LOCUS Exported 9293 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Encodes gRNA for 3' target of human SP5. ACCESSION . VERSION . KEYWORDS PX458_SP5_1 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 9293) AUTHORS Savic D, Partridge EC, Newberry KM, Smith SB, Meadows SK, Roberts BS, Mackiewicz M, Mendenhall EM, Myers RM TITLE CETCh-seq: CRISPR epitope tagging ChIP-seq of DNA-binding proteins. JOURNAL Genome Res. 2015 Sep 9. PUBMED 26355004 REFERENCE 2 (bases 1 to 9293) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..9293 /organism="synthetic DNA construct" /mol_type="other DNA" promoter 17..257 /label=U6 promoter /note="RNA polymerase III promoter for human U6 snRNA" primer_bind 17..37 /label=hU6-F /note="Human U6 promoter, forward primer" primer_bind 188..207 /label=LKO.1 5' /note="Human U6 promoter, forward primer" misc_RNA 286..361 /label=gRNA scaffold /note="guide RNA scaffold for the Streptococcus pyogenes CRISPR/Cas9 system" enhancer 458..743 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer; contains an 18-bp deletion relative to the standard CMV enhancer" promoter 745..1022 /label=chicken beta-actin promoter intron 1023..1251 /label=hybrid intron /note="hybrid between chicken beta-actin (CBA) and minute virus of mice (MMV) introns (Gray et al., 2011)" regulatory 1263..1272 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 1272..1337 /codon_start=1 /product="three tandem FLAG(R) epitope tags, followed by an enterokinase cleavage site" /label=3xFLAG /translation="DYKDHDGDYKDHDIDYKDDDDK" CDS 1344..1364 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" CDS 1389..5489 /codon_start=1 /product="Cas9 (Csn1) endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system" /label=Cas9 /note="generates RNA-guided double strand breaks in DNA" /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD ATLIHQSITGLYETRIDLSQLGGD" CDS 5490..5537 /codon_start=1 /product="bipartite nuclear localization signal from nucleoplasmin" /label=nucleoplasmin NLS /translation="KRPAATKKAGQAKKKK" CDS 5553..5606 /codon_start=1 /product="2A peptide from Thosea asigna virus capsid protein" /label=T2A /note="Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides." /translation="EGRGSLLTCGDVEENPGP" CDS 5607..6320 /codon_start=1 /product="enhanced GFP" /label=EGFP /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE FVTAAGITLGMDELYK" CDS 5607..6320 /codon_start=1 /product="the original enhanced GFP (Yang et al., 1996)" /label=EGFP /note="mammalian codon-optimized" /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE FVTAAGITLGMDELYK" primer_bind complement(5649..5670) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(5910..5929) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 6257..6278 /label=EGFP-C /note="EGFP, forward primer" primer_bind complement(6348..6365) /label=BGH-rev /note="Bovine growth hormone terminator, reverse primer. Also called BGH reverse" polyA_signal 6354..6561 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" repeat_region 6570..6710 /label=AAV2 ITR /note="inverted terminal repeat of adeno-associated virus serotype 2" repeat_region 6570..6699 /label=AAV2 ITR rep_origin 6785..7240 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(6872..6891) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 7082..7103 /label=F1ori-F /note="F1 origin, forward primer" primer_bind complement(7257..7276) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind 7376..7398 /label=pGEX 3' /note="pGEX vectors, reverse primer" primer_bind complement(7436..7454) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 