LOCUS       Exported                8177 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pbabe-hTERT+p53DD
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8177)
  AUTHORS   Kendall SD, Linardic CM, Adam SJ, Counter CM
  TITLE     A network of genetic events sufficient to convert normal human cells
            to a tumorigenic state.
  JOURNAL   Cancer Res. 2005 Nov 1. 65(21):9824-8.
  PUBMED    16267004
REFERENCE   2  (bases 1 to 8177)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8177
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     LTR             18..487
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    552..751
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             952..1368
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1298..1320
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1336..1352
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     CDS             1448..4846
                     /codon_start=1
                     /gene="TERT"
                     /product="human telomerase reverse transcriptase, the 
                     catalytic subunit of telomerase"
                     /label=hTERT
                     /note="prevents telomere shortening and allows 
                     postsenescent cells to proliferate beyond crisis (Counter 
                     et al., 1998); also known as EST2"
                     /translation="MPRAPRCRAVRSLLRSHYREVLPLATFVRRLGPQGWRLVQRGDPA
                     AFRALVAQCLVCVPWDARPPPAAPSFRQVSCLKELVARVLQRLCERGAKNVLAFGFALL
                     DGARGGPPEAFTTSVRSYLPNTVTDALRGSGAWGLLLRRVGDDVLVHLLARCALFVLVA
                     PSCAYQVCGPPLYQLGAATQARPPPHASGPRRRLGCERAWNHSVREAGVPLGLPAPGAR
                     RRGGSASRSLPLPKRPRRGAAPEPERTPVGQGSWAHPGRTRGPSDRGFCVVSPARPAEE
                     ATSLEGALSGTRHSHPSVGRQHHAGPPSTSRPPRPWDTPCPPVYAETKHFLYSSGDKEQ
                     LRPSFLLSSLRPSLTGARRLVETIFLGSRPWMPGTPRRLPRLPQRYWQMRPLFLELLGN
                     HAQCPYGVLLKTHCPLRAAVTPAAGVCAREKPQGSVAAPEEEDTDPRRLVQLLRQHSSP
                     WQVYGFVRACLRRLVPPGLWGSRHNERRFLRNTKKFISLGKHAKLSLQELTWKMSVRGC
                     AWLRRSPGVGCVPAAEHRLREEILAKFLHWLMSVYVVELLRSFFYVTETTFQKNRLFFY
                     RKSVWSKLQSIGIRQHLKRVQLRELSEAEVRQHREARPALLTSRLRFIPKPDGLRPIVN
                     MDYVVGARTFRREKRAERLTSRVKALFSVLNYERARRPGLLGASVLGLDDIHRAWRTFV
                     LRVRAQDPPPELYFVKVDVTGAYDTIPQDRLTEVIASIIKPQNTYCVRRYAVVQKAAHG
                     HVRKAFKSHVSTLTDLQPYMRQFVAHLQETSPLRDAVVIEQSSSLNEASSGLFDVFLRF
                     MCHHAVRIRGKSYVQCQGIPQGSILSTLLCSLCYGDMENKLFAGIRRDGLLLRLVDDFL
                     LVTPHLTHAKTFLRTLVRGVPEYGCVVNLRKTVVNFPVEDEALGGTAFVQMPAHGLFPW
                     CGLLLDTRTLEVQSDYSSYARTSIRASLTFNRGFKAGRNMRRKLFGVLRLKCHSLFLDL
                     QVNSLQTVCTNIYKILLLQAYRFHACVLQLPFHQQVWKNPTFFLRVISDTASLCYSILK
                     AKNAGMSLGAKGAAGPLPSEAVQWLCHQAFLLKLTRHRVTYVPLLGSLRTAQTQLSRKL
                     PGTTLTALEAAANPALPSDFKTILD"
     primer_bind     complement(4858..4878)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4863..5192
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5043..5178
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5105..5124
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     LTR             5681..6150
