LOCUS Exported 9815 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Lentivirus to stably express fluorescent human protein in mammalian cells. ACCESSION . VERSION . KEYWORDS pHAGE2 Lnp-mCherry SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 9815) AUTHORS Wang S, Tukachinsky H, Romano FB, Rapoport TA TITLE Cooperation of the ER-shaping proteins atlastin, lunapark, and reticulons to generate a tubular membrane network. JOURNAL Elife. 2016 Sep 13;5. pii: e18605. doi: 10.7554/eLife.18605. PUBMED 27619977 REFERENCE 2 (bases 1 to 9815) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..9815 /organism="synthetic DNA construct" /mol_type="other DNA" CDS 1369..2076 /codon_start=1 /product="monomeric derivative of DsRed fluorescent protein (Shaner et al., 2004)" /label=mCherry /note="mammalian codon-optimized" /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA EGRHSTGGMDELYK" primer_bind complement(1514..1532) /label=mCherry-R /note="mCherry, reverse primer" primer_bind 1782..1801 /label=mCherry-F /note="mCherry, forward primer" primer_bind complement(2091..2110) /label=T7 /note="T7 promoter, forward primer" promoter complement(2092..2110) /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" polyA_signal 2115..2249 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind 2193..2212 /label=SV40pA-R /note="SV40 polyA, reverse primer" misc_feature 2341..2926 /label=IRES2 /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" primer_bind complement(2507..2524) /label=IRES reverse /note="IRES internal ribosome entry site, reverse primer. Also called pCDH-rev" primer_bind 2734..2753 /label=IRES-F /note="IRES internal ribosome entry site, forward primer" CDS 2927..3322 /codon_start=1 /gene="Aspergillus terreus BSD" /product="blasticidin S deaminase" /label=BSD /note="confers resistance to blasticidin" /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI KAIVKDSDGQPTAVGIRELLPSGYVWEG" CDS 3332..3385 /codon_start=1 /product="2A peptide from Thosea asigna virus capsid protein" /label=T2A /note="Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides." /translation="EGRGSLLTCGDVEENPGP" CDS 3389..3409 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" misc_feature 3435..4023 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(3488..3508) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(3906..3917) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" LTR 4171..4351 /label=5' LTR (truncated) /note="truncated 5' long terminal repeat (LTR) from HIV-1" primer_bind complement(4398..4416) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 4484..4588 /gene="bla" /label=AmpR promoter CDS 4589..5449 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(4807..4826) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 5620..6208 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 6109..6128 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 6362..6379 /label=L4440 /note="L4440 vector, forward primer" primer_bind 6471..6490 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" protein_bind 6619..6640 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 6655..6685 /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 6693..6709 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." primer_bind 6698..6720 /label=M13/pUC Reverse /note="In lacZ gene" primer_bind 6717..6733 /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind 6717..6733 /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" LTR 6994..7627 /label=3' LTR /note="3' long terminal repeat (LTR) from HIV-1" misc_feature 7674..7799 /label=HIV-1 Psi /note="packaging signal of human immunodeficiency virus type 1" misc_feature 8296..8529 /label=RRE /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." CDS 8714..8758 /codon_start=1 /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /label=gp41 peptide /note="recognized by the 2H10 single-chain llama nanobody" /translation="KNEQELLELDKWASL" misc_feature 9054..9171 /label=cPPT/CTS /note="central polypurine tract and central