LOCUS       Exported                 999 bp ds-DNA     linear   SYN 17-MAY-2021
DEFINITION  mammalian vector for the expression of a dual read-out BRET-FRET 
            sensor for intracellular Zn2+ imaging.
ACCESSION   .
VERSION     .
KEYWORDS    pCMV-BLZinCh-1
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 999)
  AUTHORS   Aper SJ, Dierickx P, Merkx M
  TITLE     Dual Readout BRET/FRET Sensors for Measuring Intracellular Zinc.
  JOURNAL   ACS Chem Biol. 2016 Oct 21;11(10):2854-2864. Epub 2016 Sep 1.
  PUBMED    27547982
REFERENCE   2  (bases 1 to 999)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 17, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..999
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             complement(73..789)
                     /codon_start=1
                     /product="enhanced variant of YFP (Heikal et al., 2001)"
                     /label=Citrine
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSHLCKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(115..136)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     464..483
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     723..744
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 agcagtaaac ctctacaaat gtggtatggc tgattatgat ctagagtctt agctcgagtg
       61 cggccgcttg tacttgtaca gctcgtccat gccgagagtg atcccggcgg cggtcacgaa
      121 ctccagcagg accatgtgat cgcgcttctc gttggggtct ttgcacaggt gggactggta
      181 gctcaggtag tggttgtcgg gcagcagcac ggggccgtcg ccgatggggg tgttctgctg
      241 gtagtggtcg gcgagctgca cgctgccgtc ctcgatgttg tggcggatct tgaagttcac
      301 cttgatgccg ttcttctgct tgtcggccat gatatagacg ttgtggctgt tgtagttgta
      361 ctcaagcttg tgccccagga tgttgccgtc ctccttgaag tcgatgccct tcagctcgat
      421 gcggttcacc agggtgtcgc cctcgaactt cacctcggcg cgggtcttgt agttgccgtc
      481 gtccttgaag aagatggtgc gctcctggac gtagccttcg ggcatggcgg acttgaagaa
      541 gtcgtgctgc ttcatgtggt cggggtagcg ggcgaagcac atcaggccgt agccgaaggt
      601 ggtcacgagg gtgggccagg gcacgggcag cttgccggtg gtgcagatga acttcagggt
      661 cagcttgccg taggtggcat cgccctcgcc ctcgccggac acgctgaact tgtggccgtt
      721 tacgtcgccg tccagctcga ccaggatggg caccaccccg gtgaacagct cctcgccctt
      781 gctcaccatg ggtaccgagc cccgcggacc cccggatcca ccgctaccac cggatccacc
      841 gcttccaccg gatccaccgc taccaccgga tccaccgctt ccaccggatc cgccgctgcc
      901 gccggatccg ccggagccgc cggatccgcc gctaccgccg gatccgccgc ttccgccgga
      961 tccgccacga atgagctcgc cgcctccgga cttgtacag
//
