LOCUS Exported 3414 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Gateway Donor vector containing CD3E , part of the Target Accelerator Plasmid Collection.. ACCESSION . VERSION . KEYWORDS pDONR223_CD3E_p.E189Q SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3414) AUTHORS Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn WC TITLE Systematic Functional Interrogation of Rare Cancer Variants Identifies Oncogenic Alleles. JOURNAL Cancer Discov. 2016 Jul;6(7):714-26. doi: 10.1158/2159-8290.CD-16-0160. Epub 2016 May 4. PUBMED 27147599 REFERENCE 2 (bases 1 to 3414) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..3414 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 43..65 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 57..74 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 58..74 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" protein_bind 90..189 /gene="mutant version of attL" /label=attL1 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" protein_bind complement(814..913) /gene="mutant version of attL" /label=attL2 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" primer_bind complement(930..949) /label=T7 /note="T7 promoter, forward primer" promoter complement(931..949) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(954..970) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(954..970) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" CDS 1401..2192 /codon_start=1 /gene="aadA" /product="aminoglycoside adenylyltransferase (Murphy, 1985)" /label=SmR /note="confers resistance to spectinomycin and streptomycin" /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK" rep_origin 2285..2873 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 2774..2793 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 3027..3044 /label=L4440 /note="L4440 vector, forward primer" terminator 3203..3230 /label=rrnB T2 terminator /note="transcription terminator T2 from the E. coli rrnB gene" terminator 3322..3408 /gene="Escherichia coli rrnB" /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" ORIGIN 1 ggcagttccc tactctcgcg ttaacgctag catggatgtt ttcccagtca cgacgttgta 61 aaacgacggc cagtcttaag ctcgggcccc aaataatgat tttattttga ctgatagtga 121 cctgttcgtt gcaacaaatt gatgagcaat gcttttttat aatgccaact ttgtacaaaa 181 aagttggcat gcagtcgggc actcactgga gagttctggg cctctgcctc ttatcagttg 241 gtgtttgggg gcaagatggt aatgaagaaa tgggtggtat tacacagaca ccatataaag 301 tctccatctc tggaaccaca gtaatattga catgccctca gtatcctgga tctgaaatac 361 tatggcaaca caatgataaa aacataggcg gtgatgagga tgataaaaac ataggcagtg 421 atgaggatca cctgtcactg aaggaatttt cagaattgga gcaaagtggt tattatgtct 481 gctaccccag aggaagcaaa ccagaagatg cgaactttta tctctacctg agggcaagag 541 tgtgtgagaa ctgcatggag atggatgtga tgtcggtggc cacaattgtc atagtggaca 601 tctgcatcac tgggggcttg ctgctgctgg tttactactg gagcaagaat agaaaggcca 661 aggccaagcc tgtgacacga ggagcgggtg ctggcggcag gcaaagggga caaaacaagg 721 agaggccacc acctgttccc aacccagact atcagcccat ccggaaaggc cagcgggacc 781 tgtattctgg cctgaatcag agacgcatct gattgccaac tttcttgtac aaagttgtca 841 ttataagaaa gcattgctta tcaatttgtt gcaacgaaca ggtcactatc agtcaaaata 901 aaatcattat ttgccatcca gctgatatcc cctatagtga gtcgtattac atggtcatag 961 ctgtttcctg gcagctctgg cccgtgtctc aaaatctctg atgttacatt gcacaagata 1021 aaaatatatc atcatgcctc ctctagacca gccaggacag aaatgcctcg acttcgctgc 1081 tacccaaggt tgccgggtga cgcacaccgt ggaaacggat gaaggcacga acccagtgga 1141 cataagcctg ttcggttcgt aagctgtaat gcaagtagcg tatgcgctca cgcaactggt 1201 ccagaacctt gaccgaacgc agcggtggta acggcgcagt ggcggttttc atggcttgtt 1261 atgactgttt ttttggggta cagtctatgc ctcgggcatc caagcagcaa gcgcgttacg 1321 ccgtgggtcg atgtttgatg ttatggagca gcaacgatgt tacgcagcag ggcagtcgcc 1381 ctaaaacaaa gttaaacatt atgagggaag cggtgatcgc cgaagtatcg actcaactat 1441 cagaggtagt tggcgtcatc gagcgccatc tcgaaccgac gttgctggcc gtacatttgt 1501 acggctccgc agtggatggc ggcctgaagc cacacagtga tattgatttg ctggttacgg 1561 tgaccgtaag gcttgatgaa acaacgcggc gagctttgat caacgacctt ttggaaactt 1621 cggcttcccc tggagagagc gagattctcc gcgctgtaga agtcaccatt gttgtgcacg 1681 acgacatcat tccgtggcgt tatccagcta agcgcgaact gcaatttgga gaatggcagc 1741 gcaatgacat tcttgcaggt atcttcgagc cagccacgat cgacattgat ctggctatct 1801 tgctgacaaa agcaagagaa catagcgttg ccttggtagg tccagcggcg gaggaactct 1861 ttgatccggt tcctgaacag gatctatttg aggcgctaaa tgaaacctta acgctatgga 1921 actcgccgcc cgactgggct ggcgatgagc gaaatgtagt gcttacgttg tcccgcattt 1981 ggtacagcgc agtaaccggc aaaatcgcgc cgaaggatgt cgctgccgac tgggcaatgg 2041 agcgcctgcc ggcccagtat cagcccgtca tacttgaagc tagacaggct tatcttggac 2101 aagaagaaga tcgcttggcc tcgcgcgcag atcagttgga agaatttgtc cactacgtga 2161 aaggcgagat caccaaggta gtcggcaaat aaccctcgag ccacccatga ccaaaatccc 2221 ttaacgtgag ttacgcgtcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat 2281 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc 2341 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg 2401 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc 2461 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg 2521 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg 2581 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa 2641 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg 2701 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga 2761 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct 2821 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca 2881 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc 2941 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg 3001 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc 3061 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca 3121 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatacgcgt accgctagcc 3181 aggaagagtt tgtagaaacg caaaaaggcc atccgtcagg atggccttct gcttagtttg 3241 atgcctggca gtttatggcg ggcgtcctgc ccgccaccct ccgggccgtt gcttcacaac 3301 gttcaaatcc gctcccggcg gatttgtcct actcaggaga gcgttcaccg acaaacaaca 3361 gataaaacga aaggcccagt cttccgactg agcctttcgt tttatttgat gcct //