LOCUS Exported 9291 bp ds-DNA circular SYN 13-MAY-2021 DEFINITION Encodes gRNA for 3' target of human ZSCAN9. ACCESSION . VERSION . KEYWORDS PX458_ZSCAN9_1 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 9291) AUTHORS Savic D, Partridge EC, Newberry KM, Smith SB, Meadows SK, Roberts BS, Mackiewicz M, Mendenhall EM, Myers RM TITLE CETCh-seq: CRISPR epitope tagging ChIP-seq of DNA-binding proteins. JOURNAL Genome Res. 2015 Sep 9. PUBMED 26355004 REFERENCE 2 (bases 1 to 9291) AUTHORS . TITLE Direct Submission JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..9291 /organism="synthetic DNA construct" /mol_type="other DNA" rep_origin 42..630 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 531..550 /label=pBR322ori-F /note="pBR322 origin, forward primer" promoter 692..932 /label=U6 promoter /note="RNA polymerase III promoter for human U6 snRNA" primer_bind 692..712 /label=hU6-F /note="Human U6 promoter, forward primer" primer_bind 863..882 /label=LKO.1 5' /note="Human U6 promoter, forward primer" misc_RNA 962..1037 /label=gRNA scaffold /note="guide RNA scaffold for the Streptococcus pyogenes CRISPR/Cas9 system" enhancer 1134..1419 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer; contains an 18-bp deletion relative to the standard CMV enhancer" promoter 1421..1698 /label=chicken beta-actin promoter intron 1699..1926 /label=hybrid intron /note="hybrid between chicken beta-actin (CBA) and minute virus of mice (MMV) introns (Gray et al., 2011)" regulatory 1938..1947 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 1947..2012 /codon_start=1 /product="three tandem FLAG(R) epitope tags, followed by an enterokinase cleavage site" /label=3xFLAG /translation="DYKDHDGDYKDHDIDYKDDDDK" CDS 2019..2039 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" CDS 2064..6164 /codon_start=1 /product="Cas9 (Csn1) endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system" /label=Cas9 /note="generates RNA-guided double strand breaks in DNA" /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD ATLIHQSITGLYETRIDLSQLGGD" CDS 6165..6212 /codon_start=1 /product="bipartite nuclear localization signal from nucleoplasmin" /label=nucleoplasmin NLS /translation="KRPAATKKAGQAKKKK" CDS 6228..6281 /codon_start=1 /product="2A peptide from Thosea asigna virus capsid protein" /label=T2A /note="Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides." /translation="EGRGSLLTCGDVEENPGP" CDS 6282..6995 /codon_start=1 /product="enhanced GFP" /label=EGFP /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE FVTAAGITLGMDELYK" CDS 6282..6995 /codon_start=1 /product="the original enhanced GFP (Yang et al., 1996)" /label=EGFP /note="mammalian codon-optimized" /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE FVTAAGITLGMDELYK" primer_bind complement(6324..6345) /label=EGFP-N /note="EGFP, reverse primer" primer_bind complement(6585..6604) /label=EXFP-R /note="For distinguishing EGFP variants, reverse primer" primer_bind 6932..6953 /label=EGFP-C /note="EGFP, forward primer" primer_bind complement(7023..7040) /label=BGH-rev /note="Bovine growth hormone terminator, reverse primer. Also called BGH reverse" polyA_signal 7029..7236 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" repeat_region 7245..7385 /label=AAV2 ITR /note="inverted terminal repeat of adeno-associated virus serotype 2" repeat_region 7245..7374 /label=AAV2 ITR rep_origin 7460..7915 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(7547..7566) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 7757..7778 /label=F1ori-F /note="F1 origin, forward primer" primer_bind complement(7932..7951) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind 