LOCUS Exported 9338 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION BPNLS-Cas9-BPNLS-2AGFP expression plasmid. ACCESSION . VERSION . KEYWORDS pCAG-1BPNLS-Cas9-1BPNLS-2AGFP SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 9338) AUTHORS Suzuki K, Tsunekawa Y, Hernandez-Benitez R, Wu J, Zhu J, Kim EJ, Hatanaka F, Yamamoto M, Araoka T, Li Z, Kurita M, Hishida T, Li M, Aizawa E, Guo S, Chen S, Goebl A, Soligalla RD, Qu J, Jiang T, Fu X, Jafari M, Esteban CR, Berggren WT, Lajara J, Nunez-Delicado E, Guillen P, Campistol JM, Matsuzaki F, Liu GH, Magistretti P, Zhang K, Callaway EM, Zhang K, Belmonte JC TITLE In vivo genome editing via CRISPR/Cas9 mediated homology-independent targeted integration. JOURNAL Nature. 2016 Dec 1;540(7631):144-149. doi: 10.1038/nature20565. Epub 2016 Nov 16. PUBMED 27851729 REFERENCE 2 (bases 1 to 9338) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..9338 /organism="synthetic DNA construct" /mol_type="other DNA" enhancer 4..383 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 386..661 /label=chicken beta-actin promoter intron 662..1673 /label=chimeric intron /note="chimera between introns from chicken beta-actin and rabbit beta-globin" primer_bind 1597..1619 /label=pCAGGS-5 /note="Chimeric intron in CAG promoter, forward primer" primer_bind 1681..1700 /label=pCAG-F /note="Rabbit beta-globin intron, for pCAG plasmids, forward primer" primer_bind 1728..1747 /label=T7 /note="T7 promoter, forward primer" promoter 1728..1746 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" CDS 1794..1814 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" regulatory 1848..1857 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 1854..5957 /codon_start=1 /product="Cas9 (Csn1) endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system" /label=Cas9 /note="generates RNA-guided double strand breaks in DNA" /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL DATLIHQSITGLYETRIDLSQLGGD" CDS 6003..6023 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" CDS 6030..6095 /codon_start=1 /product="three tandem FLAG(R) epitope tags, followed by an enterokinase cleavage site" /label=3xFLAG /translation="DYKDHDGDYKDHDIDYKDDDDK" CDS 6117..6170 /codon_start=1 /product="2A peptide from Thosea asigna virus capsid protein" /label=T2A /note="Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides." /translation="EGRGSLLTCGDVEENPGP" CDS 6171..6884 /codon_start=1 /product="enhanced GFP" /label=EGFP /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE FVTAAGITLGMDELYK" primer_bind complement(6213..6234) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(6474..6493) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 6821..6842 /label=EGFP-C /note="EGFP, forward primer" primer_bind complement(6891..6910) /label=Bglob-pA-R /note="Rabbit beta-globin polyA region, reverse primer" polyA_signal 6956..7011 /label=-beta-globin poly(A) signal /note="rabbit beta-globin polyadenylation signal (Gil and Proudfoot, 1987)" primer_bind complement(7010..7029) /label=rbglobpA-R /note="Rabbit beta-globin polyA, reverse primer. Also called rb-glob-pA-term-R" primer_bind complement(7417..7434) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(7588..8176) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(7668..7687) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(8347..9207) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 8970..8989 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(9208..9312) /gene="bla" /label=AmpR promoter ORIGIN 1 gtcgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata 61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc 121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag 181 ggactttcca ttgacgtcaa tgggtggact atttacggta aactgcccac ttggcagtac 241 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg 301 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg 361 tattagtcat cgctattacc atgggtcgag gtgagcccca cgttctgctt cactctcccc 421 atctcccccc cctccccacc cccaattttg tatttattta ttttttaatt attttgtgca 481 gcgatggggg cggggggggg gggggcgcgc gccaggcggg gcggggcggg gcgaggggcg 541 gggcggggcg aggcggagag gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt 601 tccttttatg gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc 661 gggagtcgct gcgttgcctt cgccccgtgc cccgctccgc gccgcctcgc gccgcccgcc 721 ccggctctga ctgaccgcgt tactcccaca ggtgagcggg cgggacggcc cttctcctcc 781 gggctgtaat tagcgcttgg tttaatgacg gctcgtttct tttctgtggc tgcgtgaaag 841 ccttaaaggg ctccgggagg gccctttgtg cgggggggag cggctcgggg ggtgcgtgcg 901 tgtgtgtgtg cgtggggagc gccgcgtgcg gcccgcgctg cccggcggct gtgagcgctg 961 cgggcgcggc gcggggcttt gtgcgctccg cgtgtgcgcg aggggagcgc ggccgggggc 1021 ggtgccccgc ggtgcggggg ggctgcgagg ggaacaaagg ctgcgtgcgg ggtgtgtgcg 1081 tgggggggtg agcagggggt gtgggcgcgg cggtcgggct gtaacccccc cctgcacccc 1141 cctccccgag ttgctgagca cggcccggct tcgggtgcgg ggctccgtgc ggggcgtggc 1201 gcggggctcg ccgtgccggg cggggggtgg cggcaggtgg gggtgccggg cggggcgggg 1261 ccgcctcggg ccggggaggg ctcgggggag gggcgcggcg gccccggagc gccggcggct 1321 gtcgaggcgc ggcgagccgc agccattgcc ttttatggta atcgtgcgag agggcgcagg 1381 gacttccttt gtcccaaatc tgtgcggagc cgaaatctgg gaggcgccgc cgcaccccct 1441 ctagcgggcg cggggcgaag cggtgcggcg ccggcaggaa ggaaatgggc ggggagggcc 1501 ttcgtgcgtc gccgcgccgc cgtccccttc tccctctcca gcctcggggc tgtccgcggg 1561 gggacggctg ccttcggggg ggacggggca gggcggggtt cggcttctgg cgtgtgaccg 1621 gcggctctag agcctctgct aaccatgttc atgccttctt ctttttccta cagctcctgg 1681 gcaacgtgct ggttattgtg ctgtctcatc attttggcaa agaattctaa tacgactcac 1741 tatagggaga cgccaccatg aagcggactg ctgatggcag tgaatttgag tccccaaaga 1801 agaagagaaa ggtggaaggt atccacggag tcccagcagc cgaattcgcc accatggaca 1861 agaagtattc tatcggactg gacatcggga ctaatagcgt cgggtgggcc gtgatcactg 1921 acgagtacaa ggtgccctct aagaagttca aggtgctcgg gaacaccgac cggcattcca 1981 tcaagaaaaa tctgatcgga gctctcctct ttgattcagg ggagaccgct gaagcaaccc 2041 gcctcaagcg gactgctaga cggcggtaca ccaggaggaa gaaccggatt tgttaccttc 2101 aagagatatt ctccaacgaa atggcaaagg tcgacgacag cttcttccat aggctggaag 2161 aatcattcct cgtggaagag gataagaagc atgaacggca tcccatcttc ggtaatatcg 2221 tcgacgaggt ggcctatcac gagaaatacc caaccatcta ccatcttcgc aaaaagctgg 2281 tggactcaac cgacaaggca gacctccggc ttatctacct ggccctggcc cacatgatca 2341 agttcagagg ccacttcctg atcgagggcg acctcaatcc tgacaatagc gatgtggata 2401 aactgttcat ccagctggtg cagacttaca accagctctt tgaagagaac cccatcaatg 2461 caagcggagt cgatgccaag gccattctgt cagcccggct gtcaaagagc cgcagacttg 2521 agaatcttat cgctcagctg ccgggtgaaa agaaaaatgg actgttcggg aacctgattg 2581 ctctttcact tgggctgact cccaatttca agtctaattt cgacctggca gaggatgcca 2641 agctgcaact gtccaaggac acctatgatg acgatctcga caacctcctg gcccagatcg 2701 gtgaccaata cgccgacctt ttccttgctg ctaagaatct ttctgacgcc atcctgctgt 2761 ctgacattct ccgcgtgaac actgaaatca ccaaggcccc tctttcagct tcaatgatta 2821 agcggtatga tgagcaccac caggacctga ccctgcttaa ggcactcgtc cggcagcagc 2881 ttccggagaa gtacaaggaa atcttctttg accagtcaaa gaatggatac gccggctaca 2941 tcgacggagg tgcctcccaa gaggaatttt ataagtttat caaacctatc cttgagaaga 3001 tggacggcac cgaagagctc ctcgtgaaac tgaatcggga ggatctgctg cggaagcagc 3061 gcactttcga caatgggagc attccccacc agatccatct tggggagctt cacgccatcc 3121 ttcggcgcca agaggacttc tacccctttc ttaaggacaa cagggagaag attgagaaaa 3181 ttctcacttt ccgcatcccc tactacgtgg gacccctcgc cagaggaaat agccggtttg 3241 cttggatgac cagaaagtca gaagaaacta tcactccctg gaacttcgaa gaggtggtgg 3301 acaagggagc cagcgctcag tcattcatcg aacggatgac taacttcgat aagaacctcc 3361 ccaatgagaa ggtcctgccg aaacattccc tgctctacga gtactttacc gtgtacaacg 3421 agctgaccaa ggtgaaatat gtcaccgaag ggatgaggaa gcccgcattc ctgtcaggcg 3481 aacaaaagaa ggcaattgtg gaccttctgt tcaagaccaa tagaaaggtg accgtgaagc 3541 agctgaagga ggactatttc aagaaaattg aatgcttcga ctctgtggag attagcgggg 3601 tcgaagatcg gttcaacgca agcctgggta cctaccatga tctgcttaag atcatcaagg 3661 acaaggattt tctggacaat gaggagaacg aggacatcct tgaggacatt gtcctgactc 3721 tcactctgtt cgaggaccgg gaaatgatcg aggagaggct taagacctac gcccatctgt 3781 tcgacgataa agtgatgaag caacttaaac ggagaagata taccggatgg ggacgcctta 3841 gccgcaaact catcaacgga atccgggaca aacagagcgg aaagaccatt cttgatttcc 3901 ttaagagcga cggattcgct aatcgcaact tcatgcaact tatccatgat gattccctga 3961 cctttaagga ggacatccag aaggcccaag tgtctggaca aggtgactca ctgcacgagc 4021 atatcgcaaa tctggctggt tcacccgcta ttaagaaggg tattctccag accgtgaaag 4081 tcgtggacga gctggtcaag gtgatgggtc gccataaacc agagaacatt gtcatcgaga 4141 tggccaggga aaaccagact acccagaagg gacagaagaa cagcagggag cggatgaaaa 4201 gaattgagga agggattaag gagctcgggt cacagatcct taaagagcac ccggtggaaa 4261 acacccagct tcagaatgag aagctctatc tgtactacct tcaaaatgga cgcgatatgt 4321 atgtggacca agagcttgat atcaacaggc tctcagacta cgacgtggac cacatcgtcc 4381 ctcagagctt cctcaaagac gactcaattg acaataaggt gctgactcgc tcagacaaga 4441 accggggaaa gtcagataac gtgccctcag aggaagtcgt gaaaaagatg aagaactatt 4501 ggcgccagct tctgaacgca aagctgatca ctcagcggaa gttcgacaat ctcactaagg 4561 ctgagagggg cggactgagc gaactggaca aagcaggatt cattaaacgg caacttgtgg 4621 agactcggca gattactaaa catgtcgccc aaatccttga ctcacgcatg aataccaagt 4681 acgacgaaaa cgacaaactt atccgcgagg tgaaggtgat taccctgaag tccaagctgg 4741 tcagcgattt cagaaaggac tttcaattct acaaagtgcg ggagatcaat aactatcatc 4801 atgctcatga cgcatatctg aatgccgtgg tgggaaccgc cctgatcaag aagtacccaa 4861 agctggaaag cgagttcgtg tacggagact acaaggtcta cgacgtgcgc aagatgattg 4921 ccaaatctga gcaggagatc ggaaaggcca ccgcaaagta cttcttctac agcaacatca 4981 tgaatttctt caagaccgaa atcacccttg caaacggtga gatccggaag aggccgctca 5041 tcgagactaa tggggagact ggcgaaatcg tgtgggacaa gggcagagat ttcgctaccg 5101 tgcgcaaagt gctttctatg cctcaagtga acatcgtgaa gaaaaccgag gtgcaaaccg 5161 gaggcttttc taaggaatca atcctcccca agcgcaactc cgacaagctc attgcaagga 5221 agaaggattg ggaccctaag aagtacggcg gattcgattc accaactgtg gcttattctg 5281 tcctggtcgt ggctaaggtg gaaaaaggaa agtctaagaa gctcaagagc gtgaaggaac 5341 tgctgggtat caccattatg gagcgcagct ccttcgagaa gaacccaatt gactttctcg 5401 aagccaaagg ttacaaggaa gtcaagaagg accttatcat caagctccca aagtatagcc 5461 tgttcgaact ggagaatggg cggaagcgga tgctcgcctc cgctggcgaa cttcagaagg 5521 gtaatgagct ggctctcccc tccaagtacg tgaatttcct ctaccttgca agccattacg 5581 agaagctgaa ggggagcccc gaggacaacg agcaaaagca actgtttgtg gagcagcata 5641 agcattatct ggacgagatc attgagcaga tttccgagtt ttctaaacgc gtcattctcg 5701 ctgatgccaa cctcgataaa gtccttagcg catacaataa gcacagagac aaaccaattc 5761 gggagcaggc tgagaatatc atccacctgt tcaccctcac caatcttggt gcccctgccg 5821 cattcaagta cttcgacacc accatcgacc ggaaacgcta tacctccacc aaagaagtgc 5881 tggacgccac cctcatccac cagagcatca ccggacttta cgaaactcgg attgacctct 5941 cacagctcgg aggggatgag ggagctgata agcgcaccgc cgatggttcc gagttcgaaa 6001 gccccaaaaa aaagcgcaag