LOCUS Exported 3975 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Gateway Donor vector containing MAP2K1, part of the Target Accelerator Plasmid Collection.. ACCESSION . VERSION . KEYWORDS pDONR223_MAP2K1_p.P124S SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3975) AUTHORS Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn WC TITLE Systematic Functional Interrogation of Rare Cancer Variants Identifies Oncogenic Alleles. JOURNAL Cancer Discov. 2016 Jul;6(7):714-26. doi: 10.1158/2159-8290.CD-16-0160. Epub 2016 May 4. PUBMED 27147599 REFERENCE 2 (bases 1 to 3975) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..3975 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 63..80 /label=L4440 /note="L4440 vector, forward primer" terminator 239..266 /label=rrnB T2 terminator /note="transcription terminator T2 from the E. coli rrnB gene" terminator 358..444 /gene="Escherichia coli rrnB" /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" primer_bind 493..515 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 507..524 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 508..524 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" protein_bind 540..639 /gene="mutant version of attL" /label=attL1 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" protein_bind complement(1825..1924) /gene="mutant version of attL" /label=attL2 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" primer_bind complement(1941..1960) /label=T7 /note="T7 promoter, forward primer" promoter complement(1942..1960) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(1965..1981) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(1965..1981) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" CDS 2412..3203 /codon_start=1 /gene="aadA" /product="aminoglycoside adenylyltransferase (Murphy, 1985)" /label=SmR /note="confers resistance to spectinomycin and streptomycin" /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK" rep_origin 3296..3884 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 3785..3804 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc 61 gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg 121 cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca 181 gtgagcgcaa cgcaattaat acgcgtaccg ctagccagga agagtttgta gaaacgcaaa 241 aaggccatcc gtcaggatgg ccttctgctt agtttgatgc ctggcagttt atggcgggcg 301 tcctgcccgc caccctccgg gccgttgctt cacaacgttc aaatccgctc ccggcggatt 361 tgtcctactc aggagagcgt tcaccgacaa acaacagata aaacgaaagg cccagtcttc 421 cgactgagcc tttcgtttta tttgatgcct ggcagttccc tactctcgcg ttaacgctag 481 catggatgtt ttcccagtca cgacgttgta aaacgacggc cagtcttaag ctcgggcccc 541 aaataatgat tttattttga ctgatagtga cctgttcgtt gcaacaaatt gatgagcaat 601 gcttttttat aatgccaact ttgtacaaaa aagttggcac catgcccaag aagaagccga 661 cgcccatcca gctgaacccg gcccccgacg gctctgcagt taacgggacc agctctgcgg 721 agaccaactt ggaggccttg cagaagaagc tggaggagct agagcttgat gagcagcagc 781 gaaagcgcct tgaggccttt cttacccaga agcagaaggt gggagaactg aaggatgacg 841 actttgagaa gatcagtgag ctgggggctg gcaatggcgg tgtggtgttc aaggtctccc 901 acaagccttc tggcctggtc atggccagaa agctaattca tctggagatc aaacccgcaa 961 tccggaacca gatcataagg gagctgcagg ttctgcatga gtgcaactct tcgtacatcg 1021 tgggcttcta tggtgcgttc tacagcgatg gcgagatcag tatctgcatg gagcacatgg 1081 atggaggttc tctggatcaa gtcctgaaga aagctggaag aattcctgaa caaattttag 1141 gaaaagttag cattgctgta ataaaaggcc tgacatatct gagggagaag cacaagatca 1201 tgcacagaga tgtcaagccc tccaacatcc tagtcaactc ccgtggggag atcaagctct 1261 gtgactttgg ggtcagcggg cagctcatcg actccatggc caactccttc gtgggcacaa 1321 ggtcctacat gtcgccagaa agactccagg ggactcatta ctctgtgcag tcagacatct 1381 ggagcatggg actgtctctg gtagagatgg cggttgggag gtatcccatc cctcctccag 1441 atgccaagga gctggagctg atgtttgggt gccaggtgga aggagatgcg gctgagaccc 1501 cacccaggcc aaggaccccc gggaggcccc ttagctcata cggaatggac agccgacctc 1561 ccatggcaaa ttttgagttg ttggattaca tagtcaacga gcctcctcca aaactgccca 1621 gtggagtgtt cagtctggaa tttcaagatt ttgtgaataa atgcttaata aaaaaccccg 1681 cagagagagc agatttgaag caactcatgg ttcatgcttt tatcaagaga tctgatgctg 1741 aggaagtgga ttttgcaggt tggctctgct ccaccatcgg ccttaaccag cccagcacac 1801 caacccatgc tgctggcgtc taattgccaa ctttcttgta caaagttggc attataagaa 1861 agcattgctt atcaatttgt tgcaacgaac aggtcactat cagtcaaaat aaaatcatta 1921 tttgccatcc agctgatatc ccctatagtg agtcgtatta catggtcata gctgtttcct 1981 ggcagctctg gcccgtgtct caaaatctct gatgttacat tgcacaagat aaaaatatat 2041 catcatgcct cctctagacc agccaggaca gaaatgcctc gacttcgctg ctacccaagg 2101 ttgccgggtg acgcacaccg tggaaacgga tgaaggcacg aacccagtgg acataagcct 2161 gttcggttcg taagctgtaa tgcaagtagc gtatgcgctc acgcaactgg tccagaacct 2221 tgaccgaacg cagcggtggt aacggcgcag tggcggtttt catggcttgt tatgactgtt 2281 tttttggggt acagtctatg cctcgggcat ccaagcagca agcgcgttac gccgtgggtc 2341 gatgtttgat gttatggagc agcaacgatg ttacgcagca gggcagtcgc cctaaaacaa 2401 agttaaacat tatgagggaa gcggtgatcg ccgaagtatc gactcaacta tcagaggtag 2461 ttggcgtcat cgagcgccat ctcgaaccga cgttgctggc cgtacatttg tacggctccg 2521 cagtggatgg cggcctgaag ccacacagtg atattgattt gctggttacg gtgaccgtaa 2581 ggcttgatga aacaacgcgg cgagctttga tcaacgacct tttggaaact tcggcttccc 2641 ctggagagag cgagattctc cgcgctgtag aagtcaccat tgttgtgcac gacgacatca 2701 ttccgtggcg ttatccagct aagcgcgaac tgcaatttgg agaatggcag cgcaatgaca 2761 ttcttgcagg tatcttcgag ccagccacga tcgacattga tctggctatc ttgctgacaa 2821 aagcaagaga acatagcgtt gccttggtag gtccagcggc ggaggaactc tttgatccgg 2881 ttcctgaaca ggatctattt gaggcgctaa atgaaacctt aacgctatgg aactcgccgc 2941 ccgactgggc tggcgatgag cgaaatgtag tgcttacgtt gtcccgcatt tggtacagcg 3001 cagtaaccgg caaaatcgcg ccgaaggatg tcgctgccga ctgggcaatg gagcgcctgc 3061 cggcccagta tcagcccgtc atacttgaag ctagacaggc ttatcttgga caagaagaag 3121 atcgcttggc ctcgcgcgca gatcagttgg aagaatttgt ccactacgtg aaaggcgaga 3181 tcaccaaggt agtcggcaaa taaccctcga gccacccatg accaaaatcc cttaacgtga 3241 gttacgcgtc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag 3301 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg 3361 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca 3421 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga 3481 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca 3541 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc 3601 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca 3661 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa 3721 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc 3781 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc 3841 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg 3901 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat 3961 cccctgattc tgtgg //