LOCUS       Exported                6403 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Measles virus hemagglutinin expressing plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    pCG-Hc∆18
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6403)
  AUTHORS   Marino MP, Panigaj M, Ou W, Manirarora J, Wei CH, Reiser J
  TITLE     A scalable method to concentrate lentiviral vectors pseudotyped with
            measles virus glycoproteins.
  JOURNAL   Gene Ther. 2015 Mar;22(3):280-5. doi: 10.1038/gt.2014.125. Epub 2015
            Jan 22.
  PUBMED    25608718
REFERENCE   2  (bases 1 to 6403)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6403
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        17..320
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        321..524
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     475..495
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     521..545
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          645..1218
                     /label=-beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     1226..1245
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     promoter        1273..1291
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3384..3403)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    3449..3504
                     /label=-beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(3503..3522)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     rep_origin      3858..3993
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3912..3931)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     complement(4099..4116)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4270..4858)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4350..4369)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(5029..5889)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5652..5671
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5890..5994)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     6062..6080
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(6118..6140)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     6240..6259
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        6378..6396
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gaattcgagc tcgccccgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
       61 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      121 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      181 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      241 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      301 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      361 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      421 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      481 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc
      541 agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac cgggaccgat
      601 ccagcctccg ggggatcgat ccccccgatc ctgagaactt cagggtgagt ttggggaccc
      661 ttgattgttc tttctttttc gctattgtaa aattcatgtt atatggaggg ggcaaagttt
      721 tcagggtgtt gtttagaatg ggaagatgtc ccttgtatca ccatggaccc tcatgataat
      781 tttgtttctt tcactttcta ctctgttgac aaccattgtc tcctcttatt ttcttttcat
      841 tttctgtaac tttttcgtta aactttagct tgcatttgta acgaattttt aaattcactt
      901 ttgtttattt gtcagattgt aagtactttc tctaatcact ttttttttca aggcaatcag
      961 ggtatattat attgtacttc agcacagttt tagagaacaa ttgttataat taaatgataa
     1021 ggtagaatat ttctgcatat aaattctggc tggcgtggaa atattcttat tggtagaaac
     1081 aactacatcc tggtcatcat cctgcctttc tctttatggt tacaatgata tacactgttt
     1141 gagatgagga taaaatactc tgagtccaaa ccgggcccct ctgctaacca tgttcatgcc
     1201 ttcttctttt tcctacagct cctgggcaac gtgctggtta ttgtgctgtc tcatcatttt
     1261 ggcaaagaat tgtaatacga ctcactatag ggcgaattcg gatcccccgg tagttaatta
     1321 aaaattaggg tgcaagatca tcgataatgg gcagccggat cgtgatcaac cgggagcacc
     1381 tgatgatcga ccggccctac gtgctgctgg ccgtgctgtt cgtgatgttc ctgagcctga
     1441 tcggcttgct agccattgct ggaatccggc tgcacagagc cgccatctac accgccgaga
     1501 tccacaagag cctgagcacc aacctggacg tgaccaacag catcgagcat caggtcaagg
     1561 acgtgctgac ccccctgttt aagatcatcg gcgacgaagt gggcctgcgg accccccaga
     1621 gattcaccga cctggtcaag ttcatcagcg acaagatcaa gttcctgaac cccgaccggg
     1681 agtacgactt ccgggacctg acctggtgca tcaacccccc cgagcggatc aagctggact
     1741 acgaccagta ctgcgccgat gtggccgccg aggaactgat gaatgcattg gtgaactcaa
     1801 ctctactgga gaccagaaca accaatcagt tcctagctgt ctcaaaggga aactgctcag
     1861 ggcccactac aatcagaggt caattctcaa acatgtcgct gtccctgtta gacttgtatt
     1921 taggtcgagg ttacaatgtg tcatctatag tcactatgac atcccaggga atgtatgggg
     1981 gaacttacct agtggaaaag cctaatctga gcagcaaaag gtcagagttg tcacaactga
     2041 gcatgtaccg agtgtttgaa gtaggtgtta tcagaaatcc gggtttgggg gctccggtgt
     2101 tccatatgac aaactatctt gagcaaccag tcagtaatga tctcagcaac tgtatggtgg
     2161 ctttggggga gctcaaactc gcagcccttt gtcacgggga agattctatc acaattccct
     2221 atcagggatc agggaaaggt gtcagcttcc agctcgtcaa gctaggtgtc tggaaatccc
     2281 caaccgacat gcaatcctgg gtccccttat caacggatga tccagtgata gacaggcttt
     2341 acctctcatc tcacagaggt gttatcgctg acaaccaagc aaaatgggct gtcccgacaa
     2401 cacgaacaga tgacaagttg cgaatggaga catgcttcca acaggcgtgt aagggtaaaa
     2461 tccaagcact ctgcgagaat cccgagtggg caccattgaa ggataacagg attccttcat
     2521 acggggtctt gtctgttgat ctgagtctga cagttgagct taaaatcaaa attgcttcgg
     2581 gattcgggcc attgatcaca cacggttcag ggatggacct atacaaatcc aaccacaaca
     2641 atgtgtattg gctgactatc ccgccaatga agaacctagc cttaggtgta atcaacacat
     2701 tggagtggat accgagattc aaggttagtc cctacctctt cactgtccca attaaggaag
     2761 caggcggaga ctgccatgcc ccaacatacc tacctgcgga ggtggatggt gatgtcaaac
     2821 tcagttccaa tctggtgatt ctacctggtc aagatctcca atatgttttg gcaacctacg
     2881 atacttccag ggttgaacat gctgtggttt attacgttta cagcccaagc cgctcatttt
     2941 cttactttta tccttttagg ttgcctataa agggggtccc catcgaatta caagtggaat
     3001 gcttcacatg ggaccaaaaa ctctggtgcc gtcacttctg tgtgcttgcg gactcagaat
     3061 ctggtggaca tatcactcac tctgggatgg tgggcatggg agtcagctgc acagtcaccc
     3121 gggaagatgg aaccaatcgc agatagggct gctagtgaac caatcacatg atgtcaccca
     3181 gacatcaggc atacccacta gtgtgaaata gacatcagaa ttaagaaaaa cgtagggtcc
     3241 aagtggttcc ccgttatgga ctcgctatct gtcaaccaga tcttataccc tgaagttcac
     3301 ctagatagcc cgatagttac caataagata gtagccatcc tggagtatgc tcgactgttt
     3361 aaacctgcag gcatgcaagc tgatcttttt ccctctgcca aaaattatgg ggacatcatg
     3421 aagccccttg agcatctgac ttctggctaa taaaggaaat ttattttcat tgcaatagtg
     3481 tgttggaatt ttttgtgtct ctcactcgga aggacatatg ggagggcaaa tcatttaaaa
     3541 catcagaatg agtatttggt ttagagtttg gcaacatatg cccatatgct ggctgccatg
     3601 aacaaaggtt ggctataaag aggtcatcag tatatgaaac agccccctgc tgtccattcc
     3661 ttattccata gaaaagcctt gacttgaggt tagatttttt ttatattttg ttttgtgtta
     3721 tttttttctt taacatccct aaaattttcc ttacatgttt tactagccag atttttcctc
     3781 ctctcctgac tactcccagt catagctgtc cctcttctct tatggagatc cctcgaggag
     3841 ctttttgcaa aagcctaggc ctccaaaaaa gcctcttcac tacttctgga atagctcaga
     3901 ggccgaggcg gcctcggcct ctgcataaat aaaaaaaatt agtcagccat ggggcggaga
     3961 atgggcggaa ctgggcggag ttaggggcgg gatgggcgga gttaggggcg ggactatggt
     4021 tgctgactaa ttgctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     4081 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     4141 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     4201 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     4261 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     4321 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     4381 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     4441 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     4501 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     4561 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     4621 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     4681 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
     4741 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     4801 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     4861 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     4921 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     4981 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     5041 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     5101 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     5161 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     5221 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     5281 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     5341 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     5401 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     5461 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     5521 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     5581 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
     5641 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
     5701 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
     5761 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
     5821 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
     5881 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
     5941 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
     6001 tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa
     6061 aaataggcgt atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct
     6121 ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag
     6181 acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc ttaactatgc
     6241 ggcatcagag cagattgtac tgagagtgca ccattcgacg ctctccctta tgcgactcct
     6301 gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg
     6361 gtctggctta tcgaaattaa tacgactcac tatagggaga ccg
//
