LOCUS Exported 10194 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION For expressing YFP in mammalian cells upon Cre co-expression. Can be made into Lentivirus.. ACCESSION . VERSION . KEYWORDS FU - dio YFP - W SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 10194) AUTHORS Villa KL, Berry KP, Subramanian J, Cha JW, Oh WC, Kwon HB, Kubota Y, So PT, Nedivi E TITLE Inhibitory Synapses Are Repeatedly Assembled and Removed at Persistent Sites In Vivo. JOURNAL Neuron. 2016 Feb 3. pii: S0896-6273(16)00011-8. doi: 10.1016/j.neuron.2016.01.010. PUBMED 26853302 REFERENCE 2 (bases 1 to 10194) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..10194 /organism="synthetic DNA construct" /mol_type="other DNA" protein_bind 107..128 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 143..173 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 181..197 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 186..208 /label=M13/pUC Reverse /note="In lacZ gene" primer_bind 205..221 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 205..221 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" polyA_signal 270..391 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(281..300) /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind 335..354 /label=SV40pA-R /note="SV40 polyA, reverse primer" CDS complement(521..895) /codon_start=1 /gene="Sh ble from Streptoalloteichus hindustanus" /product="antibiotic-binding protein" /label=BleoR /note="confers resistance to bleomycin, phleomycin, and Zeocin(TM)" /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR EFALRDPAGNCVHFVAEEQD" promoter complement(914..961) /label=EM7 promoter /note="synthetic bacterial promoter " promoter complement(1009..1338) /label=SV40 promoter /note="SV40 enhancer and early promoter" rep_origin 1023..1158 /label=SV40 ori /note="SV40 origin of replication" primer_bind complement(1077..1096) /label=SV40pro-F /note="SV40 promoter/origin, forward primer" primer_bind 1323..1343 /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" rep_origin complement(1352..1780) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(1462..1483) /label=F1ori-F /note="F1 origin, forward primer" primer_bind 1674..1693 /label=F1ori-R /note="F1 origin, reverse primer" polyA_signal 1826..2050 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" primer_bind 2039..2056 /label=BGH-rev /note="Bovine growth hormone terminator, reverse primer. Also called BGH reverse" LTR 2082..2262 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" primer_bind 2767..2783 /label=pBluescriptKS /note="For pBluescript vector" primer_bind 2768..2784 /label=KS primer /note="common sequencing primer, one of multiple similar variants" misc_feature 2787..3375 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" CDS 2893..2904 /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" primer_bind 3302..3322 /label=WPRE-R /note="WPRE, reverse primer" protein_bind 3400..3433 /label=loxP /bound_moiety="Cre recombinase" /note="Cre-mediated recombination occurs in the 8-bp core sequence (GCATACAT)." protein_bind 3484..3517 /label=lox2272 /bound_moiety="Cre recombinase" /note="Cre-mediated recombination occurs in the 8-bp core sequence (GGATACTT). lox2272 sites are compatible with each other, but incompatible with loxP or loxN sites (Lee and Saito, 1988)." regulatory 3524..3533 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 3530..4249 /codon_start=1 /product="enhanced YFP" /label=EYFP /note="mammalian codon-optimized" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" primer_bind complement(3575..3596) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(3836..3855) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 4183..4204 /label=EGFP-C /note="EGFP, forward primer" protein_bind complement(4258..4291) /label=loxP /bound_moiety="Cre recombinase" /note="Cre-mediated recombination occurs in the 8-bp core sequence (GCATACAT)." protein_bind complement(4342..4375) /label=lox2272 /bound_moiety="Cre recombinase" /note="Cre-mediated recombination occurs in the 8-bp core sequence (GGATACTT). lox2272 sites are compatible with each other, but incompatible with loxP or loxN sites (Lee and Saito, 1988)." promoter complement(4428..5639) /label=UbC promoter /note="human ubiquitin C