LOCUS Exported 9952 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Localization: Focal Adhesions, Excitation: 604, Emission: 659. ACCESSION . VERSION . KEYWORDS mCardinal-Tensin1-N-18 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 9952) AUTHORS Chu J, Haynes RD, Corbel SY, Li P, Gonzalez-Gonzalez E, Burg JS, Ataie NJ, Lam AJ, Cranfill PJ, Baird MA, Davidson MW, Ng HL, Garcia KC, Contag CH, Shen K, Blau HM, Lin MZ TITLE Non-invasive intravital imaging of cellular differentiation with a bright red-excitable fluorescent protein. JOURNAL Nat Methods. 2014 Mar 16. doi: 10.1038/nmeth.2888. PUBMED 24633408 REFERENCE 2 (bases 1 to 9952) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..9952 /organism="synthetic DNA construct" /mol_type="other DNA" promoter 1..204 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 155..175 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" regulatory 5514..5523 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 5520..6254 /codon_start=1 /product="far-red fluorescent protein derived from mNeptune, with further red-shifted spectra" /label=mCardinal /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTTEGEGKPYEGTQTQ RIKVVEGGPLPFAFDILATCFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG VLTVTQDTSLQDGCLIYNVKLRGVNFPSNGPVMQKKTLGWEATTETLYPADGGLEGRCD MALKLVGGGHLHCNLKTTYRSKKPAKNLKMPGVYFVDRRLERIKEADNETYVEQHEVAV ARYCDLPSKLGHKLNGMDELYK" polyA_signal 6376..6497 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(6413..6432) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 6467..6486 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" rep_origin complement(6504..6959) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(6641..6662) /label=F1ori-F /note="F1 origin, forward primer" primer_bind 6853..6872 /label=F1ori-R /note="F1 origin, reverse primer" promoter 6986..7090 /gene="bla" /label=AmpR promoter promoter 7092..7449 /label=SV40 promoter /note="SV40 enhancer and early promoter" primer_bind complement(7115..7135) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" rep_origin 7300..7435 /label=SV40 ori /note="SV40 origin of replication" primer_bind 7362..7381 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" CDS 7484..8278 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" primer_bind complement(7538..7557) /label=Neo-R /note="Neomycin resistance gene, reverse primer" primer_bind 8148..8167 /label=Neo-F /note="Neomycin resistance gene, forward primer" primer_bind complement(8466..8485) /label=TK-pA-R /note="Thymidine kinase polyA, reverse primer" polyA_signal 8510..8557 /label=HSV TK poly(A) signal /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" rep_origin 8886..9474 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 9375..9394 /label=pBR322ori-F /note="pBR322 origin, forward primer" enhancer 9649..9952 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" ORIGIN 1 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt 61 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac 121 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg 181 tgggaggtct atataagcag agctggttta gtgaaccgtc agatccgcta gcgctaccgg 241 actcagatct cgaggccacc atgagtgtga gccggaccat ggaggacagc tgtgagctgg 301 acctggtgta cgtcacagag aggatcatcg ctgtctcctt ccccagcaca gccaatgagg 361 agaactttcg gagcaacctc cgtgaggtgg cgcagatgct caagtccaaa catggaggca 421 actacctgct gttcaacctc tctgagcgga gacctgacat cacgaagctc catgccaagg 481 tactggaatt tggctggccc gacctccaca ccccagccct ggagaagata tgcagcatct 541 gtaaggccat ggacacatgg ctcaatgcag accctcacaa tgtcgttgtt