LOCUS Exported 12684 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Replicating base vector for constructing cpf1/CRISPR editing plasmids. ACCESSION . VERSION . KEYWORDS pSL2680 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 12684) AUTHORS Ungerer J, Pakrasi HB TITLE Cpf1 Is A Versatile Tool for CRISPR Genome Editing Across Diverse Species of Cyanobacteria. JOURNAL Sci Rep. 2016 Dec 21;6:39681. doi: 10.1038/srep39681. PUBMED 28000776 REFERENCE 2 (bases 1 to 12684) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..12684 /organism="synthetic DNA construct" /mol_type="other DNA" rep_origin 496..890 /label=RSF1010 oriV /note="replication origin of the broad-host-range plasmid RSF1010; requires the RSF1010 RepA/B/C proteins for replication (Scholz et al., 1989)" oriT 1232..1319 /label=RSF1010 oriT /note="origin of transfer of the broad-host-range plasmid RSF1010 (Scholz et al., 1989)" CDS 2558..3529 /codon_start=1 /gene="repB" /product="replication protein B of the broad-host-range plasmid RSF1010 (Scholz et al., 1989)" /label=RSF1010 RepB /translation="MKNDRTLQAIGRQLKAMGCERFDIGVRDATTGQMMNREWSAAEVL QNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDLSEFDLDDMKAEGREPALVVETSPKN YQAWVKVADAAGGELRGQIARTLASEYDADPASADSRHYGRLAGFTNRKDKHTTRAGYQ PWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQEKARRLASLELPERQLSRHRRTALD EYRSEMAGLVKRFGDDLSKCDFIAAQKLASRGRSAEEIGKAMAEASPALAERKPGHEAD YIERTVSKVMGLPSVQLARAELARAPAPRQRGMDRGGPDFSM" CDS 4040..4879 /codon_start=1 /gene="repA" /product="replication protein A of the broad-host-range plasmid RSF1010 (Scholz et al., 1989)" /label=RSF1010 RepA /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVAD GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA" primer_bind 6227..6246 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter 6350..6379 /label=lac promoter /note="promoter for the E. coli lac operon" promoter 10303..10337 /label=J23119 promoter /note="bacterial promoter (Registry of Standard Biological Parts BBa_J23119)" CDS complement(10405..10701) /codon_start=1 /gene="lacZ fragment" /product="LacZ-alpha fragment of beta-galactosidase" /label=lacZ-alpha /translation="MTMITPSAQLTLTKGNKSWRTSRGGPVPNSPYSESYYARSLAVVL QRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWDAPCSG" primer_bind 10551..10573 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 10565..10582 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 10566..10582 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind 10592..10611 /label=T7 /note="T7 promoter, forward primer" promoter 10592..10610 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(10658..10678) /label=T3 /note="T3 promoter, forward primer" promoter complement(10658..10676) /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" primer_bind complement(10697..10713) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(10697..10713) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(10710..10732) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 10721..10737 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(10745..10775) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 