LOCUS Exported 3153 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Gateway Donor vector containing B2M , part of the Target Accelerator Plasmid Collection.. ACCESSION . VERSION . KEYWORDS pDONR223_B2M_p.A6V SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3153) AUTHORS Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn WC TITLE Systematic Functional Interrogation of Rare Cancer Variants Identifies Oncogenic Alleles. JOURNAL Cancer Discov. 2016 Jul;6(7):714-26. doi: 10.1158/2159-8290.CD-16-0160. Epub 2016 May 4. PUBMED 27147599 REFERENCE 2 (bases 1 to 3153) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..3153 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 43..65 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 57..74 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 58..74 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" protein_bind 90..189 /gene="mutant version of attL" /label=attL1 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" protein_bind complement(553..652) /gene="mutant version of attL" /label=attL2 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" primer_bind complement(669..688) /label=T7 /note="T7 promoter, forward primer" promoter complement(670..688) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(693..709) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(693..709) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" CDS 1140..1931 /codon_start=1 /gene="aadA" /product="aminoglycoside adenylyltransferase (Murphy, 1985)" /label=SmR /note="confers resistance to spectinomycin and streptomycin" /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK" rep_origin 2024..2612 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 2513..2532 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 2766..2783 /label=L4440 /note="L4440 vector, forward primer" terminator 2942..2969 /label=rrnB T2 terminator /note="transcription terminator T2 from the E. coli rrnB gene" terminator 3061..3147 /gene="Escherichia coli rrnB" /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" ORIGIN 1 ggcagttccc tactctcgcg ttaacgctag catggatgtt ttcccagtca cgacgttgta 61 aaacgacggc cagtcttaag ctcgggcccc aaataatgat tttattttga ctgatagtga 121 cctgttcgtt gcaacaaatt gatgagcaat gcttttttat aatgccaact ttgtacaaaa 181 aagttggcac catgtctcgc tccgtggtct tagctgtgct cgcgctactc tctctttctg 241 gcctggaggc tatccagcgt actccaaaga ttcaggttta ctcacgtcat ccagcagaga 301 atggaaagtc aaatttcctg aattgctatg tgtctgggtt tcatccatcc gacattgaag 361 ttgacttact gaagaatgga gagagaattg aaaaagtgga gcattcagac ttgtctttca 421 gcaaggactg gtctttctat ctcttgtact acactgaatt cacccccact gaaaaagatg 481 agtatgcctg ccgtgtgaac catgtgactt tgtcacagcc caagatagtt aagtgggatc 541 gagacatgtg attgccaact ttcttgtaca aagttggcat tataagaaag cattgcttat 601 caatttgttg caacgaacag gtcactatca gtcaaaataa aatcattatt tgccatccag 661 ctgatatccc ctatagtgag tcgtattaca tggtcatagc tgtttcctgg cagctctggc 721 ccgtgtctca aaatctctga tgttacattg cacaagataa aaatatatca tcatgcctcc 781 tctagaccag ccaggacaga aatgcctcga cttcgctgct acccaaggtt gccgggtgac 841 gcacaccgtg gaaacggatg aaggcacgaa cccagtggac ataagcctgt tcggttcgta 901 agctgtaatg caagtagcgt atgcgctcac gcaactggtc cagaaccttg accgaacgca 961 gcggtggtaa cggcgcagtg gcggttttca tggcttgtta tgactgtttt tttggggtac 1021 agtctatgcc tcgggcatcc aagcagcaag cgcgttacgc cgtgggtcga tgtttgatgt 1081 tatggagcag caacgatgtt acgcagcagg gcagtcgccc taaaacaaag ttaaacatta 1141 tgagggaagc ggtgatcgcc gaagtatcga ctcaactatc agaggtagtt ggcgtcatcg 1201 agcgccatct cgaaccgacg ttgctggccg tacatttgta cggctccgca gtggatggcg 1261 gcctgaagcc acacagtgat attgatttgc tggttacggt gaccgtaagg cttgatgaaa 1321 caacgcggcg agctttgatc aacgaccttt tggaaacttc ggcttcccct ggagagagcg 1381 agattctccg cgctgtagaa gtcaccattg ttgtgcacga cgacatcatt ccgtggcgtt 1441 atccagctaa gcgcgaactg caatttggag aatggcagcg caatgacatt cttgcaggta 1501 tcttcgagcc agccacgatc gacattgatc tggctatctt gctgacaaaa gcaagagaac 1561 atagcgttgc cttggtaggt ccagcggcgg aggaactctt tgatccggtt cctgaacagg 1621 atctatttga ggcgctaaat gaaaccttaa cgctatggaa ctcgccgccc gactgggctg 1681 gcgatgagcg aaatgtagtg cttacgttgt cccgcatttg gtacagcgca gtaaccggca 1741 aaatcgcgcc gaaggatgtc gctgccgact gggcaatgga gcgcctgccg gcccagtatc 1801 agcccgtcat acttgaagct agacaggctt atcttggaca agaagaagat cgcttggcct 1861 cgcgcgcaga tcagttggaa gaatttgtcc actacgtgaa aggcgagatc accaaggtag 1921 tcggcaaata accctcgagc cacccatgac caaaatccct taacgtgagt tacgcgtcgt 1981 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc 2041 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc 2101 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac 2161 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac 2221 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt 2281 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct 2341 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat 2401 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt 2461 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg 2521 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt 2581 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt 2641 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg 2701 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg 2761 agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc 2821 ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg 2881 gcagtgagcg caacgcaatt aatacgcgta ccgctagcca ggaagagttt gtagaaacgc 2941 aaaaaggcca tccgtcagga tggccttctg cttagtttga tgcctggcag tttatggcgg 3001 gcgtcctgcc cgccaccctc cgggccgttg cttcacaacg ttcaaatccg ctcccggcgg 3061 atttgtccta ctcaggagag cgttcaccga caaacaacag ataaaacgaa aggcccagtc 3121 ttccgactga gcctttcgtt ttatttgatg cct //