LOCUS       Exported                9562 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Multi-gene expression system with human box C/D proteins with U14.
ACCESSION   .
VERSION     .
KEYWORDS    pQhsCDtRNA-
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9562)
  AUTHORS   Peng Y, Yu G, Tian S, Li H
  TITLE     Co-expression and co-purification of archaeal and eukaryal box C/D 
            RNPs.
  JOURNAL   PLoS One. 2014 Jul 31;9(7):e103096. doi: 
            10.1371/journal.pone.0103096. eCollection 2014.
  PUBMED    25078083
REFERENCE   2  (bases 1 to 9562)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9562
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        1..45
                     /label=T5 promoter
                     /note="bacteriophage T5 promoter for E. coli RNA 
                     polymerase, with embedded lac operator"
     protein_bind    21..37
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    53..69
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             88..99
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             1345..1365
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=7xHis
                     /translation="HHHHHHH"
     protein_bind    complement(1382..1406)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     terminator      1428..1522
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     promoter        1551..1595
                     /label=T5 promoter
                     /note="bacteriophage T5 promoter for E. coli RNA 
                     polymerase, with embedded lac operator"
     protein_bind    1571..1587
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    1603..1619
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             1638..1649
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     protein_bind    complement(2881..2905)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     terminator      2927..3021
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     promoter        3050..3094
                     /label=T5 promoter
                     /note="bacteriophage T5 promoter for E. coli RNA 
                     polymerase, with embedded lac operator"
     protein_bind    3070..3086
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    3102..3118
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             3137..3148
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     protein_bind    3164..3188
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3576..3600)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     terminator      3622..3716
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             3810..4469
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(3876..3895)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      4534..4620
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(4713..5795)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     5757..5776
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(5796..5873)
                     /gene="lacI (mutant)"
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the 
                     promoter boosts expression of the lacI gene about 10-fold."
     primer_bind     complement(5957..5979)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    6065..6205
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     6120..6139
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(6220..6237)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6391..6979)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6471..6490)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(7150..8010)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7773..7792
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8011..8115)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8183..8201
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      8442..8536
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     promoter        8565..8609
                     /label=T5 promoter
                     /note="bacteriophage T5 promoter for E. coli RNA 
                     polymerase, with embedded lac operator"
