LOCUS Exported 3303 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Gateway Donor vector containing HRAS , part of the Target Accelerator Plasmid Collection.. ACCESSION . VERSION . KEYWORDS pDONR223_HRAS_p.Q61R SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3303) AUTHORS Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn WC TITLE Systematic Functional Interrogation of Rare Cancer Variants Identifies Oncogenic Alleles. JOURNAL Cancer Discov. 2016 Jul;6(7):714-26. doi: 10.1158/2159-8290.CD-16-0160. Epub 2016 May 4. PUBMED 27147599 REFERENCE 2 (bases 1 to 3303) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..3303 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 43..65 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 57..74 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 58..74 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" protein_bind 90..189 /gene="mutant version of attL" /label=attL1 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" protein_bind complement(703..802) /gene="mutant version of attL" /label=attL2 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" primer_bind complement(819..838) /label=T7 /note="T7 promoter, forward primer" promoter complement(820..838) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(843..859) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(843..859) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" CDS 1290..2081 /codon_start=1 /gene="aadA" /product="aminoglycoside adenylyltransferase (Murphy, 1985)" /label=SmR /note="confers resistance to spectinomycin and streptomycin" /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK" rep_origin 2174..2762 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 2663..2682 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 2916..2933 /label=L4440 /note="L4440 vector, forward primer" terminator 3092..3119 /label=rrnB T2 terminator /note="transcription terminator T2 from the E. coli rrnB gene" terminator 3211..3297 /gene="Escherichia coli rrnB" /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" ORIGIN 1 ggcagttccc tactctcgcg ttaacgctag catggatgtt ttcccagtca cgacgttgta 61 aaacgacggc cagtcttaag ctcgggcccc aaataatgat tttattttga ctgatagtga 121 cctgttcgtt gcaacaaatt gatgagcaat gcttttttat aatgacaact ttgtacaaaa 181 aagttggcac catgacggaa tataagctgg tggtggtggg cgccggcggt gtgggcaaga 241 gtgcgctgac catccagctg atccagaacc attttgtgga cgaatacgac cccactatag 301 aggattccta ccggaagcag gtggtcattg atggggagac gtgcctgttg gacatcctgg 361 ataccgccgg ccgggaggag tacagcgcca tgcgggacca gtacatgcgc accggggagg 421 gcttcctgtg tgtgtttgcc atcaacaaca ccaagtcttt tgaggacatc caccagtaca 481 gggagcagat caaacgggtg aaggactcgg atgacgtgcc catggtgctg gtggggaaca 541 agtgtgacct ggctgcacgc actgtggaat ctcggcaggc tcaggacctc gcccgaagct 601 acggcatccc ctacatcgag acctcggcca agacccggca gggcagccgc tctggctcta 661 gctccagctc cgggaccctc tgggaccccc cgggacccat gtgcccaact ttcttgtaca 721 aagttggcat tataagaaag cattgcttat caatttgttg caacgaacag gtcactatca 781 gtcaaaataa aatcattatt tgccatccag ctgatatccc ctatagtgag tcgtattaca 841 tggtcatagc tgtttcctgg cagctctggc ccgtgtctca aaatctctga tgttacattg 901 cacaagataa aaatatatca tcatgcctcc tctagaccag ccaggacaga aatgcctcga 961 cttcgctgct acccaaggtt gccgggtgac gcacaccgtg gaaacggatg aaggcacgaa 1021 cccagtggac ataagcctgt tcggttcgta agctgtaatg caagtagcgt atgcgctcac 1081 gcaactggtc cagaaccttg accgaacgca gcggtggtaa cggcgcagtg gcggttttca 1141 tggcttgtta tgactgtttt tttggggtac agtctatgcc tcgggcatcc aagcagcaag 1201 cgcgttacgc cgtgggtcga tgtttgatgt tatggagcag caacgatgtt acgcagcagg 1261 gcagtcgccc taaaacaaag ttaaacatta tgagggaagc ggtgatcgcc gaagtatcga 1321 ctcaactatc agaggtagtt ggcgtcatcg agcgccatct cgaaccgacg ttgctggccg 1381 tacatttgta cggctccgca gtggatggcg gcctgaagcc acacagtgat attgatttgc 1441 tggttacggt gaccgtaagg cttgatgaaa caacgcggcg agctttgatc aacgaccttt 1501 tggaaacttc ggcttcccct ggagagagcg agattctccg cgctgtagaa gtcaccattg 1561 ttgtgcacga cgacatcatt ccgtggcgtt atccagctaa gcgcgaactg caatttggag 1621 aatggcagcg caatgacatt cttgcaggta tcttcgagcc agccacgatc gacattgatc 1681 tggctatctt gctgacaaaa gcaagagaac atagcgttgc cttggtaggt ccagcggcgg 1741 aggaactctt tgatccggtt cctgaacagg atctatttga ggcgctaaat gaaaccttaa 1801 cgctatggaa ctcgccgccc gactgggctg gcgatgagcg aaatgtagtg cttacgttgt 1861 cccgcatttg gtacagcgca gtaaccggca aaatcgcgcc gaaggatgtc gctgccgact 1921 gggcaatgga gcgcctgccg gcccagtatc agcccgtcat acttgaagct agacaggctt 1981 atcttggaca agaagaagat cgcttggcct cgcgcgcaga tcagttggaa gaatttgtcc 2041 actacgtgaa aggcgagatc accaaggtag tcggcaaata accctcgagc cacccatgac 2101 caaaatccct taacgtgagt tacgcgtcgt tccactgagc gtcagacccc gtagaaaaga 2161 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa 2221 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga 2281 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt 2341 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt 2401 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat 2461 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct 2521 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca 2581 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag 2641 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc 2701 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga 2761 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca 2821 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag 2881 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg 2941 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct 3001 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatacgcgta 3061 ccgctagcca ggaagagttt gtagaaacgc aaaaaggcca tccgtcagga tggccttctg 3121 cttagtttga tgcctggcag tttatggcgg gcgtcctgcc cgccaccctc cgggccgttg 3181 cttcacaacg ttcaaatccg ctcccggcgg atttgtccta ctcaggagag cgttcaccga 3241 caaacaacag ataaaacgaa aggcccagtc ttccgactga gcctttcgtt ttatttgatg 3301 cct //