10790..10811 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." CDS complement(11507..12322) /codon_start=1 /gene="aph(3')-Ia" /product="aminoglycoside phosphotransferase" /label=KanR /note="confers resistance to kanamycin in bacteria or G418 (Geneticin(R)) in eukaryotes" /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" primer_bind 12230..12249 /label=Kan-R /note="Kanamycin resistance gene, reverse primer" primer_bind 12314..12333 /label=pENTR-R /note="pENTR vectors, reverse primer" ORIGIN 1 tcattaaacg cgtattcagg ctgaccctgc gcgctgcgca gggctttatt gattccattt 61 ttacactgat gaatgttccg ttgcgctgcc cggattacag ctgaaagcga ccaggtgctc 121 ggcgtggcaa gactcgcagc gaacccgtag aaagccatgc tccagccgcc cgcattggag 181 aaattcttca aattcccgtt gcacatagcc cggcaattcc tttccctgct ctgccataag 241 cgcagcgaat gccgggtaat actcgtcaac gatctgatag agaagggttt gctcgggtcg 301 gtggctctgg taacgaccag tatcccgatc ccggctggcc gtcctggccg ccacatgagg 361 catgttccgc gtccttgcaa tactgtgttt acatacagtc tatcgcttag cggaaagttc 421 ttttaccctc agccgaaatg cctgccgttg ctagacattg ccagccagtg cccgtcactc 481 ccgtactaac tgtcacgaac ccctgcaata actgtcacgc ccccctgcaa taactgtcac 541 gaacccctgc aataactgtc acgcccccaa acctgcaaac ccagcagggg cgggggctgg 601 cggggtgttg gaaaaatcca tccatgatta tctaagaata atccactagg cgcggttatc 661 agcgcccttg tggggcgctg ctgcccttgc ccaatatgcc cggccagagg ccggatagct 721 ggtctattcg ctgcgctagg ctacacaccg ccccaccgct gcgcggcagg gggaaaggcg 781 ggcaaagccc gctaaacccc acaccaaacc ccgcagaaat acgctggagc gcttttagcc 841 gctttagcgg cctttccccc tacccgaagg gtgggggcgc gtgtgcagcc ccgcagggcc 901 tgtctcggtc gatcattcag cccggctcat ccttctggcg tggcggcaga ccgaacaagg 961 cgcggtcgtg gtcgcgttca aggtacgcat ccattgccgc catgagccga tcctccggcc 1021 actcgctgct gttcaccttg gccaaaatca tggcccccac cagcaccttg cgccttgttt 1081 cgttcttgcg ctcttgctgc tgttcccttg cccgcacccg ctgaatttcg gcattgattc 1141 gcgctcgttg ttcttcgagc ttggccagcc gatccgccgc cttgttgctc cccttaacca 1201 tcttgacacc ccattgttaa tgtgctgtct cgtaggctat catggaggca cagcggcggc 1261 aatcccgacc ctactttgta ggggagggcg cacttaccgg tttctcttcg agaaactggc 1321 ctaacggcca cccttcgggc ggtgcgctct ccgagggcca ttgcatggag ccgaaaagca 1381 aaagcaacag cgaggcagca tggcgattta tcaccttacg gcgaaaaccg gcagcaggtc 1441 gggcggccaa tcggccaggg ccaaggccga ctacatccag cgcgaaggca agtatgcccg 1501 cgacatggat gaagtcttgc acgccgaatc cgggcacatg ccggagttcg tcgagcggcc 1561 cgccgactac tgggatgctg ccgacctgta tgaacgcgcc aatgggcggc tgttcaagga 1621 ggtcgaattt gccctgccgg tcgagctgac cctcgaccag cagaaggcgc tggcgtccga 1681 gttcgcccag cacctgaccg gtgccgagcg cctgccgtat acgctggcca tccatgccgg 1741 tggcggcgag aacccgcact gccacctgat gatctccgag cggatcaatg acggcatcga 1801 gcggcccgcc gctcagtggt tcaagcggta caacggcaag accccggaga agggcggggc 1861 acagaagacc gaagcgctca agcccaaggc atggcttgag cagacccgcg aggcatgggc 1921 cgaccatgcc aaccgggcat tagagcgggc tggccacgac gcccgcattg accacagaac 1981 acttgaggcg cagggcatcg agcgcctgcc cggtgttcac ctggggccga acgtggtgga 2041 gatggaaggc cggggcatcc gcaccgaccg ggcagacgtg gccctgaaca tcgacaccgc 2101 caacgcccag atcatcgact tacaggaata ccgggaggca atagaccatg aacgcaatcg 2161 acagagtgaa gaaatccaga ggcatcaacg