7522..7626 /gene="bla" /label=AmpR promoter CDS 7627..8487 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(7845..7864) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 8658..9246 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 9147..9166 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 ttttgctcac atgtnngagg gcctatttcc catgattcct tcatatttgc atatacgata 61 caaggctgtt agagagataa ttggaattaa tttgactgta aacacaaaga tattagtaca 121 aaatacgtga cgtagaaagt aataatttct tgggtagttt gcagttttaa aattatgttt 181 taaaatggac tatcatatgc ttaccgtaac ttgaaagtat ttcgatttct tggctttata 241 tatcttgtgg aaaggacgaa acaccggacc tgtgagccct cccgggtttt agagctagaa 301 atagcaagtt aaaataaggc tagtccgtta tcaacttgaa aaagtggcac cgagtcggtg 361 cttttttgtt ttagagctag aaatagcaag ttaaaataag gctagtccgt ttttagcgcg 421 tgcgccaatt ctgcagacaa atggctctag aggtacccgt tacataactt acggtaaatg 481 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaatagta acgccaatag 541 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac 601 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg 661 cctggcattg tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg 721 tattagtcat cgctattacc atggtcgagg tgagccccac gttctgcttc actctcccca 781 tctccccccc ctccccaccc ccaattttgt atttatttat tttttaatta ttttgtgcag 841 cgatgggggc gggggggggg ggggggcgcg cgccaggcgg ggcggggcgg ggcgaggggc 901 ggggcggggc gaggcggaga ggtgcggcgg cagccaatca gagcggcgcg ctccgaaagt 961 ttccttttat ggcgaggcgg cggcggcggc ggccctataa aaagcgaagc gcgcggcggg 1021 cgggagtcgc tgcgacgctg ccttcgcccc gtgccccgct ccgccgccgc ctcgcgccgc 1081 ccgccccggc tctgactgac cgcgttactc ccacaggtga gcgggcggga cggcccttct 1141 cctccgggct gtaattagct gagcaagagg taagggttta agggatggtt ggttggtggg 1201 gtattaatgt ttaattacct ggagcacctg cctgaaatca ctttttttca ggttggaccg 1261 gtgccaccat ggactataag gaccacgacg gagactacaa ggatcatgat attgattaca 1321 aagacgatga cgataagatg gccccaaaga agaagcggaa ggtcggtatc cacggagtcc 1381 cagcagccga caagaagtac agcatcggcc tggacatcgg caccaactct gtgggctggg 1441 ccgtgatcac cgacgagtac aaggtgccca gcaagaaatt caaggtgctg ggcaacaccg 1501 accggcacag catcaagaag aacctgatcg gagccctgct gttcgacagc ggcgaaacag 1561 ccgaggccac ccggctgaag agaaccgcca gaagaagata caccagacgg aagaaccgga 1621 tctgctatct gcaagagatc ttcagcaacg agatggccaa ggtggacgac agcttcttcc 1681 acagactgga agagtccttc ctggtggaag aggataagaa gcacgagcgg caccccatct 1741 tcggcaacat cgtggacgag gtggcctacc acgagaagta ccccaccatc taccacctga 1801 gaaagaaact ggtggacagc accgacaagg ccgacctgcg gctgatctat ctggccctgg 1861 cccacatgat caagttccgg ggccacttcc tgatcgaggg cgacctgaac cccgacaaca 1921 gcgacgtgga caagctgttc atccagctgg tgcagaccta caaccagctg ttcgaggaaa 1981 accccatcaa cgccagcggc gtggacgcca aggccatcct gtctgccaga ctgagcaaga 2041 gcagacggct ggaaaatctg atcgcccagc tgcccggcga gaagaagaat ggcctgttcg 2101 gaaacctgat tgccctgagc ctgggcctga cccccaactt caagagcaac ttcgacctgg 2161 ccgaggatgc caaactgcag ctgagcaagg acacctacga cgacgacctg gacaacctgc 2221 tggcccagat cggcgaccag tacgccgacc tgtttctggc cgccaagaac ctgtccgacg 2281 ccatcctgct gagcgacatc ctgagagtga acaccgagat caccaaggcc cccctgagcg 2341 cctctatgat caagagatac gacgagcacc accaggacct gaccctgctg aaagctctcg 2401 tgcggcagca gctgcctgag aagtacaaag agattttctt cgaccagagc aagaacggct 2461 acgccggcta cattgacggc ggagccagcc aggaagagtt ctacaagttc atcaagccca 2521 tcctggaaaa gatggacggc accgaggaac tgctcgtgaa gctgaacaga gaggacctgc 2581 tgcggaagca gcggaccttc gacaacggca gcatccccca ccagatccac ctgggagagc 2641 tgcacgccat tctgcggcgg caggaagatt tttacccatt cctgaaggac aaccgggaaa 2701 agatcgagaa gatcctgacc ttccgcatcc cctactacgt gggccctctg gccaggggaa 2761 acagcagatt cgcctggatg accagaaaga gcgaggaaac catcaccccc tggaacttcg 