LOCUS Exported 815 bp ds-DNA linear SYN 18-MAY-2021 DEFINITION lentiviral expression vector for MS2-AID*Delta fusion protein with hygromycin B selectable marker. ACCESSION . VERSION . KEYWORDS pGH335_MS2-AID*Delta-Hygro SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 815) AUTHORS Hess GT, Fresard L, Han K, Lee CH, Li A, Cimprich KA, Montgomery SB, Bassik MC TITLE Directed evolution using dCas9-targeted somatic hypermutation in mammalian cells. JOURNAL Nat Methods. 2016 Oct 31. doi: 10.1038/nmeth.4038. PUBMED 27798611 REFERENCE 2 (bases 1 to 815) AUTHORS . TITLE Direct Submission JOURNAL Exported May 18, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..815 /organism="synthetic DNA construct" /mol_type="other DNA" CDS 501..554 /codon_start=1 /product="2A peptide from Thosea asigna virus capsid protein" /label=T2A /note="Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides." /translation="EGRGSLLTCGDVEENPGP" ORIGIN 1 agaggcgtga cagtgctaca tccttttcac tggactttgg ttatcttcgc aataagaacg 61 gctgccacgt ggaattgctc ttcctccgct acatctcgga ctgggaccta gaccctggcc 121 gctgctaccg cgtcacctgg ttcatctcct ggagcccctg ctacgactgt gcccgacatg 181 tggccgactt tctgcgaggg aaccccaacc tcagtctgag gatcttcacc gcgcgcctct 241 acttctgtga ggaccgcaag gctgagcccg aggggctgcg gcggctgcac cgcgccgggg 301 tgcaaatagc catcatgacc ttcaaagatt atttttactg ctggaatact tttgtagaaa 361 accacggaag aactttcaaa gcctgggaag ggctgcatga aaattcagtt cgtctctcca 421 gacagcttcg gcgcatcctt ttgcccctgt atgaggttga tgacttacga gacgcatttc 481 gtacttgtac aggcagtgga gagggcagag gaagtctgct aacatgcggt gacgtcgagg 541 agaatcctgg cccaaccatg aaaaagcctg aactcaccgc tacctctgtc gagaagtttc 601 tgatcgaaaa gttcgacagc gtctccgacc tgatgcagct ctccgagggc gaagaatctc 661 gggctttcag cttcgatgtg ggagggcgtg gatatgtcct gcgggtgaat agctgcgccg 721 atggtttcta caaagatcgc tatgtttatc ggcactttgc atccgccgct ctccctattc 781 ccgaagtgct tgacattggg gagttcagcg agagc //