LOCUS Exported 4303 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Secreted Luciferase reporter for PPARgamma activity. ACCESSION . VERSION . KEYWORDS PPRE-pNL1.3 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4303) AUTHORS Degrelle SA, Shoaito H, Fournier T TITLE New Transcriptional Reporters to Quantify and Monitor PPARgamma Activity. JOURNAL PPAR Res. 2017;2017:6139107. doi: 10.1155/2017/6139107. Epub 2017 Nov 1. PUBMED 29225614 REFERENCE 2 (bases 1 to 4303) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..4303 /organism="synthetic DNA construct" /mol_type="other DNA" CDS 1253..1765 /codon_start=1 /product="NanoLuc(R) luciferase" /label=Nluc /note="human codon-optimized" /translation="VFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQRI VLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGVT PNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGWR LCERILA" polyA_signal 1814..1935 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(1825..1844) /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind 1879..1898 /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind complement(2183..2200) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(2354..2942) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(2434..2453) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(3142..4002) /codon_start=1 /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" polyA_signal 4107..4155 /note="synthetic polyadenylation signal" misc_feature 4169..4260 /label=pause site /note="RNA polymerase II transcriptional pause signal from the human alpha-2 globin gene" primer_bind 4209..4228 /label=RVprimer3 /note="pGL3 vector, forward primer" ORIGIN 1 cagagccggg ccactttctc aggcctctga tttcatactg tggtgttagt tacttctgag 61 aggacagctt gctgccagag ctctattttt tatgttagag gctccttctg cctgcagact 121 ctgctgtctg ggaagggcac agcgttagga gggagaggga ggtgtgagtc cctccgtgga 181 cccgctgctt tgtacttctc tatctcattt ccttttcagc accactctgg gaaatcagta 241 ttccagcccc attttatcct cagaaaattg aggctctgag atgttatctc tgtgacctgg 301 gtcctattac gtgccaaagg catcatttaa gcctaagatg tcctggctcc aaggtgtcag 361 catctggaag acaggcgccc tcatcctgcc atccctgctg cggcttcact gtgggcccag 421 gggacatctc agccccgaga agggtcagcg gcccctcctg gaccaccgac tccccgcaga 481 actcctctgt gccctctcct caccagacct tgttcctccc agttgctccc acagccaggg 541 ggcagtgagg gctgctcttc ccccagcccc actgaggaac ccaggaaggt gaacgagaga 601 atcagtcctg gtgggggctg gggagggccc cagacatgag accagctcct cccccagggg 661 atgttatcag tgggtccaga gggcaaaata gggagcctgg tggagggagg ggcctgaaat 721 tccagtgtga aaggctgaga tgggcccgag gcccctggcc tatgtccaag ccatttcccc 781 tctcaccagc ctctccctgg ggagccagtc agctaggaag gaatgagggc tccccaggcc 841 caccccagtt cctgagctca tctgggctgc agggctggcg ggacagcagc gtggactcag 901 tctcctaggg atttcccaac tctcccgccc gcttgctgca tctggacacc ctgcctcagg 961 ccctcatctc cactggtcag caggtgacct ttgcccagcg ccctgggtcc tcagtgcctg 1021 ctgccctgga gatgatataa aacaggtcag aaccctcctg cctgtctgct cagttcatcc 1081 ctagaggcag gactctagag gatctgcgat cagatctggc ctcggcggcc aagcttggca 1141 atccggtact gttggtaaag ccaccatgaa ctccttctcc acaagcgcct tcggtccagt 1201 tgccttctcc ctgggcctgc tcctggtgtt gcctgctgcc ttccctgccc cagtcttcac 1261 actcgaagat ttcgttgggg actggcgaca gacagccggc tacaacctgg accaagtcct 1321 tgaacaggga ggtgtgtcca gtttgtttca gaatctcggg gtgtccgtaa ctccgatcca 1381 