LOCUS Exported 6753 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Retroviral vector expressing NDUFAF5-HA-IRES-Bsd. ACCESSION . VERSION . KEYWORDS pMXs-NDUFAF5-HA SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 6753) AUTHORS Wang T, Yu H, Hughes NW, Liu B, Kendirli A, Klein K, Chen WW, Lander ES, Sabatini DM TITLE Gene Essentiality Profiling Reveals Gene Networks and Synthetic Lethal Interactions with Oncogenic Ras. JOURNAL Cell. 2017 Feb 1. pii: S0092-8674(17)30061-2. doi: 10.1016/j.cell.2017.01.013. PUBMED 28162770 REFERENCE 2 (bases 1 to 6753) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..6753 /organism="synthetic DNA construct" /mol_type="other DNA" misc_feature 603..960 /label=MMLV Psi /note="packaging signal of Moloney murine leukemia virus (MMLV)" CDS 1019..1435 /label=gag (truncated) /note="truncated Moloney murine leukemia virus (MMLV) gag gene lacking the start codon" primer_bind 1365..1387 /label=pLXSN 5' /note="Murine stem cell virus, forward primer. Also called MSCV" primer_bind 1403..1419 /label=pBABE 5' /note="Psi packaging signal, forward primer for pBABE vectors" misc_feature 1445..1819 /label=pol region /note="Moloney murine leukemia virus (MMLV) pol region containing the splice acceptor site" primer_bind 1757..1773 /label=pBMN 5' /note="MMLV sequence, for inserts in pBMN retroviral vector" CDS 2919..2945 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /label=HA /translation="YPYDVPDYA" CDS 2968..2991 /codon_start=1 /product="FLAG(R) epitope tag, followed by an enterokinase cleavage site" /label=FLAG /translation="DYKDDDDK" misc_feature 3030..3616 /label=IRES2 /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" primer_bind complement(3197..3214) /label=IRES reverse /note="IRES internal ribosome entry site, reverse primer. Also called pCDH-rev" primer_bind 3424..3443 /label=IRES-F /note="IRES internal ribosome entry site, forward primer" LTR 4258..4727 /note="long terminal repeat from Moloney murine leukemia virus" primer_bind complement(4857..4874) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(5028..5616) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(5108..5127) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(5780..6640) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 6403..6422 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(6641..6745) /gene="bla" /label=AmpR promoter ORIGIN 1 cccgaaaagt gccacctgca taatgaaaga ccccacctgt aggtttggca agctagctta 61 agtaacgcca ttttgcaagg catggaaaaa tacataactg agaatagaaa agttcagatc 121 aaggtcagga acagatggaa cagctgaata tgggccaaac aggatatctg tggtaagcag 181 ttcctgcccc ggctcagggc caagaacaga tggtccccag atgcggtcca gccctcagca 241 gtttctagag aaccatcaga tgtttccagg gtgccccaag gacctgaaat gaccctgtgc 301 cttatttgaa ctaaccaatc agttcgcttc tcgcttctgt tcgcgcgctt ctgctccccg 361 agctcaataa aagagcccac aacccctcac tcggcgcgcc agtcctccga ttgactgagt 421 cgcccgggta cccgtgtatc caataaaccc tcttgcagtt gcatccgact tgtggtctcg 481 ctgttccttg ggagggtctc ctctgagtga ttgactaccc gtcagcgggg gtctttcatt 541 tgggggctcg tccgggatcg ggagacccct gcccagggac caccgaccca ccaccgggag 601 gtaagctggc cagcaactta tctgtgtctg tccgattgtc tagtgtctat gactgatttt 661 atgcgcctgc gtcggtacta gttagctaac tagctctgta tctggcggac ccgtggtgga 721 actgacgagt tcggaacacc cggccgcaac cctgggagac gtcccaggga cttcgggggc 781 cgtttttgtg gcccgacctg agtccaaaaa tcccgatcgt