LOCUS Exported 3291 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Gateway Donor vector containing REG1A , part of the Target Accelerator Plasmid Collection.. ACCESSION . VERSION . KEYWORDS pDONR223_REG1A_p.S131I SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3291) AUTHORS Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn WC TITLE Systematic Functional Interrogation of Rare Cancer Variants Identifies Oncogenic Alleles. JOURNAL Cancer Discov. 2016 Jul;6(7):714-26. doi: 10.1158/2159-8290.CD-16-0160. Epub 2016 May 4. PUBMED 27147599 REFERENCE 2 (bases 1 to 3291) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..3291 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 43..65 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 57..74 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 58..74 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" protein_bind 90..189 /gene="mutant version of attL" /label=attL1 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" protein_bind complement(691..790) /gene="mutant version of attL" /label=attL2 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" primer_bind complement(807..826) /label=T7 /note="T7 promoter, forward primer" promoter complement(808..826) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(831..847) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(831..847) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" CDS 1278..2069 /codon_start=1 /gene="aadA" /product="aminoglycoside adenylyltransferase (Murphy, 1985)" /label=SmR /note="confers resistance to spectinomycin and streptomycin" /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK" rep_origin 2162..2750 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 2651..2670 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 2904..2921 /label=L4440 /note="L4440 vector, forward primer" terminator 3080..3107 /label=rrnB T2 terminator /note="transcription terminator T2 from the E. coli rrnB gene" terminator 3199..3285 /gene="Escherichia coli rrnB" /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" ORIGIN 1 ggcagttccc tactctcgcg ttaacgctag catggatgtt ttcccagtca cgacgttgta 61 aaacgacggc cagtcttaag ctcgggcccc aaataatgat tttattttga ctgatagtga 121 cctgttcgtt gcaacaaatt gatgagcaat gcttttttat aatgccaact ttgtacaaaa 181 aagttggcac catggctcag accagctcat acttcatgct gatctcctgc ctgatgtttc 241 tgtctcagag ccaaggccaa gaggcccaga cagagttgcc ccaggcccgg atcagctgcc 301 cagaaggcac caatgcctat cgctcctact gctactactt taatgaagac cgtgagacct 361 gggttgatgc agatctctat tgccagaaca tgaattcggg caacctggtg tctgtgctca 421 cccaggccga gggtgccttt gtggcctcac tgattaagga gagtggcact gatgacttca 481 atgtctggat tggcctccat gaccccaaaa agaaccgccg ctggcactgg agcagtgggt 541 ccctggtctc ctacaagtcc tggggcattg gagccccaag cattgttaat cctggctact 601 gtgtgagcct gacctcaagc acaggattcc agaaatggaa ggatgtgcct tgtgaagaca 661 agttctcctt tgtctgcaag ttcaaaaact atccaacttt cttgtacaaa gttggcatta 721 taagaaagca ttgcttatca atttgttgca acgaacaggt cactatcagt caaaataaaa 781 tcattatttg ccatccagct gatatcccct atagtgagtc gtattacatg gtcatagctg 841 tttcctggca gctctggccc gtgtctcaaa atctctgatg ttacattgca caagataaaa 901 atatatcatc atgcctcctc tagaccagcc aggacagaaa tgcctcgact tcgctgctac 961 ccaaggttgc cgggtgacgc acaccgtgga aacggatgaa ggcacgaacc cagtggacat 1021 aagcctgttc ggttcgtaag ctgtaatgca agtagcgtat gcgctcacgc aactggtcca 1081 gaaccttgac cgaacgcagc ggtggtaacg gcgcagtggc ggttttcatg gcttgttatg 1141 actgtttttt tggggtacag tctatgcctc gggcatccaa gcagcaagcg cgttacgccg 1201 tgggtcgatg tttgatgtta tggagcagca acgatgttac gcagcagggc agtcgcccta 1261 aaacaaagtt aaacattatg agggaagcgg tgatcgccga agtatcgact caactatcag 1321 aggtagttgg cgtcatcgag cgccatctcg aaccgacgtt gctggccgta catttgtacg 1381 gctccgcagt ggatggcggc ctgaagccac acagtgatat tgatttgctg gttacggtga 1441 ccgtaaggct tgatgaaaca acgcggcgag ctttgatcaa cgaccttttg gaaacttcgg 1501 cttcccctgg agagagcgag attctccgcg ctgtagaagt caccattgtt gtgcacgacg 1561 acatcattcc gtggcgttat ccagctaagc gcgaactgca atttggagaa tggcagcgca 1621 atgacattct tgcaggtatc ttcgagccag ccacgatcga cattgatctg gctatcttgc 1681 tgacaaaagc aagagaacat agcgttgcct tggtaggtcc agcggcggag gaactctttg 1741 atccggttcc tgaacaggat ctatttgagg cgctaaatga aaccttaacg ctatggaact 1801 cgccgcccga ctgggctggc gatgagcgaa atgtagtgct tacgttgtcc cgcatttggt 1861 acagcgcagt aaccggcaaa atcgcgccga aggatgtcgc tgccgactgg gcaatggagc 1921 gcctgccggc ccagtatcag cccgtcatac ttgaagctag acaggcttat cttggacaag 1981 aagaagatcg cttggcctcg cgcgcagatc agttggaaga atttgtccac tacgtgaaag 2041 gcgagatcac caaggtagtc ggcaaataac cctcgagcca cccatgacca aaatccctta 2101 acgtgagtta cgcgtcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt 2161 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac 2221 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct 2281 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact 2341 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg 2401 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata 2461 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga 2521 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag 2581 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg 2641 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac 2701 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca 2761 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg 2821 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc 2881 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa 2941 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt 3001 ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tacgcgtacc gctagccagg 3061 aagagtttgt agaaacgcaa aaaggccatc cgtcaggatg gccttctgct tagtttgatg 3121 cctggcagtt tatggcgggc gtcctgcccg ccaccctccg ggccgttgct tcacaacgtt 3181 caaatccgct cccggcggat ttgtcctact caggagagcg ttcaccgaca aacaacagat 3241 aaaacgaaag gcccagtctt ccgactgagc ctttcgtttt atttgatgcc t //