LOCUS       Exported                 967 bp ds-DNA     linear   SYN 28-MAY-2021
DEFINITION  synthetic linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    FLAG-TRAF6-wt
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 967)
  AUTHORS   Zhong J, Kyriakis JM
  TITLE     Germinal center kinase is required for optimal Jun N-terminal kinase
            activation by Toll-like receptor agonists and is regulated by the 
            ubiquitin proteasome system and agonist-induced, TRAF6-dependent 
            stabilization.
  JOURNAL   Mol Cell Biol. 2004 Oct . 24(20):9165-75.
  PUBMED    15456887
REFERENCE   2  (bases 1 to 967)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 28, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..967
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     47..66
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        47..65
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             131..154
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             164..187
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 ctctctggct aactagagaa cccactgctt actggcttat cgaaattaat acgactcact
       61 atagggagac ccaagcttgg taccgagctc ggatccacta gtaacggccg ccagtgtgct
      121 ggaattgatg gactacaagg acgacgatga caaggaattg atggactaca aggacgacga
      181 tgacaaggaa ttcatgagtc tcttaaactg tgagaacagc tgcgggtcca gccagtcgtc
      241 cagtgactgc tgcgctgcca tggccgcctc ctgcagcgct gcagtgaaag atgacagcgt
      301 gagtggctct gccagcaccg ggaacctctc cagctccttc atggaggaga tccagggcta
      361 cgatgtggag tttgacccac ctctggagag caagtatgag tgtcccattt gcttgatggc
      421 tttacgggaa gcagtgcaaa caccatgtgg ccacaggttc tgcaaagcct gcatcatcaa
      481 atccataagg gatgcagggc acaagtgccc agttgacaat gaaatactgc tggaaaatca
      541 actgtttccc gacaattttg caaagcgaga gattctttcc ctgacggtaa agtgcccaaa
      601 taaaggctgt ttgcaaaaga tggaactgag acatctcgag gatcatcaag tacattgtga
      661 atttgctcta gtgaattgtc cccagtgcca acgtcctttc cagaagtgcc aggttaatac
      721 acacattatt gaggattgtc ccaggaggca ggtttcttgt gtaaactgtg ctgtgtccat
      781 ggcatatgaa gagaaagaga tccatgatca aagctgtcct ctggcaaata tcatctgtga
      841 atactgtggt acaatcctca tcagagaaca gatgcctaat cattatgatc tggactgccc
      901 aacagctcca atcccttgca cattcagtgt ttttggctgt catgaaaaga tgcagaggaa
      961 tcacttg
//
