LOCUS Exported 3435 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Gateway Donor vector containing VHL , part of the Target Accelerator Plasmid Collection.. ACCESSION . VERSION . KEYWORDS pDONR223_VHL_WT SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3435) AUTHORS Kim E, Ilic N, Shrestha Y, Zou L, Kamburov A, Zhu C, Yang X, Lubonja R, Tran N, Nguyen C, Lawrence MS, Piccioni F, Bagul M, Doench JG, Chouinard CR, Wu X, Hogstrom L, Natoli T, Tamayo P, Horn H, Corsello SM, Lage K, Root DE, Subramanian A, Golub TR, Getz G, Boehm JS, Hahn WC TITLE Systematic Functional Interrogation of Rare Cancer Variants Identifies Oncogenic Alleles. JOURNAL Cancer Discov. 2016 Jul;6(7):714-26. doi: 10.1158/2159-8290.CD-16-0160. Epub 2016 May 4. PUBMED 27147599 REFERENCE 2 (bases 1 to 3435) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..3435 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 43..65 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 57..74 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 58..74 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" protein_bind 90..189 /gene="mutant version of attL" /label=attL1 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" protein_bind complement(835..934) /gene="mutant version of attL" /label=attL2 /bound_moiety="LR Clonase(TM)" /note="recombination site for the Gateway(R) LR reaction" primer_bind complement(951..970) /label=T7 /note="T7 promoter, forward primer" promoter complement(952..970) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(975..991) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(975..991) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" CDS 1422..2213 /codon_start=1 /gene="aadA" /product="aminoglycoside adenylyltransferase (Murphy, 1985)" /label=SmR /note="confers resistance to spectinomycin and streptomycin" /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK" rep_origin 2306..2894 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 2795..2814 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 3048..3065 /label=L4440 /note="L4440 vector, forward primer" terminator 3224..3251 /label=rrnB T2 terminator /note="transcription terminator T2 from the E. coli rrnB gene" terminator 3343..3429 /gene="Escherichia coli rrnB" /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" ORIGIN 1 ggcagttccc tactctcgcg ttaacgctag catggatgtt ttcccagtca cgacgttgta 61 aaacgacggc cagtcttaag ctcgggcccc aaataatgat tttattttga ctgatagtga 121 cctgttcgtt gcaacaaatt gatgagcaat gcttttttat aatgccaact ttgtacaaaa 181 aagttggcac catgccccgg agggcggaga actgggacga ggccgaggta ggcgcggagg 241 aggcaggcgt cgaagagtac ggccctgaag aagacggcgg ggaggagtcg ggcgccgagg 301 agtccggccc ggaagagtcc ggcccggagg aactgggcgc cgaggaggag atggaggccg 361 ggcggccgcg gcccgtgctg cgctcggtga actcgcgcga gccctcccag gtcatcttct 421 gcaatcgcag tccgcgcgtc gtgctgcccg tatggctcaa cttcgacggc gagccgcagc 481 cctacccaac gctgccgcct ggcacgggcc gccgcatcca cagctaccga ggtcaccttt 541 ggctcttcag agatgcaggg acacacgatg ggcttctggt taaccaaact gaattatttg 601 tgccatctct caatgttgac ggacagccta tttttgccaa tatcacactg ccagtgtata 661 ctctgaaaga gcgatgcctc caggttgtcc ggagcctagt caagcctgag aattacagga 721 gactggacat cgtcaggtcg ctctacgaag atctggaaga ccacccaaat gtgcagaaag 781 acctggagcg gctgacacag gagcgcattg cacatcaacg gatgggagat tgattgccaa 841 ctttcttgta caaagttggc attataagaa agcattgctt atcaatttgt tgcaacgaac 901 aggtcactat cagtcaaaat aaaatcatta tttgccatcc agctgatatc ccctatagtg 961 agtcgtatta catggtcata gctgtttcct ggcagctctg gcccgtgtct caaaatctct 1021 gatgttacat tgcacaagat aaaaatatat catcatgcct cctctagacc agccaggaca 1081 gaaatgcctc gacttcgctg ctacccaagg ttgccgggtg acgcacaccg tggaaacgga 1141 tgaaggcacg aacccagtgg acataagcct gttcggttcg taagctgtaa tgcaagtagc 1201 gtatgcgctc acgcaactgg tccagaacct tgaccgaacg cagcggtggt aacggcgcag 1261 tggcggtttt catggcttgt tatgactgtt tttttggggt acagtctatg cctcgggcat 1321 ccaagcagca agcgcgttac gccgtgggtc gatgtttgat gttatggagc agcaacgatg 1381 ttacgcagca gggcagtcgc cctaaaacaa agttaaacat tatgagggaa gcggtgatcg 1441 ccgaagtatc gactcaacta tcagaggtag ttggcgtcat cgagcgccat ctcgaaccga 1501 cgttgctggc cgtacatttg tacggctccg cagtggatgg cggcctgaag ccacacagtg 1561 atattgattt gctggttacg gtgaccgtaa ggcttgatga aacaacgcgg cgagctttga 1621 tcaacgacct tttggaaact tcggcttccc ctggagagag cgagattctc cgcgctgtag 1681 aagtcaccat tgttgtgcac gacgacatca ttccgtggcg ttatccagct aagcgcgaac 1741 tgcaatttgg agaatggcag cgcaatgaca ttcttgcagg tatcttcgag ccagccacga 1801 tcgacattga tctggctatc ttgctgacaa aagcaagaga acatagcgtt gccttggtag 1861 gtccagcggc ggaggaactc tttgatccgg ttcctgaaca ggatctattt gaggcgctaa 1921 atgaaacctt aacgctatgg aactcgccgc ccgactgggc tggcgatgag cgaaatgtag 1981 tgcttacgtt gtcccgcatt tggtacagcg cagtaaccgg caaaatcgcg ccgaaggatg 2041 tcgctgccga ctgggcaatg gagcgcctgc cggcccagta tcagcccgtc atacttgaag 2101 ctagacaggc ttatcttgga caagaagaag atcgcttggc ctcgcgcgca gatcagttgg 2161 aagaatttgt ccactacgtg aaaggcgaga tcaccaaggt agtcggcaaa taaccctcga 2221 gccacccatg accaaaatcc cttaacgtga gttacgcgtc gttccactga gcgtcagacc 2281 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct 2341 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa 2401 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag 2461 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc 2521 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg 2581 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca 2641 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat 2701 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg 2761 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc 2821 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc 2881 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc 2941 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg 3001 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga 3061 gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc 3121 attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa 3181 ttaatacgcg taccgctagc caggaagagt ttgtagaaac gcaaaaaggc catccgtcag 3241 gatggccttc tgcttagttt gatgcctggc agtttatggc gggcgtcctg cccgccaccc 3301 tccgggccgt tgcttcacaa cgttcaaatc cgctcccggc ggatttgtcc tactcaggag 3361 agcgttcacc gacaaacaac agataaaacg aaaggcccag tcttccgact gagcctttcg 3421 ttttatttga tgcct //