LOCUS       Exported                1033 bp ds-DNA     linear   SYN 19-MAY-2021
DEFINITION  Coexpression plasmid of QuasAr2 and CheRiff for all-optical 
            electrophysiology.
ACCESSION   .
VERSION     .
KEYWORDS    DRH296: FCK-Optopatch2
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1033)
  AUTHORS   Hochbaum DR, Zhao Y, Farhi SL, Klapoetke N, Werley CA, Kapoor V, Zou
            P, Kralj JM, Maclaurin D, Smedemark-Margulies N, Saulnier JL, 
            Boulting GL, Straub C, Cho YK, Melkonian M, Wong GK, Harrison DJ, 
            Murthy VN, Sabatini BL, Boyden ES, Campbell RE, Cohen AE
  TITLE     All-optical electrophysiology in mammalian neurons using engineered 
            microbial rhodopsins.
  JOURNAL   Nat Methods. 2014 Aug;11(8):825-33. doi: 10.1038/nmeth.3000. Epub 
            2014 Jun 22.
  PUBMED    24952910
REFERENCE   2  (bases 1 to 1033)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 19, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..1033
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             347..403
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
ORIGIN
        1 cgagcggatg taccccgagg acggtgccct gaagggcaag atcaagatga ggctgaagct
       61 gaaggacggc ggccactaca cctccgaggt caagaccacc tacaaggcca agaagcccgt
      121 gcagctgccc ggcgcctaca tcgtcgacat caagttggac atcacctccc acaacgagga
      181 ctacaccatc gtggaacagt acgaacgcgc cgagggccgc cactccaccg gcggcatgga
      241 cgagctgtac aagagtagaa tcacaagcga aggcgagtac atccccctgg atcaaataga
      301 cataaatgta ggtggatttt gttatgagaa tgaagtgggc tccggagcca cgaacttctc
      361 tctgttaaag caagcaggag acgtggaaga aaaccccggt cccggcggag ctcctgctcc
      421 agacgctcac agcgccccac ctggaaacga ttctgccgga ggcagtgagt accatgcccc
      481 agctggatat caagtgaatc caccctacca ccccgtgcat gggtatgagg aacagtgcag
      541 ctccatctac atctactatg gggccctgtg ggagcaggaa acagctaggg gcttccagtg
      601 gtttgccgtg ttcctgtctg ccctgtttct ggctttctac ggctggcacg cctataaggc
      661 cagcgtggga tgggaggaag tgtacgtgtg ctccgtggag ctgatcaaag tgattctgga
      721 gatctatttc gagttcacca gtcctgctat gctgttcctg tacggaggga acattacccc
      781 atggctgaga tatgccgaat ggctgctgac atgtcccgtg atcctgattc atctgtctaa
      841 catcaccggc ctgagtgagg catacaataa gcggacaatg gctctgctgg tgtccgacct
      901 gggaactatt tgcatgggag tgacagccgc tctggccact gggtgggtga agtggctgtt
      961 ttactgtatc ggcctggtgt atggaaccca gacattctac aacgctggaa tcatctacgt
     1021 ggagtcttac tat
//