agttagcgga gcagatcgaa ccgctggccc 2221 agagcatggc gacactggcc gacgaagccc ggcaggtcat gagccagacc cagcaggcca 2281 gcgaggcgca ggcggcggag tggctgaaag cccagcgcca gacaggggcg gcatgggtgg 2341 agctggccaa agagttgcgg gaggtagccg ccgaggtgag cagcgccgcg cagagcgccc 2401 ggagcgcgtc gcgggggtgg cactggaagc tatggctaac cgtgatgctg gcttccatga 2461 tgcctacggt ggtgctgctg atcgcatcgt tgctcttgct cgacctgacg ccactgacaa 2521 ccgaggacgg ctcgatctgg ctgcgcttgg tggcccgatg aagaacgaca ggactttgca 2581 ggccataggc cgacagctca aggccatggg ctgtgagcgc ttcgatatcg gcgtcaggga 2641 cgccaccacc ggccagatga tgaaccggga atggtcagcc gccgaagtgc tccagaacac 2701 gccatggctc aagcggatga atgcccaggg caatgacgtg tatatcaggc ccgccgagca 2761 ggagcggcat ggtctggtgc tggtggacga cctcagcgag tttgacctgg atgacatgaa 2821 agccgagggc cgggagcctg ccctggtagt ggaaaccagc ccgaagaact atcaggcatg 2881 ggtcaaggtg gccgacgccg caggcggtga acttcggggg cagattgccc ggacgctggc 2941 cagcgagtac gacgccgacc cggccagcgc cgacagccgc cactatggcc gcttggcggg 3001 cttcaccaac cgcaaggaca agcacaccac ccgcgccggt tatcagccgt gggtgctgct 3061 gcgtgaatcc aagggcaaga ccgccaccgc tggcccggcg ctggtgcagc aggctggcca 3121 gcagatcgag caggcccagc ggcagcagga gaaggcccgc aggctggcca gcctcgaact 3181 gcccgagcgg cagcttagcc gccaccggcg cacggcgctg gacgagtacc gcagcgagat 3241 ggccgggctg gtcaagcgct tcggtgatga cctcagcaag tgcgacttta tcgccgcgca 3301 gaagctggcc agccggggcc gcagtgccga ggaaatcggc aaggccatgg ccgaggccag 3361 cccagcgctg gcagagcgca agcccggcca cgaagcggat tacatcgagc gcaccgtcag 3421 caaggtcatg ggtctgccca gcgtccagct tgcgcgggcc gagctggcac gggcaccggc 3481 accccgccag cgaggcatgg acaggggcgg gccagatttc agcatgtagt gcttgcgttg 3541 gtactcacgc ctgttatact atgagtactc acgcacagaa gggggtttta tggaatacga 3601 aaaaagcgct tcagggtcgg tctacctgat caaaagtgac aagggctatt ggttgcccgg 3661 tggctttggt tatacgtcaa acaaggccga ggctggccgc ttttcagtcg ctgatatggc 3721 cagccttaac cttgacggct gcaccttgtc cttgttccgc gaagacaagc ctttcggccc 3781 cggcaagttt ctcggtgact gatatgaaag accaaaagga caagcagacc ggcgacctgc 3841 tggccagccc tgacgctgta cgccaagcgc gatatgccga gcgcatgaag gccaaaggga 3901 tgcgtcagcg caagttctgg ctgaccgacg acgaatacga ggcgctgcgc gagtgcctgg 3961 aagaactcag agcggcgcag ggcgggggta gtgaccccgc cagcgcctaa ccaccaactg 4021 cctgcaaagg aggcaatcaa tggctaccca taagcctatc aatattctgg aggcgttcgc 4081 agcagcgccg ccaccgctgg actacgtttt gcccaacatg gtggccggta cggtcggggc 4141 gctggtgtcg cccggtggtg ccggtaaatc catgctggcc ctgcaactgg ccgcacagat 4201 tgcaggcggg ccggatctgc tggaggtggg cgaactgccc accggcccgg tgatctacct 4261 gcccgccgaa gacccgccca ccgccattca tcaccgcctg cacgcccttg gggcgcacct 4321 cagcgccgag gaacggcaag ccgtggctga cggcctgctg atccagccgc tgatcggcag 4381 cctgcccaac atcatggccc cggagtggtt cgacggcctc aagcgcgccg ccgagggccg 4441 ccgcctgatg gtgctggaca cgctgcgccg gttccacatc gaggaagaaa acgccagcgg 4501 ccccatggcc caggtcatcg gtcgcatgga ggccatcgcc gccgataccg ggtgctctat 4561 cgtgttcctg caccatgcca gcaagggcgc ggccatgatg ggcgcaggcg accagcagca 4621 ggccagccgg ggcagctcgg tactggtcga taacatccgc tggcagtcct acctgtcgag 4681 catgaccagc gccgaggccg aggaatgggg tgtggacgac gaccagcgcc ggttcttcgt 4741 ccgcttcggt gtgagcaagg ccaactatgg cgcaccgttc gctgatcggt ggttcaggcg 4801 gcatgacggc