tttggactct ttggtgcacc 841 cccctaatag gagggatatg tggttctggt aggagacgag aacctaaaac agttcccgcc 901 tccgtctgaa tttttgcttt cggtttggga ccgaagccgc gccgcgcgtc ttgtctgctg 961 cagcatcgtt ctgtgttgtc tctgtctgac tgtgtttctg tatttgtctg aaaattaggg 1021 ccagactgtt accactccct taagtttgac cttaggtcac tggaaagatg tcgagcggat 1081 cgctcacaac cagtcggtag atgtcaagaa gagacgttgg gttaccttct gctctgcaga 1141 atggccaacc tttaacgtcg gatggccgcg agacggcacc tttaaccgag acctcatcac 1201 ccaggttaag atcaaggtct tttcacctgg cccgcatgga cacccagacc aggtccccta 1261 catcgtgacc tgggaagcct tggcttttga cccccctccc tgggtcaagc cctttgtaca 1321 ccctaagcct ccgcctcctc ttcctccatc cgccccgtct ctcccccttg aacctcctcg 1381 ttcgaccccg cctcgatcct ccctttatcc agccctcact ccttctctag gcgcccccat 1441 atggccatat gagatcttat atggggcacc cccgcccctt gtaaacttcc ctgaccctga 1501 catgacaaga gttactaaca gcccctctct ccaagctcac ttacaggctc tctacttagt 1561 ccagcacgaa gtctggagac ctctggcggc agcctaccaa gaacaactgg accgaccggt 1621 ggtacctcac ccttaccgag tcggcgacac agtgtgggtc cgccgacacc agactaagaa 1681 cctagaacct cgctggaaag gaccttacac agtcctgctg accaccccca ccgccctcaa 1741 agtagacggc atcgcagctt ggatacacgc cgcccacgtg aaggctgccg accccggggg 1801 tggaccatcc tctagactgc cggatctagc tagttaatta aggatccgcc accatgctgc 1861 ggccggcagg gctctggcgc ttatgtcggc gaccttgggc ggcgagggtc ccagcggaga 1921 atcttggccg tagggaagtc acctctggtg tctctccccg cggtagcacc tcgcccagaa 1981 ccctgaatat tttcgaccgg gatttgaaaa ggaaacagaa gaactgggca gcccggcagc 2041 ccgagccgac caaatttgac tacctgaagg aggaggttgg aagtcggatc gcagaccgtg 2101 tatatgacat acccagaaat ttcccccttg ctttggatct tggttgtgga agaggttaca 2161 ttgcacaata tttgaataag gaaactattg gaaagttttt ccaagctgac attgcagaaa 2221 atgctttgaa aaattcctca gaaacagaaa tacctactgt cagcgtttta gctgatgaag 2281 aattccttcc cttcaaagaa aatacatttg acctggtggt tagcagttta agtttgcatt 2341 gggtgaatga ccttcctaga gcacttgagc agattcatta tattttaaaa ccagatggag 2401 tgtttatcgg tgcaatgttt ggaggcgaca cactctatga acttcggtgt tccttacagt 2461 tagcggaaac ggaaagggaa ggaggatttt ctccacacat ttctcctttc actgctgtca 2521 atgacctggg acatctgctt gggagagctg gctttaatac tctgactgtg gacactgatg 2581 aaattcaagt taactatcct gggatgtttg aattgatgga agatttacaa ggtatgggtg 2641 agagtaactg tgcttggaat agaaaagccc tgctgcatcg agacacaatg ctggcagctg 2701 cggcagtgta cagagaaatg tacagaaatg aagatggttc agtacctgct acataccaga 2761 tctattacat gataggatgg aaatatcatg agtcacaggc aagaccagct gaaagaggtt 2821 ccgcaactgt gtcatttgga gagctaggaa aaataaacaa ccttatgcca ccagggaaaa 2881 aatcacaagc ggccgcagga ggcggtggag gcggtggata cccctatgat gtgcctgact 2941 acgcatgatt ataaagatca tgacattgat tacaaggatg acgatgacaa gtaataataa 3001 attccgcccc cccccccccc cccccccccc ccctctccct cccccccccc taacgttact 3061 ggccgaagcc gcttggaata aggccggtgt gcgtttgtct atatgttatt ttccaccata 3121 ttgccgtctt ttggcaatgt gagggcccgg aaacctggcc ctgtcttctt gacgagcatt 3181 cctaggggtc tttcccctct cgccaaagga atgcaaggtc tgttgaatgt cgtgaaggaa 3241 gcagttcctc tggaagcttc ttgaagacaa acaacgtctg tagcgaccct ttgcaggcag 3301 cggaaccccc cacctggcga caggtgcctc tgcggccaaa agccacgtgt ataagataca 3361 cctgcaaagg cggcacaacc ccagtgccac gttgtgagtt ggatagttgt ggaaagagtc 3421 aaatggctct cctcaagcgt attcaacaag gggctgaagg atgcccagaa ggtaccccat 3481 tgtatgggat ctgatctggg gcctcggtgc acatgcttta catgtgttta gtcgaggtta 3541 aaaaaacgtc taggcccccc gaaccacggg gacgtggttt tcctttgaaa aacacgatga 3601 taatatggcc acaaccatgg ttcctttgtc tcaagaagaa tccaccctca