LOCUS Exported 9292 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Encodes gRNA for 3' target of human RFXANK. ACCESSION . VERSION . KEYWORDS PX458_RFXANK_2 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 9292) AUTHORS Savic D, Partridge EC, Newberry KM, Smith SB, Meadows SK, Roberts BS, Mackiewicz M, Mendenhall EM, Myers RM TITLE CETCh-seq: CRISPR epitope tagging ChIP-seq of DNA-binding proteins. JOURNAL Genome Res. 2015 Sep 9. PUBMED 26355004 REFERENCE 2 (bases 1 to 9292) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..9292 /organism="synthetic DNA construct" /mol_type="other DNA" promoter 17..257 /label=U6 promoter /note="RNA polymerase III promoter for human U6 snRNA" primer_bind 17..37 /label=hU6-F /note="Human U6 promoter, forward primer" primer_bind 188..207 /label=LKO.1 5' /note="Human U6 promoter, forward primer" misc_RNA 285..360 /label=gRNA scaffold /note="guide RNA scaffold for the Streptococcus pyogenes CRISPR/Cas9 system" enhancer 457..742 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer; contains an 18-bp deletion relative to the standard CMV enhancer" promoter 744..1021 /label=chicken beta-actin promoter intron 1022..1250 /label=hybrid intron /note="hybrid between chicken beta-actin (CBA) and minute virus of mice (MMV) introns (Gray et al., 2011)" regulatory 1262..1271 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 1271..1336 /codon_start=1 /product="three tandem FLAG(R) epitope tags, followed by an enterokinase cleavage site" /label=3xFLAG /translation="DYKDHDGDYKDHDIDYKDDDDK" CDS 1343..1363 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" CDS 1388..5488 /codon_start=1 /product="Cas9 (Csn1) endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system" /label=Cas9 /note="generates RNA-guided double strand breaks in DNA" /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD ATLIHQSITGLYETRIDLSQLGGD" CDS 5489..5536 /codon_start=1 /product="bipartite nuclear localization signal from nucleoplasmin" /label=nucleoplasmin NLS /translation="KRPAATKKAGQAKKKK" CDS 5552..5605 /codon_start=1 /product="2A peptide from Thosea asigna virus capsid protein" /label=T2A /note="Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides." /translation="EGRGSLLTCGDVEENPGP" CDS 5606..6319 /codon_start=1 /product="enhanced GFP" /label=EGFP /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE FVTAAGITLGMDELYK" CDS 5606..6319 /codon_start=1 /product="the original enhanced GFP (Yang et al., 1996)" /label=EGFP /note="mammalian codon-optimized" /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE FVTAAGITLGMDELYK" primer_bind complement(5648..5669) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(5909..5928) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 6256..6277 /label=EGFP-C /note="EGFP, forward primer" primer_bind complement(6347..6364) /label=BGH-rev /note="Bovine growth hormone terminator, reverse primer. Also called BGH reverse" polyA_signal 6353..6560 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" repeat_region 6569..6709 /label=AAV2 ITR /note="inverted terminal repeat of adeno-associated virus serotype 2" repeat_region 6569..6698 /label=AAV2 ITR rep_origin 6784..7239 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(6871..6890) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 7081..7102 /label=F1ori-F /note="F1 origin, forward primer" primer_bind complement(7256..7275) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind 7375..7397 /label=pGEX 3' /note="pGEX vectors, reverse primer" primer_bind complement(7435..7453) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 