LOCUS Exported 5666 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pCMV-HA-MyD88 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5666) AUTHORS Jiang Z, Georgel P, Li C, Choe J, Crozat K, Rutschmann S, Du X, Bigby T, Mudd S, Sovath S, Wilson IA, Olson A, Beutler B TITLE Details of Toll-like receptor:adapter interaction revealed by germ-line mutagenesis. JOURNAL Proc Natl Acad Sci U S A. 2006 Jul 18. 103(29):10961-6. PUBMED 16832055 REFERENCE 2 (bases 1 to 5666) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..5666 /organism="synthetic DNA construct" /mol_type="other DNA" enhancer 1..380 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 381..584 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 535..555 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" primer_bind 581..605 /label=LNCX /note="Human CMV promoter, forward primer" promoter 815..833 /label=SP6 promoter /note="promoter for bacteriophage SP6 RNA polymerase" primer_bind 815..832 /label=SP6 /note="SP6 promoter, forward primer" primer_bind 846..865 /label=pMT2-F /note="Synthetic intron, forward primer" CDS 920..1009 /codon_start=1 /product="three tandem HA epitope tags" /label=3xHA /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA" regulatory 1953..1962 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" polyA_signal 1966..2100 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(2003..2022) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 2057..2076 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" promoter 2169..2526 /label=SV40 promoter /note="SV40 enhancer and early promoter" primer_bind complement(2192..2212) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" rep_origin 2377..2512 /label=SV40 ori /note="SV40 origin of replication" primer_bind 2439..2458 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" primer_bind complement(2546..2563) /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind complement(2546..2562) /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind complement(2555..2577) /label=M13/pUC Forward /note="In lacZ gene" rep_origin 2775..3230 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(2862..2881) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 3072..3093 /label=F1ori-F /note="F1 origin, forward primer" primer_bind complement(3247..3266) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind 3366..3388 /label=pGEX 3' /note="pGEX vectors, reverse primer" primer_bind complement(3426..3444) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 3512..3616 /gene="bla" /label=AmpR promoter CDS 3617..4477 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(3835..3854) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 4648..5236 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 5137..5156 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 5390..5407 /label=L4440 /note="L4440 vector, forward primer" protein_bind 5524..5545 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 5560..5590 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 5598..5614 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 5603..5625 /label=M13/pUC Reverse /note="In lacZ gene" primer_bind 5622..5638 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 5622..5638 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" ORIGIN 1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc 61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca 121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga 181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc 241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct 301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat 361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc 421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt 481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa 541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc 601 agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac cgggaccgat 661 ccagcctccg cggccgggaa cggtgcattg gaacgcggat tccccgtgcc aagagtgacg 721 taagtaccgc ctatagagtc tataggccca cccccttggc ttcgttagaa cgcggctaca 781 attaatacat aaccttatgt atcatacaca tacgatttag gtgacactat agaataacat 841 ccactttgcc tttctctcca caggtgtcca ctcccaggtc caactgcacc tcggttctat 901 cgatatgggc cgcatctttt acccatacga tgttcctgac tatgcgggct atccctatga 961 cgtcccggac tatgcaggat cctatccata tgacgttcca gattacgctg ctcagcgcga 1021 attcgcggcc gcagctgcag gaggtcccgg cgcggggtct gcggccccgg tctcctccac 1081 atcctccctt cccctggctg ctctcaacat gcgagtgcgg cgccgcctgt ctctgttctt 1141 gaacgtgcgg acacaggtgg cggccgactg gaccgcgctg gcggaggaga tggactttga 1201 gtacttggag atccggcaac tggagacaca agcggacccc actggcaggc tgctggacgc 1261 ctggcaggga cgccctggcg cctctgtagg ccgactgctc gagctgctta ccaagctggg 1321 ccgcgacgac gtgctgctgg agctgggacc cagcattgag gaggattgcc aaaagtatat 1381 cttgaagcag cagcaggagg aggctgagaa gcctttacag gtggccgctg tagacagcag 1441 tgtcccacgg acagcagagc tggcgggcat caccacactt gatgaccccc tggggcatat 1501 gcctgagcgt ttcgatgcct tcatctgcta ttgccccagc gacatccagt ttgtgcagga 1561 gatgatccgg caactggaac agacaaacta tcgactgaag ttgtgtgtgt ctgaccgcga 1621 tgtcctgcct ggcacctgtg tctggtctat tgctagtgag ctcatcgaaa agaggtgccg 1681 ccggatggtg gtggttgtct ctgatgatta cctgcagagc aaggaatgtg acttccagac 1741 caaatttgca ctcagcctct ctccaggtgc ccatcagaag cgactgatcc ccatcaagta 1801 caaggcaatg aagaaagagt tccccagcat cctgaggttc atcactgtct gcgactacac 1861 caacccctgc accaaatctt ggttctggac tcgccttgcc aaggccttgt ccctgccctg 1921 agtcgactct agacccgggc tgcagaagct tggccgccat ggcccaactt gtttattgca 1981 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt 2041 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc 2101 gggaattaat tcggcgcagc accatggcct gaaataacct ctgaaagagg aacttggtta 2161 ggtaccttct gaggcggaaa gaaccagctg tggaatgtgt gtcagttagg gtgtggaaag 2221 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc 2281 aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat 2341 tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt 2401 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc 2461 gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt 2521 tgcaaaaagc tgttaacagc ttggcactgg ccgtcgtttt acaacgtcgt gactgggaaa 2581 accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta 2641 atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat 2701 ggcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatacgtc 2761 aaagcaacca tagtacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac 2821 gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc 2881 ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt 2941 agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt tgggtgatgg 3001 ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac 3061 gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta tctcgggcta 3121 ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat 3181 ttaacaaaaa tttaacgcga attttaacaa aatattaacg tttacaattt tatggtgcac 3241 tctcagtaca atctgctctg atgccgcata gttaagccag ccccgacacc cgccaacacc 3301 cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac 3361 cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg 3421 aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta 3481 gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta 3541 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata 3601 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc 3661 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga 3721 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct 3781 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg 3841 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta 3901 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat 3961 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt 4021 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga 4081 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga 4141 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga 4201 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc 4261 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc 4321 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg 4381 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat 4441 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata 4501 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct 4561 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga 4621 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg 4681 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc 4741 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct 4801 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc 4861 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt 4921 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg 4981 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct 5041 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag 5101 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag 5161 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg 5221 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg 5281 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac 5341 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt 5401 gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat 5461 tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc 5521 aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc 5581 tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacat 5641 gattacgaat taattcgagc tcgccc //