LOCUS       Exported                 995 bp ds-DNA     linear   SYN 23-MAY-2021
DEFINITION  In vivo visualization of the mitochondria (can be used for 
            colocalization studies).
ACCESSION   .
VERSION     .
KEYWORDS    pMTS_mScarlet-i_N1
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 995)
  AUTHORS   Bindels DS, Haarbosch L, van Weeren L, Postma M, Wiese KE, Mastop M,
            Aumonier S, Gotthard G, Royant A, Hink MA, Gadella TW Jr
  TITLE     mScarlet: a bright monomeric red fluorescent protein for cellular 
            imaging.
  JOURNAL   Nat Methods. 2016 Nov 21. doi: 10.1038/nmeth.4074.
  PUBMED    27869816
REFERENCE   2  (bases 1 to 995)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 23, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..995
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     54..73
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        54..72
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             92..166
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     regulatory      194..203
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             200..895
                     /codon_start=1
                     /product="bright monomeric red fluorescent protein evolved 
                     from a synthetic template (Bindels et al., 2016)"
                     /label=mScarlet-I
                     /note="matures faster than mScarlet"
                     /translation="MVSKGEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFSWDILSPQFMYGSRAFIKHPADIPDYYKQSFPEGFKWERVMNFEDGG
                     AVTVTQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDGVLKGDIK
                     MALRLKDGGRYLADFKTTYKAKKPVQMPGAYNVDRKLDITSHNEDYTVVEQYERSEGRH
                     STGGMDELYK"
     primer_bind     complement(333..351)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     601..620
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    896..961
                     /label=MCS
                     /note="multiple cloning site"
ORIGIN
        1 agcagagctc tctggctaac tagagaaccc actgcttact ggcttatcga aattaatacg
       61 actcactata gggagaccca agctggctag catgtccgtc ctgacgccgc tgctgctgcg
      121 gggcttgaca ggctcggccc ggcggctccc agtgccgcgc gccaagatcc attcgttggg
      181 ggatccaccg gtcgccacca tggtgagcaa gggcgaggca gtgatcaagg agttcatgcg
      241 gttcaaggtg cacatggagg gctccatgaa cggccacgag ttcgagatcg agggcgaggg
      301 cgagggccgc ccctacgagg gcacccagac cgccaagctg aaggtgacca agggtggccc
      361 cctgcccttc tcctgggaca tcctgtcccc tcagttcatg tacggctcca gggccttcat
      421 caagcacccc gccgacatcc ccgactacta taagcagtcc ttccccgagg gcttcaagtg
      481 ggagcgcgtg atgaacttcg aggacggcgg cgccgtgacc gtgacccagg acacctccct
      541 ggaggacggc accctgatct acaaggtgaa gctccgcggc accaacttcc ctcctgacgg
      601 ccccgtaatg cagaagaaga caatgggctg ggaagcgtcc accgagcggt tgtaccccga
      661 ggacggcgtg ctgaagggcg acattaagat ggccctgcgc ctgaaggacg gcggccgcta
      721 cctggcggac ttcaagacca cctacaaggc caagaagccc gtgcagatgc ccggcgccta
      781 caacgtcgac cgcaagttgg acatcacctc ccacaacgag gactacaccg tggtggaaca
      841 gtacgaacgc tccgagggcc gccactccac cggcggcatg gacgagctgt acaagtccgg
      901 actcagatct cgagctcaag cttcgaattc tgcagtcgac ggtaccgcgg gcccgggatc
      961 caccggatct agataactga tcataatcag ccata
//