2821 aggaagtggt ggacaagggc gcttccgccc agagcttcat cgagcggatg accaacttcg 2881 ataagaacct gcccaacgag aaggtgctgc ccaagcacag cctgctgtac gagtacttca 2941 ccgtgtataa cgagctgacc aaagtgaaat acgtgaccga gggaatgaga aagcccgcct 3001 tcctgagcgg cgagcagaaa aaggccatcg tggacctgct gttcaagacc aaccggaaag 3061 tgaccgtgaa gcagctgaaa gaggactact tcaagaaaat cgagtgcttc gactccgtgg 3121 aaatctccgg cgtggaagat cggttcaacg cctccctggg cacataccac gatctgctga 3181 aaattatcaa ggacaaggac ttcctggaca atgaggaaaa cgaggacatt ctggaagata 3241 tcgtgctgac cctgacactg tttgaggaca gagagatgat cgaggaacgg ctgaaaacct 3301 atgcccacct gttcgacgac aaagtgatga agcagctgaa gcggcggaga tacaccggct 3361 ggggcaggct gagccggaag ctgatcaacg gcatccggga caagcagtcc ggcaagacaa 3421 tcctggattt cctgaagtcc gacggcttcg ccaacagaaa cttcatgcag ctgatccacg 3481 acgacagcct gacctttaaa gaggacatcc agaaagccca ggtgtccggc cagggcgata 3541 gcctgcacga gcacattgcc aatctggccg gcagccccgc cattaagaag ggcatcctgc 3601 agacagtgaa ggtggtggac gagctcgtga aagtgatggg ccggcacaag cccgagaaca 3661 tcgtgatcga aatggccaga gagaaccaga ccacccagaa gggacagaag aacagccgcg 3721 agagaatgaa gcggatcgaa gagggcatca aagagctggg cagccagatc ctgaaagaac 3781 accccgtgga aaacacccag ctgcagaacg agaagctgta cctgtactac ctgcagaatg 3841 ggcgggatat gtacgtggac caggaactgg acatcaaccg gctgtccgac tacgatgtgg 3901 accatatcgt gcctcagagc tttctgaagg acgactccat cgacaacaag gtgctgacca 3961 gaagcgacaa gaaccggggc aagagcgaca acgtgccctc cgaagaggtc gtgaagaaga 4021 tgaagaacta ctggcggcag ctgctgaacg ccaagctgat tacccagaga aagttcgaca 4081 atctgaccaa ggccgagaga ggcggcctga gcgaactgga taaggccggc ttcatcaaga 4141 gacagctggt ggaaacccgg cagatcacaa agcacgtggc acagatcctg gactcccgga 4201 tgaacactaa gtacgacgag aatgacaagc tgatccggga agtgaaagtg atcaccctga 4261 agtccaagct ggtgtccgat ttccggaagg atttccagtt ttacaaagtg cgcgagatca 4321 acaactacca ccacgcccac gacgcctacc tgaacgccgt cgtgggaacc gccctgatca 4381 aaaagtaccc taagctggaa agcgagttcg tgtacggcga ctacaaggtg tacgacgtgc 4441 ggaagatgat cgccaagagc gagcaggaaa tcggcaaggc taccgccaag tacttcttct 4501 acagcaacat catgaacttt ttcaagaccg agattaccct ggccaacggc gagatccgga 4561 agcggcctct gatcgagaca aacggcgaaa ccggggagat cgtgtgggat aagggccggg 4621 attttgccac cgtgcggaaa gtgctgagca tgccccaagt gaatatcgtg aaaaagaccg 4681 aggtgcagac aggcggcttc agcaaagagt ctatcctgcc caagaggaac agcgataagc 4741 tgatcgccag aaagaaggac tgggacccta agaagtacgg cggcttcgac agccccaccg 4801 tggcctattc tgtgctggtg gtggccaaag tggaaaaggg caagtccaag aaactgaaga 4861 gtgtgaaaga gctgctgggg atcaccatca tggaaagaag cagcttcgag aagaatccca 4921 tcgactttct ggaagccaag ggctacaaag aagtgaaaaa ggacctgatc atcaagctgc 4981 ctaagtactc cctgttcgag ctggaaaacg gccggaagag aatgctggcc tctgccggcg 5041 aactgcagaa gggaaacgaa ctggccctgc cctccaaata tgtgaacttc ctgtacctgg 5101 ccagccacta tgagaagctg aagggctccc ccgaggataa tgagcagaaa cagctgtttg 5161 tggaacagca caagcactac ctggacgaga tcatcgagca gatcagcgag ttctccaaga 5221 gagtgatcct ggccgacgct aatctggaca aagtgctgtc cgcctacaac aagcaccggg 5281 ataagcccat cagagagcag gccgagaata tcatccacct gtttaccctg accaatctgg 5341 gagcccctgc cgccttcaag tactttgaca ccaccatcga ccggaagagg tacaccagca 5401 ccaaagaggt gctggacgcc accctgatcc accagagcat caccggcctg tacgagacac 5461 ggatcgacct gtctcagctg ggaggcgaca aaaggccggc ggccacgaaa aaggccggcc 5521 aggcaaaaaa gaaaaaggaa ttcggcagtg gagagggcag aggaagtctg ctaacatgcg 5581 gtgacgtcga ggagaatcct ggcccagtga gcaagggcga ggagctgttc accggggtgg 5641 tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg 5701 agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca 5761 agctgcccgt gccctggccc accctcgtga ccaccctgac ctacggcgtg cagtgcttca 5821 gccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct 5881 acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg 5941 tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg 6001 aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac aacgtctata 6061 tcatggccga caagcagaag aacggcatca aggtgaactt caagatccgc cacaacatcg 6121 aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc 6181 ccgtgctgct gcccgacaac cactacctga gcacccagtc cgccctgagc aaagacccca 6241 acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg 6301 gcatggacga gctgtacaag gaattctaac tagagctcgc tgatcagcct cgactgtgcc 6361 ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg 6421 tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag 6481 gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga 6541 gaatagcagg catgctgggg agcggccgca ggaaccccta gtgatggagt tggccactcc 6601 ctctctgcgc gctcgctcgc tcactgaggc cgggcgacca aaggtcgccc gacgcccggg 6661 ctttgcccgg gcggcctcag tgagcgagcg agcgcgcagc tgcctgcagg ggcgcctgat 6721 gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatacgtc aaagcaacca 6781 tagtacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg 6841 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc 6901 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga 6961 tttagtgctt tacggcacct cgaccccaaa aaacttgatt tgggtgatgg ttcacgtagt 7021 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat 7081 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcgggcta ttcttttgat 7141 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa 7201 tttaacgcga attttaacaa aatattaacg tttacaattt tatggtgcac tctcagtaca 7261 atctgctctg atgccgcata gttaagccag ccccgacacc cgccaacacc cgctgacgcg 7321 ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg 7381 agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc 7441 gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt 7501 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca 7561 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg 7621 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc 7681 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg 7741 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt 7801 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta 7861 ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat 7921 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga 7981 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca 8041 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact 8101 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc 8161 acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact 8221 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt 8281 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt 8341 ggaagccgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt 8401 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata 8461 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag 8521 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat 8581 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa 8641 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca 8701 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt 8761 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg 8821 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc 8881 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga 8941 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc 9001 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc 9061 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca 9121 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg 9181 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta 9241 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gcc //