LOCUS Exported 3873 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Gateway Donor vector containing WNT2 , part of the Target Accelerator Plasmid Collection.. ACCESSION . VERSION . KEYWORDS pDONR223_WNT2_p.V334M SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3873) AUTHORS Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn WC TITLE Systematic Functional Interrogation of Rare Cancer Variants Identifies Oncogenic Alleles. JOURNAL Cancer Discov. 2016 Jul;6(7):714-26. doi: 10.1158/2159-8290.CD-16-0160. Epub 2016 May 4. PUBMED 27147599 REFERENCE 2 (bases 1 to 3873) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..3873 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 43..65 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 57..74 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 58..74 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" protein_bind 90..189 /gene="mutant version of attL" /label=attL1 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" protein_bind complement(1273..1372) /gene="mutant version of attL" /label=attL2 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" primer_bind complement(1389..1408) /label=T7 /note="T7 promoter, forward primer" promoter complement(1390..1408) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(1413..1429) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(1413..1429) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" CDS 1860..2651 /codon_start=1 /gene="aadA" /product="aminoglycoside adenylyltransferase (Murphy, 1985)" /label=SmR /note="confers resistance to spectinomycin and streptomycin" /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK" rep_origin 2744..3332 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 3233..3252 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 3486..3503 /label=L4440 /note="L4440 vector, forward primer" terminator 3662..3689 /label=rrnB T2 terminator /note="transcription terminator T2 from the E. coli rrnB gene" terminator 3781..3867 /gene="Escherichia coli rrnB" /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" ORIGIN 1 ggcagttccc tactctcgcg ttaacgctag catggatgtt ttcccagtca cgacgttgta 61 aaacgacggc cagtcttaag ctcgggcccc aaataatgat tttattttga ctgatagtga 121 cctgttcgtt gcaacaaatt gatgagcaat gcttttttat aatgccaact ttgtacaaaa 181 aagttggcat gaacgcccct ctcggtggaa tctggctctg gctccctctg ctcttgacct 241 ggctcacccc agaggtcaac tcttcatggt ggtacatgag agctacaggt ggctcctcca 301 gggtgatgtg cgataatgtg ccaggcctgg tgagcagcca gcggcagctg tgtcaccgac 361 atccagatgt gatgcgtgcc attagccagg gcgtggccga gtggacagca gaatgccagc 421 accagttccg ccagcaccgc tggaattgca acaccctgga cagggatcac agcctttttg 481 gcagggtcct actccgaagt agtcgggaat ctgcctttgt ttatgccatc tcctcagctg 541 gagttgtatt tgccatcacc agggcctgta gccaaggaga agtaaaatcc tgttcctgtg 601 atccaaagaa gatgggaagc gccaaggaca gcaaaggcat ttttgattgg ggtggctgca 661 gtgataacat tgactatggg atcaaatttg cccgcgcatt tgtggatgca aaggaaagga 721 aaggaaagga tgccagagcc ctgatgaatc ttcacaacaa cagagctggc aggaaggctg 781 taaagcggtt cttgaaacaa gagtgcaagt gccacggggt gagcggctca tgtactctca 841 ggacatgctg gctggccatg gccgacttca ggaaaacggg cgattatctc tggaggaagt 901 acaatggggc catccaggtg gtcatgaacc aggatggcac aggtttcact gtggctaacg 961 agaggtttaa gaagccaacg aaaaatgacc tcgtgtattt tgagaattct ccagactact 1021 gtatcaggga ccgagaggca ggctccctgg gtacagcagg ccgtgtgtgc aacctgactt 1081 cccggggcat ggacagctgt gaagtcatgt gctgtgggag aggctacgac acctcccatg 1141 tcacccggat gaccaagtgt gggtgtaagt tccactggtg ctgcgccatg cgctgtcagg 1201 actgcctgga agctctggat gtgcacacat gcaaggcccc caagaacgct gactggacaa 1261 ccgctacatg attgccaact ttcttgtaca aagttggcat tataagaaag cattgcttat 1321 caatttgttg caacgaacag gtcactatca gtcaaaataa aatcattatt tgccatccag 1381 ctgatatccc ctatagtgag tcgtattaca tggtcatagc tgtttcctgg cagctctggc 1441 ccgtgtctca aaatctctga tgttacattg cacaagataa aaatatatca tcatgcctcc 1501 tctagaccag ccaggacaga aatgcctcga cttcgctgct acccaaggtt gccgggtgac 1561 gcacaccgtg gaaacggatg aaggcacgaa cccagtggac ataagcctgt tcggttcgta 1621 agctgtaatg caagtagcgt atgcgctcac gcaactggtc cagaaccttg accgaacgca 1681 gcggtggtaa cggcgcagtg gcggttttca tggcttgtta tgactgtttt tttggggtac 1741 agtctatgcc tcgggcatcc aagcagcaag cgcgttacgc cgtgggtcga tgtttgatgt 1801 tatggagcag caacgatgtt acgcagcagg gcagtcgccc taaaacaaag ttaaacatta 1861 tgagggaagc ggtgatcgcc gaagtatcga ctcaactatc agaggtagtt ggcgtcatcg 1921 agcgccatct cgaaccgacg ttgctggccg tacatttgta cggctccgca gtggatggcg 1981 gcctgaagcc acacagtgat attgatttgc tggttacggt gaccgtaagg cttgatgaaa 2041 caacgcggcg agctttgatc aacgaccttt tggaaacttc ggcttcccct ggagagagcg 2101 agattctccg cgctgtagaa gtcaccattg ttgtgcacga cgacatcatt ccgtggcgtt 2161 atccagctaa gcgcgaactg caatttggag aatggcagcg caatgacatt cttgcaggta 2221 tcttcgagcc agccacgatc gacattgatc tggctatctt gctgacaaaa gcaagagaac 2281 atagcgttgc cttggtaggt ccagcggcgg aggaactctt tgatccggtt cctgaacagg 2341 atctatttga ggcgctaaat gaaaccttaa cgctatggaa ctcgccgccc gactgggctg 2401 gcgatgagcg aaatgtagtg cttacgttgt cccgcatttg gtacagcgca gtaaccggca 2461 aaatcgcgcc gaaggatgtc gctgccgact gggcaatgga gcgcctgccg gcccagtatc 2521 agcccgtcat acttgaagct agacaggctt atcttggaca agaagaagat cgcttggcct 2581 cgcgcgcaga tcagttggaa gaatttgtcc actacgtgaa aggcgagatc accaaggtag 2641 tcggcaaata accctcgagc cacccatgac caaaatccct taacgtgagt tacgcgtcgt 2701 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc 2761 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc 2821 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac 2881 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac 2941 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt 3001 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct 3061 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat 3121 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt 3181 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg 3241 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt 3301 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt 3361 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg 3421 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg 3481 agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc 3541 ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg 3601 gcagtgagcg caacgcaatt aatacgcgta ccgctagcca ggaagagttt gtagaaacgc 3661 aaaaaggcca tccgtcagga tggccttctg cttagtttga tgcctggcag tttatggcgg 3721 gcgtcctgcc cgccaccctc cgggccgttg cttcacaacg ttcaaatccg ctcccggcgg 3781 atttgtccta ctcaggagag cgttcaccga caaacaacag ataaaacgaa aggcccagtc 3841 ttccgactga gcctttcgtt ttatttgatg cct //