LOCUS       Exported                6763 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Plasmid encodes blinded measles virus H glycoprotein displaying 
            IL-13.
ACCESSION   .
VERSION     .
KEYWORDS    pCG-Hc∆18-AA-IL-13
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6763)
  AUTHORS   Ou W, Marino MP, Suzuki A, Joshi B, Husain SR, Maisner A, Galanis E,
            Puri RK, Reiser J
  TITLE     Specific targeting of human interleukin (IL)-13 receptor 
            alpha2-positive cells with lentiviral vectors displaying IL-13.
  JOURNAL   Hum Gene Ther Methods. 2012 Apr;23(2):137-47. Epub 2012 May 21.
  PUBMED    22612657
REFERENCE   2  (bases 1 to 6763)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6763
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        17..320
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        321..524
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     475..495
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     521..545
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          645..1218
                     /label=-beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     1226..1245
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     promoter        1273..1291
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             3138..3149
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             3528..3545
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(3744..3763)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    3809..3864
                     /label=-beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(3863..3882)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     rep_origin      4218..4353
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4272..4291)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     complement(4459..4476)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4630..5218)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4710..4729)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(5389..6249)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6012..6031
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6250..6354)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     6422..6440
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(6478..6500)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     6600..6619
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        6738..6756
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gaattcgagc tcgccccgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
       61 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      121 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      181 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      241 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      301 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      361 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      421 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      481 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc
      541 agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagacac cgggaccgat
      601 ccagcctccg ggggatcgat ccccccgatc ctgagaactt cagggtgagt ttggggaccc
      661 ttgattgttc tttctttttc gctattgtaa aattcatgtt atatggaggg ggcaaagttt
      721 tcagggtgtt gtttagaatg ggaagatgtc ccttgtatca ccatggaccc tcatgataat
      781 tttgtttctt tcactttcta ctctgttgac aaccattgtc tcctcttatt ttcttttcat
      841 tttctgtaac tttttcgtta aactttagct tgcatttgta acgaattttt aaattcactt
      901 ttgtttattt gtcagattgt aagtactttc tctaatcact ttttttttca aggcaatcag
      961 ggtatattat attgtacttc agcacagttt tagagaacaa ttgttataat taaatgataa
     1021 ggtagaatat ttctgcatat aaattctggc tggcgtggaa atattcttat tggtagaaac
     1081 aactacatcc tggtcatcat cctgcctttc tctttatggt tacaatgata tacactgttt
     1141 gagatgagga taaaatactc tgagtccaaa ccgggcccct ctgctaacca tgttcatgcc
     1201 ttcttctttt tcctacagct cctgggcaac gtgctggtta ttgtgctgtc tcatcatttt
     1261 ggcaaagaat tgtaatacga ctcactatag ggcgaattcg gatcccccgg tagttaatta
     1321 aaaattaggg tgcaagatca tcgataatgg gcagccggat cgtgatcaac cgggagcacc
     1381 tgatgatcga ccggccctac gtgctgctgg ccgtgctgtt cgtgatgttc ctgagcctga
     1441 tcggcttgct agccattgct ggaatccggc tgcacagagc cgccatctac accgccgaga
     1501 tccacaagag cctgagcacc aacctggacg tgaccaacag catcgagcat caggtcaagg
     1561 acgtgctgac ccccctgttt aagatcatcg gcgacgaagt gggcctgcgg accccccaga
     1621 gattcaccga cctggtcaag ttcatcagcg acaagatcaa gttcctgaac cccgaccggg
     1681 agtacgactt ccgggacctg acctggtgca tcaacccccc cgagcggatc aagctggact
     1741 acgaccagta ctgcgccgat gtggccgccg aggaactgat gaatgcattg gtgaactcaa
     1801 ctctactgga gaccagaaca accaatcagt tcctagctgt ctcaaaggga aactgctcag
     1861 ggcccactac aatcagaggt caattctcaa acatgtcgct gtccctgtta gacttgtatt
     1921 taggtcgagg ttacaatgtg tcatctatag tcactatgac atcccaggga atgtatgggg
     1981 gaacttacct agtggaaaag cctaatctga gcagcaaaag gtcagagttg tcacaactga
     2041 gcatgtaccg agtgtttgaa gtaggtgtta tcagaaatcc gggtttgggg gctccggtgt
     2101 tccatatgac aaactatctt gagcaaccag tcagtaatga tctcagcaac tgtatggtgg
     2161 ctttggggga gctcaaactc gcagcccttt gtcacgggga agattctatc acaattccct
     2221 atcagggatc agggaaaggt gtcagcttcc agctcgtcaa gctaggtgtc tggaaatccc