ttgaaagagc 3661 aacggctaca atcaacagca tccccatctc tgaagactac agcgtcgcca gcgcagctct 3721 ctctagcgac ggccgcatct tcactggtgt caatgtatat cattttactg ggggaccttg 3781 tgcagaactc gtggtgctgg gcactgctgc tgctgcggca gctggcaacc tgacttgtat 3841 cgtcgcgatc ggaaatgaga acaggggcat cttgagcccc tgcggacggt gccgacaggt 3901 gcttctcgat ctgcatcctg ggatcaaagc catagtgaag gacagtgatg gacagccgac 3961 ggcagttggg attcgtgaat tgctgccctc tggttatgtg tgggagggct aagtcgacga 4021 taaaataaaa gattttattt agtctccaga aaaagggggg aatgaaagac cccacctgta 4081 ggtttggcaa gctagcttaa gtaacgccat tttgcaaggc atggaaaaat acataactga 4141 gaatagagaa gttcagatca aggtcaggaa cagatggaac agctgaatat gggccaaaca 4201 ggatatctgt ggtaagcagt tcctgccccg gctcagggcc aagaacagat ggaacagctg 4261 aatatgggcc aaacaggata tctgtggtaa gcagttcctg ccccggctca gggccaagaa 4321 cagatggaac agctgaatat gggccaaaca ggatatctgt ggtaagcagt tcctgccccg 4381 gctcagggcc aagaacagat ggtccccaga tgcggtccag ccctcagcag tttctagaga 4441 accatcagat gtttccaggg tgccccaagg acctgaaatg accctgtgcc ttatttgaac 4501 taaccaatca gttcgcttct cgcttctgtt cgcgcgcttc tgctccccga gctcaataaa 4561 agagcccaca acccctcact cggggcgcca gtcctccgat tgactgagtc gcccgggtac 4621 ccgtgtatcc aataaaccct cttgcagttg catccgactt gtggtctcgc tgttccttgg 4681 gagggtctcc tctgagtgat tgactacccg tcagcggggg tctttcacat gcagcatgta 4741 tcaaaattaa tttggttttt tttcttaagt atttacatta aatggccata gttgcattaa 4801 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg 4861 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag 4921 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa 4981 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc 5041 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca 5101 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg 5161 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct 5221 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt 5281 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag 5341 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc 5401 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac 5461 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga 5521 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc 5581 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg 5641 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca 5701 aaaaggatct tcacctagat ccttttgcgg ccggccgcaa atcaatctaa agtatatatg 5761 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct 5821 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg 5881 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc 5941 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa 6001 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc 6061 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt 6121 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc 6181 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt 6241 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc 6301 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt 6361 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata 6421 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga 6481 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag 6541 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa 6601 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt 6661 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga 6721 aaaataaaca aataggggtt ccgcgcacat ttc //