LOCUS Exported 7299 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Mammalian expression of Myc tagged N90 beta-catenin. ACCESSION . VERSION . KEYWORDS pT3-EF1aH N90-beta-catenin SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 7299) AUTHORS Tward AD, Jones KD, Yant S, Cheung ST, Fan ST, Chen X, Kay MA, Wang R, Bishop JM TITLE Distinct pathways of genomic progression to benign and malignant tumors of the liver. JOURNAL Proc Natl Acad Sci U S A. 2007 Sep 11;104(37):14771-6. Epub 2007 Sep 4. PUBMED 17785413 REFERENCE 2 (bases 1 to 7299) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..7299 /organism="synthetic DNA construct" /mol_type="other DNA" promoter 1..1179 /label=EF-1-alpha promoter /note="strong constitutive promoter for human elongation factor EF-1-alpha" intron 232..1170 /label=EF-1-alpha intron A /note="intron upstream of the start codon of human EF-1-alpha" primer_bind 1127..1147 /label=EF1a-F /note="Human elongation factor-1a promoter, forward primer" protein_bind 1210..1234 /gene="mutant version of attB" /label=attB1 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" CDS 1265..1294 /codon_start=1 /product="Myc (human c-Myc proto-oncogene) epitope tag" /label=Myc /translation="EQKLISEEDL" protein_bind complement(3406..3430) /gene="mutant version of attB" /label=attB2 /bound_moiety="BP Clonase(TM)" /note="recombination site for the Gateway(R) BP reaction" primer_bind 3458..3477 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind complement(3512..3529) /label=BGH-rev /note="Bovine growth hormone terminator, reverse primer. Also called BGH reverse" polyA_signal 3518..3742 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" primer_bind complement(4160..4176) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(4160..4176) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(4173..4195) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 4184..4200 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(4208..4238) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 4253..4274 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(4391..4408) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(4562..5150) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(4642..4661) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(5321..6181) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 5944..5963 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(6182..6286) /gene="bla" /label=AmpR promoter primer_bind 6354..6372 /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" primer_bind complement(6410..6432) /label=pGEX 3' /note="pGEX vectors, reverse primer" primer_bind 6532..6551 /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" primer_bind 6745..6767 /label=M13/pUC Forward /note="In lacZ gene" primer_bind 6759..6776 /label=M13 Forward /note="In lacZ gene. Also called M13-F20 or M13 (-21) Forward" primer_bind 6760..6776 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" primer_bind complement(7158..7178) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" ORIGIN 1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg 61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt 121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca 181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc 241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt 301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg 361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg 421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct 481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg 541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc 601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga 661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct 721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca 781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg 841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg 901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat 961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg 1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa 1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca 1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gaattagctt ggtaccaata 1201 cggatatcaa caagtttgta caaaaaagca ggctttaaag gaaccaattc agtcgaccac 1261 catggagcag aagctgatct ccgaggagga cctgaaggat cctcgagctc agagggtacg 1321 agctgctatg ttccctgaga cattagatga gggcatgcag atcccatcta cacagtttga 1381 tgctgctcat cccactaatg tccagcgttt ggctgaacca tcacagatgc tgaaacatgc 1441 agttgtaaac ttgattaact atcaagatga tgcagaactt gccacacgtg caatccctga 1501 actgacaaaa ctgctaaatg acgaggacca ggtggtggtt aataaggctg cagttatggt 1561 ccatcagctt tctaaaaagg aagcttccag acacgctatc atgcgttctc ctcagatggt 1621 gtctgctatt gtacgtacca tgcagaatac aaatgatgta gaaacagctc gttgtaccgc 1681 tgggaccttg cataaccttt cccatcatcg tgagggctta ctggccatct ttaagtctgg 1741 aggcattcct gccctggtga aaatgcttgg ttcaccagtg gattctgtgt tgttttatgc 1801 cattacaact ctccacaacc ttttattaca tcaagaagga gctaaaatgg cagtgcgttt 1861 agctggtggg ctgcagaaaa tggttgcctt gctcaacaaa acaaatgtta aattcttggc 1921 tattacgaca gactgccttc aaattttagc ttatggcaac caagaaagca agctcatcat 1981 actggctagt ggtggacccc aagctttagt aaatataatg aggacctata cttacgaaaa 2041 actactgtgg accacaagca gagtgctgaa ggtgctatct gtctgctcta gtaataagcc 2101 ggctattgta gaagctggtg gaatgcaagc tttaggactt cacctgacag atccaagtca 2161 acgtcttgtt cagaactgtc tttggactct caggaatctt tcagatgctg caactaaaca 2221 ggaagggatg gaaggtctcc ttgggactct tgttcagctt ctgggttcag atgatataaa 