LOCUS Exported 8611 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Expression of UTX in mammalian cells. ACCESSION . VERSION . KEYWORDS pCS2-UTX-F SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 8611) AUTHORS Hong S, Cho YW, Yu LR, Yu H, Veenstra TD, Ge K TITLE Identification of JmjC domain-containing UTX and JMJD3 as histone H3 lysine 27 demethylases. JOURNAL Proc Natl Acad Sci U S A. 2007 Nov 20. 104(47):18439-44. PUBMED 18003914 REFERENCE 2 (bases 1 to 8611) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..8611 /organism="synthetic DNA construct" /mol_type="other DNA" protein_bind 55..76 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 91..121 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 129..145 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 134..156 /label=M13/pUC Reverse /note="In lacZ gene" primer_bind 153..169 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 153..169 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind 188..208 /label=T3 /note="T3 promoter, forward primer" promoter 190..208 /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" polyA_signal 321..455 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(358..377) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 412..431 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" promoter 460..477 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" CDS complement(489..512) /codon_start=1 /product="FLAG(R) epitope tag, followed by an enterokinase cleavage site" /label=FLAG /translation="DYKDDDDK" CDS complement(4734..4763) /codon_start=1 /product="Myc (human c-Myc proto-oncogene) epitope tag" /label=Myc /translation="EQKLISEEDL" CDS complement(4797..4826) /codon_start=1 /product="Myc (human c-Myc proto-oncogene) epitope tag" /label=Myc /translation="EQKLISEEDL" CDS complement(4836..4865) /codon_start=1 /product="Myc (human c-Myc proto-oncogene) epitope tag" /label=Myc /translation="EQKLISEEDL" CDS complement(4875..4904) /codon_start=1 /product="Myc (human c-Myc proto-oncogene) epitope tag" /label=Myc /translation="EQKLISEEDL" CDS complement(4914..4943) /codon_start=1 /product="Myc (human c-Myc proto-oncogene) epitope tag" /label=Myc /translation="EQKLISEEDL" CDS complement(4953..4982) /codon_start=1 /product="Myc (human c-Myc proto-oncogene) epitope tag" /label=Myc /translation="EQKLISEEDL" CDS complement(5001..5021) /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" regulatory 5024..5033 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" promoter complement(5065..5083) /label=SP6 promoter /note="promoter for bacteriophage SP6 RNA polymerase" primer_bind complement(5066..5083) /label=SP6 /note="SP6 promoter, forward primer" promoter complement(5118..6095) /label=CMV IE94 promoter /note="enhancer/promoter region of simian cytomegalovirus major immediate early transcription unit IE94" rep_origin 6173..6628 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(6260..6279) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 6470..6491 /label=F1ori-F /note="F1 origin, forward primer" promoter 6654..6758 /gene="bla" /label=AmpR promoter CDS 6759..7619 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(6977..6996) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 7790..8378 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 8279..8298 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 8532..8549 /label=L4440 /note="L4440 vector, forward primer" ORIGIN 1 cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt 61 gagttagctc actcattagg caccccaggc tttacacttt atgctcccgg ctcgtatgtt 121 gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacgc 181 caagcgcgca attaaccctc actaaaggga acaaaagctg ggtaccgggc ccaatgcatt 241 ggcgccgcgg ccgcgaatta aaaaacctcc cacacctccc cctgaacctg aaacataaaa 301 tgaatgcaat tgttgttgtt aacttgttta ttgcagctta taatggttac aaataaagca 361 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt 421 ccaaactcat caatgtatct tatcatgtct ggatctacgt aatacgactc actatagttc 481 tagaatcact tgtcatcgtc gtccttgtag tcagatgagg cggatggtaa tggaggagct 541 aatgtaaatt ggtcatagac ttgcatcagg tcctccattt tgtactgttc tagcaccaca 601 aagttttcca agtttccgct tgtttttcgt gcacaatctt ggcaatgtac tatgtaggtc 661 tttcgtgaat tactctcatt agtgacaaaa agcagatcaa aaacctccac ttcacaaatg 721 ctacagtaat gagctggttc tttttttgtc cgcccatgcc atataatctc ttttcctgca 781 gcaatgagag cttccctcaa tgtctgacat tgcttcagag ttcttagaag acaatactta 