gtcgaagatg actataagga tcacgacggt gattataaag 6061 accacgatat cgactataaa gacgacgatg acaaagagct gtataaactg ctgttcgaag 6121 gaaggggctc tttgcttact tgtggagatg ttgaggaaaa tccaggaccc gtgagcaagg 6181 gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg 6241 gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc 6301 tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc 6361 tgacctacgg cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct 6421 tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg 6481 gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg 6541 agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca 6601 actacaacag ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga 6661 acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc 6721 agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc 6781 agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg 6841 tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagtgaaga tctttttccc 6901 tctgccaaaa attatgggga catcatgaag ccccttgagc atctgacttc tggctaataa 6961 aggaaattta ttttcattgc aatagtgtgt tggaattttt tgtgtctctc actcggaagg 7021 acatatggga gggcaaatca tttaaaacat cagaatgagt atttggttta gagtttggca 7081 acatatgccc atatgctggc tgccatgaac aaaggttggc tataaagagg tcatcagtat 7141 atgaaacagc cccctgctgt ccattcctta ttccatagaa aagccttgac ttgaggttag 7201 atttttttta tattttgttt tgtgttattt ttttctttaa catccctaaa attttcctta 7261 catgttttac tagccagatt tttcctcctc tcctgactac tcccagtcat agctgtccct 7321 cttctcttat gaagatccct cgaccgatcc gctgcattaa tgaatcggcc aacgcgcggg 7381 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc 7441 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac 7501 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa 7561 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca 7621 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc 7681 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata 7741 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct caatgctcac gctgtaggta 7801 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca 7861 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga 7921 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg 7981 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg 8041 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg 8101 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag 8161 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa 8221 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat 8281 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc 8341 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc 8401 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc 8461 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc 8521 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc 8581 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt 8641 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc 8701 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa 8761 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt 8821 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg 8881 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc 8941 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa 9001 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt 9061 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt 9121 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag 9181 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta 9241 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat 9301 aggggttccg cgcacatttc cccgaaaagt gccacctg //