LOCUS       Exported                7210 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Retroviral expression of mouse beta-catenin S33Y mutant.
ACCESSION   .
VERSION     .
KEYWORDS    pMXs-beta-catenin-S33Y
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7210)
  AUTHORS   Takahashi K, Yamanaka S
  TITLE     Induction of pluripotent stem cells from mouse embryonic and adult 
            fibroblast cultures by defined factors.
  JOURNAL   Cell. 2006 Aug 25. 126(4):663-76.
  PUBMED    16904174
REFERENCE   2  (bases 1 to 7210)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7210
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     complement(63..80)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(234..822)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(314..333)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(986..1846)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1609..1628
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1847..1951)
                     /gene="bla"
                     /label=AmpR promoter
     LTR             1981..2574
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    2637..2994
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             3053..3469
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     3399..3421
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     3437..3453
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     misc_feature    3479..3853
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     3791..3807
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     protein_bind    3904..3928
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3994..4029
                     /codon_start=1
                     /product="nanobody epitope tag corresponding to amino acids
                     16-27 of human beta-catenin (Traenkle et al., 2015)"
                     /label=BC2 tag
                     /translation="PDRKAAVSHWQQ"
     protein_bind    complement(6333..6357)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     LTR             6674..7143
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
ORIGIN
        1 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
       61 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
      121 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
      181 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
      241 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
      301 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
      361 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
      421 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
      481 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
      541 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
      601 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
      661 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
      721 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
      781 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
      841 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
      901 ttatcaaaaa ggatcttcac ctagatcctt ttgcggccgg ccgcaaatca atctaaagta
      961 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
     1021 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
     1081 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
     1141 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
     1201 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
     1261 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
     1321 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
