LOCUS Exported 9294 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Encodes gRNA for 3' target of human NFIL3. ACCESSION . VERSION . KEYWORDS PX458_NFIL3_1 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 9294) AUTHORS Savic D, Partridge EC, Newberry KM, Smith SB, Meadows SK, Roberts BS, Mackiewicz M, Mendenhall EM, Myers RM TITLE CETCh-seq: CRISPR epitope tagging ChIP-seq of DNA-binding proteins. JOURNAL Genome Res. 2015 Sep 9. PUBMED 26355004 REFERENCE 2 (bases 1 to 9294) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..9294 /organism="synthetic DNA construct" /mol_type="other DNA" promoter 17..257 /label=U6 promoter /note="RNA polymerase III promoter for human U6 snRNA" primer_bind 17..37 /label=hU6-F /note="Human U6 promoter, forward primer" primer_bind 188..207 /label=LKO.1 5' /note="Human U6 promoter, forward primer" misc_RNA 287..362 /label=gRNA scaffold /note="guide RNA scaffold for the Streptococcus pyogenes CRISPR/Cas9 system" enhancer 459..744 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer; contains an 18-bp deletion relative to the standard CMV enhancer" promoter 746..1023 /label=chicken beta-actin promoter intron 1024..1252 /label=hybrid intron /note="hybrid between chicken beta-actin (CBA) and minute virus of mice (MMV) introns (Gray et al., 2011)" regulatory 1264..1273 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 1273..1338 /codon_start=1 /product="three tandem FLAG(R) epitope tags, followed by an enterokinase cleavage site" /label=3xFLAG /translation="DYKDHDGDYKDHDIDYKDDDDK" CDS 1345..1365 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" CDS 1390..5490 /codon_start=1 /product="Cas9 (Csn1) endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system" /label=Cas9 /note="generates RNA-guided double strand breaks in DNA" /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD ATLIHQSITGLYETRIDLSQLGGD" CDS 5491..5538 /codon_start=1 /product="bipartite nuclear localization signal from nucleoplasmin" /label=nucleoplasmin NLS /translation="KRPAATKKAGQAKKKK" CDS 5554..5607 /codon_start=1 /product="2A peptide from Thosea asigna virus capsid protein" /label=T2A /note="Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides." /translation="EGRGSLLTCGDVEENPGP" CDS 5608..6321 /codon_start=1 /product="enhanced GFP" /label=EGFP /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE FVTAAGITLGMDELYK" CDS 5608..6321 /codon_start=1 /product="the original enhanced GFP (Yang et al., 1996)" /label=EGFP /note="mammalian codon-optimized" /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE FVTAAGITLGMDELYK" primer_bind complement(5650..5671) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(5911..5930) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 6258..6279 /label=EGFP-C /note="EGFP, forward primer" primer_bind complement(6349..6366) /label=BGH-rev /note="Bovine growth hormone terminator, reverse primer. Also called BGH reverse" polyA_signal 6355..6562 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" repeat_region 6571..6711 /label=AAV2 ITR /note="inverted terminal repeat of adeno-associated virus serotype 2" repeat_region 6571..6700 /label=AAV2 ITR rep_origin 6786..7241 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(6873..6892) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 7083..7104 /label=F1ori-F /note="F1 origin, forward primer" primer_bind complement(7258..7277) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind 7377..7399 /label=pGEX 3' /note="pGEX vectors, reverse primer" primer_bind complement(7437..7455) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 7523..7627 /gene="bla" /label=AmpR promoter CDS 7628..8488 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(7846..7865) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 8659..9247 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 9148..9167 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 ttttgctcac atgtnngagg gcctatttcc catgattcct tcatatttgc atatacgata 61 caaggctgtt agagagataa ttggaattaa tttgactgta aacacaaaga tattagtaca 121 aaatacgtga cgtagaaagt aataatttct tgggtagttt gcagttttaa aattatgttt 181 taaaatggac tatcatatgc ttaccgtaac ttgaaagtat ttcgatttct tggctttata 241 tatcttgtgg aaaggacgaa acaccgatta ctactgagta agagctgttt tagagctaga 301 aatagcaagt taaaataagg ctagtccgtt atcaacttga aaaagtggca ccgagtcggt 361 gcttttttgt tttagagcta gaaatagcaa gttaaaataa ggctagtccg tttttagcgc 421 gtgcgccaat tctgcagaca aatggctcta gaggtacccg ttacataact tacggtaaat 481 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaatagt aacgccaata 541 gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta 601 catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc 661 gcctggcatt gtgcccagta catgacctta tgggactttc ctacttggca gtacatctac 721 gtattagtca tcgctattac catggtcgag gtgagcccca cgttctgctt cactctcccc 781 atctcccccc cctccccacc cccaattttg tatttattta ttttttaatt attttgtgca 841 gcgatggggg cggggggggg gggggggcgc gcgccaggcg gggcggggcg gggcgagggg 901 cggggcgggg cgaggcggag aggtgcggcg gcagccaatc agagcggcgc gctccgaaag 961 tttcctttta tggcgaggcg gcggcggcgg cggccctata aaaagcgaag cgcgcggcgg 1021 gcgggagtcg ctgcgacgct gccttcgccc cgtgccccgc tccgccgccg cctcgcgccg 1081 cccgccccgg ctctgactga ccgcgttact cccacaggtg agcgggcggg acggcccttc 1141 tcctccgggc tgtaattagc tgagcaagag gtaagggttt aagggatggt tggttggtgg 1201 ggtattaatg tttaattacc tggagcacct gcctgaaatc actttttttc aggttggacc 1261 ggtgccacca tggactataa ggaccacgac ggagactaca aggatcatga tattgattac 1321 aaagacgatg acgataagat ggccccaaag aagaagcgga aggtcggtat ccacggagtc 1381 ccagcagccg acaagaagta cagcatcggc ctggacatcg gcaccaactc tgtgggctgg 1441 gccgtgatca ccgacgagta caaggtgccc agcaagaaat tcaaggtgct gggcaacacc 1501 gaccggcaca gcatcaagaa gaacctgatc ggagccctgc tgttcgacag cggcgaaaca 1561 gccgaggcca cccggctgaa gagaaccgcc agaagaagat acaccagacg gaagaaccgg 1621 atctgctatc tgcaagagat cttcagcaac gagatggcca aggtggacga cagcttcttc 1681 cacagactgg aagagtcctt cctggtggaa gaggataaga agcacgagcg gcaccccatc 1741 ttcggcaaca tcgtggacga ggtggcctac cacgagaagt accccaccat ctaccacctg 1801 agaaagaaac tggtggacag caccgacaag gccgacctgc ggctgatcta tctggccctg 1861 gcccacatga tcaagttccg gggccacttc ctgatcgagg gcgacctgaa ccccgacaac 1921 agcgacgtgg acaagctgtt catccagctg gtgcagacct acaaccagct gttcgaggaa 1981 aaccccatca acgccagcgg cgtggacgcc aaggccatcc tgtctgccag actgagcaag 2041 agcagacggc tggaaaatct gatcgcccag ctgcccggcg agaagaagaa tggcctgttc 2101 ggaaacctga ttgccctgag cctgggcctg acccccaact tcaagagcaa cttcgacctg 2161 gccgaggatg ccaaactgca gctgagcaag gacacctacg acgacgacct ggacaacctg 2221 ctggcccaga tcggcgacca gtacgccgac ctgtttctgg ccgccaagaa cctgtccgac 2281 gccatcctgc tgagcgacat cctgagagtg aacaccgaga tcaccaaggc ccccctgagc 2341 gcctctatga tcaagagata cgacgagcac caccaggacc tgaccctgct gaaagctctc 2401 gtgcggcagc agctgcctga gaagtacaaa gagattttct tcgaccagag caagaacggc 2461 tacgccggct acattgacgg cggagccagc caggaagagt tctacaagtt catcaagccc 2521 atcctggaaa agatggacgg caccgaggaa ctgctcgtga agctgaacag agaggacctg 2581 ctgcggaagc agcggacctt cgacaacggc agcatccccc accagatcca cctgggagag 2641 ctgcacgcca ttctgcggcg gcaggaagat ttttacccat tcctgaagga caaccgggaa 2701 aagatcgaga agatcctgac cttccgcatc ccctactacg tgggccctct ggccagggga 2761 aacagcagat tcgcctggat gaccagaaag agcgaggaaa ccatcacccc ctggaacttc 2821 