ggggtgctca agcccgccgt gctggagagg cagcgcaaga gcaagggggt 4861 gccccgtggt gaagcctaag aacaagcaca gcctcagcca cgtccggcac gacccggcgc 4921 actgtctggc ccccggcctg ttccgtgccc tcaagcgggg cgagcgcaag cgcagcaagc 4981 tggacgtgac gtatgactac ggcgacggca agcggatcga gttcagcggc ccggagccgc 5041 tgggcgctga tgatctgcgc atcctgcaag ggctggtggc catggctggg cctaatggcc 5101 tagtgcttgg cccggaaccc aagaccgaag gcggacggca gctccggctg ttcctggaac 5161 ccaagtggga ggccgtcacc gctgatgcca tggtggtcaa aggtagctat cgggcgctgg 5221 caaaggaaat cggggcagag gtcgatagtg gtggggcgct caagcacata caggactgca 5281 tcgagcgcct ttggaaggta tccatcatcg cccagaatgg ccgcaagcgg caggggtttc 5341 ggctgctgtc ggagtacgcc agcgacgagg cggacgggcg cctgtacgtg gccctgaacc 5401 ccttgatcgc gcaggccgtc atgggtggcg gccagcatgt gcgcatcagc atggacgagg 5461 tgcgggcgct ggacagcgaa accgcccgcc tgctgcacca gcggctgtgt ggctggatcg 5521 accccggcaa aaccggcaag gcttccatag ataccttgtg cggctatgtc tggccgtcag 5581 aggccagtgg ttcgaccatg cgcaagcgcc gccagcgggt gcgcgaggcg ttgccggagc 5641 tggtcgcgct gggctggacg gtaaccgagt tcgcggcggg caagtacgac atcacccggc 5701 ccaaggcggc aggctgaccc cccccactct attgtaaaca agacattttt atcttttata 5761 ttcaatggct tattttcctg ctaattggta ataccatgaa aaataccatg ctcagaaaag 5821 gcttaacaat attttgaaaa attgcctact gagcgctgcc gcacagctcc ataggccgct 5881 ttcctggctt tgcttccaga tgtatgctct tctgctcctg caggcatcgt ggtgtcacgc 5941 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga 6001 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt 6061 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc 6121 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa 6181 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa cacgggataa taccgcgcca 6241 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca 6301 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcact ttacacttta 6361 tgcttccggc tcgtatgtta ggaggtcttt atcatgtcaa tttatcaaga atttgttaat 6421 aaatatagtt taagtaaaac tctaagattt gagttaatcc cacagggtaa aacacttgaa 6481 aacataaaag caagaggttt gattttagat gatgagaaaa gagctaaaga ctacaaaaag 6541 gctaaacaaa taattgataa atatcatcag ttttttatag aggagatatt aagttcggtt 6601 tgtattagcg aagatttatt acaaaactat tctgatgttt attttaaact taaaaagagt 6661 gatgatgata atctacaaaa agattttaaa agtgcaaaag atacgataaa gaaacaaata 6721 tctgaatata taaaggactc agagaaattt aagaatttgt ttaatcaaaa ccttatcgat 6781 gctaaaaaag ggcaagagtc agatttaatt ctatggctaa agcaatctaa ggataatggt 6841 atagaactat ttaaagccaa tagtgatatc acagatatag atgaggcgtt agaaataatc 6901 aaatctttta aaggttggac aacttatttt aagggttttc atgaaaatag aaaaaatgtt 6961 tatagtagca atgatattcc tacatctatt atttatagga tagtagatga taatttgcct 7021 aaatttctag aaaataaagc taagtatgag agtttaaaag acaaagctcc agaagctata 7081 aactatgaac aaattaaaaa agatttggca gaagagctaa cctttgatat tgactacaaa 7141 acatctgaag ttaatcaaag agttttttca cttgatgaag tttttgagat agcaaacttt 7201 aataattatc taaatcaaag tggtattact aaatttaata ctattattgg tggtaaattt 7261 gtaaatggtg aaaatacaaa gagaaaaggt ataaatgaat atataaatct atactcacag 7321 caaataaatg ataaaacact caaaaaatat aaaatgagtg ttttatttaa gcaaatttta 7381 agtgatacag aatctaaatc ttttgtaatt gataagttag aagatgatag tgatgtagtt 7441 acaacgatgc aaagttttta