     1381 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
     1441 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
     1501 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
     1561 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
     1621 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
     1681 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
     1741 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
     1801 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
     1861 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
     1921 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgcat
     1981 aatgaaagac cccacctgta ggtttggcaa gctagcttaa gtaacgccat tttgcaaggc
     2041 atggaaaaat acataactga gaatagaaaa gttcagatca aggtcaggaa cagatggaac
     2101 agctgaatat gggccaaaca ggatatctgt ggtaagcagt tcctgccccg gctcagggcc
     2161 aagaacagat ggaacagctg aatatgggcc aaacaggata tctgtggtaa gcagttcctg
     2221 ccccggctca gggccaagaa cagatggtcc ccagatgcgg tccagccctc agcagtttct
     2281 agagaaccat cagatgtttc cagggtgccc caaggacctg aaatgaccct gtgccttatt
     2341 tgaactaacc aatcagttcg cttctcgctt ctgttcgcgc gcttctgctc cccgagctca
     2401 ataaaagagc ccacaacccc tcactcggcg cgccagtcct ccgattgact gagtcgcccg
     2461 ggtacccgtg tatccaataa accctcttgc agttgcatcc gacttgtggt ctcgctgttc
     2521 cttgggaggg tctcctctga gtgattgact acccgtcagc gggggtcttt catttggggg
     2581 ctcgtccggg atcgggagac ccctgcccag ggaccaccga cccaccaccg ggaggtaagc
     2641 tggccagcaa cttatctgtg tctgtccgat tgtctagtgt ctatgactga ttttatgcgc
     2701 ctgcgtcggt actagttagc taactagctc tgtatctggc ggacccgtgg tggaactgac
     2761 gagttcggaa cacccggccg caaccctggg agacgtccca gggacttcgg gggccgtttt
     2821 tgtggcccga cctgagtcca aaaatcccga tcgttttgga ctctttggtg caccccccta
     2881 ataggaggga tatgtggttc tggtaggaga cgagaaccta aaacagttcc cgcctccgtc
     2941 tgaatttttg ctttcggttt gggaccgaag ccgcgccgcg cgtcttgtct gctgcagcat
     3001 cgttctgtgt tgtctctgtc tgactgtgtt tctgtatttg tctgaaaatt agggccagac
     3061 tgttaccact cccttaagtt tgaccttagg tcactggaaa gatgtcgagc ggatcgctca
     3121 caaccagtcg gtagatgtca agaagagacg ttgggttacc ttctgctctg cagaatggcc
     3181 aacctttaac gtcggatggc cgcgagacgg cacctttaac cgagacctca tcacccaggt
     3241 taagatcaag gtcttttcac ctggcccgca tggacaccca gaccaggtcc cctacatcgt
     3301 gacctgggaa gccttggctt ttgacccccc tccctgggtc aagccctttg tacaccctaa
     3361 gcctccgcct cctcttcctc catccgcccc gtctctcccc cttgaacctc ctcgttcgac
     3421 cccgcctcga tcctcccttt atccagccct cactccttct ctaggcgccc ccatatggcc
     3481 atatgagatc ttatatgggg cacccccgcc ccttgtaaac ttccctgacc ctgacatgac
     3541 aagagttact aacagcccct ctctccaagc tcacttacag gctctctact tagtccagca
     3601 cgaagtctgg agacctctgg cggcagccta ccaagaacaa ctggaccgac cggtggtacc
     3661 tcacccttac cgagtcggcg acacagtgtg ggtccgccga caccagacta agaacctaga
     3721 acctcgctgg aaaggacctt acacagtcct gctgaccacc cccaccgccc tcaaagtaga
     3781 cggcatcgca gcttggatac acgccgccca cgtgaaggct gccgaccccg ggggtggacc
     3841 atcctctaga ctgccggatc tagctagtta attaaggatc ccagtgtggt ggtacgggaa
     3901 atcacaagtt tgtacaaaaa agcaggctcc gcggccgccc ccttcaccat ggctactcaa
     3961 gctgacctga tggagttgga catggccatg gagccggaca gaaaagctgc tgtcagccac
     4021 tggcagcagc agtcttactt agattatgga atccattctg gtgccaccac cacagctcct
     4081 tccctgagtg gcaagggcaa ccctgaggaa gaagatgttg acacctccca agtcctttat
     4141 gaatgggagc aaggcttttc ccagtccttc acgcaagagc aagtagctga tattgacggg
     4201 cagtatgcaa tgactagggc tcagagggtc cgagctgcca tgttccctga gacgctagat
     4261 gagggcatgc agatcccatc cacgcagttt gacgctgctc atcccactaa tgtccagcgc