termination sequence of HIV-1" enhancer 9223..9526 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 9527..9730 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 9681..9701 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" primer_bind 9727..9751 /label=LNCX /note="Human CMV promoter, forward primer" ORIGIN 1 atgggtggat tattttctcg atggaggaca aaaccttcaa ctgtagaagt tctagaaagt 61 atagataagg aaattcaagc attggaagaa tttagggaaa aaaatcagag attacaaaaa 121 ttatgggttg gaagattaat tctgtattcc tcagttctct atctgtttac atgcttaatt 181 gtatatttgt ggtatcttcc tgatgaattt acagcaagac ttgccatgac actcccattt 241 tttgcttttc cattgatcat ctggagcata agaacagtaa ttattttctt cttttccaag 301 agaacagaaa gaaataatga agcattggat gatttaaaat cccagaggaa aaaaatactt 361 gaagaagtca tggaaaaaga aacttacaag acggctaaat taattcttga aaggtttgat 421 ccggactcaa agaaagcaaa ggagtgtgag ccgccatctg ctggagcagc tgtaactgca 481 agacctggac aagagattcg tcagcgaact gcagctcaaa gaaacctttc tccaacacca 541 gcaagcccta accagggccc tcctccacaa gttccagtat ctcctggacc accaaaggac 601 agttctgccc ctggtggacc cccagaaagg actgttactc cagccctatc atcaaatgtg 661 ttaccaagac atcttggatc ccctgctact tcagtgcctg gaatgggtct tcatcctcca 721 ggtccacctt tagcaagacc tattctcccc cgagaacgag gtgctttgga tagaattgtt 781 gaatatttgg ttggtgatgg tccacaaaac aggtatgcac ttatatgtca gcagtgtttt 841 tctcataatg gcatggcttt gaaggaagaa tttgaataca ttgcttttcg atgtgcctac 901 tgttttttct tgaaccctgc aagaaaaacc agacctcagg ctccaagact tcctgagttt 961 agttttgaga agaggcaggt ggtggaaggt tcaagttcag ttggtccctt gccatcagga 1021 agtgtgcttt catcagacaa ccagtttaat gaagaatctt tagaacacga tgttcttgat 1081 gataatacag agcagacaga tgacaaaata ccagctacag aacagacaaa ccaagtgatt 1141 gaaaaagcat ctgactcaga ggaaccagag gagaaacaag agactgagaa tgaggaagcc 1201 tcagtgattg aaaccaactc cacagttcct ggagctgatt ctattcctga tcctgaacta 1261 agtggagaat ctttgacggc agaggctagt ggtggaggct ctgggggagg tacaggaggt 1321 ggctcaggtg ggggcactgg tggaggcagt ggaggtggca gatccaccat ggtgagcaag 1381 ggcgaggagg ataacatggc catcatcaag gagttcatgc gcttcaaggt gcacatggag 1441 ggctccgtga acggccacga gttcgagatc gagggcgagg gcgagggccg cccctacgag 1501 ggcacccaga ccgccaagct gaaggtgacc aagggtggcc ccctgccctt cgcctgggac 1561 atcctgtccc ctcagttcat gtacggctcc aaggcctacg tgaagcaccc cgccgacatc 1621 cccgactact tgaagctgtc cttccccgag ggcttcaagt gggagcgcgt gatgaacttc 1681 gaggacggcg gcgtggtgac cgtgacccag gactcctccc tgcaggacgg cgagttcatc 1741 tacaaggtga agctgcgcgg caccaacttc ccctccgacg gccccgtaat gcagaagaag 1801 accatgggct gggaggcctc ctccgagcgg atgtaccccg aggacggcgc cctgaagggc 1861 gagatcaagc agaggctgaa gctgaaggac ggcggccact acgacgctga ggtcaagacc 1921 acctacaagg ccaagaagcc cgtgcagctg cccggcgcct acaacgtcaa catcaagttg 1981 gacatcacct cccacaacga ggactacacc atcgtggaac agtacgaacg cgccgagggc 2041 cgccactcca ccggcggcat ggacgagctg tacaagtcta gattctgcag ccctatagtg 2101 agtcgtatta cgtagatcca gacatgataa gatacattga tgagtttgga caaaccacaa 2161 ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt gctttatttg 2221 taaccattat aagctgcaat aaacaagtta acaacaacaa ttgcattcat tttatgtttc 2281 aggttcaggg ggaggtgtgg gaggtttttt aattcgcggc cgctaagtaa gtaagatccg 2341 cccctctccc tccccccccc taacgttact ggccgaagcc gcttggaata aggccggtgt 2401 gcgtttgtct atatgttatt ttccaccata ttgccgtctt ttggcaatgt gagggcccgg 2461 aaacctggcc ctgtcttctt gacgagcatt cctaggggtc tttcccctct cgccaaagga 2521 atgcaaggtc tgttgaatgt cgtgaaggaa gcagttcctc tggaagcttc ttgaagacaa 2581 acaacgtctg tagcgaccct ttgcaggcag cggaaccccc cacctggcga caggtgcctc 2641 tgcggccaaa agccacgtgt ataagataca cctgcaaagg cggcacaacc ccagtgccac 2701 gttgtgagtt ggatagttgt ggaaagagtc aaatggctct cctcaagcgt attcaacaag 2761 gggctgaagg atgcccagaa ggtaccccat tgtatgggat ctgatctggg gcctcggtac 2821 acatgcttta catgtgttta gtcgaggtta aaaaaacgtc taggcccccc gaaccacggg 2881 gacgtggttt tcctttgaaa aacacgatga taatatggcc acaaccatgg ccaagccttt 2941 gtctcaagaa gaatccaccc tcattgaaag agcaacggct acaatcaaca gcatccccat 3001 ctctgaagac tacagcgtcg ccagcgcagc tctctctagc gacggccgca tcttcactgg 3061 tgtcaatgta tatcatttta ctgggggacc ttgtgcagaa ctcgtggtgc tgggcactgc 3121 tgctgctgcg gcagctggca acctgacttg