promoter" primer_bind complement(4572..4591) /label=hUBCpro-F /note="Human Ubiquitin C (UbC) promoter, forward primer" misc_feature 5704..5821 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" CDS complement(6119..6163) /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" misc_feature 6348..6581 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." misc_feature 7074..7199 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" LTR 7246..7426 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" promoter complement(7444..7642) /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind complement(7469..7489) /label=CMV-F /note="Human CMV immediate early promoter, forward primer" enhancer 7644..8023 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" primer_bind 8195..8214 /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" promoter 8289..8393 /gene="bla" /label=AmpR promoter CDS 8394..9254 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(8612..8631) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 9425..10013 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 9914..9933 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 10167..10184 /label=L4440 /note="L4440 vector, forward primer" ORIGIN 1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc 61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc 121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa 181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagctct 241 agctagaggt cgacggtata cagacatgat aagatacatt gatgagtttg gacaaaccac 301 aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt 361 tgtaaccatt ataagctgca ataaacaagt tggggtgggc gaagaactcc agcatgagat 421 ccccgcgctg gaggatcatc cagccggcgt cccggaaaac gattccgaag cccaaccttt 481 catagaaggc ggcggtggaa tcgaaatctc gtagcacgtg tcagtcctgc tcctcggcca 541 cgaagtgcac gcagttgccg gccgggtcgc gcagggcgaa ctcccgcccc cacggctgct 601 cgccgatctc ggtcatggcc ggcccggagg cgtcccggaa gttcgtggac acgacctccg 661 accactcggc gtacagctcg tccaggccgc gcacccacac ccaggccagg gtgttgtccg 721 gcaccacctg gtcctggacc gcgctgatga acagggtcac gtcgtcccgg accacaccgg 781 cgaagtcgtc ctccacgaag tcccgggaga acccgagccg gtcggtccag aactcgaccg 841 ctccggcgac gtcgcgcgcg gtgagcaccg gaacggcact ggtcaacttg gccatggttt 901 agttcctcac cttgtcgtat tatactatgc cgatatacta tgccgatgat taattgtcaa 961 cacgtgctga tcagatccga aaatggatat acaagctccc gggagctttt tgcaaaagcc 1021 taggcctcca aaaaagcctc ctcactactt ctggaatagc tcagaggcag aggcggcctc 1081 ggcctctgca taaataaaaa aaattagtca gccatggggc ggagaatggg cggaactggg 1141 cggagttagg ggcgggatgg gcggagttag gggcgggact atggttgctg actaattgag 1201 atgcatgctt tgcatacttc tgcctgctgg ggagcctggg gactttccac acctggttgc 1261 tgactaattg agatgcatgc tttgcatact tctgcctgct ggggagcctg gggactttcc 1321 acaccctaac tgacacacat tccacagaat taattcgcgt taaatttttg ttaaatcagc 1381 tcatttttta accaataggc cgaaatcggc aaaatccctt ataaatcaaa agaatagacc 1441 gagatagggt tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac 1501 tccaacgtca aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca 1561 ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg 1621 agcccccgat ttagagcttg acggggaaag ccggcgaacg tggcgagaaa ggaagggaag 1681 aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc 1741 accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt ggggataccc cctagagccc 1801 cagctggttc tttccgcctc agaagccata gagcccaccg catccccagc atgcctgcta 1861 ttgtcttccc aatcctcccc cttgctgtcc tgccccaccc caccccccag aatagaatga 1921 cacctactca gacaatgcga tgcaatttcc tcattttatt aggaaaggac agtgggagtg 1981 gcaccttcca gggtcaagga aggcacgggg gaggggcaaa caacagatgg ctggcaacta 2041 gaaggcacag tcgaggctga tcagcgggtt taaacgggcc ctgctagaga ttttccacac 2101 tgactaaaag ggtctgaggg atctctagtt accagagtca cacaacagac gggcacacac 2161 tacttgaagc actcaaggca agctttattg aggcttaagc agtgggttcc ctagttagcc 2221 agagagctcc caggctcaga tctggtctaa ccagagagac ccagtacagt ccggatgcag 2281 ctctcgggcc atgtgatgaa atgctaggcg gctgtcaaac ctccactcta atacttctct 2341 ctccgggtca tccatcccat gcaggctcac agggtgtaac aagcgggtgt tctctccttc 2401 attggcttct tctaccttct cttgctcaac tggtactagc ttgtagcacc atccaaaggt 2461 cagtggatat ctgatccctg gccctggtgt gtagttctgc caatcaggga agtagccttg 2521 tgtgtggtag atccacagat caaggatatc ttgtcttcgt tgggagtgaa ttagcccttc 2581 cagtcccccc ttttctttta aaaagtggct aagatctaca gctgccttgt aagtcattgg 