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(6280..6297)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6451..7039)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6531..6550)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(7199..8059)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7822..7841
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8060..8164)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 aaagtgccac ctgcagcctg aatatgggcc aaacaggata tctgtggtaa gcagttcctg
       61 ccccggctca gggccaagaa cagatggaac agctgaatat gggccaaaca ggatatctgt
      121 ggtaagcagt tcctgccccg gctcagggcc aagaacagat ggtccccaga tgcggtccag
      181 ccctcagcag tttctagaga accatcagat gtttccaggg tgccccaagg acctgaaatg
      241 accctgtgcc ttatttgaac taaccaatca gttcgcttct cgcttctgtt cgcgcgcttc
      301 tgctccccga gctcaataaa agagcccaca acccctcact cggggcgcca gtcctccgat
      361 tgactgagtc gcccgggtac ccgtgtatcc aataaaccct cttgcagttg catccgactt
      421 gtggtctcgc tgttccttgg gagggtctcc tctgagtgat tgactacccg tcagcggggg
      481 tctttcattt gggggctcgt ccgggatcgg gagacccctg cccagggacc accgacccac
      541 caccgggagg caagctggcc agcaacttat ctgtgtctgt ccgattgtct agtgtctatg
      601 actgatttta tgcgcctgcg tcggtactag ttagctaact agctctgtat ctggcggacc
      661 cgtggtggaa ctgacgagtt ctgaacaccc ggccgcaacc ctgggagacg tcccagggac
      721 tttgggggcc gtttttgtgg cccgacctga ggaagggagt cgatgtggaa tccgaccccg
      781 tcaggatatg tggttctggt aggagacgag aacctaaaac agttcccgcc tccgtctgaa
      841 tttttgcttt cggtttggaa ccgaagccgc gcgtcttgtc tgctgcagcg ctgcagcatc
      901 gttctgtgtt gtctctgtct gactgtgttt ctgtatttgt ctgaaaatta gggccagact
      961 gttaccactc ccttaagttt gaccttaggt cactggaaag atgtcgagcg gatcgctcac
     1021 aaccagtcgg tagatgtcaa gaagagacgt tgggttacct tctgctctgc agaatggcca
     1081 acctttaacg tcggatggcc gcgagacggc acctttaacc gagacctcat cacccaggtt
     1141 aagatcaagg tcttttcacc tggcccgcat ggacacccag accaggtccc ctacatcgtg
     1201 acctgggaag ccttggcttt tgacccccct ccctgggtca agccctttgt acaccctaag
     1261 cctccgcctc ctcttcctcc atccgccccg tctctccccc ttgaacctcc tcgttcgacc
     1321 ccgcctcgat cctcccttta tccagccctc actccttctc taggcgccgg ccggatccca
     1381 gtgtggtggt acgtaggaat tcgtcctgct gcgcacgtgg gaagccctgg ccccggccac
     1441 ccccgcgatg ccgcgcgctc cccgctgccg agccgtgcgc tccctgctgc gcagccacta
     1501 ccgcgaggtg ctgccgctgg ccacgttcgt gcggcgcctg gggccccagg gctggcggct
     1561 ggtgcagcgc ggggacccgg cggctttccg cgcgctggtg gcccagtgcc tggtgtgcgt
     1621 gccctgggac gcacggccgc cccccgccgc cccctccttc cgccaggtgt cctgcctgaa
     1681 ggagctggtg gcccgagtgc tgcagaggct gtgcgagcgc ggcgcgaaga acgtgctggc
     1741 cttcggcttc gcgctgctgg acggggcccg cgggggcccc cccgaggcct tcaccaccag
     1801 cgtgcgcagc tacctgccca acacggtgac cgacgcactg cgggggagcg gggcgtgggg
     1861 gctgctgctg cgccgcgtgg gcgacgacgt gctggttcac ctgctggcac gctgcgcgct
     1921 ctttgtgctg gtggctccca gctgcgccta ccaggtgtgc gggccgccgc tgtaccagct
     1981 cggcgctgcc actcaggccc ggcccccgcc acacgctagt ggaccccgaa ggcgtctggg
     2041 atgcgaacgg gcctggaacc atagcgtcag ggaggccggg gtccccctgg gcctgccagc
     2101 cccgggtgcg aggaggcgcg ggggcagtgc cagccgaagt ctgccgttgc ccaagaggcc
     2161 caggcgtggc gctgcccctg agccggagcg gacgcccgtt gggcaggggt cctgggccca
     2221 cccgggcagg acgcgtggac cgagtgaccg tggtttctgt gtggtgtcac ctgccagacc
     2281 cgccgaagaa gccacctctt tggagggtgc gctctctggc acgcgccact cccacccatc
     2341 cgtgggccgc cagcaccacg cgggcccccc atccacatcg cggccaccac gtccctggga
     2401 cacgccttgt cccccggtgt acgccgagac caagcacttc ctctactcct caggcgacaa