ctacacaaca 601 agggaaaccg aggcaggata ggagttgtca tcgcggctta catgcactac agcaacattt 661 ctgccagtgc ggaccaggct ctggatcggt ttgcaatgaa gcggttctat gaggataaga 721 ttgtgcccat tggccagcca tcccaaagaa ggtacgtgca ttacttcagt ggcctgctct 781 ccggctccat caaaatgaac aacaagccct tgtttctgca ccacgtgatc atgcacggca 841 tccccaactt tgagtctaaa ggaggatgtc ggccatttct ccgcatctac caggccatgc 901 aacctgtgta cacatctggc atctacaaca tcccaggaga cagccagact agcgtctgca 961 tcaccatcga gccaggactg ctcttgaagg gagacatctt gctgaagtgc taccacaaga 1021 agttccgaag cccagcccga gacgtcatct tccgtgtgca gttccacacc tgtgccatcc 1081 atgacctggg ggttgtcttt gggaaggagg accttgatga tgctttcaaa gatgatcgat 1141 ttccagagta tggcaaagtg gagtttgtat tttcttatgg gccagagaaa attcaaggca 1201 tggagcacct ggagaacggg ccgagcgtgt ctgtggacta taacacctcc gaccccctca 1261 tccgctggga ctcctacgac aacttcagtg ggcatcgaga tgacggcatg gaggaggtgg 1321 tgggacacac gcaggggcca ctagatggga gcctgtatgc taaggtgaag aagaaagact 1381 ccctgcacgg cagcaccggg gctgttaatg ccacacgtcc tacactgtcg gccaccccca 1441 accacgtgga acacacgctt tctgtgagca gcgactcggg caactccaca gcctccacca 1501 agaccgacaa gaccgacgag cctgtccccg gggcctccag tgccactgct gccttgagtc 1561 cccaggagaa gcgggagctg gaccgcctgc tgagtggctt tggcttagag cgagagaagc 1621 aaggcgccat gtaccacacc cagcacctca ggtcccgccc agcagggggc tcggctgtgc 1681 cctcctctgg acgccacgtt gtcccagccc aggttcatgt caatggtggg gcgttagcat 1741 ctgagcggga gacagacatc ctggacgatg aattgccaaa ccaggatggt cacagtgcgg 1801 gcagcatggg cacactctct tctctggacg gggtcaccaa caccagtgag gggggctacc 1861 cagaggccct gtccccactg accaacggtc tggacaagtc ctaccccatg gagcctatgg 1921 tcaatggagg aggctacccc tacgagtctg ccagccgggc ggggcctgcc catgctggcc 1981 acacggcccc catgcggccc tcctactctg cacaggaggg tttagctggc taccagaggg 2041 aggggcccca cccagcctgg ccacagccag tgaccacctc ccactatgcc catgacccca 2101 gcggtatgtt ccgctctcaa tccttttcgg aagctgaacc ccagctgccc ccagctccgg 2161 tccgaggggg aagcagccgg gaggctgtgc aaaggggact gaactcgtgg cagcagcagc 2221 agcagcagca gcagcagcct cgcccacctc cacgccagca ggaaagagcc cacttggaga 2281 gtcttgtagc cagcaggccc agccctcagc cattggcaga gacccccatc cccagtctcc 2341 ctgagttccc gcgagcagcc tcccagcagg agattgaaca gtccatcgaa acactcaata 2401 tgctgatgct ggacctggag ccagcctccg ctgctgcccc actacacaag tcccagagtg 2461 tccccggggc ctggccaggg gcttctccac tctcctccca gcccctctct gggtcctccc 2521 gtcagtccca tccactgacc cagtccagat atggctatat ccccagtggg cattcgttgg 2581 gaacccctga gccagcccca cgggcctctc tggagtctgt ccctcctggc aggtcttact 2641 caccttatga ctatcagcca tgtttggctg ggcctaacca ggatttccat tcaaagagcc 2701 cagcctcttc ctccttgcct gccttccttc cgaccaccca cagccctcca gggcctcagc 2761 aacccccagc ctctctccct ggcctcactg ctcagcctct gctctcacca aaggaagcga 2821 cttcagaccc ctcccggact ccagaggagg agccattgaa tttagaaggg ctggtggccc 2881 acagggtagc aggggtacag gctcgggaga agcagcctgc agagccccca gcccctctgc 2941 ggaggcgggc ggccagtgat ggacagtatg agaaccagtc tccagaagcc acatcccctc 3001 gtagccctgg ggttcgctcc cctgtccagt gtgtctcccc ggagctggct cttaccatcg 3061 ctctcaatcc tggagggcgg cccaaagagc cccatttgca cagctacaag gaggccttcg 3121 aggagatgga gggaacctcc ccgagcagcc caccacccag tggggtgcgg tcccccccgg 3181 gtctggccaa gacacccctg tctgctctgg gcctgaaacc tcacaaccca gcggacatcc 3241 tgttgcaccc cacaggagtt accagaagac ggattcagcc agaggaagat gaggggaagg 3301 tggtggtcag gctgtcagaa gagccccgga gctatgtgga gtctgtggca cggacagcgg 3361 tggctggacc ccgagctcag gactctgagc ccaagagctt tagtgctcca gccacccagg 3421 cctatggcca tgagataccc ctgaggaacg ggaccctggg tggctccttt gtctccccca 3481 gccccctctc caccagcagc cccatcctca gtgctgacag cacttcagtg gggagtttcc 3541 cgtcgggaga gagcagtgac cagggtcccc ggacgcccac ccagcctctg ttggagtctg 