841 atcatttcaa aaagctttgg atctgagacc ttgatatttc gtgccatatt ccaggaaaga 901 tgaaccatgg gtactattga cttcacactt tgcaatttgt tccattcgta ccgttccact 961 gccaatttat actggcaggc tgtaagtgga ccaacattcc aagcaatgtt gttgcaccag 1021 ccaatagcct gaacccagtg aacagtgcct gcatttatcc agaccaaatc tccaggtcgc 1081 tgaataaacc tatacactgg aacatttgct tcataaagat cttcaagatt gggccaccaa 1141 gaacccatta ggaaattcaa attatttttt tcacagaagt cattcagaac accccagtaa 1201 ccttcaggaa caacaaacca ttcacagtca cctgggccaa tatttatgtt aactgaacag 1261 aagttgttat tttcctgatg acctggtgtt ctgctccctg gaactttcat gtatagttga 1321 actgtgttca tgcccaatat ggtatgacca acatggctta gaagatttcc tgctgatacg 1381 acacgcacaa aagcaggaag tttagtcagc tcatgtagct gcaacttcca ctttttgtca 1441 tcagataggt caatattggt cccaaacttt atggttttaa agggtccttt cctcctcctc 1501 ccagaattat ctgacgatgt agattcacta tctgagtggt ctttatgatg acttcttttt 1561 tcattttctt ctctcaatga ttcctggaat gaggaggcct ggtactgtgc atatttagca 1621 attgtagtat gagatctatt actttcacaa tgccagattt tctttgttcc agtggggtcc 1681 cagttttcat ctgctggctg caacaactgt gtcctcactt ctaccatatg ttcattgtta 1741 gcttccacca aagttttagt agagaaaagt cccaggtcta acttaagagc tccagcaagg 1801 ccacgtatta ctgtaacagg gttgttcgga tttgtacaaa attgatgtaa tggaggaaag 1861 aaagcatcac gtttattttc caagtaaata ctaggtgtag gtggattcaa cttgtccttt 1921 ggcaagggag ggtatggtga agatggtggt cttggaggtg gacatttatc caacaaaatg 1981 ctactgttag ataagccatt tttacctaga ttcctgcatg ccttcagaac ttctgctgag 2041 ctggggtata tggacacaga cattgatggt atgatctgtg gactaggttt gtggttaacc 2101 agaagcagat ctgttttcat ggggctctga gattcttcca tcccttctcc attaattgtg 2161 tgtagcccac tgtgagggct gttaaggctg gtaacactgt ttgtggttgt ctgctcagtg 2221 gattttggag aaggtgttgc tgttgaaatg gctgaagatg gtgaagaggc aacagagtta 2281 tcagtctttg tatgaacagc tgggtggatg ttattgactt tgtcacaggt tccagtaccc 2341 acattgttat tggcttttcc catcaacaag gcagagagct gaggattgtc tgaacttagt 2401 aaacctggtg acttagaatc tccatggcta ggactgcaaa cagcatctgc cgtcatctga 2461 tggacatgat taggaagtcc ctcgacactg gcagtgctgt taggtgtctc tccagtgtgc 2521 ctgcttgttt caggcacctt caatatgttt cctgaaggct tgctctcttt ggttaaggta 2581 atgccttgtt gtccacctga ggtagcagtg tgagaggaga ggtgattgag agcagccccc 2641 tgtgttactg aattgctagg cagggtggga tgtccgttct gattctgaat accagagcca 2701 gctgcctgga gatgctggga aggcccagtg gaagagagag gtcgttcacc attaggacct 2761 gccgaatgtg aactctgacc tttgtgaagc ccctgagtgg agttagatag ttggtttttc 2821 cacggctcct ctgcgctgct ggtcaggttt gtgcggttat gaggtagagt gagtgcgttt 2881 cgctgcaggt aaggcgcgtc tccattactt ccacttcctg ttatatgatt attgcctatc 2941 aaaatagtgt ctgtacttcc cagcgttctt gatgtgctac agggaacatg gcctgcagaa 3001 aagggtccat tggccaaagg ctgcccaggg caggcaggac ggactccagg ctgagagacg 3061 ctaggcactc tggtcagagc aagctgtggc tgctggccag agactgagtt tgtaggcagt 3121 gatgagtcag ctgttggccc attaggaatt ccagtagatc gtacctgtgc aactcctgtt 3181 ggtctcattt ggtgttgttg cattaaggca aactgacttt ccagctgttc cagcatcagt 3241 ttctgtgctg gatttaaatt atttctattt gcgcggagct gttccaaatg ctgtaatttc 3301 tgtggtgtca aacaccatga atgagcttgt tgctgtactg gaggatgtga cacagcatgt 3361 ccaccactcc aattgtcaga agtattctgc tgtgctgtgt tcatggcacc ctgcctggag 3421 gtaagctctg cgggaattgg taggctccac gcctcctcaa tactaggaag taatttagtt 3481 ttattctgta gactaccttg tggaaggtta cacaactgag cctgtaaata cttaattcgt 3541 gctgcaagtg cagaggtatt actacaactt ttgcttctag ttgcatttaa gtagcattta 3601 atggcatcct gaggctggtt gcaggattca tagagagtgc ctaggtccat ccaggctgca 3661 gcatggccat ggtccaattg tacagcacaa atataggcct gtaaagcatc catgggctga 3721 ttttgctgct gatatagcac acctattgaa caccatgtat ctgcacttgc ttctgattta 3781 tcaatagact gcctgtaaga tataaaggca tcctgaactt tcccaatact tgaatagcac 3841 cttccgagga aataccagga ctggccagaa ttaggatctg cttccaagga cttttggaga 3901 tactgaatag catagctttc cttggtggct ttatctccca ggaggtctac agtgtgatgc 3961 atccaaccta actgttgtaa gacagttgct tttacttgtg cagaaagatt ctctgtctgc 4021 aaaagttgtt cataagcttc ttttgcagaa tgatattccc tctgggtttc atataagtgg 4081 gcaatgtgaa attgaatttc agcattggac aaagtgcagg gattacagtc aaccaaagct 4141 aactgaaaat gctttaaact agactcatag tctgtgttca ctttgaacat aagcccaagt 4201 cgtaaatgaa tttccttggc tcgacaaaag ctgggatcaa cataaagcac ctcctgaaat 4261 gctttaattg cccactgaaa tgcattataa tggaagtaga ccaaaccaag accatataaa 4321 aaggcagcat tcttccagta gtcagactgt aaactgtagt acctctggta tgcagataat 4381 gcttttggat aatcttccaa taagaggttg aagtgaccta attgacaaaa gaaatcagac 4441 tccacttttc cttcagcttt taagattaga gattcatagc agcgaacagc cttgcccagt 4501 agggccttcg tcctggcgcc atcttcatga aatctcacga acccgaagag