     protein_bind    8585..8601
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     protein_bind    8617..8633
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             8652..8663
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             complement(9207..9218)
                     /codon_start=1
                     /gene="cspA"
                     /product="translation enhancing element for E. coli (Qing 
                     et al., 2004)"
                     /label=TEE
                     /translation="NHKV"
     protein_bind    complement(9394..9418)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     terminator      9440..9534
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
ORIGIN
        1 tcataaaaaa tttatttgct ttgtgagcgg ataacaatta taatagattc aattgtgagc
       61 ggataacaat ttcacacaga attcattaaa gaggagaaat taactatggg atctgtgctg
      121 ttgcacgtgc tgtttgagca cgcggtcggc tacgcgctgc tggcgctgaa ggaagtggag
      181 gagatcagtc tgctgcagcc gcaggtggag gagtccgtgc tcaacctggg caaattccac
      241 agcatcgttc gtctggtggc cttttgtccc tttgcctcat cccaggttgc cttggaaaat
      301 gccaacgccg tgtctgaagg ggttgttcat gaggacctcc gcctgctctt ggagacccac
      361 ctgccgtcca aaaagaagaa agtactcttg ggagttgggg atcccaagat tggtgccgca
      421 atacaggagg agttagggta caactgccag actggaggag tcatagctga gatcctgcga
      481 ggagttcgtc tgcacttcca caatctggtg aagggtctga ccgatctgtc agcttgtaaa
      541 gcacagctgg ggctgggaca cagctattcc cgtgccaaag ttaagtttaa tgtgaaccgg
      601 gtggacaata tgatcatcca gtccattagc ctcctggacc agctggataa ggacatcaat
      661 accttctcta tgcgtgtcag ggagtggtac gggtatcact ttccggagct ggtgaagatc
      721 atcaacgaca atgccacata ctgccgtctt gcccagttta ttggaaaccg aagggaactg
      781 aatgaggaca agctggagaa gctggaggag ctgacaatgg atggggccaa ggctaaggct
      841 attctggatg cctcacggtc ctccatgggc atggacatat ctgccattga cttgataaac
      901 atcgagagct tctccagtcg tgtggtgtct ttatctgaat accgccagag cctacacact
      961 tacctgcgct ccaagatgag ccaagtagcc cccagcctgt cagccctaat tggggaagcg
     1021 gtaggtgcac gtctcatcgc acatgctggc agcctcacca acctggccaa gtatccagca
     1081 tccacagtgc agatccttgg ggctgaaaag gccctgttca gagccctgaa gacaaggggt
     1141 aacactccaa aatatggact cattttccac tccaccttca ttggccgagc agctgccaag
     1201 aacaaaggcc gcatctcccg atacctggca aacaaatgca gtattgcctc acgaatcgat
     1261 tgcttctctg aggtgcccac gagtgtattc ggggagaagc ttcgagaaca agttgaagag
     1321 cgactgtcct tctatgagac tggacatcac catcaccatc accattaggc ggccgcctag
     1381 gacccagctt tcttgtacaa agtggtaagc ttaattagct gagcttggac tcctgttgat
     1441 agatccagta atgacctcag aactccatct ggatttgttc agaacgctcg gttgccgccg
     1501 ggcgtttttt attggtgaga atccaagtaa caacaccatt tgtcgagaaa tcataaaaaa
     1561 tttatttgct ttgtgagcgg ataacaatta taatagattc aattgtgagc ggataacaat
     1621 ttcacacaga attcattaaa gaggagaaat taactatggg atccttggtg ctgtttgaaa
     1681 cgtctgtggg ttacgccatc tttaaggttc taaatgagaa gaaacttcaa gaggttgata
     1741 gtttatggaa agaatttgaa actccagaga aagcaaacaa aatagtaaag ctaaaacatt
     1801 ttgagaaatt tcaggataca gcagaagcat tagcagcatt cacagctctg atggagggca
     1861 aaatcaataa gcagctgaaa aaagttctga agaaaatagt aaaagaagcc catgaaccgc
     1921 tggcagtagc tgatgctaaa ctaggagggg tcataaagga aaagctgaat ctcagttgta
     1981 tccatagtcc tgttgttaat gaacttatga gaggaattcg ttcacaaatg gatggattaa
     2041 tccctggggt agaaccacgt gaaatggcag ctatgtgtct tggattggct cacagcctgt
     2101 ctcgatatag attgaagttt agcgctgata aagtagacac aatgattgtt caggcaattt
     2161 ccttgttaga tgacttggat aaagaactaa acaactacat tatgcgatgt agagaatggt
     2221 atggctggca tttccctgaa ttaggaaaaa ttatttcaga taatttaaca tactgcaagt
     2281 gtttacagaa agttggcgat aggaagaact atgcctctgc caagctttct gagttgctgc
     2341 cagaagaagt tgaagcagaa gtgaaagcag ctgcagagat atcaatggga acagaggttt
     2401 cagaagaaga tatttgcaat attctgcatc tttgcaccca ggtgattgaa atctctgaat
     2461 atcgaaccca gctctatgaa tatctacaaa atcgaatgat ggccattgca cccaatgtta
     2521 cagtcatggt tggggaatta gttggagcac ggcttattgc tcatgcaggt tctcttttaa
     2581 atttggccaa gcatgcagct tctaccgttc agattcttgg agctgaaaag gcacttttca
     2641 gagccctcaa atctagacgg gataccccta agtatggtct catttatcat gcttcactcg
