LOCUS Exported 2007 bp ds-DNA linear SYN 09-JUN-2021 DEFINITION Bioluminescent and ratiometric voltage indicator. ACCESSION . VERSION . KEYWORDS LOTUS-V/pcDNA3 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2007) AUTHORS Inagaki S, Tsutsui H, Suzuki K, Agetsuma M, Arai Y, Jinno Y, Bai G, Daniels MJ, Okamura Y, Matsuda T, Nagai T TITLE Genetically encoded bioluminescent voltage indicator for multi-purpose use in wide range of bioimaging. JOURNAL Sci Rep. 2017 Feb 13;7:42398. doi: 10.1038/srep42398. PUBMED 28205521 REFERENCE 2 (bases 1 to 2007) AUTHORS . TITLE Direct Submission JOURNAL Exported Jun 9, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..2007 /organism="synthetic DNA construct" /mol_type="other DNA" CDS 316..828 /codon_start=1 /product="NanoLuc(R) luciferase" /label=Nluc /note="human codon-optimized" /translation="MVFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQR IVLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGV TPNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGW RLCERILA" CDS 1288..2007 /codon_start=1 /product="yellow fluorescent protein (YFP) with fast and efficient maturation (Nagai et al., 2002)" /label=Venus /note="mammalian codon-optimized" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL EFVTAAGITLGMDELYK" primer_bind complement(1333..1354) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(1594..1613) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 1941..1962 /label=EGFP-C /note="EGFP, forward primer" ORIGIN 1 atggagggat tcgacggttc agattttagt cctccagctg atttagttgg cgttgacggt 61 gcagtcatgc ggaacgtcgt tgacgtcacg ataaatggtg acgtcactgc tccgccgaaa 121 gctgcgccaa gaaaaagtga atcggtaaag aaagttcatt ggaatgatgt agaccaagga 181 ccgagtgaaa aaccagagac aagacaggag gaacgaatag atatacccga gatttcaggt 241 ctatggtggg gcgagaatga acatggagtg gacgatggga gaatggagat acctactact 301 ggtgtagggg atcccatggt cttcacactc gaagatttcg ttggggactg gcgacagaca 361 gccggctaca acctggacca agtccttgaa cagggaggtg tgtccagttt gtttcagaat 421 ctcggggtgt ccgtaactcc gatccaaagg attgtcctga gcggtgaaaa tgggctgaag 481 atcgacatcc atgtcatcat cccgtatgaa ggtctgagcg gcgaccaaat gggccagatc 541 gaaaaaattt ttaaggtggt gtaccctgtg gatgatcatc actttaaggt gatcctgcac 601 tatggcacac tggtaatcga cggggttacg ccgaacatga tcgactattt cggacggccg 661 tatgaaggca tcgccgtgtt cgacggcaaa aagatcactg taacagggac cctgtggaac 721 ggcaacaaaa ttatcgacga gcgcctgatc aaccccgacg gctccctgct gttccgagta 781 accatcaacg gagtgaccgg ctggcggctg tgcgaacgca ttctggcgga attcggcgtg 841 ggccgcgtcc agtttcgtgt ccgagcagtg attgatcatc tagggatgcg agtctttgga 901 gtcttcctaa ttttcttgga catcatcctc atgatcattg atctcagtct tccaggaaaa 961 agtgaatctt cacaatcctt ttatgacggg atggctttgg ctctttcttg ttatttcatg 1021 ctggatttag gattaaggat atttgcctac gggcccaaga atttcttcac caacccctgg 1081 gaggttgctg atggtttgat tatcgttgtc acattcgtcg tcacgatatt ttacactgtg 1141 ttagatgaat acgtgcaaga aacaggagcc gatggtttgg ggcagttggt tgtgttggcc 1201 cgtttgctgc gtgtggttcg attagcaaga atattttatt cccatcaaca aatgaaggct 1261 tcaagcagaa gaacaatatc actcgagatg gtgagcaagg gcgaggagct gttcaccggg 1321 gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc 1381 ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagctgat ctgcaccacc 1441 ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgggctacgg cctgcagtgc 1501 ttcgcccgct accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa 1561 ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc 1621 gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc 1681 aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag ccacaacgtc 1741 tatatcaccg ccgacaagca gaagaacggc atcaaggcca acttcaagat ccgccacaac 1801 atcgaggacg gcggcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac 1861 ggccccgtgc tgctgcccga caaccactac ctgagctacc agtccgccct gagcaaagac 1921 cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact 1981 ctcggcatgg acgagctgta caagtaa //