LOCUS Exported 3689 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Can be used for in situ hybridisation or other purposes. ACCESSION . VERSION . KEYWORDS Rab4a cds in pGem-T Easy SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3689) AUTHORS Hall TE, Martel N, Lo HP, Xiong Z, Parton RG TITLE A plasmid library of full-length zebrafish rab proteins for in vivo cell biology. JOURNAL Cell Logist. 2017 Mar 1;7(1):e1301151. doi: 10.1080/21592799.2017.1301151. eCollection 2017. PUBMED 28396820 REFERENCE 2 (bases 1 to 3689) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..3689 /organism="synthetic DNA construct" /mol_type="other DNA" promoter 210..314 /gene="bla" /label=AmpR promoter CDS 315..1175 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(533..552) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 1346..1934 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 1835..1854 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 2088..2105 /label=L4440 /note="L4440 vector, forward primer" protein_bind 2222..2243 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 2258..2288 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 2296..2312 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 2301..2323 /label=M13/pUC Reverse /note="In lacZ gene" primer_bind 2320..2336 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 2320..2336 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" promoter 2354..2372 /label=SP6 promoter /note="promoter for bacteriophage SP6 RNA polymerase" primer_bind 2354..2371 /label=SP6 /note="SP6 promoter, forward primer" regulatory 3140..3149 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" regulatory 3144..3153 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" primer_bind complement(3183..3202) /label=T7 /note="T7 promoter, forward primer" promoter complement(3184..3202) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(3209..3226) /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind complement(3209..3225) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind complement(3218..3240) /label=M13/pUC Forward /note="In lacZ gene" rep_origin 3366..132 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(3453..3472) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 3663..3684 /label=F1ori-F /note="F1 origin, forward primer" ORIGIN 1 cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct 61 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa 121 cgcttacaat ttcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg 181 catcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa 241 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt 301 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg 361 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag 421 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg 481 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg 541 gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt 601 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga 661 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac 721 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc 781 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc 841 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac 901 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag 961 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg 1021 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta 1081 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg 1141 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata 1201 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt 1261 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc 1321 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct 1381 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa 1441 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag 1501 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc 1561 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg 1621 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca 1681 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat 1741 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg 1801 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc 1861 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc 1921 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc 1981 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg 2041 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga 2101 gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc 2161 attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa 2221 ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc 2281 gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg 2341 attacgccaa gctatttagg tgacactata gaatactcaa gctatgcatc caacgcgttg 2401 ggagctctcc catatggtcg acctgcaggc ggccgcgaat tcactagtga ttagcgccag 2461 tcatgtcaga gacatacgat ttcttgttta agtttcttgt gattgggaat gctggaacag 2521 gaaaatcctg tcttcttcat cagttcatag aaaaaagatt caaagatgac tcaaatcaca 2581 ctattggggt tgaatttggt tcgaagataa tcagcgtggt caataagttt gtaaagctcc 2641 agatctggga cacagcagga caggagcgtt tcaggtctgt aactaggagt tattacagag 2701 gcgcagcagg ggctcttcta gtctatgaca tcactagtcg agaaacatac aacgctctaa 2761 ccaactggct gacagatgcc cggatgttgg ccagtcagaa catagtgatc atactatgtg 2821 gaaacaagaa ggatctggat gctgaccgcg aggtgacctt tctggaggct tcacgcttcg 2881 ctcaggagaa tgagcttatg tttttggaga ccagtgctct gacaggggag aatgtggagg 2941 aagctttcgt ccagtgtgct cgaaaaatcc ttaacaaaat tgaatcagga gagctggacc 3001 cagagcggat gggttcagga atccagtatg gagacgcggc tcttcgacag ctgcgttcac 3061 ccagacgggc acaagccgag agcatccagg agtgtggctg ttaacacacg tccctctgaa 3121 cagaaatcga attcccgcgg ccgccatggc ggccgggagc atgcgacgtc gggcccaatt 3181 cgccctatag tgagtcgtat tacaattcac tggccgtcgt tttacaacgt cgtgactggg 3241 aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc 3301 gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg 3361 aatggacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt 3421 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct 3481 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg 3541 atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag 3601 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa 3661 tagtggactc ttgttccaaa ctggaacaa //