8051..8073 /label=pGEX 3' /note="pGEX vectors, reverse primer" primer_bind complement(8111..8129) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 8197..8301 /gene="bla" /label=AmpR promoter CDS 8302..9162 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(8520..8539) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" ORIGIN 1 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg 61 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg 121 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca 181 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg 241 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg 301 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga 361 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac 421 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat 481 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc 541 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga 601 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc 661 ctggcctttt gctggccttt tgctcacatg tgagggccta tttcccatga ttccttcata 721 tttgcatata cgatacaagg ctgttagaga gataattgga attaatttga ctgtaaacac 781 aaagatatta gtacaaaata cgtgacgtag aaagtaataa tttcttgggt agtttgcagt 841 tttaaaatta tgttttaaaa tggactatca tatgcttacc gtaacttgaa agtatttcga 901 tttcttggct ttatatatct tgtggaaagg acgaaacacc gacagtggct gagctggtct 961 agttttagag ctagaaatag caagttaaaa taaggctagt ccgttatcaa cttgaaaaag 1021 tggcaccgag tcggtgcttt tttgttttag agctagaaat agcaagttaa aataaggcta 1081 gtccgttttt agcgcgtgcg ccaattctgc agacaaatgg ctctagaggt acccgttaca 1141 taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca 1201 atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact 1261 gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat 1321 gacggtaaat ggcccgcctg gcattgtgcc cagtacatga ccttatggga ctttcctact 1381 tggcagtaca tctacgtatt agtcatcgct attaccatgg tcgaggtgag ccccacgttc 1441 tgcttcactc tccccatctc ccccccctcc ccacccccaa ttttgtattt atttattttt 1501 taattatttt gtgcagcgat gggggcgggg gggggggggg ggcgcgcgcc aggcggggcg 1561 gggcggggcg aggggcgggg cggggcgagg cggagaggtg cggcggcagc caatcagagc 1621 ggcgcgctcc gaaagtttcc ttttatggcg aggcggcggc ggcggcggcc ctataaaaag 1681 cgaagcgcgc ggcgggcggg agtcgctgcg cgctgccttc gccccgtgcc ccgctccgcc 1741 gccgcctcgc gccgcccgcc ccggctctga ctgaccgcgt tactcccaca ggtgagcggg 1801 cgggacggcc cttctcctcc gggctgtaat tagctgagca agaggtaagg gtttaaggga 1861 tggttggttg gtggggtatt aatgtttaat tacctggagc acctgcctga aatcactttt 1921 tttcaggttg gaccggtgcc accatggact ataaggacca cgacggagac tacaaggatc 1981 atgatattga ttacaaagac gatgacgata agatggcccc aaagaagaag cggaaggtcg 2041 gtatccacgg agtcccagca gccgacaaga agtacagcat cggcctggac atcggcacca 2101 actctgtggg ctgggccgtg atcaccgacg agtacaaggt gcccagcaag aaattcaagg 2161 tgctgggcaa caccgaccgg cacagcatca agaagaacct gatcggagcc ctgctgttcg 2221 acagcggcga aacagccgag gccacccggc tgaagagaac cgccagaaga agatacacca 2281 gacggaagaa ccggatctgc tatctgcaag agatcttcag caacgagatg gccaaggtgg 2341 acgacagctt cttccacaga ctggaagagt ccttcctggt ggaagaggat aagaagcacg 2401 agcggcaccc catcttcggc aacatcgtgg acgaggtggc ctaccacgag aagtacccca 2461 ccatctacca cctgagaaag aaactggtgg acagcaccga caaggccgac ctgcggctga 2521 tctatctggc cctggcccac atgatcaagt tccggggcca cttcctgatc gagggcgacc 2581 tgaaccccga caacagcgac gtggacaagc tgttcatcca gctggtgcag acctacaacc 2641 agctgttcga ggaaaacccc atcaacgcca gcggcgtgga cgccaaggcc atcctgtctg 2701 ccagactgag caagagcaga cggctggaaa atctgatcgc ccagctgccc ggcgagaaga 2761 agaatggcct gttcggaaac ctgattgccc tgagcctggg cctgaccccc aacttcaaga 2821 gcaacttcga cctggccgag gatgccaaac tgcagctgag caaggacacc tacgacgacg 2881 acctggacaa