LOCUS Exported 4362 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Gateway Donor vector containing CDK17 , part of the Target Accelerator Plasmid Collection.. ACCESSION . VERSION . KEYWORDS pDONR223_CDK17_p.M85I SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4362) AUTHORS Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn WC TITLE Systematic Functional Interrogation of Rare Cancer Variants Identifies Oncogenic Alleles. JOURNAL Cancer Discov. 2016 Jul;6(7):714-26. doi: 10.1158/2159-8290.CD-16-0160. Epub 2016 May 4. PUBMED 27147599 REFERENCE 2 (bases 1 to 4362) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..4362 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 92..109 /label=L4440 /note="L4440 vector, forward primer" terminator 268..295 /label=rrnB T2 terminator /note="transcription terminator T2 from the E. coli rrnB gene" terminator 387..473 /gene="Escherichia coli rrnB" /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" primer_bind 522..544 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 536..553 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 537..553 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" protein_bind 569..668 /gene="mutant version of attL" /label=attL1 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" protein_bind complement(2241..2340) /gene="mutant version of attL" /label=attL2 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" primer_bind complement(2357..2376) /label=T7 /note="T7 promoter, forward primer" promoter complement(2358..2376) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(2381..2397) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(2381..2397) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" CDS 2828..3619 /codon_start=1 /gene="aadA" /product="aminoglycoside adenylyltransferase (Murphy, 1985)" /label=SmR /note="confers resistance to spectinomycin and streptomycin" /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK" rep_origin 3712..4300 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 4201..4220 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga 61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga 121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca 181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc 241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta 301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc 361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa 421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg 481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa 541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac 601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa 661 agttggcacc atgaaaaaat ttaagagaag gctatccctc acactccgag gaagtcagac 721 tattgatgaa tcattgtctg aattggctga acaaatgact attgaagaaa acagcagcaa 781 ggataatgag cctattgtga agaatggcag gcctccaacg tctcacagta tgcattcctt 841 cctccaccag tacacaggat ctttcaagaa gcccccattg cggagaccac acagtgttat 901 tggagggagc cttggctcct tcatagcaat gcccagaaat ggaagcagat tagatattgt 961 tcatgaaaat ctaaaaatgg gatcagatgg tgagagtgac caagcttctg ggacatcatc 1021 tgatgaagtc cagtcaccta caggtgtttg tctcagaaat cgtatacata gacggatctc 1081 aatggaggat ttaaataagc ggttatcact gcctgcagac atcagaatac ctgatggata 1141 tcttgaaaag ttgcagataa acagtccacc atttgaccaa ccaatgagtc gaaggtctcg 1201 tagagcttcc ttatcagaaa ttggctttgg aaaaatggaa acctacatca aattggaaaa 1261 gcttggagag ggtacatatg caacagtata taaaggaaga agtaaattga cagagaattt 1321 ggtggcatta aaagagatcc gattggaaca tgaagaaggt gcaccctgca cagctataag 1381 agaagtttca ctattaaagg atttaaaaca tgcaaatata gtaaccttac atgacattgt 1441 tcacacagat aaatccttga ctttggtgtt tgagtatctg gataaagacc tgaaacagta 1501 catggatgac tgtggaaaca tcatgagtat gcacaacgta aagctgtttc tgtaccaaat 1561 tctacgtggt ttggcatatt gccatagaag aaaggtattg catcgagact tgaaaccaca 1621 gaacctcctc attaatgaga aaggagaatt aaagctagca gattttggac tagcccgagc 1681 caagtcagtt cccacaaaga cctactcaaa tgaagttgtc acactatggt accggccacc 1741 tgatgtgctt cttggttcct cggagtactc aacacagatt gacatgtggg gtgttggttg 1801 cattttcttt gaaatggctt ctggaagacc tttatttcca ggatcaaccg tggaagatga 1861 actgcactta attttccgac tgctaggaac tccatctcag gaaacttggc caggtatttc 1921 ttcaaatgag gagttcaaga actacaactt tccaaaatat aaaccacagc ctctaattaa 1981 ccacgcaccc aggttagact ctgaaggaat tgagttgata acaaaatttc ttcagtatga 2041 atctaagaaa agggtttcag ctgaagaggc catgaaacat gtgtactttc gaagtctggg 2101 accaagaata catgctttac cagaaagtgt atcaatattc agtttgaaag agattcagtt 2161 gcaaaaggac ccgggttttc gaaattcttc ttatccagag acaggacatg ggaagaacag 2221 aagacagagc atgctctttt acccaacttt cttgtacaaa gttggcatta taagaaagca 2281 ttgcttatca atttgttgca acgaacaggt cactatcagt caaaataaaa tcattatttg 2341 ccatccagct gatatcccct atagtgagtc gtattacatg gtcatagctg tttcctggca 2401 gctctggccc gtgtctcaaa atctctgatg ttacattgca caagataaaa atatatcatc 2461 atgcctcctc tagaccagcc aggacagaaa tgcctcgact tcgctgctac ccaaggttgc 2521 cgggtgacgc acaccgtgga aacggatgaa ggcacgaacc cagtggacat aagcctgttc 2581 ggttcgtaag ctgtaatgca agtagcgtat gcgctcacgc aactggtcca gaaccttgac 2641 cgaacgcagc ggtggtaacg gcgcagtggc ggttttcatg gcttgttatg actgtttttt 2701 tggggtacag tctatgcctc gggcatccaa gcagcaagcg cgttacgccg tgggtcgatg 2761 tttgatgtta tggagcagca acgatgttac gcagcagggc agtcgcccta aaacaaagtt 2821 aaacattatg agggaagcgg tgatcgccga agtatcgact caactatcag aggtagttgg 2881 cgtcatcgag cgccatctcg aaccgacgtt gctggccgta catttgtacg gctccgcagt 2941 ggatggcggc ctgaagccac acagtgatat tgatttgctg gttacggtga ccgtaaggct 3001 tgatgaaaca acgcggcgag ctttgatcaa cgaccttttg gaaacttcgg cttcccctgg 3061 agagagcgag attctccgcg ctgtagaagt caccattgtt gtgcacgacg acatcattcc 3121 gtggcgttat ccagctaagc gcgaactgca atttggagaa tggcagcgca atgacattct 3181 tgcaggtatc ttcgagccag ccacgatcga cattgatctg gctatcttgc tgacaaaagc 3241 aagagaacat agcgttgcct tggtaggtcc agcggcggag gaactctttg atccggttcc 3301 tgaacaggat ctatttgagg cgctaaatga aaccttaacg ctatggaact cgccgcccga 3361 ctgggctggc gatgagcgaa atgtagtgct tacgttgtcc cgcatttggt acagcgcagt 3421 aaccggcaaa atcgcgccga aggatgtcgc tgccgactgg gcaatggagc gcctgccggc 3481 ccagtatcag cccgtcatac ttgaagctag acaggcttat cttggacaag aagaagatcg 3541 cttggcctcg cgcgcagatc agttggaaga atttgtccac tacgtgaaag gcgagatcac 3601 caaggtagtc ggcaaataac cctcgagcca cccatgacca aaatccctta acgtgagtta 3661 cgcgtcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc 3721 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt 3781 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc 3841 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc 3901 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg 3961 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg 4021 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga 4081 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc 4141 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg 4201 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg 4261 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt 4321 tttacggttc ctggcctttt gctggccttt tgctcacatg tt //