7521..7625 /gene="bla" /label=AmpR promoter CDS 7626..8486 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(7844..7863) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 8657..9245 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 9146..9165 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 ttttgctcac atgtnngagg gcctatttcc catgattcct tcatatttgc atatacgata 61 caaggctgtt agagagataa ttggaattaa tttgactgta aacacaaaga tattagtaca 121 aaatacgtga cgtagaaagt aataatttct tgggtagttt gcagttttaa aattatgttt 181 taaaatggac tatcatatgc ttaccgtaac ttgaaagtat ttcgatttct tggctttata 241 tatcttgtgg aaaggacgaa acaccgcccg ctgaccctga gtgagtttta gagctagaaa 301 tagcaagtta aaataaggct agtccgttat caacttgaaa aagtggcacc gagtcggtgc 361 ttttttgttt tagagctaga aatagcaagt taaaataagg ctagtccgtt tttagcgcgt 421 gcgccaattc tgcagacaaa tggctctaga ggtacccgtt acataactta cggtaaatgg 481 cccgcctggc tgaccgccca acgacccccg cccattgacg tcaatagtaa cgccaatagg 541 gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca 601 tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc 661 ctggcattgt gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt 721 attagtcatc gctattacca tggtcgaggt gagccccacg ttctgcttca ctctccccat 781 ctcccccccc tccccacccc caattttgta tttatttatt ttttaattat tttgtgcagc 841 gatgggggcg gggggggggg gggggcgcgc gccaggcggg gcggggcggg gcgaggggcg 901 gggcggggcg aggcggagag gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt 961 tccttttatg gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc 1021 gggagtcgct gcgacgctgc cttcgccccg tgccccgctc cgccgccgcc tcgcgccgcc 1081 cgccccggct ctgactgacc gcgttactcc cacaggtgag cgggcgggac ggcccttctc 1141 ctccgggctg taattagctg agcaagaggt aagggtttaa gggatggttg gttggtgggg 1201 tattaatgtt taattacctg gagcacctgc ctgaaatcac tttttttcag gttggaccgg 1261 tgccaccatg gactataagg accacgacgg agactacaag gatcatgata ttgattacaa 1321 agacgatgac gataagatgg ccccaaagaa gaagcggaag gtcggtatcc acggagtccc 1381 agcagccgac aagaagtaca gcatcggcct ggacatcggc accaactctg tgggctgggc 1441 cgtgatcacc gacgagtaca aggtgcccag caagaaattc aaggtgctgg gcaacaccga 1501 ccggcacagc atcaagaaga acctgatcgg agccctgctg ttcgacagcg gcgaaacagc 1561 cgaggccacc cggctgaaga gaaccgccag aagaagatac accagacgga agaaccggat 1621 ctgctatctg caagagatct tcagcaacga gatggccaag gtggacgaca gcttcttcca 1681 cagactggaa gagtccttcc tggtggaaga ggataagaag cacgagcggc accccatctt 1741 cggcaacatc gtggacgagg tggcctacca cgagaagtac cccaccatct accacctgag 1801 aaagaaactg gtggacagca ccgacaaggc cgacctgcgg ctgatctatc tggccctggc 1861 ccacatgatc aagttccggg gccacttcct gatcgagggc gacctgaacc ccgacaacag 1921 cgacgtggac aagctgttca tccagctggt gcagacctac aaccagctgt tcgaggaaaa 1981 ccccatcaac gccagcggcg tggacgccaa ggccatcctg tctgccagac tgagcaagag 2041 cagacggctg gaaaatctga tcgcccagct gcccggcgag aagaagaatg gcctgttcgg 2101 aaacctgatt gccctgagcc tgggcctgac ccccaacttc aagagcaact tcgacctggc 2161 cgaggatgcc aaactgcagc tgagcaagga cacctacgac gacgacctgg acaacctgct 2221 ggcccagatc ggcgaccagt acgccgacct gtttctggcc gccaagaacc tgtccgacgc 2281 catcctgctg agcgacatcc tgagagtgaa caccgagatc accaaggccc ccctgagcgc 2341 ctctatgatc aagagatacg acgagcacca ccaggacctg accctgctga aagctctcgt 2401 gcggcagcag ctgcctgaga agtacaaaga gattttcttc gaccagagca agaacggcta 2461 cgccggctac attgacggcg gagccagcca ggaagagttc tacaagttca tcaagcccat 2521 cctggaaaag atggacggca ccgaggaact gctcgtgaag ctgaacagag aggacctgct 2581 gcggaagcag cggaccttcg acaacggcag catcccccac cagatccacc tgggagagct 2641 gcacgccatt ctgcggcggc aggaagattt ttacccattc ctgaaggaca accgggaaaa 2701 gatcgagaag atcctgacct tccgcatccc ctactacgtg ggccctctgg ccaggggaaa 2761 cagcagattc gcctggatga ccagaaagag cgaggaaacc atcaccccct