     2701 tgggccagac aagtcccaaa cacaaaggaa agatttctcg aatgctggca gccaaaaccg
     2761 ttttggctat ccgttatgat gcttttggtg aggattcaag ttctgcaatg ggagttgaga
     2821 acagagccaa attagaggcc aggttgagaa ctttggaaga cagataggcg gccgcctagg
     2881 acccagcttt cttgtacaaa gtggtaagct taattagctg agcttggact cctgttgata
     2941 gatccagtaa tgacctcaga actccatctg gatttgttca gaacgctcgg ttgccgccgg
     3001 gcgtttttta ttggtgagaa tccaagtaac aacaccattt gtcgagaaat cataaaaaat
     3061 ttatttgctt tgtgagcgga taacaattat aatagattca attgtgagcg gataacaatt
     3121 tcacacagaa ttcattaaag aggagaaatt aactatggga tctacaagtt tgtacaaaaa
     3181 agttggcacc atgactgagg ctgatgtgaa tccaaaggcc tatccccttg ccgatgccca
     3241 cctcaccaag aagctactgg acctcgttca gcagtcatgt aactataagc agcttcggaa
     3301 aggagccaat gaggccacca aaaccctcaa caggggcatc tctgagttca tcgtgatggc
     3361 tgcagacgcc gagccactgg agatcattct gcacctgccg ctgctgtgtg aagacaagaa
     3421 tgtgccctac gtgtttgtgc gctccaagca ggccctgggg agagcctgtg gggtctccag
     3481 gcctgtcatc gcctgttctg tcaccatcaa agaaggctcg cagctgaaac agcagatcca
     3541 atccattcag cagtccattg aaaggctctt agtctaacca actttcttgt acaaagtggt
     3601 aagcttaatt agctgagctt ggactcctgt tgatagatcc agtaatgacc tcagaactcc
     3661 atctggattt gttcagaacg ctcggttgcc gccgggcgtt ttttattggt gagaatccaa
     3721 gtaacaacac catttaaatg gagtggttac aaatggagtg gttaattaag gctagcttgg
     3781 cgagattttc aggagctaag gaagctaaaa tggagaaaaa aatcactgga tataccaccg
     3841 ttgatatatc ccaatggcat cgtaaagaac attttgaggc atttcagtca gttgctcaat
     3901 gtacctataa ccagaccgtt cagctggata ttacggcctt tttaaagacc gtaaagaaaa
     3961 ataagcacaa gttttatccg gcctttattc acattcttgc ccgcctgatg aatgctcatc
     4021 cggaatttcg tatggcaatg aaagacggtg agctggtgat atgggatagt gttcaccctt
     4081 gttacaccgt tttccatgag caaactgaaa cgttttcatc gctctggagt gaataccacg
     4141 acgatttccg gcagtttcta cacatatatt cgcaagatgt ggcgtgttac ggtgaaaacc
     4201 tggcctattt ccctaaaggg tttattgaga atatgttttt cgtctcagcc aatccctggg
     4261 tgagtttcac cagttttgat ttaaacgtgg ccaatatgga caacttcttc gcccccgttt
     4321 tcaccatggg caaatattat acgcaaggcg acaaggtgct gatgccgctg gcgattcagg
     4381 ttcatcatgc cgtttgtgat ggcttccatg tcggcagaat gcttaatgaa ttacaacagt
     4441 actgcgatga gtggcagggc ggggcgtaat ttttttaagg cagttattgg tgcccttaaa
     4501 cgcctggggt aatgactctc tagcttgagg catcaaataa aacgaaaggc tcagtcgaaa
     4561 gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctcctgag taggacaaat
     4621 ccgccctcta gattacgtgc agtcgatgat aagctgtcaa acatgagaat tgtgcctaat
     4681 gagtgagcta acttacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
     4741 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
     4801 ggcgccaggg tggtttttct tttcaccagt gagacgggca acagctgatt gcccttcacc
     4861 gcctggccct gagagagttg cagcaagcgg tccacgctgg tttgccccag caggcgaaaa
     4921 tcctgtttga tggtggttaa cggcgggata taacatgagc tgtcttcggt atcgtcgtat
     4981 cccactaccg agatatccgc accaacgcgc agcccggact cggtaatggc gcgcattgcg
     5041 cccagcgcca tctgatcgtt ggcaaccagc atcgcagtgg gaacgatgcc ctcattcagc
     5101 atttgcatgg tttgttgaaa accggacatg gcactccagt cgccttcccg ttccgctatc
     5161 ggctgaattt gattgcgagt gagatattta tgccagccag ccagacgcag acgcgccgag
     5221 acagaactta atgggcccgc taacagcgcg atttgctggt gacccaatgc gaccagatgc
     5281 tccacgccca gtcgcgtacc gtcttcatgg gagaaaataa tactgttgat gggtgtctgg
     5341 tcagagacat caagaaataa cgccggaaca ttagtgcagg cagcttccac agcaatggca
     5401 tcctggtcat ccagcggata gttaatgatc agcccactga cgcgttgcgc gagaagattg
     5461 tgcaccgccg ctttacaggc ttcgacgccg cttcgttcta ccatcgacac caccacgctg
     5521 gcacccagtt gatcggcgcg agatttaatc gccgcgacaa tttgcgacgg cgcgtgcagg
     5581 gccagactgg aggtggcaac gccaatcagc aacgactgtt tgcccgccag ttgttgtgcc
     5641 acgcggttgg gaatgtaatt cagctccgcc atcgccgctt ccactttttc ccgcgttttc
     5701 gcagaaacgt ggctggcctg gttcaccacg cgggaaacgg tctgataaga gacaccggca
     5761 tactctgcga catcgtataa cgttactggt ttcacattca ccaccctgaa ttgactctct
     5821 tccgggcgct atcatgccat accgcgaaag gttttgcacc attcgatggt gtcggaattt
     5881 cgggcagcgt tgggtcctgg ccacgggtgc gcatgatcta gagctgcctc gcgcgtttcg
