LOCUS Exported 2063 bp ds-DNA linear SYN 08-JUN-2021 DEFINITION Destination vector for domain insertion. ACCESSION . VERSION . KEYWORDS pTALE-STAR-A_RFP entry SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 2063) AUTHORS Gogolok S, Garcia-Diaz C, Pollard SM TITLE STAR: a simple TAL effector assembly reaction using isothermal assembly. JOURNAL Sci Rep. 2016 Sep 12;6:33209. doi: 10.1038/srep33209. PUBMED 27615025 REFERENCE 2 (bases 1 to 2063) AUTHORS . TITLE Direct Submission JOURNAL Exported Jun 8, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..2063 /organism="synthetic DNA construct" /mol_type="other DNA" CDS 4..69 /codon_start=1 /product="three tandem FLAG(R) epitope tags, followed by an enterokinase cleavage site" /label=3xFLAG /translation="DYKDHDGDYKDHDIDYKDDDDK" CDS 76..96 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" CDS 879..899 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" misc_feature 935..956 /label=BioBrick prefix /note="BioBrick prefix for parts that do not start with ""ATG""" protein_bind 1057..1078 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 1093..1123 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 1131..1147 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." RBS 1165..1176 /note="strong bacterial ribosome binding site (Elowitz and Leibler, 2000)" CDS 1183..1860 /codon_start=1 /product="monomeric derivative of DsRed (Campbell et al., 2002)" /label=mRFP1 /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS TGA" terminator 1905..1976 /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" terminator 1992..2019 /label=T7Te terminator /note="phage T7 early transcription terminator" misc_feature 2026..2046 /label=BioBrick suffix /note="universal suffix for all parts" ORIGIN 1 atggactaca aagaccatga cggtgattat aaagatcatg acatcgatta caaggatgac 61 gatgacaaga tggcccccaa gaagaagagg aaggtgggca ttcaccgcgg ggtacctatg 121 gtggacttga ggacactcgg ttattcgcaa cagcaacagg agaaaatcaa gcctaaggtc 181 aggagcaccg tcgcgcaaca ccacgaggcg cttgtggggc atggcttcac tcatgcgcat 241 attgtcgcgc tttcacagca ccctgcggcg cttgggacgg tggctgtcaa ataccaagat 301 atgattgcgg ccctgcccga agccacgcac gaggcaattg taggggtcgg taaacagtgg 361 tcgggagcgc gagcacttga ggcgctgctg actgtggcgg gtgagcttag ggggcctccg 421 ctccagctcg acaccgggca gctgctgaag atcgcgaaga gagggggagt aacagcggta 481 gaggcagtgc acgcctggcg caatgcgctc accggggccc ccttgaacct gcaggatacg 541 tttatcagtg ataaagtgtc aagcatgaca aagttgcagc cgaatacagt ggtatcctgc 601 aggtgctctg tcaggaccac gggcttacgc cacaacaggt ggtggcgatt gcatctaaca 661 ttggcggacg ccctgccttg gtgtccatcg tagcccaatt gtccaggccc gatcccgcgt 721 tggctgcgtt aacgaatgac catctggtgg cgttggcatg tcttggtgga cgacccgcgc 781 tcgatgcagt caaaaagggt ctgcctcatg ctcccgcatt gatcaaaaga accaaccggc 841 ggattcccga gagaacttcc catcgagtcg cggctagccc caagaagaag agaaaggtgg 901 aggccagcgg ttccggacag tagagacgcc attggaattc gcggccgctt ctagagcaat 961 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt 1021 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta 1081 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg 1141 ataacaattt cacacatact agagaaagag gagaaatact agatggcttc ctccgaagac 1201 gttatcaaag agttcatgcg tttcaaagtt cgtatggaag gttccgttaa cggtcacgag 1261 ttcgaaatcg aaggtgaagg tgaaggtcgt ccgtacgaag gtacccagac cgctaaactg 1321 aaagttacca aaggtggtcc gctgccgttc gcttgggaca tcctgtcccc gcagttccag 1381 tacggttcca aagcttacgt taaacacccg gctgacatcc cggactacct gaaactgtcc 1441 ttcccggaag gtttcaaatg ggaacgtgtt atgaacttcg aagacggtgg tgttgttacc 1501 gttacccagg actcctccct gcaagacggt gagttcatct acaaagttaa actgcgtggt 1561 accaacttcc cgtccgacgg tccggttatg cagaaaaaaa ccatgggttg ggaagcttcc 1621 accgaacgta tgtacccgga agacggtgct ctgaaaggtg aaatcaaaat gcgtctgaaa 1681 ctgaaagacg gtggtcacta cgacgctgaa gttaaaacca cctacatggc taaaaaaccg 1741 gttcagctgc cgggtgctta caaaaccgac atcaaactgg acatcacctc ccacaacgaa 1801 gactacacca tcgttgaaca gtacgaacgt gctgaaggtc gtcactccac cggtgcttaa 1861 taacgctgat agtgctagtg tagatcgcta ctagagccag gcatcaaata aaacgaaagg 1921 ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact 1981 agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttatatacta gtagcggccg 2041 ctgcagtcag accgtctcaa gct //