LOCUS Exported 953 bp ds-DNA linear SYN 23-MAY-2021 DEFINITION In vivo visualization of the mitochondria (can be used for colocalization studies). ACCESSION . VERSION . KEYWORDS pMTS_mScarlet-i_N1 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 953) AUTHORS Bindels DS, Haarbosch L, van Weeren L, Postma M, Wiese KE, Mastop M, Aumonier S, Gotthard G, Royant A, Hink MA, Gadella TW Jr TITLE mScarlet: a bright monomeric red fluorescent protein for cellular imaging. JOURNAL Nat Methods. 2016 Nov 21. doi: 10.1038/nmeth.4074. PUBMED 27869816 REFERENCE 2 (bases 1 to 953) AUTHORS . TITLE Direct Submission JOURNAL Exported May 23, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..953 /organism="synthetic DNA construct" /mol_type="other DNA" misc_feature 128..193 /label=MCS /note="multiple cloning site" CDS complement(194..889) /codon_start=1 /product="bright monomeric red fluorescent protein evolved from a synthetic template (Bindels et al., 2016)" /label=mScarlet-I /note="matures faster than mScarlet" /translation="MVSKGEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTA KLKVTKGGPLPFSWDILSPQFMYGSRAFIKHPADIPDYYKQSFPEGFKWERVMNFEDGG AVTVTQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDGVLKGDIK MALRLKDGGRYLADFKTTYKAKKPVQMPGAYNVDRKLDITSHNEDYTVVEQYERSEGRH STGGMDELYK" primer_bind complement(469..488) /label=mCherry-F /note="mCherry, forward primer" primer_bind 738..756 /label=mCherry-R /note="mCherry, reverse primer" regulatory 886..895 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" ORIGIN 1 agttaacaac aacaattgca ttcattttat gtttcaggtt cagggggagg tgtgggaggt 61 tttttaaagc aagtaaaacc tctacaaatg tggtatggct gattatgatc agttatctag 121 atccggtgga tcccgggccc gcggtaccgt cgactgcaga attcgaagct tgagctcgag 181 atctgagtcc ggacttgtac agctcgtcca tgccgccggt ggagtggcgg ccctcggagc 241 gttcgtactg ttccaccacg gtgtagtcct cgttgtggga ggtgatgtcc aacttgcggt 301 cgacgttgta ggcgccgggc atctgcacgg gcttcttggc cttgtaggtg gtcttgaagt 361 ccgccaggta gcggccgccg tccttcaggc gcagggccat cttaatgtcg cccttcagca 421 cgccgtcctc ggggtacaac cgctcggtgg acgcttccca gcccattgtc ttcttctgca 481 ttacggggcc gtcaggaggg aagttggtgc cgcggagctt caccttgtag atcagggtgc 541 cgtcctccag ggaggtgtcc tgggtcacgg tcacggcgcc gccgtcctcg aagttcatca 601 cgcgctccca cttgaagccc tcggggaagg actgcttata gtagtcgggg atgtcggcgg 661 ggtgcttgat gaaggccctg gagccgtaca tgaactgagg ggacaggatg tcccaggaga 721 agggcagggg gccacccttg gtcaccttca gcttggcggt ctgggtgccc tcgtaggggc 781 ggccctcgcc ctcgccctcg atctcgaact cgtggccgtt catggagccc tccatgtgca 841 ccttgaaccg catgaactcc ttgatcactg cctcgccctt gctcaccatg gtggcgaccg 901 gtggatcccc caacgaatgg atcttggcgc gcggcactgg gagccgccgg gcc //