2641 tcttaaaggt acctgaggtg tgactggaaa acccacctcc tcctcctctt gtgcttctag 2701 ccaggcacaa tcagcattgg tagctgctgt attgctactt gtgattgctc catgtttttc 2761 taggtctcga ggtcgacggt atcgatgcgg ggaggcggcc caaagggaga tccgactcgt 2821 ctgagggcga aggcgaagac gcggaagagg ccgcagagcc ggcagcaggc cgcgggaagg 2881 aaggtccgct ggattgaggg ccgaagggac gtagcagaag gacgtcccgc gcagaatcca 2941 ggtggcaaca caggcgagca gccaaggaaa ggacgatgat ttccccgaca acaccacgga 3001 attgtcagtg cccaacagcc gagcccctgt ccagcagcgg gcaaggcagg cggcgatgag 3061 ttccgccgtg gcaataggga gggggaaagc gaaagtcccg gaaaggagct gacaggtggt 3121 ggcaatgccc caaccagtgg gggttgcgtc agcaaacaca gtgcacacca cgccacgttg 3181 cctgacaacg ggccacaact cctcataaag agacagcaac caggatttat acaaggagga 3241 gaaaatgaaa gccatacggg aagcaatagc atgatacaaa ggcattaaag cagcgtatcc 3301 acatagcgta aaaggagcaa catagttaag aataccagtc aatctttcac aaattttgta 3361 atccagaggt tgattatcga taagcttgat atcgaattca taacttcgta tagcatacat 3421 tatacgaagt tattctttgc accattctaa agaataacag tgataatttc tgggttaagg 3481 caaataactt cgtataggat actttatacg aagttatgct agcgccacca tggtgagcaa 3541 gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa 3601 cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac 3661 cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac 3721 cttcggctac ggcctgcagt gcttcgcccg ctaccccgac cacatgaagc agcacgactt 3781 cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga 3841 cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat 3901 cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta 3961 caactacaac agccacaacg tctatatcat ggccgacaag cagaagaacg gcatcaaggt 4021 gaacttcaag atccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca 4081 gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcta 4141 ccagtccgcc ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt 4201 cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaagtaag gcgcgccata 4261 acttcgtata atgtatgcta tacgaagtta ttaagaggtt tcatattgct aatagcagct 4321 acaatccagc taccattctg cataacttcg tataaagtat cctatacgaa gttattccgg 4381 agtcgactct agaggatcct ctagagtcga cctgcagccc aagcttcgtc taacaaaaaa 4441 gccaaaaacg gccagaattt agcggacaat ttactagtct aacactgaaa attacatatt 4501 gacccaaatg attacatttc aaaaggtgcc taaaaaactt cacaaaacac actcgccaac 4561 cccgagcgca tagttcaaaa ccggagcttc agctacttaa gaagataggt acataaaacc 4621 gaccaaagaa actgacgcct cacttatccc tcccctcacc agaggtccgg cgcctgtcga 4681 ttcaggagag cctaccctag gcccgaaccc tgcgtcctgc gacggagaaa agcctaccgc 4741 acacctaccg gcaggtggcc ccaccctgca ttataagcca acagaacggg tgacgtcacg 4801 acacgacgag ggcgcgcgct cccaaaggta cgggtgcact gcccaacggc accgccataa 4861 ctgccgcccc cgcaacagac gacaaaccga gttctccagt cagtgacaaa cttcacgtca 4921 gggtccccag atggtgcccc agcccatctc acccgaataa gagctttccc gcattagcga 4981 aggcctcaag accttgggtt cttgccgccc accatgcccc ccaccttgtt tcaacgacct 5041 cacagcccgc ctcacaagcg tcttccattc aagactcggg aacagccgcc attttgctgc 5101 gctcccccca acccccagtt cagggcaacc ttgctcgcgg acccagacta cagcccttgg 5161 cggtctctcc acacgcttcc gtcccaccga gcggcccggc ggccacgaaa gccccggcca 5221 gcccagcagc ccgctactca ccaagtgacg atcacagcga tccacaaaca agaaccgcga 5281 cccaaatccc ggctgcgacg gaactagctg tgccacaccc ggcgcgtcct tatataatca 5341 tcggcgttca ccgccccacg gagatccctc cgcagaatcg ccgagaaggg actacttttc 5401 ctcgcctgtt ccgctctctg gaaagaaaac cagtgcccta gagtcaccca agtcccgtcc 5461 taaaatgtcc ttctgctgat actggggttc taaggccgag tcttatgagc agcgggccgc 5521 tgtcctgagc gtccgggcgg aaggatcagg acgctcgctg cgcccttcgt ctgacgtggc 5581 agcgctcgcc gtgaggaggg gggcgcccgc gggaggcgcc aaaacccggc gcggaggcca 5641 gatctggagc cgacacgggt taattaacca aactggatct ctgctgtccc tgtaataaac 5701 ccgaaaattt tgaatttttg taatttgttt ttgtaattct ttagtttgta tgtctgttgc 5761 tattatgtct actattcttt cccctgcact gtacccccca atcccccctt ttcttttaaa 5821 attgtggatg aatactgcca tttgtctgca gaattggcgc acgcagtgcc gatccgttca 5881 ctaatcgaat ggatctgtct ctgtctctct ctccaccttc ttcttctatt ccttcgggcc 5941 tgtcgggtcc cctcggggtt gggaggtggg tctgaaacga taatggtgaa tatccctgcc 6001 taactctatt cactatagaa agtacagcaa aaactattct taaacctacc aagcctccta 6061 ctatcattat gaataatttt atataccaca gccaatttgt tatgttaaac caattccaca 6121 aacttgccca tttatctaat tccaataatt cttgttcatt cttttcttgc tggttttgcg 6181 attcttcaat taaggagtgt attaagcttg tgtaattgtt aatttctctg tcccactcca 6241 tccaggtcgt gtgattccaa atctgttcca gagatttatt actccaacta gcattccaag 