LOCUS       Exported                7286 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pMXs-c-Myc-IP
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7286)
  AUTHORS   Takahashi K, Yamanaka S
  TITLE     Induction of pluripotent stem cells from mouse embryonic and adult 
            fibroblast cultures by defined factors.
  JOURNAL   Cell. 2006 Aug 25. 126(4):663-76.
  PUBMED    16904174
REFERENCE   2  (bases 1 to 7286)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..7286
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     LTR             13..606
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    669..1026
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1085..1501
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1431..1453
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1469..1485
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     misc_feature    1511..1885
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     1823..1839
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     protein_bind    1916..1940
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3297..3321)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    3369..3955
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3536..3553)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     3763..3782
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             3956..4555
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3956..3975)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4452..4472
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             4782..5251
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(5381..5398)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5552..6140)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5632..5651)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(6304..7164)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6927..6946
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7165..7269)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tgccacctgc ataatgaaag accccacctg taggtttggc aagctagctt aagtaacgcc
       61 attttgcaag gcatggaaaa atacataact gagaatagaa aagttcagat caaggtcagg
      121 aacagatgga acagctgaat atgggccaaa caggatatct gtggtaagca gttcctgccc
      181 cggctcaggg ccaagaacag atggaacagc tgaatatggg ccaaacagga tatctgtggt
      241 aagcagttcc tgccccggct cagggccaag aacagatggt ccccagatgc ggtccagccc
      301 tcagcagttt ctagagaacc atcagatgtt tccagggtgc cccaaggacc tgaaatgacc
      361 ctgtgcctta tttgaactaa ccaatcagtt cgcttctcgc ttctgttcgc gcgcttctgc
      421 tccccgagct caataaaaga gcccacaacc cctcactcgg cgcgccagtc ctccgattga
      481 ctgagtcgcc cgggtacccg tgtatccaat aaaccctctt gcagttgcat ccgacttgtg
      541 gtctcgctgt tccttgggag ggtctcctct gagtgattga ctacccgtca gcgggggtct
      601 ttcatttggg ggctcgtccg ggatcgggag acccctgccc agggaccacc gacccaccac
      661 cgggaggtaa gctggccagc aacttatctg tgtctgtccg attgtctagt gtctatgact
      721 gattttatgc gcctgcgtcg gtactagtta gctaactagc tctgtatctg gcggacccgt
      781 ggtggaactg acgagttcgg aacacccggc cgcaaccctg ggagacgtcc cagggacttc
      841 gggggccgtt tttgtggccc gacctgagtc caaaaatccc gatcgttttg gactctttgg
      901 tgcacccccc taataggagg gatatgtggt tctggtagga gacgagaacc taaaacagtt
      961 cccgcctccg tctgaatttt tgctttcggt ttgggaccga agccgcgccg cgcgtcttgt
     1021 ctgctgcagc atcgttctgt gttgtctctg tctgactgtg tttctgtatt tgtctgaaaa
     1081 ttagggccag actgttacca ctcccttaag tttgacctta ggtcactgga aagatgtcga
     1141 gcggatcgct cacaaccagt cggtagatgt caagaagaga cgttgggtta ccttctgctc
     1201 tgcagaatgg ccaaccttta acgtcggatg gccgcgagac ggcaccttta accgagacct
     1261 catcacccag gttaagatca aggtcttttc acctggcccg catggacacc cagaccaggt
     1321 cccctacatc gtgacctggg aagccttggc ttttgacccc cctccctggg tcaagccctt
     1381 tgtacaccct aagcctccgc ctcctcttcc tccatccgcc ccgtctctcc cccttgaacc
     1441 tcctcgttcg accccgcctc gatcctccct ttatccagcc ctcactcctt ctctaggcgc
     1501 ccccatatgg ccatatgaga tcttatatgg ggcacccccg ccccttgtaa acttccctga
     1561 ccctgacatg acaagagtta ctaacagccc ctctctccaa gctcacttac aggctctcta
     1621 cttagtccag cacgaagtct ggagacctct ggcggcagcc taccaagaac aactggaccg
     1681 accggtggta cctcaccctt accgagtcgg cgacacagtg tgggtccgcc gacaccagac
     1741 taagaaccta gaacctcgct ggaaaggacc ttacacagtc ctgctgacca cccccaccgc
     1801 cctcaaagta gacggcatcg cagcttggat acacgccgcc cacgtgaagg ctgccgaccc
     1861 cgggggtgga ccatcctcta gactgccgga tctagctagt taattaagga tcatcacaag
     1921 tttgtacaaa aaagcaggct ccgcggccgc ccccttcacc atgcccctca acgtgaactt
     1981 caccaacagg aactatgacc tcgactacga ctccgtacag ccctatttca tctgcgacga
     2041 ggaagagaat ttctatcacc agcaacagca gagcgagctg cagccgcccg cgcccagtga
     2101 ggatatctgg aagaaattcg agctgcttcc caccccgccc ctgtccccga gccgccgctc
     2161 cgggctctgc tctccatcct atgttgcggt cgctacgtcc ttctccccaa gggaagacga
     2221 tgacggcggc ggtggcaact tctccaccgc cgatcagctg gagatgatga ccgagttact
     2281 tggaggagac atggtgaacc agagcttcat ctgcgatcct gacgacgaga ccttcatcaa
     2341 gaacatcatc atccaggact gtatgtggag cggtttctca gccgctgcca agctggtctc
     2401 ggagaagctg gcctcctacc aggctgcgcg caaagacagc accagcctga gccccgcccg
     2461 cgggcacagc gtctgctcca cctccagcct gtacctgcag gacctcaccg ccgccgcgtc
     2521 cgagtgcatt gacccctcag tggtctttcc ctacccgctc aacgacagca gctcgcccaa
     2581 atcctgtacc tcgtccgatt ccacggcctt ctctccttcc tcggactcgc tgctgtcctc
     2641 cgagtcctcc ccacgggcca gccctgagcc cctagtgctg catgaggaga caccgcccac
     2701 caccagcagc gactctgaag aagagcaaga agatgaggaa gaaattgatg tggtgtctgt
     2761 ggagaagagg caaacccctg ccaagaggtc ggagtcgggc tcatctccat cccgaggcca
     2821 cagcaaacct ccgcacagcc cactggtcct caagaggtgc cacgtctcca ctcaccagca
     2881 caactacgcc gcacccccct ccacaaggaa ggactatcca gctgccaaga gggccaagtt
     2941 ggacagtggc agggtcctga agcagatcag caacaaccgc aagtgctcca gccccaggtc
     3001 ctcagacacg gaggaaaacg acaagaggcg gacacacaac gtcttggaac gtcagaggag
     3061 gaacgagctg aagcgcagct tttttgccct gcgtgaccag atccctgaat tggaaaacaa
     3121 cgaaaaggcc cccaaggtag tgatcctcaa aaaagccacc gcctacatcc tgtccattca
     3181 agcagacgag cacaagctca cctctgaaaa ggacttattg aggaaacgac gagaacagtt
     3241 gaaacacaaa ctcgaacagc ttcgaaactc tggtgcataa aagggtgggc gcgccgaccc
     3301 agctttcttg tacaaagtgg tgattcgagc ggccgctcga taagcttgat atcgaattcc
     3361 gccccccccc cctctccctc ccccccccct aacgttactg gccgaagccg cttggaataa
     3421 ggccggtgtg cgtttgtcta tatgttattt tccaccatat tgccgtcttt tggcaatgtg
     3481 agggcccgga aacctggccc tgtcttcttg acgagcattc ctaggggtct ttcccctctc
     3541 gccaaaggaa tgcaaggtct gttgaatgtc gtgaaggaag cagttcctct ggaagcttct
     3601 tgaagacaaa caacgtctgt agcgaccctt tgcaggcagc ggaacccccc acctggcgac
     3661 aggtgcctct gcggccaaaa gccacgtgta taagatacac ctgcaaaggc ggcacaaccc
     3721 cagtgccacg ttgtgagttg gatagttgtg gaaagagtca aatggctctc ctcaagcgta
     3781 ttcaacaagg ggctgaagga tgcccagaag gtaccccatt gtatgggatc tgatctgggg
     3841 cctcggtgca catgctttac atgtgtttag tcgaggttaa aaaaacgtct aggccccccg
     3901 aaccacgggg acgtggtttt cctttgaaaa acacgatgat aatatggcca caaccatgac
     3961 cgagtacaag cccacggtgc gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac
     4021 cctcgccgcc gcgttcgccg actaccccgc cacgcgccac accgtcgatc cggaccgcca
     4081 catcgagcgg gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg
     4141 caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc cggagagcgt
     4201 cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga gcggttcccg
     4261 gctggccgcg cagcaacaga tggaaggcct cctggcgccg caccggccca aggagcccgc
     4321 gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc
     4381 cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg gtgcccgcct tcctggagac
     4441 ctccgcgccc cgcaacctcc ccttctacga gcggctcggc ttcaccgtca ccgccgacgt
     4501 cgaggtgccc gaaggaccgc gcacctggtg catgacccgc aagcccggtg cctgacgccc
     4561 gccccacgac ccgcagcgcc cgaccgaaag gagcgcacga ccccatgcat cgtcgacgat
     4621 aaaataaaag attttattta gtctccagaa aaagggggga atgaaagacc ccacctgtag
     4681 gtttggcaag ctagcttaag taacgccatt ttgcaaggca tggaaaaata cataactgag
     4741 aatagagaag ttcagatcaa ggtcaggaac agatggaaca gctgaatatg ggccaaacag
     4801 gatatctgtg gtaagcagtt cctgccccgg ctcagggcca agaacagatg gaacagctga
     4861 atatgggcca aacaggatat ctgtggtaag cagttcctgc cccggctcag ggccaagaac
     4921 agatggtccc cagatgcggt ccagccctca gcagtttcta gagaaccatc agatgtttcc
     4981 agggtgcccc aaggacctga aatgaccctg tgccttattt gaactaacca atcagttcgc
     5041 ttctcgcttc tgttcgcgcg cttctgctcc ccgagctcaa taaaagagcc cacaacccct
     5101 cactcggggc gccagtcctc cgattgactg agtcgcccgg gtacccgtgt atccaataaa
     5161 ccctcttgca gttgcatccg acttgtggtc tcgctgttcc ttgggagggt ctcctctgag
     5221 tgattgacta cccgtcagcg ggggtctttc acatgcagca tgtatcaaaa ttaatttggt
     5281 tttttttctt aagtatttac attaaatggc catagttgca ttaatgaatc ggccaacgcg
     5341 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc
     5401 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
     5461 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     5521 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     5581 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     5641 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     5701 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     5761 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     5821 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     5881 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     5941 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat
     6001 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     6061 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     6121 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     6181 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
     6241 agatcctttt gcggccggcc gcaaatcaat ctaaagtata tatgagtaaa cttggtctga
     6301 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
     6361 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
     6421 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
     6481 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
     6541 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
     6601 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
     6661 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
     6721 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
     6781 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
     6841 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
     6901 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
     6961 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
     7021 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
     7081 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
     7141 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
     7201 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
     7261 ggttccgcgc acatttcccc gaaaag
//
