LOCUS Exported 4611 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Gateway Donor vector containing NFE2L2 , part of the Target Accelerator Plasmid Collection.. ACCESSION . VERSION . KEYWORDS pDONR223_NFE2L2_p.G31V SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4611) AUTHORS Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn WC TITLE Systematic Functional Interrogation of Rare Cancer Variants Identifies Oncogenic Alleles. JOURNAL Cancer Discov. 2016 Jul;6(7):714-26. doi: 10.1158/2159-8290.CD-16-0160. Epub 2016 May 4. PUBMED 27147599 REFERENCE 2 (bases 1 to 4611) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..4611 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 92..109 /label=L4440 /note="L4440 vector, forward primer" terminator 268..295 /label=rrnB T2 terminator /note="transcription terminator T2 from the E. coli rrnB gene" terminator 387..473 /gene="Escherichia coli rrnB" /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" primer_bind 522..544 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 536..553 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 537..553 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" protein_bind 569..668 /gene="mutant version of attL" /label=attL1 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" protein_bind complement(2490..2589) /gene="mutant version of attL" /label=attL2 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" primer_bind complement(2606..2625) /label=T7 /note="T7 promoter, forward primer" promoter complement(2607..2625) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(2630..2646) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(2630..2646) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" CDS 3077..3868 /codon_start=1 /gene="aadA" /product="aminoglycoside adenylyltransferase (Murphy, 1985)" /label=SmR /note="confers resistance to spectinomycin and streptomycin" /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK" rep_origin 3961..4549 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 4450..4469 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga 61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga 121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca 181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc 241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta 301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc 361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa 421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg 481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa 541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac 601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgacaactt tgtacaaaaa 661 agttggcacc atgatggact tggagctgcc gccgccggga ctcccgtccc agcaggacat 721 ggatttgatt gacatacttt ggaggcaaga tatagatctt gtagtaagtc gagaagtatt 781 tgacttcagt cagcgacgga aagagtatga gctggaaaaa cagaaaaaac ttgaaaagga 841 aagacaagaa caactccaaa aggagcaaga gaaagccttt ttcgctcagt tacaactaga 901 tgaagagaca ggtgaatttc tcccaattca gccagcccag cacatccagt cagaaaccag 961 tggatctgcc aactactccc aggttgccca cattcccaaa tcagatgctt tgtactttga 1021 tgactgcatg cagcttttgg cgcagacatt cccgtttgta gatgacaatg aggtttcttc 1081 ggctacgttt cagtcacttg ttcctgatat tcccggtcac atcgagagcc cagtcttcat 1141 tgctactaat caggctcagt cacctgaaac ttctgttgct caggtagccc ctgttgattt 1201 agacggtatg caacaggaca ttgagcaagt ttgggaggag ctattatcca ttcctgagtt 1261 acagtgtctt aatattgaaa atgacaagct ggttgagact accatggttc caagtccaga 1321 agccaaactg acagaagttg acaattatca tttttactca tctataccct caatggaaaa 1381 agaagtaggt aactgtagtc cacattttct taatgctttt gaggattcct tcagcagcat 1441 cctctccaca