3601 gcttccgctc aggcagcctg ggacagccca gcccgtctgc ccagagaaac taccagagct 3661 cttctcctct cccgactgtg ggcagtagct acagcagccc cgactactca cttcagcatt 3721 tcagctcctc tccagaaagc caggctcgcg ctcagttcag tgtggctggc gtccacacgg 3781 tgcctgggag ccctcaggcg cgccacagaa cagtgggcac caacactccc cctagtcctg 3841 gcttcggctg gcgggccatc aatcccagca tggctgcccc cagcagtccc agtttgagcc 3901 atcaccagat gatgggtcca ccaggcactg gcttccatgg tagcactgtc tccagccccc 3961 agagcagtgc agcgaccacc ccggggagcc ccagcctgtg tcggcaccca gcaggggtct 4021 accaggtttc tggcctccac aacaaagtgg ccaccacccc ggggagtccc agcctgggcc 4081 ggcaccctgg ggctcaccaa ggcaacctgg cctccggtct tcatagcaat gcaatagcca 4141 gccctggaag ccccagcctg ggccgtcacc tcggagggtc tggatctgtg gttcccggca 4201 gcccctgctt ggaccggcat gtggcctatg gtggctattc taccccggag gatcggagac 4261 ccacactgtc ccggcagagc agtgcctctg gctaccaggc tccttccacg ccctccttcc 4321 ctgtctcccc tgcctactac cctggcctga gcagccctgc cacctccccg tcaccagact 4381 ccgcagcctt ccggcaaggg agcccaacac cagccttgcc agagaagcga aggatgtcag 4441 tgggagaccg ggcaggcagc ctccccaact atgccaccat caatgggaag gtgtcttcgc 4501 ctgtcgccag cggcatgtcc agtcccagtg ggggcagcac cgtctccttc tcccacactc 4561 tgcccgactt ctccaagtac tccatgccag acaacagccc ggagacgcgg gctaaagtga 4621 agtttgtcca ggacacttct aagtattggt acaagcctga gatttccagg gagcaggcca 4681 tcgcgctcct caaggaccag gagccggggg ccttcatcat ccgcgacagt cactccttcc 4741 gaggcgcgta cgggctggcc atgaaggtgt cttcgccacc tccaaccatc atgcagcaga 4801 ataaaaaagg agacatgacc catgagctgg tcaggcattt tctgatagag actggcccca 4861 gaggagtcaa gctcaagggc tgccccaatg agccaaactt cggatcgctg tctgccctgg 4921 tctaccagca ctccatcatc ccattggccc tgccttgcaa gctggtcatt ccaaaccgag 4981 accccacaga tgaatcgaaa gatagctccg gccctgccaa ctcaactgca gacctgctga 5041 aacaaggggc agcctgcaat gtgctcttcg tcaactctgt ggacatggag tcactcactg 5101 ggccacaggc catctctaaa gccacatctg agacgttggc tgcagacccc acgccagctg 5161 ccaccatcgt tcacttcaaa gtctctgccc agggaatcac tctgactgac aaccagagaa 5221 agctcttttt cagacgccac taccctctca acactgtcac cttctgtgac ctggacccac 5281 aggaaagaaa gtggatgaaa acagagggtg gtgcccctgc taagctcttc ggcttcgtgg 5341 cccggaagca gggcagcacc acggacaacg cctgccacct ctttgctgag cttgacccca 5401 accagccggc ctctgccatc gtcaacttcg tctccaaggt catgctgaat gccggccaaa 5461 agagaggtag cgcatccggg ggtgcgtcag gcggcagcgg atctggaccg gtcgccacca 5521 tggtgagcaa gggcgaggag ctgatcaagg agaacatgca catgaagctg tacatggaag 5581 gcaccgtgaa caaccaccac ttcaagtgca ccaccgaagg ggagggcaag ccctacgagg 5641 gcacccagac ccagaggatt aaggtggtgg agggaggccc cctgccgttc gcattcgaca 5701 tcctggccac ctgctttatg tacgggagca agaccttcat caaccacacc cagggcatcc 5761 ccgatttctt taagcagtcc ttccctgagg gcttcacatg ggagagagtc accacatacg 5821 aagacggggg cgtgcttacc gttacccagg acaccagcct ccaggacggc tgcttgatct 5881 acaacgtcaa gctcagaggg gtgaacttcc catccaacgg ccctgtgatg cagaagaaaa 5941 cactcggctg ggaggccacc accgagaccc tgtaccccgc tgacggcggc ctggaaggca 6001 gatgcgacat ggccctgaag ctcgtgggcg ggggccacct gcactgcaac ctgaagacca 6061 catacagatc caagaaaccc gctaagaacc tcaagatgcc cggcgtctac tttgtggacc 6121 gcagactgga aagaatcaag gaggccgaca atgagaccta cgtcgagcag cacgaggtgg 6181 ctgtggccag atactgcgac ctccctagca aactggggca caaacttaat ggcatggacg 6241 agctgtacaa gtaagcggcc gcgactctag atcataatca gccataccac atttgtagag 6301 gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat 6361 gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata aagcaatagc 6421 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa 6481 ctcatcaatg tatcttaagg cgtaaattgt aagcgttaat attttgttaa aattcgcgtt 6541 aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta 6601 taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc 6661 actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg 6721 cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact 6781 aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt 6841 ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc 6901 ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc 6961 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca 7021 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa 7081 aaggaagagt cctgaggcgg aaagaaccag ctgtggaatg tgtgtcagtt agggtgtgga 7141 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca 7201 accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc 7261 aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc 7321 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag 7381 gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc 7441 ttttgcaaag atcgatcaag agacaggatg aggatcgttt cgcatgattg aacaagatgg 7501 attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca 7561 acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt 7621 tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaagacg aggcagcgcg 7681 gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga 7741 agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca 7801 ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct 7861 tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac 7921 tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc 7981 gccagccgaa ctgttcgcca ggctcaaggc gagcatgccc gacggcgagg atctcgtcgt 8041 gacccatggc gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt 8101 catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg 8161 tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat 8221 cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc 8281 gggactctgg ggttcgaaat gaccgaccaa gcgacgccca acctgccatc acgagatttc 8341 gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc 8401 tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc tagggggagg 8461 ctaactgaaa cacggaagga gacaataccg gaaggaaccc gcgctatgac ggcaataaaa 8521 agacagaata aaacgcacgg tgttgggtcg tttgttcata aacgcggggt tcggtcccag 8581 ggctggcact ctgtcgatac cccaccgaga ccccattggg gccaatacgc ccgcgtttct 8641 tccttttccc caccccaccc cccaagttcg ggtgaaggcc cagggctcgc agccaacgtc 8701 ggggcggcag gccctgccat agcctcaggt tactcatata tactttagat tgatttaaaa 8761 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa 8821 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga 8881 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg 8941 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact 9001 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac 9061 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg 9121 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg 9181 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga 9241 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc 9301 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg 9361 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc 9421 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc 9481 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt 9541 cctgcgttat cccctgattc tgtggataac cgtattaccg ccatgcatta gttattaata 9601 gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact 9661 tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat 9721 gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta 9781 tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc 9841 tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg 9901 ggactttcct acttggcagt acatctacgt attagtcatc gctattacca tg //