     4321 ttggctgaac catcacagat gttgaaacat gcagttgtca atttgattaa ctatcaggat
     4381 gacgcggaac ttgccacacg tgcaattcct gagctgacaa aactgctaaa cgatgaggac
     4441 caggtggtag ttaataaagc tgctgttatg gtccatcagc tttccaaaaa ggaagcttcc
     4501 agacatgcca tcatgcgctc ccctcagatg gtgtctgcca ttgtacgcac catgcagaat
     4561 acaaatgatg tagagacagc tcgttgtact gctgggactc tgcacaacct ttctcaccac
     4621 cgcgagggct tgctggccat ctttaagtct ggtggcatcc cagcgctggt gaaaatgctt
     4681 gggtcaccag tggattctgt actgttctac gccatcacga cactgcataa tctcctgctc
     4741 catcaggaag gagctaaaat ggcagtgcgc ctagctggtg gactgcagaa aatggttgct
     4801 ttgctcaaca aaacaaacgt gaaattcttg gctattacaa cagactgcct tcagatctta
     4861 gcttatggca atcaagagag caagctcatc attctggcca gtggtggacc ccaagcctta
     4921 gtaaacataa tgaggaccta cacttatgag aagcttctgt ggaccacaag cagagtgctg
     4981 aaggtgctgt ctgtctgctc tagcaacaag ccggccattg tagaagctgg tgggatgcag
     5041 gcactggggc ttcatctgac agacccaagt cagcgacttg ttcaaaactg tctttggact
     5101 ctcagaaacc cttcagatgc agcgactaag caggaaggga tggaaggcct ccttgggact
     5161 ctagtgcagc ttctgggttc cgatgatata aatgtggtca cctgtgcagc tggaattctc
     5221 tctaacctca cttgcaataa ttacaaaaac aagatgatgg tgtgccaagt gggtggcata
     5281 gaggctcttg tacgcaccgt ccttcgtgct ggtgacaggg aagacatcac tgagcctgcc
     5341 atctgtgctc ttcgtcatct gaccagccgg catcaggaag ccgagatggc ccagaatgcc
     5401 gttcgccttc attatggact gcctgttgtg gttaaactcc tgcacccacc atcccactgg
     5461 cctctgataa aggcaactgt tggattgatt cgaaaccttg ccctttgccc agcaaatcat
     5521 gcgcctttgc gggaacaggg tgctattcca cgactagttc agctgcttgt acgagcacat
     5581 caggacaccc aacggcgcac ctccatgggt ggaacgcagc agcagtttgt ggagggcgtg
     5641 cgcatggagg agatagtaga agggtgtact ggagctctcc acatccttgc tcgggacgtt
     5701 cacaaccgga ttgtaatccg aggactcaat accattccat tgtttgtgca gttgctttat
     5761 tctcccattg aaaatatcca aagagtagct gcaggggtcc tctgtgaact tgctcaggac
     5821 aaggaggctg cagaggccat tgaagctgag ggagccacag ctcccctgac agagttactc
     5881 cactccagga atgaaggcgt ggcaacatac gcagctgctg tcctattccg aatgtctgag
     5941 gacaagccac aggattacaa gaagcggctt tcagtcgagc tgaccagttc cctcttcagg
     6001 acagagccaa tggcttggaa tgagactgca gatcttggac tggacattgg tgcccaggga
     6061 gaagcccttg gatatcgcca ggatgatccc agctaccgtt cttttcactc tggtggatac
     6121 ggccaggatg ccttggggat ggaccctatg atggagcatg agatgggtgg ccaccaccct
     6181 ggtgctgact atccagttga tgggctgcct gatctgggac acgcccagga cctcatggat
     6241 gggctgcccc caggtgatag caatcagctg gcctggtttg atactgacct gtaaatcgtc
     6301 ctttaggtaa gaaagcaagg gtgggcgcgc cgacccagct ttcttgtaca aagtggtgat
     6361 attccagctg agcgccggtc gctaccatta ccagttggtc tggtgtcaaa aataataata
     6421 accgggcagg ccatgtctgc ccgtatttcg cgtaaggaaa tccattatgt actatttaaa
     6481 ctcgagcggc cgccagcaca gtggtcgacg ataaaataaa agattttatt tagtctccag
     6541 aaaaaggggg gaatgaaaga ccccacctgt aggtttggca agctagctta agtaacgcca
     6601 ttttgcaagg catggaaaaa tacataactg agaatagaga agttcagatc aaggtcagga
     6661 acagatggaa cagctgaata tgggccaaac aggatatctg tggtaagcag ttcctgcccc
     6721 ggctcagggc caagaacaga tggaacagct gaatatgggc caaacaggat atctgtggta
     6781 agcagttcct gccccggctc agggccaaga acagatggtc cccagatgcg gtccagccct
     6841 cagcagtttc tagagaacca tcagatgttt ccagggtgcc ccaaggacct gaaatgaccc
     6901 tgtgccttat ttgaactaac caatcagttc gcttctcgct tctgttcgcg cgcttctgct
     6961 ccccgagctc aataaaagag cccacaaccc ctcactcggg gcgccagtcc tccgattgac
     7021 tgagtcgccc gggtacccgt gtatccaata aaccctcttg cagttgcatc cgacttgtgg
     7081 tctcgctgtt ccttgggagg gtctcctctg agtgattgac tacccgtcag cgggggtctt
     7141 tcacatgcag catgtatcaa aattaatttg gttttttttc ttaagtattt acattaaatg
     7201 gccatagttg
//