aaggattgtc ctgagcggtg aaaatgggct gaagatcgac atccatgtca tcatcccgta 1441 tgaaggtctg agcggcgacc aaatgggcca gatcgaaaaa atttttaagg tggtgtaccc 1501 tgtggatgat catcacttta aggtgatcct gcactatggc acactggtaa tcgacggggt 1561 tacgccgaac atgatcgact atttcggacg gccgtatgaa ggcatcgccg tgttcgacgg 1621 caaaaagatc actgtaacag ggaccctgtg gaacggcaac aaaattatcg acgagcgcct 1681 gatcaacccc gacggctccc tgctgttccg agtaaccatc aacggagtga ccggctggcg 1741 gctgtgcgaa cgcattctgg cgtaaggccg cgactctaga gtcggggcgg ccggccgctt 1801 cgagcagaca tgataagata cattgatgag tttggacaaa ccacaactag aatgcagtga 1861 aaaaaatgct ttatttgtga aatttgtgat gctattgctt tatttgtaac cattataagc 1921 tgcaataaac aagttaacaa caacaattgc attcatttta tgtttcaggt tcagggggag 1981 gtgtgggagg ttttttaaag caagtaaaac ctctacaaat gtggtaaaat cgataaggat 2041 ccgtcgaccg atgcccttga gagccttcaa cccagtcagc tccttccggt gggcgcgggg 2101 catgactatc gtcgccgcac ttatgactgt cttctttatc atgcaactcg taggacaggt 2161 gccggcagcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc 2221 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata 2281 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg 2341 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct 2401 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa 2461 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc 2521 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt 2581 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg 2641 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg 2701 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct 2761 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc 2821 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg 2881 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc 2941 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt 3001 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa 3061 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agcggccgca 3121 aatgctaaac cactgcagtg gttaccagtg cttgatcagt gaggcaccga tctcagcgat 3181 ctgcctattt cgttcgtcca tagtggcctg actccccgtc gtgtagatca ctacgattcg 3241 tgagggctta ccatcaggcc ccagcgcagc aatgatgccg cgagagccgc gttcaccggc 3301 ccccgatttg tcagcaatga accagccagc agggagggcc gagcgaagaa gtggtcctgc 3361 tactttgtcc gcctccatcc agtctatgag ctgctgtcgt gatgctagag taagaagttc 3421 gccagtgagt agtttccgaa gagttgtggc cattgctact ggcatcgtgg tatcacgctc 3481 gtcgttcggt atggcttcgt tcaactctgg ttcccagcgg tcaagccggg tcacatgatc 3541 acccatatta tgaagaaatg cagtcagctc cttagggcct ccgatcgttg tcagaagtaa 3601 gttggccgcg gtgttgtcgc tcatggtaat ggcagcacta cacaattctc ttaccgtcat 3661 gccatccgta agatgctttt ccgtgaccgg cgagtactca accaagtcgt tttgtgagta 3721 gtgtatacgg cgaccaagct gctcttgccc ggcgtctata cgggacaaca ccgcgccaca 3781 tagcagtact ttgaaagtgc tcatcatcgg gaatcgttct tcggggcgga aagactcaag 3841 gatcttgccg ctattgagat ccagttcgat atagcccact cttgcaccca gttgatcttc 3901 agcatctttt actttcacca gcgtttcggg gtgtgcaaaa acaggcaagc aaaatgccgc 3961 aaagaaggga atgagtgcga cacgaaaatg ttggatgctc atactcgtcc tttttcaata 4021 ttattgaagc atttatcagg gttactagta cgtctctcaa ggataagtaa gtaatattaa 4081 ggtacgggag gtattggaca ggccgcaata aaatatcttt attttcatta catctgtgtg 4141 ttggtttttt gtgtgaatcg atagtactaa catacgctct ccatcaaaac aaaacgaaac 4201 aaaacaaact agcaaaatag gctgtcccca gtgcaagtgc aggtgccaga acatttctct 4261 ggcctaactg gccggtacca tgcctgcagg tcaattctga ggg //