     2281 caaccgacat gcaatcctgg gtccccttat caacggatga tccagtgata gacaggcttt
     2341 acctctcatc tcacagaggt gttatcgctg acaaccaagc aaaatgggct gtcccgacaa
     2401 cacgaacaga tgacaagttg cgaatggaga catgcttcca acaggcgtgt aagggtaaaa
     2461 tccaagcact ctgcgagaat cccgagtggg caccattgaa ggataacagg attccttcat
     2521 acggggtctt gtctgttgat ctgagtctga cagttgagct taaaatcaaa attgcttcgg
     2581 gattcgggcc attgatcaca cacggttcag ggatggacct atacaaatcc aaccacaaca
     2641 atgtgtattg gctgactatc ccgccaatga agaacctagc cttaggtgta atcaacacat
     2701 tggagtggat accgagattc aaggttagtc ccgcgctctt caatgtccca attaaggaag
     2761 caggcggaga ctgccatgcc ccaacatacc tacctgcgga ggtggatggt gatgtcaaac
     2821 tcagttccaa tctggtgatt ctacctggtc aagatctcca atatgttttg gcaacctacg
     2881 atacttccgc ggttgaacat gctgtggttt attacgttta cagcccaagc cgctcatttt
     2941 cttactttta tccttttagg ttgcctataa agggggtccc catcgaatta caagtggaat
     3001 gcttcacatg ggaccaaaaa ctctggtgcc gtcacttctg tgtgcttgcg gactcagaat
     3061 ctggtggaca tatcactcac tctgggatgg tgggcatggg agtcagctgc acagtcaccc
     3121 gggaagatgg aaccaatatc gagggaaggg cggcccagcc ggccatgtcc ccaggccctg
     3181 tgcctccctc tacagccctc agggagctca ttgaggagct ggtcaacatc acccagaacc
     3241 agaaggctcc gctctgcaat ggcagcatgg tatggagcat caacctgaca gctggcatgt
     3301 actgtgcagc cctggaatcc ctgatcaacg tgtcaggctg cagtgccatc gagaagaccc
     3361 agaggatgct gagcggattc tgcccgcaca aggtctcagc tgggcagttt tccagcttgc
     3421 atgttcgaga caccaaaatc gaggtggccc agtttgtaaa agatctgctc ttacatttaa
     3481 agaaactttt tcgcgaggga cagttcaacg cggccgcaag aggatcgcat caccatcacc
     3541 atcactgata gtttgtacta gtgtgaaata gacatcagaa ttaagaaaaa cgtagggtcc
     3601 aagtggttcc ccgttatgga ctcgctatct gtcaaccaga tcttataccc tgaagttcac
     3661 ctagatagcc cgatagttac caataagata gtagccatcc tggagtatgc tcgactgttt
     3721 aaacctgcag gcatgcaagc tgatcttttt ccctctgcca aaaattatgg ggacatcatg
     3781 aagccccttg agcatctgac ttctggctaa taaaggaaat ttattttcat tgcaatagtg
     3841 tgttggaatt ttttgtgtct ctcactcgga aggacatatg ggagggcaaa tcatttaaaa
     3901 catcagaatg agtatttggt ttagagtttg gcaacatatg cccatatgct ggctgccatg
     3961 aacaaaggtt ggctataaag aggtcatcag tatatgaaac agccccctgc tgtccattcc
     4021 ttattccata gaaaagcctt gacttgaggt tagatttttt ttatattttg ttttgtgtta
     4081 tttttttctt taacatccct aaaattttcc ttacatgttt tactagccag atttttcctc
     4141 ctctcctgac tactcccagt catagctgtc cctcttctct tatggagatc cctcgaggag
     4201 ctttttgcaa aagcctaggc ctccaaaaaa gcctcttcac tacttctgga atagctcaga
     4261 ggccgaggcg gcctcggcct ctgcataaat aaaaaaaatt agtcagccat ggggcggaga
     4321 atgggcggaa ctgggcggag ttaggggcgg gatgggcgga gttaggggcg ggactatggt
     4381 tgctgactaa ttgctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     4441 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     4501 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     4561 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     4621 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     4681 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     4741 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     4801 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     4861 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     4921 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     4981 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     5041 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
     5101 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     5161 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     5221 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     5281 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     5341 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     5401 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     5461 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     5521 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     5581 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     5641 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     5701 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     5761 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     5821 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     5881 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     5941 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
     6001 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
     6061 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
     6121 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
     6181 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
     6241 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
     6301 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
     6361 tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa
     6421 aaataggcgt atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct
     6481 ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag
     6541 acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc ttaactatgc
     6601 ggcatcagag cagattgtac tgagagtgca ccattcgacg ctctccctta tgcgactcct
     6661 gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg
     6721 gtctggctta tcgaaattaa tacgactcac tatagggaga ccg
//