2281 tgtggtcacc tgtgcagctg gaattctttc taacctcact tgcaataatt ataagaacaa 2341 gatgatggtc tgccaagtgg gtggtataga ggctcttgtg cgtactgtcc ttcgggctgg 2401 tgacagggaa gacatcactg agcctgccat ctgtgctctt cgtcatctga ccagccgaca 2461 ccaagaagca gagatggccc agaatgcagt tcgccttcac tatggactac cagttgtggt 2521 taagctctta cacccaccat cccactggcc tctgataaag gctactgttg gattgattcg 2581 aaatcttgcc ctttgtcccg caaatcatgc acctttgcgt gagcagggtg ccattccacg 2641 actagttcag ttgcttgttc gtgcacatca ggatacccag cgccgtacgt ccatgggtgg 2701 gacacagcag caatttgtgg agggggtccg catggaagaa atagttgaag gttgtaccgg 2761 agcccttcac atcctagctc gggatgttca caaccgaatt gttatcagag gactaaatac 2821 cattccattg tttgtgcagc tgctttattc tcccattgaa aacatccaaa gagtagctgc 2881 aggggtcctc tgtgaacttg ctcaggacaa ggaagctgca gaagctattg aagctgaggg 2941 agccacagct cctctgacag agttacttca ctctaggaat gaaggtgtgg cgacatatgc 3001 agctgctgtt ttgttccgaa tgtctgagga caagccacaa gattacaaga aacggctttc 3061 agttgagctg accagctctc tcttcagaac agagccaatg gcttggaatg agactgctga 3121 tcttggactt gatattggtg cccagggaga accccttgga tatcgccagg atgatcctag 3181 ctatcgttct tttcactctg gtggatatgg ccaggatgcc ttgggtatgg accccatgat 3241 ggaacatgag atgggtggcc accaccctgg tgctgactat ccagttgatg ggctgccaga 3301 tctggggcat gcccaggacc tcatggatgg gctgcctcca ggtgacagca atcagctggc 3361 ctggtttgat actgacctgt aagcggccgc actcgagata tctagaccca gctttcttgt 3421 acaaagtggt tgatatcgcg gccgctgcat tctagttgtg gtttgtccaa actcatcaat 3481 gtatcttatc atgtctggat ccgctgatca gcctcgactg tgccttctag ttgccagcca 3541 tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc 3601 ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg 3661 gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct 3721 ggggatgcgg tgggctctat ggcttctgag gcgggatccc cttgaaatac atccacaggt 3781 acacctccaa ttgactcaaa tgatgtcaat tagtctatca gaagcttcta aagccatgac 3841 atcattttct ggaattttcc aagctgttta aaggcacagt caacttagtg tatgtaaact 3901 tctgacccac tggaattgtg atacagtgaa ttataagtga aataatctgt ctgtaaacaa 3961 ttgttggaaa aatgacttgt gtcatgcaca aagtagatgt cctaactgac ttgccaaaac 4021 tattgtttgt taacaagaaa tttgtggagt agttgaaaaa cgagttttaa tgactccaac 4081 ttaagtgtat gtaaacttcc gacttcaact gtataggtct agagtcgacc tgcaggcatg 4141 caagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa 4201 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga 4261 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt 4321 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct 4381 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat 4441 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga 4501 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt 4561 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt 4621 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc 4681 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa 4741 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct 4801 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta 4861 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg 4921 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc 4981 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta 5041 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg 5101 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt 5161 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg 5221 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta 5281 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg 5341 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg 5401 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc 5461 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg 5521 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg 5581 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag 5641 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat 5701 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc 5761 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc 5821 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa 5881 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac 5941 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt 6001 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc 6061 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa 6121 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca 6181 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat 6241 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa 6301 aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc 6361 gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca 6421 tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc 6481 gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg gcttaactat gcggcatcag 6541 agcagattgt actgagagtg caccatatgc ggtgtgaaat accgcacaga tgcgtaagga 6601 gaaaataccg catcaggcgc cattcgccat tcaggctgcg caactgttgg gaagggcgat 6661 cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat 6721 taagttgggt aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgaat 6781 tcgagctcgg taccctacag ttgaagtcgg aagtttacat acacttaagt tggagtcatt 6841 aaaactcgtt tttcaactac tccacaaatt tcttgttaac aaacaatagt tttggcaagt 6901 cagttaggac atctactttg tgcatgacac aagtcatttt tccaacaatt gtttacagac 6961 agattatttc acttataatt cactgtatca caattccagt gggtcagaag tttacataca 7021 ctaagttgac tgtgccttta aacagcttgg aaaattccag aaaatgatgt catggcttta 7081 gaagcttctg atagactaat tgacatcatt tgagtcaatt ggaggtgtac ctgtggatgt 7141 atttcaagga attctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc 7201 agcaggcaga agtatgcaaa gcatgcatat cgatactagt ttaattaaga tcctctttgc 7261 agctaatgga ccttctaggt cttgaaagga gtgggaatt //