gaggaagtgg tggacaaggg cgcttccgcc cagagcttca tcgagcggat gaccaacttc 2881 gataagaacc tgcccaacga gaaggtgctg cccaagcaca gcctgctgta cgagtacttc 2941 accgtgtata acgagctgac caaagtgaaa tacgtgaccg agggaatgag aaagcccgcc 3001 ttcctgagcg gcgagcagaa aaaggccatc gtggacctgc tgttcaagac caaccggaaa 3061 gtgaccgtga agcagctgaa agaggactac ttcaagaaaa tcgagtgctt cgactccgtg 3121 gaaatctccg gcgtggaaga tcggttcaac gcctccctgg gcacatacca cgatctgctg 3181 aaaattatca aggacaagga cttcctggac aatgaggaaa acgaggacat tctggaagat 3241 atcgtgctga ccctgacact gtttgaggac agagagatga tcgaggaacg gctgaaaacc 3301 tatgcccacc tgttcgacga caaagtgatg aagcagctga agcggcggag atacaccggc 3361 tggggcaggc tgagccggaa gctgatcaac ggcatccggg acaagcagtc cggcaagaca 3421 atcctggatt tcctgaagtc cgacggcttc gccaacagaa acttcatgca gctgatccac 3481 gacgacagcc tgacctttaa agaggacatc cagaaagccc aggtgtccgg ccagggcgat 3541 agcctgcacg agcacattgc caatctggcc ggcagccccg ccattaagaa gggcatcctg 3601 cagacagtga aggtggtgga cgagctcgtg aaagtgatgg gccggcacaa gcccgagaac 3661 atcgtgatcg aaatggccag agagaaccag accacccaga agggacagaa gaacagccgc 3721 gagagaatga agcggatcga agagggcatc aaagagctgg gcagccagat cctgaaagaa 3781 caccccgtgg aaaacaccca gctgcagaac gagaagctgt acctgtacta cctgcagaat 3841 gggcgggata tgtacgtgga ccaggaactg gacatcaacc ggctgtccga ctacgatgtg 3901 gaccatatcg tgcctcagag ctttctgaag gacgactcca tcgacaacaa ggtgctgacc 3961 agaagcgaca agaaccgggg caagagcgac aacgtgccct ccgaagaggt cgtgaagaag 4021 atgaagaact actggcggca gctgctgaac gccaagctga ttacccagag aaagttcgac 4081 aatctgacca aggccgagag aggcggcctg agcgaactgg ataaggccgg cttcatcaag 4141 agacagctgg tggaaacccg gcagatcaca aagcacgtgg cacagatcct ggactcccgg 4201 atgaacacta agtacgacga gaatgacaag ctgatccggg aagtgaaagt gatcaccctg 4261 aagtccaagc tggtgtccga tttccggaag gatttccagt tttacaaagt gcgcgagatc 4321 aacaactacc accacgccca cgacgcctac ctgaacgccg tcgtgggaac cgccctgatc 4381 aaaaagtacc ctaagctgga aagcgagttc gtgtacggcg actacaaggt gtacgacgtg 4441 cggaagatga tcgccaagag cgagcaggaa atcggcaagg ctaccgccaa gtacttcttc 4501 tacagcaaca tcatgaactt tttcaagacc gagattaccc tggccaacgg cgagatccgg 4561 aagcggcctc tgatcgagac aaacggcgaa accggggaga tcgtgtggga taagggccgg 4621 gattttgcca ccgtgcggaa agtgctgagc atgccccaag tgaatatcgt gaaaaagacc 4681 gaggtgcaga caggcggctt cagcaaagag tctatcctgc ccaagaggaa cagcgataag 4741 ctgatcgcca gaaagaagga ctgggaccct aagaagtacg gcggcttcga cagccccacc 4801 gtggcctatt ctgtgctggt ggtggccaaa gtggaaaagg gcaagtccaa gaaactgaag 4861 agtgtgaaag agctgctggg gatcaccatc atggaaagaa gcagcttcga gaagaatccc 4921 atcgactttc tggaagccaa gggctacaaa gaagtgaaaa aggacctgat catcaagctg 4981 cctaagtact ccctgttcga gctggaaaac ggccggaaga gaatgctggc ctctgccggc 5041 gaactgcaga agggaaacga actggccctg ccctccaaat atgtgaactt cctgtacctg 5101 gccagccact atgagaagct gaagggctcc cccgaggata atgagcagaa acagctgttt 5161 gtggaacagc acaagcacta cctggacgag atcatcgagc agatcagcga gttctccaag 5221 agagtgatcc tggccgacgc taatctggac aaagtgctgt ccgcctacaa caagcaccgg 5281 gataagccca tcagagagca ggccgagaat atcatccacc tgtttaccct gaccaatctg 5341 ggagcccctg ccgccttcaa gtactttgac accaccatcg accggaagag gtacaccagc 5401 accaaagagg tgctggacgc caccctgatc caccagagca tcaccggcct gtacgagaca 5461 cggatcgacc tgtctcagct gggaggcgac aaaaggccgg cggccacgaa aaaggccggc 5521 caggcaaaaa agaaaaagga attcggcagt ggagagggca gaggaagtct gctaacatgc 5581 ggtgacgtcg aggagaatcc tggcccagtg agcaagggcg aggagctgtt caccggggtg 5641 gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc 5701 gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc 5761 aagctgcccg tgccctggcc caccctcgtg accaccctga cctacggcgt gcagtgcttc 5821 agccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc 5881 tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag 5941 gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag 6001 gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat 6061 atcatggccg acaagcagaa gaacggcatc aaggtgaact tcaagatccg ccacaacatc 6121 gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc 6181 cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccgccctgag caaagacccc 6241 aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc 6301 ggcatggacg agctgtacaa ggaattctaa ctagagctcg ctgatcagcc tcgactgtgc 6361 cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag 6421 gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta 6481 ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag 6541 agaatagcag gcatgctggg gagcggccgc aggaacccct agtgatggag ttggccactc 6601 cctctctgcg cgctcgctcg ctcactgagg ccgggcgacc aaaggtcgcc cgacgcccgg 6661 gctttgcccg ggcggcctca gtgagcgagc gagcgcgcag ctgcctgcag gggcgcctga 6721 tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatacgt caaagcaacc 6781 atagtacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt 6841 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct 6901 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg 6961 atttagtgct ttacggcacc tcgaccccaa aaaacttgat ttgggtgatg gttcacgtag 7021 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa 7081 tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcgggct attcttttga 7141 tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa 7201 atttaacgcg aattttaaca aaatattaac gtttacaatt ttatggtgca ctctcagtac 7261 aatctgctct gatgccgcat agttaagcca gccccgacac ccgccaacac ccgctgacgc 7321 gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg 7381 gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct 7441 cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg 7501 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc 7561 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag 7621 gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg 7681 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt 7741 gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt 7801 tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt 7861 attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa 7921 tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag 7981 agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac 8041 aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac 8101 tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac 8161 cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac 8221 tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact 8281 tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg 8341 tggaagccgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt 8401 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat 8461 aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta 8521 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa 8581 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga 8641 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac 8701 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt 8761 tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc 8821 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat 8881 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag 8941 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc 9001 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag 9061 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac 9121 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg 9181 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct 9241 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggcc //