LOCUS       Exported                 707 bp ds-DNA     linear   SYN 26-MAY-2021
DEFINITION  Expresses Mj aaRS for sulfotyrosine and Mj tRNA for decoding UAG 
            codons.
ACCESSION   .
VERSION     .
KEYWORDS    pUltra-sY
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 707)
  AUTHORS   Schwessinger B, Li X, Ellinghaus TL, Chan LJ, Wei T, Joe A, Thomas 
            N, Pruitt R, Adams PD, Chern MS, Petzold CJ, Liu CC, Ronald PC
  TITLE     A second-generation expression system for tyrosine-sulfated proteins
            and its application in crop protection.
  JOURNAL   Integr Biol (Camb). 2016 Apr 18;8(4):542-5. doi: 10.1039/c5ib00232j.
            Epub 2015 Nov 27.
  PUBMED    26611838
REFERENCE   2  (bases 1 to 707)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 26, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..707
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        112..140
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    148..164
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 attgactctc ttccgggcgc tatcatgcca taccgcgaaa ggttttgcgc cattcgatgg
       61 tgtccgggat ctcgacgctc tcccttatgc gactcctgca ttagggagct gttgacaatt
      121 aatcatcggc tcgtataatg tgtggaattg tgagcggata acaatttcac aaaggaggtg
      181 cggccgcatg gacgaatttg aaatgataaa gagaaacaca tctgaaatta tcagcgagga
      241 agagttaaga gaggttttaa aaaaagatga aaaatctgct ctgataggtt ttgaaccaag
      301 tggtaaaata catttagggc attatctcca aataaaaaag atgattgatt tacaaaatgc
      361 tggatttgat ataattatac ctttggctga tttacatgcc tatttaaacc agaaaggaga
      421 gttggatgag attagaaaaa taggagatta taacaaaaaa gtttttgaag caatggggtt
      481 aaaggcaaaa tatgtttatg gaagtgaatt ccagcttgat aaggattata cactgaatgt
      541 ctatagattg gctttaaaaa ctaccttaaa aagagcaaga aggagtatgg aacttatagc
      601 aagagaggat gaaaatccaa aggttgctga agttatctat ccaataatgc aggttaatgg
      661 ttgtcattat aagggcgttg atgttgcagt tggagggatg gagcaga
//
