaga agtaagttgg ccgcggtgtt gtcgctcatg
     4021 gtaatggcag cactacacaa ttctcttacc gtcatgccat ccgtaagatg cttttccgtg
     4081 accggcgagt actcaaccaa gtcgttttgt gagtagtgta tacggcgacc aagctgctct
     4141 tgcccggcgt ctatacggga caacaccgcg ccacatagca gtactttgaa agtgctcatc
     4201 atcgggaatc gttcttcggg gcggaaagac tcaaggatct tgccgctatt gagatccagt
     4261 tcgatatagc ccactcttgc acccagttga tcttcagcat cttttacttt caccagcgtt
     4321 tcggggtgtg caaaaacagg caagcaaaat gccgcaaaga agggaatgag tgcgacacga
     4381 aaatgttgga tgctcatact cgtccttttt caatattatt gaagcattta tcagggttac
     4441 tagtacgtct ctcaaggata agtaagtaat attaaggtac gggaggtatt ggacaggccg
     4501 caataaaata tctttatttt cattacatct gtgtgttggt tttttgtgtg aatcgatagt
     4561 actaacatac gctctccatc aaaacaaaac gaaacaaaac aaactagcaa aataggctgt
     4621 ccccagtgca agtgcaggtg ccagaacatt tctct
//
