LOCUS       Exported                 849 bp ds-DNA     linear   SYN 22-MAY-2021
DEFINITION  lentiviral expression vector for wtGFP S65T.
ACCESSION   .
VERSION     .
KEYWORDS    pGH311_wtGFP S65T
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 849)
  AUTHORS   Hess GT, Fresard L, Han K, Lee CH, Li A, Cimprich KA, Montgomery SB,
            Bassik MC
  TITLE     Directed evolution using dCas9-targeted somatic hypermutation in 
            mammalian cells.
  JOURNAL   Nat Methods. 2016 Oct 31. doi: 10.1038/nmeth.4038.
  PUBMED    27798611
REFERENCE   2  (bases 1 to 849)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 22, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..849
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             21..740
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(66..87)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(327..346)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     674..695
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gtgtcgtgag ctagcccacc atggtgagca agggcgagga gctgttcacc ggggtggtgc
       61 ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tctggcgagg
      121 gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc
      181 tgcccgtgcc ctggcccacc ctcgtgacca ccttcaccta cggcgtgcag tgcttcagcc
      241 gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg
      301 tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga
      361 agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg
      421 acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac gtctatatca
      481 tggccgacaa gcagaagaac ggcatcaagg cgaacttcaa gatccgccac aacatcgagg
      541 acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg
      601 tgctgctgcc cgacaaccac tacctgagca cccagtccgc cctgagcaaa gaccccaacg
      661 agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca
      721 tggacgagct gtacaagtaa tctagagttg aaccgactag taagccaggt cgagacatgt
      781 ttaagggttc cggttccact aggtacaatt cgatatcaag cttatcgata atcaacctct
      841 ggattacaa
//