cctgctggcc cagatcggcg accagtacgc cgacctgttt ctggccgcca 2941 agaacctgtc cgacgccatc ctgctgagcg acatcctgag agtgaacacc gagatcacca 3001 aggcccccct gagcgcctct atgatcaaga gatacgacga gcaccaccag gacctgaccc 3061 tgctgaaagc tctcgtgcgg cagcagctgc ctgagaagta caaagagatt ttcttcgacc 3121 agagcaagaa cggctacgcc ggctacattg acggcggagc cagccaggaa gagttctaca 3181 agttcatcaa gcccatcctg gaaaagatgg acggcaccga ggaactgctc gtgaagctga 3241 acagagagga cctgctgcgg aagcagcgga ccttcgacaa cggcagcatc ccccaccaga 3301 tccacctggg agagctgcac gccattctgc ggcggcagga agatttttac ccattcctga 3361 aggacaaccg ggaaaagatc gagaagatcc tgaccttccg catcccctac tacgtgggcc 3421 ctctggccag gggaaacagc agattcgcct ggatgaccag aaagagcgag gaaaccatca 3481 ccccctggaa cttcgaggaa gtggtggaca agggcgcttc cgcccagagc ttcatcgagc 3541 ggatgaccaa cttcgataag aacctgccca acgagaaggt gctgcccaag cacagcctgc 3601 tgtacgagta cttcaccgtg tataacgagc tgaccaaagt gaaatacgtg accgagggaa 3661 tgagaaagcc cgccttcctg agcggcgagc agaaaaaggc catcgtggac ctgctgttca 3721 agaccaaccg gaaagtgacc gtgaagcagc tgaaagagga ctacttcaag aaaatcgagt 3781 gcttcgactc cgtggaaatc tccggcgtgg aagatcggtt caacgcctcc ctgggcacat 3841 accacgatct gctgaaaatt atcaaggaca aggacttcct ggacaatgag gaaaacgagg 3901 acattctgga agatatcgtg ctgaccctga cactgtttga ggacagagag atgatcgagg 3961 aacggctgaa aacctatgcc cacctgttcg acgacaaagt gatgaagcag ctgaagcggc 4021 ggagatacac cggctggggc aggctgagcc ggaagctgat caacggcatc cgggacaagc 4081 agtccggcaa gacaatcctg gatttcctga agtccgacgg cttcgccaac agaaacttca 4141 tgcagctgat ccacgacgac agcctgacct ttaaagagga catccagaaa gcccaggtgt 4201 ccggccaggg cgatagcctg cacgagcaca ttgccaatct ggccggcagc cccgccatta 4261 agaagggcat cctgcagaca gtgaaggtgg tggacgagct cgtgaaagtg atgggccggc 4321 acaagcccga gaacatcgtg atcgaaatgg ccagagagaa ccagaccacc cagaagggac 4381 agaagaacag ccgcgagaga atgaagcgga tcgaagaggg catcaaagag ctgggcagcc 4441 agatcctgaa agaacacccc gtggaaaaca cccagctgca gaacgagaag ctgtacctgt 4501 actacctgca gaatgggcgg gatatgtacg tggaccagga actggacatc aaccggctgt 4561 ccgactacga tgtggaccat atcgtgcctc agagctttct gaaggacgac tccatcgaca 4621 acaaggtgct gaccagaagc gacaagaacc ggggcaagag cgacaacgtg ccctccgaag 4681 aggtcgtgaa gaagatgaag aactactggc ggcagctgct gaacgccaag ctgattaccc 4741 agagaaagtt cgacaatctg accaaggccg agagaggcgg cctgagcgaa ctggataagg 4801 ccggcttcat caagagacag ctggtggaaa cccggcagat cacaaagcac gtggcacaga 4861 tcctggactc ccggatgaac actaagtacg acgagaatga caagctgatc cgggaagtga 4921 aagtgatcac cctgaagtcc aagctggtgt ccgatttccg gaaggatttc cagttttaca 4981 aagtgcgcga gatcaacaac taccaccacg cccacgacgc ctacctgaac gccgtcgtgg 5041 gaaccgccct gatcaaaaag taccctaagc tggaaagcga gttcgtgtac ggcgactaca 5101 aggtgtacga cgtgcggaag atgatcgcca agagcgagca ggaaatcggc aaggctaccg 5161 ccaagtactt cttctacagc aacatcatga actttttcaa gaccgagatt accctggcca 5221 acggcgagat ccggaagcgg cctctgatcg agacaaacgg cgaaaccggg gagatcgtgt 5281 gggataaggg ccgggatttt gccaccgtgc ggaaagtgct gagcatgccc caagtgaata 5341 tcgtgaaaaa gaccgaggtg cagacaggcg gcttcagcaa agagtctatc ctgcccaaga 5401 ggaacagcga taagctgatc gccagaaaga aggactggga ccctaagaag tacggcggct 5461 tcgacagccc caccgtggcc tattctgtgc tggtggtggc caaagtggaa aagggcaagt 5521 ccaagaaact gaagagtgtg aaagagctgc tggggatcac catcatggaa agaagcagct 5581 tcgagaagaa tcccatcgac tttctggaag ccaagggcta caaagaagtg aaaaaggacc 5641 tgatcatcaa gctgcctaag tactccctgt tcgagctgga aaacggccgg aagagaatgc 5701 tggcctctgc cggcgaactg cagaagggaa acgaactggc cctgccctcc aaatatgtga 5761 acttcctgta cctggccagc cactatgaga agctgaaggg ctcccccgag gataatgagc 5821 agaaacagct gtttgtggaa cagcacaagc actacctgga cgagatcatc gagcagatca 5881 gcgagttctc caagagagtg atcctggccg acgctaatct ggacaaagtg ctgtccgcct 5941 acaacaagca ccgggataag