     5941 gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt
     6001 aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc
     6061 ggggcgcagc catgacccag tcacgtagcg atagcggagt gtatactggc ttaactatgc
     6121 ggcatcagag cagattgtac tgagagtgca ccacatgcgg tgtgaaatac cgcacagatg
     6181 cgtaaggaga aaataccgca tcaggcgctc ttccgcttcc tcgctcactg actcgctgcg
     6241 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     6301 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     6361 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     6421 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     6481 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     6541 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     6601 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     6661 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     6721 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     6781 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt
     6841 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     6901 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     6961 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     7021 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     7081 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
     7141 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
     7201 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
     7261 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
     7321 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
     7381 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
     7441 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
     7501 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
     7561 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
     7621 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
     7681 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
     7741 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
     7801 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
     7861 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
     7921 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
     7981 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
     8041 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
     8101 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat
     8161 tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc ttcacctcga
     8221 gcttaattaa gtcgccccat caaaaaaata ttctcaacat aaaaaacttt gtgtaatact
     8281 tgtaacgctg aattctcact gtgatgatgg ttttccaaca ttcgcagttt ccaccagaaa
     8341 ggttttcctt agtgttgggt aaaccttcct tggatgtctg agtgaggatc catccttagc
     8401 gaaagctaag gatttttttt aagcttaatt agctgagctt ggactcctgt tgatagatcc
     8461 agtaatgacc tcagaactcc atctggattt gttcagaacg ctcggttgcc gccgggcgtt
     8521 ttttattggt gagaatccaa gtaacaacac catttgtcga gaaatcataa aaaatttatt
     8581 tgctttgtga gcggataaca attataatag attcaattgt gagcggataa caatttcaca
     8641 cagaattcat taaagaggag aaattaacta tgggatccgg gaagaatgtg atggtggagc
     8701 cgcatcggca tgagggtgtc ttcatttgtc gaggaaagga agatgcactg gtcaccaaga
     8761 acctggtccc tggggaatca gtttatggag agaagagagt ctcgatttcg gaaggagatg
     8821 acaaaattga gtaccgagcc tggaacccct tccgctccaa gctagcagca gcaatcctgg
     8881 gtggtgtgga ccagatccac atcaaaccgg gggctaaggt tctctacctc ggggctgcct
     8941 cgggcaccac ggtctcccat gtctctgaca tcgttggtcc ggatggtcta gtctatgcag
     9001 tcgagttctc ccaccgctct ggccgtgacc tcattaactt ggccaagaag aggaccaaca
     9061 tcattcctgt gatcgaggat gctcgacacc cacacaaata ccgcatgctc atcgcaatgg
     9121 tggatgtgat ctttgctgat gtggcccagc cagaccagac ccggattgtg gccctgaatg
     9181 cccacacctt cctgcgtaat ggaggacact ttgtgatttc cattaaggcc aactgcattg
     9241 actccacagc ctcagccgag gccgtgtttg cctccgaagt gaaaaagatg caacaggaga
     9301 acatgaagcc gcaggagcag ttgacccttg agccatatga aagagaccat gccgtggtcg
     9361 tgggagtgta caggccatag gcggccgcct aggacccagc tttcttgtac aaagtggtaa
     9421 gcttaattag ctgagcttgg actcctgttg atagatccag taatgacctc agaactccat
     9481 ctggatttgt tcagaacgct cggttgccgc cgggcgtttt ttattggtga gaatccaagt
     9541 aacaacacca tttgtcgaga aa
//