6301 gcacagcagt ggtgcaaatg agttttccag agcaacccca aatccccagg agctgttgat 6361 cctttaggta tctttccaca gccaggattc ttgcctggag ctgcttgatg ccccagactg 6421 tgagttgcaa cagatgctgt tgcgcctcaa tagccctcag caaattgttc tgctgctgca 6481 ctataccaga caataattgt ctggcctgta ccgtcagcgt cattgacgct gcgcccatag 6541 tgcttcctgc tgctcccaag aacccaagga acaaagctcc tattcccact gctctttttt 6601 ctctctgcac cactcttctc tttgccttgg tgggtgctac tcctaatggt tcaattttta 6661 ctactttata tttatataat tcacttctcc aattgtccct catatctcct cctccaggtc 6721 tgaagatcag cggccgcttg ctgtgcggtg gtcttacttt tgttttgctc ttcctctatc 6781 ttgtctaaag cttccttggt gtcttttatc tctatccttt gatgcacaca atagagggtt 6841 gctactgtat tatataatga tctaagttct tctgatcctg tctgaaggga tggttgtagc 6901 tgtcccagta tttgtctaca gccttctgat gtttctaaca ggccaggatt aactgcgaat 6961 cgttctagct ccctgcttgc ccatactata tgttttaatt tatatttttt ctttccccct 7021 ggccttaacc gaattttttc ccatcgcgat ctaattctcc cccgcttaat actgacgctc 7081 tcgcacccat ctctctcctt ctagcctccg ctagtcaaaa tttttggcgt actcaccagt 7141 cgccgcccct cgcctcttgc cgtgcgcgct tcagcaagcc gagtcctgcg tcgagagagc 7201 tcctctggtt tccctttcgc tttcaagtcc ctgttcgggc gccactgcta gagattttcc 7261 acactgacta aaagggtctg agggatctct agttaccaga gtcacacaac agacgggcac 7321 acactacttg aagcactcaa ggcaagcttt attgaggctt aagcagtggg ttccctagtt 7381 agccagagag ctcccaggct cagatctggt ctaaccagag agacccagta caggcaaaac 7441 gcgctgctta tatagacctc ccaccgtaca cgcctaccgc ccatttgcgt caatggggcg 7501 gagttgttac gacattttgg aaagtcccgt tgattttggt gccaaaacaa actcccattg 7561 acgtcaatgg ggtggagact tggaaatccc cgtgagtcaa accgctatcc acgcccattg 7621 atgtactgcc aaaaccgcat caccatggta atagcgatga ctaatacgta gatgtactgc 7681 caagtaggaa agtcccataa ggtcatgtac tgggcataat gccaggcggg ccatttaccg 7741 tcattgacgt caataggggg cgtacttggc atatgataca cttgatgtac tgccaagtgg 7801 gcagtttacc gtaaatactc cacccattga cgtcaatgga aagtccctat tggcgttact 7861 atgggaacat acgtcattat tgacgtcaat gggcgggggt cgttgggcgg tcagccaggc 7921 gggccattta ccgtaagtta tgtaacgcgg aactccatat atgggctatg aactaatgac 7981 cccgtaattg attactatta ataactagtc aataatcaat gtcaacgcgt atatctggcc 8041 cgtacatcgc gaagcagcgc aaaacgccta accctaagca gattcttcat gcaattgtcg 8101 gtcaagcctt gccttgttgt agcttaaatt ttgctcgcgc actactcagc gacctccaac 8161 acacaagcag ggagcagata ctggcttaac tatgcggcat cagagcagat tgtactgaga 8221 gtgcaccata ggggatcggg agatctcccg atccgtcgac gtcaggtggc acttttcggg 8281 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc 8341 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta 8401 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg 8461 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg 8521 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac 8581 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg 8641 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt 8701 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg 8761 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac 8821 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt 8881 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag 8941 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc 9001 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc 9061 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta 9121 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg 9181 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga 9241 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac 9301 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa 9361 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat 9421 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc 9481 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg 9541 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc 9601 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg 9661 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg 9721 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa 9781 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg 9841 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga 9901 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct 9961 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca 10021 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc 10081 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg 10141 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaag //