LOCUS       Exported                6208 bp ds-DNA     circular SYN 23-AUG-2023
DEFINITION  AAV-mediated expression of tdTomato (codon diversified) under the 
            CAG promoter.
ACCESSION   .
VERSION     .
KEYWORDS    pAAV-CAG-tdTomato (codon diversified)
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6208)
  TITLE     Accessory plasmids for optogenetics
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6208)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 23, 2023 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6208
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     repeat_region   2..131
                     /label=AAV2 ITR
     promoter        442..719
                     /label=chicken beta-actin promoter
     primer_bind     998..1020
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1082..1101
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      1140..1149
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     misc_feature    2601..3189
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2654..2674)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3072..3083)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    3232..3353
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3243..3262)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3297..3316
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     repeat_region   3515..3644
                     /label=AAV2 ITR
     primer_bind     complement(3762..3779)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3933..4521)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4013..4032)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(4692..5552)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5315..5334
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5553..5657)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(5683..6138)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5820..5841)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6032..6051
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc gggcgacctt
       61 tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca actccatcac
      121 taggggttcc ttgtagttaa tgattaaccc gccatgctac ttatctacgt agccatgctc
      181 taggaagagt accattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      241 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      301 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      361 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      421 tagtcatcgc tattaccatg gtcgaggtga gccccacgtt ctgcttcact ctccccatct
      481 cccccccctc cccaccccca attttgtatt tatttatttt ttaattattt tgtgcagcga
      541 tgggggcggg gggggggggg gggcgcgcgc caggcggggc ggggcggggc gaggggcggg
      601 gcggggcgag gcggagaggt gcggcggcag ccaatcagag cggcgcgctc cgaaagtttc
      661 cttttatggc gaggcggcgg cggcggcggc cctataaaaa gcgaagcgcg cggcgggcgg
      721 gagtcgctgc gcgctgcctt cgccccgtgc cccgctccgc cgccgcctcg cgccgcccgc
      781 cccggctctg actgaccgcg ttactcccac aggtgagcgg gcgggacggc ccttctcctc
      841 cgggctgtaa ttagcgcttg gtttaatgac ggcttgtttc ttttctgtgg ctgcgtgaaa
      901 gccttgaggg gctccgggag ggccctttgt gcggggggag cggctcgggg ctgtccgcgg
      961 ggggacggct gccttcgggg gggacggggc agggcggggt tcggcttctg gcgtgtgacc
     1021 ggcggctcta gagcctctgc taaccatgtt catgccttct tctttttcct acagctcctg
     1081 ggcaacgtgc tggttattgt gctgtctcat cattttggca aagaattgga tccggtaccg
     1141 ccaccatggt gagtaagggc gaggaagtga tcaaagagtt catgcggttt aaggtgagaa
     1201 tggaaggaag catgaacggc cacgagttcg aaattgaggg agaaggagag ggacggccct
     1261 acgagggcac ccagacagcc aagctgaaag tgacaaaggg cgggcctctg ccattcgctt
     1321 gggacatcct gagcccacag tttatgtacg gctccaaggc ctatgtgaaa catccagctg
     1381 acattcccga ttataagaaa ctgagcttcc ccgaggggtt taagtgggaa agagtgatga
     1441 acttcgagga cggaggcctg gtgactgtga cccaggacag ctccctgcag gatgggaccc
     1501 tgatctacaa ggtgaaaatg agagggacaa attttccccc tgatggacct gtgatgcaga
     1561 agaaaactat gggatgggag gcctccaccg aaaggctgta tccacgcgac ggggtgctga
     1621 aaggagaaat ccaccaggct ctgaagctga aagatggggg acattacctg gtggagttca
     1681 agacaatcta catggccaag aaacctgtgc agctgccagg ctactattac gtggacacaa
     1741 aactggatat cacttcacac aacgaggact acactattgt ggagcagtat gaacggagcg
     1801 aggggagaca ccatctgttc ctgggccatg ggactggaag taccggctca gggtctagtg
     1861 gaaccgcctc aagcgaggat aacaatatgg ctgtgatcaa agagttcatg aggtttaagg
     1921 tgcgcatgga gggcagcatg aatgggcacg aatttgagat tgaaggagag ggcgaaggga
     1981 ggccttacga gggcacacag actgccaagc tgaaagtgac caagggagga ccactgcctt
     2041 tcgcttggga tatcctgtct cctcagttta tgtacggaag taaggcctat gtcaagcatc
     2101 ccgctgacat tcctgattac aagaaactgt ctttcccaga gggctttaag tgggagagag
     2161 tgatgaattt tgaagatgga ggcctggtga ccgtgacaca ggactcctct ctgcaggatg
     2221 gcactctgat ctacaaagtc aaaatgcgcg gcaccaattt tccacccgat gggcccgtga
     2281 tgcagaagaa aacaatgggg tgggaggcca gcactgaacg gctgtatcct agagacggag
     2341 tgctgaaggg cgaaatccac caggccctga agctgaaaga cggcggccac tacctggtgg
     2401 agttcaaaac catctacatg gccaagaaac cagtgcagct gcccggctat tactatgtgg
     2461 acaccaagct ggatatcaca tcccacaatg aagactacac cattgtggaa cagtatgaga
     2521 ggtctgaagg acgccaccat ctgtttctgt acggcatgga tgagctgtat aagtaagaat
     2581 tcgatatcaa gcttatcgat aatcaacctc tggattacaa aatttgtgaa agattgactg
     2641 gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt
     2701 atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc
     2761 tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt
     2821 ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga
     2881 ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct
     2941 gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaaatcat
     3001 cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct
     3061 gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc
     3121 tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg
     3181 cctccccgca tcgataccgt cgacccgggc ggccgcttcg agcagacatg ataagataca
     3241 ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa
     3301 tttgtgatgc tattgcttta tttgtaacca ttataagctg caataaacaa gttaacaaca
     3361 acaattgcat tcattttatg tttcaggttc agggggagat gtgggaggtt ttttaaagca
     3421 agtaaaacct ctacaaatgt ggtaaaatcg ataaggatct tcctagagca tggctacgta
     3481 gataagtagc atggcgggtt aatcattaac tacaaggaac ccctagtgat ggagttggcc
     3541 actccctctc tgcgcgctcg ctcgctcact gaggccgggc gaccaaaggt cgcccgacgc
     3601 ccgggctttg cccgggcggc ctcagtgagc gagcgagcgc gcagctgcat taatgaatcg
     3661 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc attaatgaat cggccaacgc
     3721 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg
     3781 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
     3841 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
     3901 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
     3961 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     4021 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     4081 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
     4141 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     4201 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     4261 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     4321 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta
     4381 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
     4441 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     4501 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
     4561 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
     4621 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
     4681 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
     4741 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
     4801 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
     4861 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
     4921 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
     4981 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
     5041 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
     5101 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
     5161 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
     5221 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
     5281 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
     5341 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
     5401 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
     5461 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
     5521 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
     5581 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
     5641 caaatagggg ttccgcgcac atttccccga aaagtgccac ctaaattgta agcgttaata
     5701 ttttgttaaa attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg
     5761 aaatcggcaa aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc
     5821 cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa
     5881 ccgtctatca gggcgatggc ccactacgtg aaccatcacc ctaatcaagt tttttggggt
     5941 cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac
     6001 ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta
     6061 gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg
     6121 cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg caactgttgg gaagggcgat
     6181 cggtgcgggc ctcttcgcta ttacgcca
//