ggaacttcga 2821 ggaagtggtg gacaagggcg cttccgccca gagcttcatc gagcggatga ccaacttcga 2881 taagaacctg cccaacgaga aggtgctgcc caagcacagc ctgctgtacg agtacttcac 2941 cgtgtataac gagctgacca aagtgaaata cgtgaccgag ggaatgagaa agcccgcctt 3001 cctgagcggc gagcagaaaa aggccatcgt ggacctgctg ttcaagacca accggaaagt 3061 gaccgtgaag cagctgaaag aggactactt caagaaaatc gagtgcttcg actccgtgga 3121 aatctccggc gtggaagatc ggttcaacgc ctccctgggc acataccacg atctgctgaa 3181 aattatcaag gacaaggact tcctggacaa tgaggaaaac gaggacattc tggaagatat 3241 cgtgctgacc ctgacactgt ttgaggacag agagatgatc gaggaacggc tgaaaaccta 3301 tgcccacctg ttcgacgaca aagtgatgaa gcagctgaag cggcggagat acaccggctg 3361 gggcaggctg agccggaagc tgatcaacgg catccgggac aagcagtccg gcaagacaat 3421 cctggatttc ctgaagtccg acggcttcgc caacagaaac ttcatgcagc tgatccacga 3481 cgacagcctg acctttaaag aggacatcca gaaagcccag gtgtccggcc agggcgatag 3541 cctgcacgag cacattgcca atctggccgg cagccccgcc attaagaagg gcatcctgca 3601 gacagtgaag gtggtggacg agctcgtgaa agtgatgggc cggcacaagc ccgagaacat 3661 cgtgatcgaa atggccagag agaaccagac cacccagaag ggacagaaga acagccgcga 3721 gagaatgaag cggatcgaag agggcatcaa agagctgggc agccagatcc tgaaagaaca 3781 ccccgtggaa aacacccagc tgcagaacga gaagctgtac ctgtactacc tgcagaatgg 3841 gcgggatatg tacgtggacc aggaactgga catcaaccgg ctgtccgact acgatgtgga 3901 ccatatcgtg cctcagagct ttctgaagga cgactccatc gacaacaagg tgctgaccag 3961 aagcgacaag aaccggggca agagcgacaa cgtgccctcc gaagaggtcg tgaagaagat 4021 gaagaactac tggcggcagc tgctgaacgc caagctgatt acccagagaa agttcgacaa 4081 tctgaccaag gccgagagag gcggcctgag cgaactggat aaggccggct tcatcaagag 4141 acagctggtg gaaacccggc agatcacaaa gcacgtggca cagatcctgg actcccggat 4201 gaacactaag tacgacgaga atgacaagct gatccgggaa gtgaaagtga tcaccctgaa 4261 gtccaagctg gtgtccgatt tccggaagga tttccagttt tacaaagtgc gcgagatcaa 4321 caactaccac cacgcccacg acgcctacct gaacgccgtc gtgggaaccg ccctgatcaa 4381 aaagtaccct aagctggaaa gcgagttcgt gtacggcgac tacaaggtgt acgacgtgcg 4441 gaagatgatc gccaagagcg agcaggaaat cggcaaggct accgccaagt acttcttcta 4501 cagcaacatc atgaactttt tcaagaccga gattaccctg gccaacggcg agatccggaa 4561 gcggcctctg atcgagacaa acggcgaaac cggggagatc gtgtgggata agggccggga 4621 ttttgccacc gtgcggaaag tgctgagcat gccccaagtg aatatcgtga aaaagaccga 4681 ggtgcagaca ggcggcttca gcaaagagtc tatcctgccc aagaggaaca gcgataagct 4741 gatcgccaga aagaaggact gggaccctaa gaagtacggc ggcttcgaca gccccaccgt 4801 ggcctattct gtgctggtgg tggccaaagt ggaaaagggc aagtccaaga aactgaagag 4861 tgtgaaagag ctgctgggga tcaccatcat ggaaagaagc agcttcgaga agaatcccat 4921 cgactttctg gaagccaagg gctacaaaga agtgaaaaag gacctgatca tcaagctgcc 4981 taagtactcc ctgttcgagc tggaaaacgg ccggaagaga atgctggcct ctgccggcga 5041 actgcagaag ggaaacgaac tggccctgcc ctccaaatat gtgaacttcc tgtacctggc 5101 cagccactat gagaagctga agggctcccc cgaggataat gagcagaaac agctgtttgt 5161 ggaacagcac aagcactacc tggacgagat catcgagcag atcagcgagt tctccaagag 5221 agtgatcctg gccgacgcta atctggacaa agtgctgtcc gcctacaaca agcaccggga 5281 taagcccatc agagagcagg ccgagaatat catccacctg tttaccctga ccaatctggg 5341 agcccctgcc gccttcaagt actttgacac caccatcgac cggaagaggt acaccagcac 5401 caaagaggtg ctggacgcca ccctgatcca ccagagcatc accggcctgt acgagacacg 5461 gatcgacctg tctcagctgg gaggcgacaa aaggccggcg gccacgaaaa aggccggcca 5521 ggcaaaaaag aaaaaggaat tcggcagtgg agagggcaga ggaagtctgc taacatgcgg 5581 tgacgtcgag gagaatcctg gcccagtgag caagggcgag gagctgttca ccggggtggt 5641 gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga 5701 gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa 5761 gctgcccgtg ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag 5821 ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta 