LOCUS       Exported                4005 bp ds-DNA     circular SYN 13-MAY-2021
DEFINITION  Gateway Donor vector containing PTEN, part of the Target Accelerator
            Plasmid Collection..
ACCESSION   .
VERSION     .
KEYWORDS    pDONR223_PTEN_p.F90S
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4005)
  AUTHORS   Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja
            R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, 
            Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello
            SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn
            WC
  TITLE     Systematic Functional Interrogation of Rare Cancer Variants 
            Identifies Oncogenic Alleles.
  JOURNAL   Cancer Discov. 2016 Jul;6(7):714-26. doi: 
            10.1158/2159-8290.CD-16-0160. Epub 2016 May 4.
  PUBMED    27147599
REFERENCE   2  (bases 1 to 4005)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 13, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4005
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      23..611
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     512..531
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     765..782
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      941..968
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      1060..1146
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     1195..1217
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1209..1226
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1210..1226
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1242..1341
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2557..2656)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2673..2692)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2674..2692)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2697..2713)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2697..2713)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             3144..3935
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
       61 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
      121 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
      181 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
      241 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
      301 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
      361 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
      421 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
      481 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
      541 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
      601 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
      661 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
      721 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
      781 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
      841 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
      901 atacgcgtac cgctagccag gaagagtttg tagaaacgca aaaaggccat ccgtcaggat
      961 ggccttctgc ttagtttgat gcctggcagt ttatggcggg cgtcctgccc gccaccctcc
     1021 gggccgttgc ttcacaacgt tcaaatccgc tcccggcgga tttgtcctac tcaggagagc
     1081 gttcaccgac aaacaacaga taaaacgaaa ggcccagtct tccgactgag cctttcgttt
     1141 tatttgatgc ctggcagttc cctactctcg cgttaacgct agcatggatg ttttcccagt
     1201 cacgacgttg taaaacgacg gccagtctta agctcgggcc ccaaataatg attttatttt
     1261 gactgatagt gacctgttcg ttgcaacaaa ttgatgagca atgctttttt ataatgccaa
     1321 ctttgtacaa aaaagttggc accatgacag ccatcatcaa agagatcgtt agcagaaaca
     1381 aaaggagata tcaagaggat ggattcgact tagacttgac ctatatttat ccaaacatta
     1441 ttgctatggg atttcctgca gaaagacttg aaggcgtata caggaacaat attgatgatg
     1501 tagtaaggtt tttggattca aagcataaaa accattacaa gatatacaat ctttgtgctg
     1561 aaagacatta tgacaccgcc aaatttaatt gcagagttgc acaatatcct tctgaagacc
     1621 ataacccacc acagctagaa cttatcaaac ccttttgtga agatcttgac caatggctaa
     1681 gtgaagatga caatcatgtt gcagcaattc actgtaaagc tggaaaggga cgaactggtg
     1741 taatgatatg tgcatattta ttacatcggg gcaaattttt aaaggcacaa gaggccctag
     1801 atttctatgg ggaagtaagg accagagaca aaaagggagt aactattccc agtcagaggc
     1861 gctatgtgta ttattatagc tacctgttaa agaatcatct ggattataga ccagtggcac
     1921 tgttgtttca caagatgatg tttgaaacta ttccaatgtt cagtggcgga acttgcaatc
     1981 ctcagtttgt ggtctgccag ctaaaggtga agatatattc ctccaattca ggacccacac
     2041 gacgggaaga caagttcatg tactttgagt tccctcagcc gttacctgtg tgtggtgata
     2101 tcaaagtaga gttcttccac aaacagaaca agatgctaaa aaaggacaaa atgtttcact
     2161 tttgggtaaa tacattcttc ataccaggac cagaggaaac ctcagaaaaa gtagaaaatg
     2221 gaagtctatg tgatcaagaa atcgatagca tttgcagtat agagcgtgca gataatgaca
     2281 aggaatatct agtacttact ttaacaaaaa atgatcttga caaagcaaat aaagacaaag
     2341 ccaaccgata cttttctcca aattttaagg tgaagctgta cttcacaaaa acagtagagg
     2401 agccgtcaaa tccagaggct agcagttcaa cttctgtaac accagatgtt agtgacaatg
     2461 aacctgatca ttatagatat tctgacacca ctgactctga tccagagaat gaaccttttg
     2521 atgaagatca gcatacacaa attacaaaag tctgattgcc aactttcttg tacaaagttg
     2581 gcattataag aaagcattgc ttatcaattt gttgcaacga acaggtcact atcagtcaaa
     2641 ataaaatcat tatttgccat ccagctgata tcccctatag tgagtcgtat tacatggtca
     2701 tagctgtttc ctggcagctc tggcccgtgt ctcaaaatct ctgatgttac attgcacaag
     2761 ataaaaatat atcatcatgc ctcctctaga ccagccagga cagaaatgcc tcgacttcgc
     2821 tgctacccaa ggttgccggg tgacgcacac cgtggaaacg gatgaaggca cgaacccagt
     2881 ggacataagc ctgttcggtt cgtaagctgt aatgcaagta gcgtatgcgc tcacgcaact
     2941 ggtccagaac cttgaccgaa cgcagcggtg gtaacggcgc agtggcggtt ttcatggctt
     3001 gttatgactg tttttttggg gtacagtcta tgcctcgggc atccaagcag caagcgcgtt
     3061 acgccgtggg tcgatgtttg atgttatgga gcagcaacga tgttacgcag cagggcagtc
     3121 gccctaaaac aaagttaaac attatgaggg aagcggtgat cgccgaagta tcgactcaac
     3181 tatcagaggt agttggcgtc atcgagcgcc atctcgaacc gacgttgctg gccgtacatt
     3241 tgtacggctc cgcagtggat ggcggcctga agccacacag tgatattgat ttgctggtta
     3301 cggtgaccgt aaggcttgat gaaacaacgc ggcgagcttt gatcaacgac cttttggaaa
     3361 cttcggcttc ccctggagag agcgagattc tccgcgctgt agaagtcacc attgttgtgc
     3421 acgacgacat cattccgtgg cgttatccag ctaagcgcga actgcaattt ggagaatggc
     3481 agcgcaatga cattcttgca ggtatcttcg agccagccac gatcgacatt gatctggcta
     3541 tcttgctgac aaaagcaaga gaacatagcg ttgccttggt aggtccagcg gcggaggaac
     3601 tctttgatcc ggttcctgaa caggatctat ttgaggcgct aaatgaaacc ttaacgctat
     3661 ggaactcgcc gcccgactgg gctggcgatg agcgaaatgt agtgcttacg ttgtcccgca
     3721 tttggtacag cgcagtaacc ggcaaaatcg cgccgaagga tgtcgctgcc gactgggcaa
     3781 tggagcgcct gccggcccag tatcagcccg tcatacttga agctagacag gcttatcttg
     3841 gacaagaaga agatcgcttg gcctcgcgcg cagatcagtt ggaagaattt gtccactacg
     3901 tgaaaggcga gatcaccaag gtagtcggca aataaccctc gagccaccca tgaccaaaat
     3961 cccttaacgt gagttacgcg tcgttccact gagcgtcaga ccccg
//
