LOCUS Exported 3702 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Gateway Donor vector containing CDK4 , part of the Target Accelerator Plasmid Collection.. ACCESSION . VERSION . KEYWORDS pDONR223_CDK4_p.A23S SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3702) AUTHORS Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn WC TITLE Systematic Functional Interrogation of Rare Cancer Variants Identifies Oncogenic Alleles. JOURNAL Cancer Discov. 2016 Jul;6(7):714-26. doi: 10.1158/2159-8290.CD-16-0160. Epub 2016 May 4. PUBMED 27147599 REFERENCE 2 (bases 1 to 3702) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..3702 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 43..65 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 57..74 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 58..74 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" protein_bind 90..189 /gene="mutant version of attL" /label=attL1 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" protein_bind complement(1102..1201) /gene="mutant version of attL" /label=attL2 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" primer_bind complement(1218..1237) /label=T7 /note="T7 promoter, forward primer" promoter complement(1219..1237) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(1242..1258) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(1242..1258) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" CDS 1689..2480 /codon_start=1 /gene="aadA" /product="aminoglycoside adenylyltransferase (Murphy, 1985)" /label=SmR /note="confers resistance to spectinomycin and streptomycin" /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK" rep_origin 2573..3161 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 3062..3081 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 3315..3332 /label=L4440 /note="L4440 vector, forward primer" terminator 3491..3518 /label=rrnB T2 terminator /note="transcription terminator T2 from the E. coli rrnB gene" terminator 3610..3696 /gene="Escherichia coli rrnB" /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" ORIGIN 1 ggcagttccc tactctcgcg ttaacgctag catggatgtt ttcccagtca cgacgttgta 61 aaacgacggc cagtcttaag ctcgggcccc aaataatgat tttattttga ctgatagtga 121 cctgttcgtt gcaacaaatt gatgagcaat gcttttttat aatgacaact ttgtacaaaa 181 aagttggcac catggctacc tctcgatatg agccagtggc tgaaattggt gtcggtgcct 241 atgggacagt gtacaagtcc cgtgatcccc acagtggcca ctttgtggcc ctcaagagtg 301 tgagagtccc caatggagga ggaggtggag gaggccttcc catcagcaca gttcgtgagg 361 tggctttact gaggcgactg gaggcttttg agcatcccaa tgttgtccgg ctgatggacg 421 tctgtgccac atcccgaact gaccgggaga tcaaggtaac cctggtgttt gagcatgtag 481 accaggacct aaggacatat ctggacaagg cacccccacc aggcttgcca gccgaaacga 541 tcaaggatct gatgcgccag tttctaagag gcctagattt ccttcatgcc aattgcatcg 601 ttcaccgaga tctgaagcca gagaacattc tggtgacaag tggtggaaca gtcaagctgg 661 ctgactttgg cctggccaga atctacagct accagatggc acttacaccc gtggttgtta 721 cactctggta ccgagctccc gaagttcttc tgcagtccac atatgcaaca cctgtggaca 781 tgtggagtgt tggctgtatc tttgcagaga tgtttcgtcg aaagcctctc ttctgtggaa 841 actctgaagc cgaccagttg ggcaaaatct ttgacctgat tgggctgcct ccagaggatg 901 actggcctcg agatgtatcc ctgccccgtg gagcctttcc ccccagaggg ccccgcccag 961 tgcagtcggt ggtacctgag atggaggagt cgggagcaca gctgctgctg gaaatgctga 1021 cttttaaccc acacaagcga atctctgcct ttcgagctct gcagcactct tatctacata 1081 aggatgaagg taatccggag tgcccaactt tcttgtacaa agttggcatt ataagaaagc 1141 attgcttatc aatttgttgc aacgaacagg tcactatcag tcaaaataaa atcattattt 1201 gccatccagc tgatatcccc tatagtgagt cgtattacat ggtcatagct gtttcctggc 1261 agctctggcc cgtgtctcaa aatctctgat gttacattgc acaagataaa aatatatcat 1321 catgcctcct ctagaccagc caggacagaa atgcctcgac ttcgctgcta cccaaggttg 1381 ccgggtgacg cacaccgtgg aaacggatga aggcacgaac ccagtggaca taagcctgtt 1441 cggttcgtaa gctgtaatgc aagtagcgta tgcgctcacg caactggtcc agaaccttga 1501 ccgaacgcag cggtggtaac ggcgcagtgg cggttttcat ggcttgttat gactgttttt 1561 ttggggtaca gtctatgcct cgggcatcca agcagcaagc gcgttacgcc gtgggtcgat 1621 gtttgatgtt atggagcagc aacgatgtta cgcagcaggg cagtcgccct aaaacaaagt 1681 taaacattat gagggaagcg gtgatcgccg aagtatcgac tcaactatca gaggtagttg 1741 gcgtcatcga gcgccatctc gaaccgacgt tgctggccgt acatttgtac ggctccgcag 1801 tggatggcgg cctgaagcca cacagtgata ttgatttgct ggttacggtg accgtaaggc 1861 ttgatgaaac aacgcggcga gctttgatca acgacctttt ggaaacttcg gcttcccctg 1921 gagagagcga gattctccgc gctgtagaag tcaccattgt tgtgcacgac gacatcattc 1981 cgtggcgtta tccagctaag cgcgaactgc aatttggaga atggcagcgc aatgacattc 2041 ttgcaggtat cttcgagcca gccacgatcg acattgatct ggctatcttg ctgacaaaag 2101 caagagaaca tagcgttgcc ttggtaggtc cagcggcgga ggaactcttt gatccggttc 2161 ctgaacagga tctatttgag gcgctaaatg aaaccttaac gctatggaac tcgccgcccg 2221 actgggctgg cgatgagcga aatgtagtgc ttacgttgtc ccgcatttgg tacagcgcag 2281 taaccggcaa aatcgcgccg aaggatgtcg ctgccgactg ggcaatggag cgcctgccgg 2341 cccagtatca gcccgtcata cttgaagcta gacaggctta tcttggacaa gaagaagatc 2401 gcttggcctc gcgcgcagat cagttggaag aatttgtcca ctacgtgaaa ggcgagatca 2461 ccaaggtagt cggcaaataa ccctcgagcc acccatgacc aaaatccctt aacgtgagtt 2521 acgcgtcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc 2581 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg 2641 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag 2701 cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact 2761 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg 2821 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc 2881 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg 2941 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg 3001 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag 3061 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc 3121 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct 3181 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc 3241 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc 3301 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac 3361 cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact 3421 ggaaagcggg cagtgagcgc aacgcaatta atacgcgtac cgctagccag gaagagtttg 3481 tagaaacgca aaaaggccat ccgtcaggat ggccttctgc ttagtttgat gcctggcagt 3541 ttatggcggg cgtcctgccc gccaccctcc gggccgttgc ttcacaacgt tcaaatccgc 3601 tcccggcgga tttgtcctac tcaggagagc gttcaccgac aaacaacaga taaaacgaaa 3661 ggcccagtct tccgactgag cctttcgttt tatttgatgc ct //