tgagcaaata gcagctttta aaacagtaga agaaaaatct 7501 attaaagaaa cactatcttt attatttgat gatttaaaag ctcaaaaact tgatttgagt 7561 aaaatttatt ttaaaaatga taaatctctt actgatctat cacaacaagt ttttgatgat 7621 tatagtgtta ttggtacagc ggtactagaa tatataactc aacaaatagc acctaaaaat 7681 cttgataacc ctagtaagaa agagcaagaa ttaatagcca aaaaaactga aaaagcaaaa 7741 tacttatctc tagaaactat aaagcttgcc ttagaagaat ttaataagca tagagatata 7801 gataaacagt gtaggtttga agaaatactt gcaaactttg cggctattcc gatgatattt 7861 gatgaaatag ctcaaaacaa agacaatttg gcacagatat ctatcaaata tcaaaatcaa 7921 ggtaaaaaag acctacttca agctagtgcg gaagatgatg ttaaagctat caaggatctt 7981 ttagatcaaa ctaataatct cttacataaa ctaaaaatat ttcatattag tcagtcagaa 8041 gataaggcaa atattttaga caaggatgag catttttatc tagtatttga ggagtgctac 8101 tttgagctag cgaatatagt gcctctttat aacaaaatta gaaactatat aactcaaaag 8161 ccatatagtg atgagaaatt taagctcaat tttgagaact cgactttggc taatggttgg 8221 gataaaaata aagagcctga caatacggca attttattta tcaaagatga taaatattat 8281 ctgggtgtga tgaataagaa aaataacaaa atatttgatg ataaagctat caaagaaaat 8341 aaaggcgagg gttataaaaa aattgtttat aaacttttac ctggcgcaaa taaaatgtta 8401 cctaaggttt tcttttctgc taaatctata aaattttata atcctagtga agatatactt 8461 agaataagaa atcattccac acatacaaaa aatggtagtc ctcaaaaagg atatgaaaaa 8521 tttgagttta atattgaaga ttgccgaaaa tttatagatt tttataaaca gtctataagt 8581 aagcatccgg agtggaaaga ttttggattt agattttctg atactcaaag atataattct 8641 atagatgaat tttatagaga agttgaaaat caaggctaca aactaacttt tgaaaatata 8701 tcagagagct atattgatag cgtagttaat cagggtaaat tgtacctatt ccaaatctat 8761 aataaagatt tttcagctta tagcaaaggg cgaccaaatc tacatacttt atattggaaa 8821 gcgctgtttg atgagagaaa tcttcaagat gtggtttata agctaaatgg tgaggcagag 8881 cttttttatc gtaaacaatc aatacctaaa aaaatcactc acccagctaa agaggcaata 8941 gctaataaaa acaaagataa tcctaaaaaa gagagtgttt ttgaatatga tttaatcaaa 9001 gataaacgct ttactgaaga taagtttttc tttcactgtc ctattacaat caattttaaa 9061 tctagtggag ctaataagtt taatgatgaa atcaatttat tgctaaaaga aaaagcaaat 9121 gatgttcata tattaagtat agatagaggt gaaagacatt tagcttacta tactttggta 9181 gatggtaaag gcaatatcat caaacaagat actttcaaca tcattggtaa tgatagaatg 9241 aaaacaaact accatgataa gcttgctgca atagagaaag atagggattc agctaggaaa 9301 gactggaaaa agataaataa catcaaagag atgaaagagg gctatctatc tcaggtagtt 9361 catgaaatag ctaagctagt tatagagtat aatgctattg tggtttttga ggatttaaat 9421 tttggattta aaagagggcg tttcaaggta gagaagcagg tctatcaaaa gttagaaaaa 9481 atgctaattg agaaactaaa ctatctagtt ttcaaagata atgagtttga taaaactggg 9541 ggagtgctta gagcttatca gctaacagca ccttttgaga cttttaaaaa gatgggtaaa 9601 caaacaggta ttatctacta tgtaccagct ggttttactt caaaaatttg tcctgtaact 9661 ggttttgtaa atcagttata tcctaagtat gaaagtgtca gcaaatctca agagttcttt 9721 agtaagtttg acaagatttg ttataacctt gataagggct attttgagtt tagttttgat 9781 tataaaaact ttggtgacaa ggctgccaaa ggcaagtgga ctatagctag ctttgggagt 9841 agattgatta actttagaaa ttcagataaa aatcataatt gggatactcg agaagtttat 9901 ccaactaaag agttggagaa attgctaaaa gattattcta tcgaatatgg gcatggcgaa 9961 tgtatcaaag cagctatttg cggtgagagc gacaaaaagt tttttgctaa gctaactagt 10021 gtcctaaata ctatcttaca aatgcgtaac tcaaaaacag gtactgagtt agattatcta 10081 