gcggctgtcc 4561 agtccgccga gcgcctccct ctcctcggct gtcaggctgg gggacgcctc ctcgctctcg 4621 ccgctcgctt ttcccgccgc cattttcttt tcctcatcac cgaaagcggc ggcggcagcg 4681 gcggcggtag cgagcgacac tccgcaggat ttcatctcga gaggccttga attcaagtcc 4741 tcttcagaaa tgagcttttg ctccatggtg aggtcgccca agctctccat ttcattcaag 4801 tcctcttcag aaatgagctt ttgctccatt tcattcaagt cctcttcaga aatgagcttt 4861 tgctccattt cattcaagtc ctcttcagaa atgagctttt gctccatttc attcaagtcc 4921 tcttcagaaa tgagcttttg ctccatttca ttcaagtcct cttcagaaat gagcttttgc 4981 tccatagctt tcgatcgatt taccttacgc ttcttctttg gagccatggc ggcggatcct 5041 gcaaaaagaa caagtagctt gtattctata gtgtcaccta aatcaagctt gctccgacgt 5101 ccccaggcag aatggcggtt ccctaaacga gcattgctta tatagacctc ccattaggca 5161 cgcctaccgc ccatttacgt caatggaacg cccatttgcg tcattgcccc tccccattga 5221 cgtcaatggg gatgtacttg gcagccatcg cgggccattt accgccattg acgtcaatgg 5281 gagtactgcc aatgtaccct ggcgtacttc caatagtaat gtacttgcca agttactatt 5341 aatagatatt gatgtactgc caagtgggcc atttaccgtc attgacgtca atagggggcg 5401 tgagaacgga tatgaatggg caatgagcca tcccattgac gtcaatggtg ggtggtccta 5461 ttgacgtcaa tgggcattga gccaggcggg ccatttaccg taattgacgt caatggggga 5521 ggcgccatat acgtcaatag gaccgcccat atgacgtcaa taggaaagac catatataga 5581 gaccattgac gtcaatgggg gagtggctat gggcggtatt aggaagcccc atatatggta 5641 tatgggaccg cccattggga ggggctatct acgtcaatag gaaaacccat atatggaata 5701 ctatatggca tagggccaat acatagtatt gaacctggcc aatagccata ttggcatagg 5761 gccatattgg atattgccta tatattgatc ctggcatata gccaatatgg ccgccattat 5821 tggcaccatg ccaattcaat atggcggact tggcacaggg ccaagacccc tcctcaaact 5881 tggcaccgtg ccaagtagac ccctccccag ttggcacagt gccaggtccg ccatattgaa 5941 ttggcatggt gccaagtccg ccatattgaa ttggctgggg tccagatcca ccatattgaa 6001 ttggcacgag tccatatacg ccatattgaa ttggcacagg tccagatcca ccatattgaa 6061 ttggcatgag tccatatacg ccatattgaa ttggcggtcg actggcgtaa tagcgaagag 6121 gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg ggacgcgccc 6181 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt 6241 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc 6301 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta 6361 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc 6421 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg 6481 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt 6541 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat 6601 tttaacaaaa tattaacgct tacaatttag gtggcacttt tcggggaaat gtgcgcggaa 6661 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac 6721 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg 6781 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc 6841 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg 6901 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga 6961 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc 7021 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag 7081 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga 7141 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg 7201 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga 7261 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt 7321 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact 7381 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt 7441 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg 7501 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta 7561 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac 7621 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta 7681 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt 7741 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt 7801 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt 7861 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc 7921 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg 7981 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg 8041 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt 8101 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac 8161 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg 8221 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg 8281 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat 8341 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt 8401 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg 8461 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa 8521 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc 8581 ctctccccgc gcgttggccg attcattaat g //