cccatcagag agcaggccga gaatatcatc cacctgttta 6001 ccctgaccaa tctgggagcc cctgccgcct tcaagtactt tgacaccacc atcgaccgga 6061 agaggtacac cagcaccaaa gaggtgctgg acgccaccct gatccaccag agcatcaccg 6121 gcctgtacga gacacggatc gacctgtctc agctgggagg cgacaaaagg ccggcggcca 6181 cgaaaaaggc cggccaggca aaaaagaaaa aggaattcgg cagtggagag ggcagaggaa 6241 gtctgctaac atgcggtgac gtcgaggaga atcctggccc agtgagcaag ggcgaggagc 6301 tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt 6361 tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca 6421 tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgacctacg 6481 gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg 6541 ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca 6601 agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg 6661 gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacaaca 6721 gccacaacgt ctatatcatg gccgacaagc agaagaacgg catcaaggtg aacttcaaga 6781 tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc 6841 ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc cagtccgccc 6901 tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg 6961 ccgggatcac tctcggcatg gacgagctgt acaaggaatt ctaactagag ctcgctgatc 7021 agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc 7081 cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc 7141 gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg 7201 ggaggattgg gaagagaata gcaggcatgc tggggagcgg ccgcaggaac ccctagtgat 7261 ggagttggcc actccctctc tgcgcgctcg ctcgctcact gaggccgggc gaccaaaggt 7321 cgcccgacgc ccgggctttg cccgggcggc ctcagtgagc gagcgagcgc gcagctgcct 7381 gcaggggcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat 7441 acgtcaaagc aaccatagta cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg 7501 gttacgcgca gcgtgaccgc tacacttgcc agcgccttag cgcccgctcc tttcgctttc 7561 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc 7621 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgatttgggt 7681 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag 7741 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ctctatctcg 7801 ggctattctt ttgatttata agggattttg ccgatttcgg tctattggtt aaaaaatgag 7861 ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgtttac aattttatgg 7921 tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagccccg acacccgcca 7981 acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct 8041 gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg 8101 agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt 8161 tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt 8221 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa 8281 taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt 8341 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat 8401 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag 8461 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg 8521 ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata 8581 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat 8641 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc 8701 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg 8761 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac 8821 gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact 8881 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa 8941 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct 9001 ggagccggtg agcgtggaag ccgcggtatc attgcagcac tggggccaga tggtaagccc 9061 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga 9121 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac 9181 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag 9241 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt c //