     2461 ggagcagctg cggccctcct tcctactcag ctctctgagg cccagcctga ctggcgctcg
     2521 gaggctcgtg gagaccatct ttctgggttc caggccctgg atgccaggga ctccccgcag
     2581 gttgccccgc ctgccccagc gctactggca aatgcggccc ctgtttctgg agctgcttgg
     2641 gaaccacgcg cagtgcccct acggggtgct cctcaagacg cactgcccgc tgcgagctgc
     2701 ggtcacccca gcagccggtg tctgtgcccg ggagaagccc cagggctctg tggcggcccc
     2761 cgaggaggag gacacagacc cccgtcgcct ggtgcagctg ctccgccagc acagcagccc
     2821 ctggcaggtg tacggcttcg tgcgggcctg cctgcgccgg ctggtgcccc caggcctctg
     2881 gggctccagg cacaacgaac gccgcttcct caggaacacc aagaagttca tctccctggg
     2941 gaagcatgcc aagctctcgc tgcaggagct gacgtggaag atgagcgtgc ggggctgcgc
     3001 ttggctgcgc aggagcccag gggttggctg tgttccggcc gcagagcacc gtctgcgtga
     3061 ggagatcctg gccaagttcc tgcactggct gatgagtgtg tacgtcgtcg agctgctcag
     3121 gtctttcttt tatgtcacgg agaccacgtt tcaaaagaac aggctctttt tctaccggaa
     3181 gagtgtctgg agcaagttgc aaagcattgg aatcagacag cacttgaaga gggtgcagct
     3241 gcgggagctg tcggaagcag aggtcaggca gcatcgggaa gccaggcccg ccctgctgac
     3301 gtccagactc cgcttcatcc ccaagcctga cgggctgcgg ccgattgtga acatggacta
     3361 cgtcgtggga gccagaacgt tccgcagaga aaagagggcc gagcgtctca cctcgagggt
     3421 gaaggcactg ttcagcgtgc tcaactacga gcgggcgcgg cgccccggcc tcctgggcgc
     3481 ctctgtgctg ggcctggacg atatccacag ggcctggcgc accttcgtgc tgcgtgtgcg
     3541 ggcccaggac ccgccgcctg agctgtactt tgtcaaggtg gatgtgacgg gcgcgtacga
     3601 caccatcccc caggacaggc tcacggaggt catcgccagc atcatcaaac cccagaacac
     3661 gtactgcgtg cgtcggtatg ccgtggtcca gaaggccgcc catgggcacg tccgcaaggc
     3721 cttcaagagc cacgtctcta ccttgacaga cctccagccg tacatgcgac agttcgtggc
     3781 tcacctgcag gagaccagcc cgctgaggga tgccgtcgtc atcgagcaga gctcctccct
     3841 gaatgaggcc agcagtggcc tcttcgacgt cttcctacgc ttcatgtgcc accacgccgt
     3901 gcgcatcagg ggcaagtcct acgtccagtg ccaggggatc ccgcagggct ccatcctctc
     3961 cacgctgctc tgcagcctgt gctacggcga catggagaac aagctgtttg cggggattcg
     4021 gcgggacggg ctgctcctgc gtttggtgga tgatttcttg ttggtgacac ctcacctcac
     4081 ccacgcgaaa accttcctca ggaccctggt ccgaggtgtc cctgagtatg gctgcgtggt
     4141 gaacttgcgg aagacagtgg tgaacttccc tgtagaagac gaggccctgg gtggcacggc
     4201 ttttgttcag atgccggccc acggcctatt cccctggtgc ggcctgctgc tggatacccg
     4261 gaccctggag gtgcagagcg actactccag ctatgcccgg acctccatca gagccagtct
     4321 caccttcaac cgcggcttca aggctgggag gaacatgcgt cgcaaactct ttggggtctt
     4381 gcggctgaag tgtcacagcc tgtttctgga tttgcaggtg aacagcctcc agacggtgtg
     4441 caccaacatc tacaagatcc tcctgctgca ggcgtacagg tttcacgcat gtgtgctgca
     4501 gctcccattt catcagcaag tttggaagaa ccccacattt ttcctgcgcg tcatctctga
     4561 cacggcctcc ctctgctact ccatcctgaa agccaagaac gcagggatgt cgctgggggc
     4621 caagggcgcc gccggccctc tgccctccga ggccgtgcag tggctgtgcc accaagcatt
     4681 cctgctcaag ctgactcgac accgtgtcac ctacgtgcca ctcctggggt cactcaggac
     4741 agcccagacg cagctgagtc ggaagctccc ggggacgacg ctgactgccc tggaggccgc
     4801 agccaacccg gcactgccct cagacttcaa gaccatcctg gactgagtcg accctgtgga
     4861 atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
     4921 gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca
     4981 gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc
     5041 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt
     5101 tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag
     5161 gaggcttttt tggaggccta ggcttttgca aaaagcttga ctggatgact gccatggagg
     5221 agtcacagtc ggatatcagc ctcgagagag cgctgcccac ctgcacaagc gcctctcccc
     5281 cgcaaaagaa aaaaccactt gatggagagt atttcaccct caagatccgc gggcgtaaac
     5341 gcttcgagat gttccgggag ctgaatgagg ccttagagtt aaaggatgcc catgctacag
     5401 aggagtctgg agacagcagg gctcactcca gctacctgaa gaccaagaag ggccagtcta
     5461 cttcccgcca taaaaaaaca atggtcaaga aagtggggcc tgactcagac tgaatcgata
     5521 aaataaaaga ttttatttag tctccagaaa aaggggggaa tgaaagaccc cacctgtagg
     5581 tttggcaagc tagcttaagt aacgccattt tgcaaggcat ggaaaaatac ataactgaga
     5641 atagagaagt tcagatcaag gtcaggaaca gatggaacag ctgaatatgg gccaaacagg
     5701 atatctgtgg taagcagttc ctgccccggc tcagggccaa gaacagatgg aacagctgaa
     5761 tatgggccaa acaggatatc tgtggtaagc agttcctgcc ccggctcagg gccaagaaca
     5821 gatggtcccc agatgcggtc cagccctcag cagtttctag agaaccatca gatgtttcca
     5881 gggtgcccca aggacctgaa atgaccctgt gccttatttg aactaaccaa tcagttcgct
     5941 tctcgcttct gttcgcgcgc ttctgctccc cgagctcaat aaaagagccc acaacccctc
     6001 actcggggcg ccagtcctcc gattgactga gtcgcccggg tacccgtgta tccaataaac
     6061 cctcttgcag ttgcatccga cttgtggtct cgctgttcct tgggagggtc tcctctgagt
     6121 gattgactac ccgtcagcgg gggtctttca catgcagcat gtatcaaaat taatttggtt
     6181 ttttttctta agtatttaca ttaaatggcc atagttgcat taatgaatcg gccaacgcgc
     6241 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
     6301 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     6361 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     6421 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     6481 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     6541 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     6601 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     6661 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     6721 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     6781 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     6841 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
     6901 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     6961 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     7021 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     7081 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     7141 gatccttttg cggccgcaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
     7201 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     7261 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     7321 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
     7381 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     7441 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
     7501 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
     7561 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
     7621 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
     7681 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
     7741 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
     7801 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
     7861 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
     7921 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
     7981 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
     8041 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
     8101 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
     8161 cgcgcacatt tccccga
//