gaagacccca accagttgac agtgaactca ttaaattcag atgccacagt 1501 caacacagat tttggtgatg aattttattc tgctttcata gctgagccca gtatcagcaa 1561 cagcatgccc tcacctgcta ctttaagcca ttcactctct gaacttctaa atgggcccat 1621 tgatgtttct gatctatcac tttgcaaagc tttcaaccaa aaccaccctg aaagcacagc 1681 agaattcaat gattctgact ccggcatttc actaaacaca agtcccagtg tggcatcacc 1741 agaacactca gtggaatctt ccagctatgg agacacacta cttggcctca gtgattctga 1801 agtggaagag ctagatagtg cccctggaag tgtcaaacag aatggtccta aaacaccagt 1861 acattcttct ggggatatgg tacaaccctt gtcaccatct caggggcaga gcactcacgt 1921 gcatgatgcc caatgtgaga acacaccaga gaaagaattg cctgtaagtc ctggtcatcg 1981 gaaaacccca ttcacaaaag acaaacattc aagccgcttg gaggctcatc tcacaagaga 2041 tgaacttagg gcaaaagctc tccatatccc attccctgta gaaaaaatca ttaacctccc 2101 tgttgttgac ttcaacgaaa tgatgtccaa agagcagttc aatgaagctc aacttgcatt 2161 aattcgggat atacgtagga ggggtaagaa taaagtggct gctcagaatt gcagaaaaag 2221 aaaactggaa aatatagtag aactagagca agatttagat catttgaaag atgaaaaaga 2281 aaaattgctc aaagaaaaag gagaaaatga caaaagcctt cacctactga aaaaacaact 2341 cagcacctta tatctcgaag ttttcagcat gctacgtgat gaagatggaa aaccttattc 2401 tcctagtgaa tactccctgc agcaaacaag agatggcaat gttttccttg ttcccaaaag 2461 taagaagcca gatgttaaga aaaactgatt gccaactttc ttgtacaaag ttggcattat 2521 aagaaagcat tgcttatcaa tttgttgcaa cgaacaggtc actatcagtc aaaataaaat 2581 cattatttgc catccagctg atatccccta tagtgagtcg tattacatgg tcatagctgt 2641 ttcctggcag ctctggcccg tgtctcaaaa tctctgatgt tacattgcac aagataaaaa 2701 tatatcatca tgcctcctct agaccagcca ggacagaaat gcctcgactt cgctgctacc 2761 caaggttgcc gggtgacgca caccgtggaa acggatgaag gcacgaaccc agtggacata 2821 agcctgttcg gttcgtaagc tgtaatgcaa gtagcgtatg cgctcacgca actggtccag 2881 aaccttgacc gaacgcagcg gtggtaacgg cgcagtggcg gttttcatgg cttgttatga 2941 ctgttttttt ggggtacagt ctatgcctcg ggcatccaag cagcaagcgc gttacgccgt 3001 gggtcgatgt ttgatgttat ggagcagcaa cgatgttacg cagcagggca gtcgccctaa 3061 aacaaagtta aacattatga gggaagcggt gatcgccgaa gtatcgactc aactatcaga 3121 ggtagttggc gtcatcgagc gccatctcga accgacgttg ctggccgtac atttgtacgg 3181 ctccgcagtg gatggcggcc tgaagccaca cagtgatatt gatttgctgg ttacggtgac 3241 cgtaaggctt gatgaaacaa cgcggcgagc tttgatcaac gaccttttgg aaacttcggc 3301 ttcccctgga gagagcgaga ttctccgcgc tgtagaagtc accattgttg tgcacgacga 3361 catcattccg tggcgttatc cagctaagcg cgaactgcaa tttggagaat ggcagcgcaa 3421 tgacattctt gcaggtatct tcgagccagc cacgatcgac attgatctgg ctatcttgct 3481 gacaaaagca agagaacata gcgttgcctt ggtaggtcca gcggcggagg aactctttga 3541 tccggttcct gaacaggatc tatttgaggc gctaaatgaa accttaacgc tatggaactc 3601 gccgcccgac tgggctggcg atgagcgaaa tgtagtgctt acgttgtccc gcatttggta 3661 cagcgcagta accggcaaaa tcgcgccgaa ggatgtcgct gccgactggg caatggagcg 3721 cctgccggcc cagtatcagc ccgtcatact tgaagctaga caggcttatc ttggacaaga 3781 agaagatcgc ttggcctcgc gcgcagatca gttggaagaa tttgtccact acgtgaaagg 3841 cgagatcacc aaggtagtcg gcaaataacc ctcgagccac ccatgaccaa aatcccttaa 3901 cgtgagttac gcgtcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc 3961 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc 4021 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt 4081 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt 4141 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc 4201 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa 4261 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac 4321 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg 4381 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga 4441 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact 4501 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa 4561 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt t //