5881 cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt 5941 gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga 6001 ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat 6061 catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga 6121 ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc 6181 cgtgctgctg cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa 6241 cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg 6301 catggacgag ctgtacaagg aattctaact agagctcgct gatcagcctc gactgtgcct 6361 tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt 6421 gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg 6481 tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagag 6541 aatagcaggc atgctgggga gcggccgcag gaacccctag tgatggagtt ggccactccc 6601 tctctgcgcg ctcgctcgct cactgaggcc gggcgaccaa aggtcgcccg acgcccgggc 6661 tttgcccggg cggcctcagt gagcgagcga gcgcgcagct gcctgcaggg gcgcctgatg 6721 cggtattttc tccttacgca tctgtgcggt atttcacacc gcatacgtca aagcaaccat 6781 agtacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga 6841 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg 6901 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat 6961 ttagtgcttt acggcacctc gaccccaaaa aacttgattt gggtgatggt tcacgtagtg 7021 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata 7081 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcgggctat tcttttgatt 7141 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat 7201 ttaacgcgaa ttttaacaaa atattaacgt ttacaatttt atggtgcact ctcagtacaa 7261 tctgctctga tgccgcatag ttaagccagc cccgacaccc gccaacaccc gctgacgcgc 7321 cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga 7381 gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgagacga aagggcctcg 7441 tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg 7501 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa 7561 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga 7621 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc 7681 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg 7741 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc 7801 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat 7861 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg 7921 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag 7981 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa 8041 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc 8101 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca 8161 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc 8221 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc 8281 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg 8341 gaagccgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta 8401 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag 8461 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga 8521 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc 8581 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa 8641 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa 8701 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc 8761 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt 8821 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc 8881 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac 8941 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca 9001 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg 9061 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag 9121 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt 9181 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat 9241 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg cc //