atttcaccag tagcagatgt aaatggcaat ttctttgatt cgcgacaggc gccaaaaaat 10141 atgcctcaag atgctgatgc caatggtgct tatcatattg ggctaaaagg tctgatgcta 10201 ctaggtagga tcaaaaataa tcaagagggc aaaaaactca atttggttat caaaaatgaa 10261 gagtattttg agttcgtgca gaataggaat aactaagaat tcttgacagc tagctcagtc 10321 ctaggtataa tgctagcgct gatttaggca aaaacgggtc taagaacttt aaataatttc 10381 tactgttgta gatgagagca ggtgtcagcc gctacagggc gcgtcccatt cgccattcag 10441 gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagctggc 10501 gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt cccagtcacg 10561 acgttgtaaa acgacggcca gtgagcgcgc gtaatacgac tcactatagg gcgaattggg 10621 taccgggccc cccctcgagg tcctccagct tttgttccct ttagtgaggg ttaattgcgc 10681 gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc 10741 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct 10801 aactcacatt aattgcgttg cgctcactgc ccgctttcca cccacctgca aaagtctaag 10861 aactttaaat aatttctact gttgtagatg ctactattcc tgtgccttca gataattcag 10921 tctaagaact ttaaataatt tctactgttg tagatgtcta gagccttttg tattagtagc 10981 cggtctaaga actttaaata atttctactg ttgtagatta gcgatttatg aaggtcattt 11041 ttttgtctag ctttaatgcg gtagttggta ccgtcgactc tagaggatct gtaatccggg 11101 cagcgcaacg gaacattcat cagtgtaaaa atggaatcaa taaagccctg cgcagcgcgc 11161 agggtcagcc tgaatacgcg tttaatgacc agcacagtcg tgatggcaag gtcagaatag 11221 cgctgaggtc tgcctcgtga agaaggtgtt gctgactcat accaggcctg aatcgcccca 11281 tcatccagcc agaaagtgag ggagccacgg ttgatgagag ctttgttgta ggtggaccag 11341 ttggtgattt tgaacttttg ctttgccacg gaacggtctg cgttgtcggg aagatgcgtg 11401 atctgatcct tcaactcagc aaaagttcga tttattcaac aaagccgccg tcccgtcaag 11461 tcagcgtaat gctctgccag tgttacaacc aattaaccaa ttctgattag aaaaactcat 11521 cgagcatcaa atgaaactgc aatttattca tatcaggatt atcaatacca tatttttgaa 11581 aaagccgttt ctgtaatgaa ggagaaaact caccgaggca gttccatagg atggcaagat 11641 cctggtatcg gtctgcgatt ccgactcgtc caacatcaat acaacctatt aatttcccct 11701 cgtcaaaaat aaggttatca agtgagaaat caccatgagt gacgactgaa tccggtgaga 11761 atggcaaaag cttatgcatt tctttccaga cttgttcaac aggccagcca ttacgctcgt 11821 catcaaaatc actcgcatca accaaaccgt tattcattcg tgattgcgcc tgagcgagac 11881 gaaatacgcg atcgctgtta aaaggacaat tacaaacagg aatcgaatgc aaccggcgca 11941 ggaacactgc cagcgcatca acaatatttt cacctgaatc aggatattct tctaatacct 12001 ggaatgctgt tttcccgggg atcgcagtgg tgagtaacca tgcatcatca ggagtacgga 12061 taaaatgctt gatggtcgga agaggcataa attccgtcag ccagtttagt ctgaccatct 12121 catctgtaac atcattggca acgctacctt tgccatgttt cagaaacaac tctggcgcat 12181 cgggcttccc atacaatcga tagattgtcg cacctgattg cccgacatta tcgcgagccc 12241 atttataccc atataaatca gcatccatgt tggaatttaa tcgcggcctc gagcaagacg 12301 tttcccgttg aatatggctc ataacacccc ttgtattact gtttatgtaa gcagacagtt 12361 ttattgttca tgatgatata tttttatctt gtgcaatgta acatcagaga ttttgagaca 12421 caacgtggct ttgttgaata aatcgaactt ttgctgagtt gaaggatcag atcacgcatc 12481 ttcccgacaa cgcagaccgt tccgtggcaa agcaaaagtt caaaatcacc aactggtcca 12541 cctacaacaa agctctcatc aaccgtggct ccctcacttt ctggctggat gatggggcga 12601 ttcaggcctg gtatgagtca gcaacacctt cttcacgagg cagacctcag cgctattctg 12661 accttgccat cacgactgtg ctgg //