tatcgtcgcg atcggaaatg agaacagggg 3181 catcttgagc ccctgcggac ggtgccgaca ggtgcttctc gatctgcatc ctgggatcaa 3241 agccatagtg aaggacagtg atggacagcc gacggcagtt gggattcgtg aattgctgcc 3301 ctctggttat gtgtgggagg gcggtagtgg cgagggcaga ggaagtcttc taacatgcgg 3361 tgacgtggag gagaatcccg gccctgctcc aaagaagaag cgtaaggtaa atcgatcgct 3421 agtcgagaga tcctaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc 3481 ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg 3541 ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc 3601 tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg 3661 acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg 3721 ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga 3781 caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct 3841 ttccttggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg 3901 tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc 3961 ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc 4021 cgcctggtac ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta 4081 aaagaaaagg ggggactgga agggctaatt cactcccaac gaagacaaga tcacctgcag 4141 gacaggcgcg ccctgctttt tgcttgtact gggtctctct ggttagacca gatctgagcc 4201 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga 4261 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga 4321 cccttttagt cagtgtggaa aatctctagc acccgggcga ttaaggaaag ggctagatca 4381 ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat 4441 aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg 4501 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat 4561 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat 4621 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt 4681 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag 4741 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa 4801 agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg 4861 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct 4921 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac 4981 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca 5041 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat 5101 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact 5161 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc 5221 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga 5281 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg 5341 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg 5401 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca 5461 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta 5521 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca 5581 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg 5641 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga 5701 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa 5761 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc 5821 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg 5881 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac 5941 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct 6001 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc 6061 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg 6121 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg 6181 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct 6241 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga 6301 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg 6361 cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc 6421 gcgttggccg attcattaat gcagcaagct catggctgac taattttttt tatttatgca 6481 gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga 6541 ggcctaggct tttgcaaaaa gctccccgtg gcacgacagg tttcccgact ggaaagcggg 6601 cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca 6661 ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg 6721 aaacagctat gacatgatta cgaatttcac aaataaagca tttttttcac tgcattctag 6781 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggatcaact ggataactca 6841 agctaaccaa aatcatccca aacttcccac cccataccct attaccactg ccaattacct 6901 gtggtttcat ttactctaaa cctgtgattc ctctgaatta ttttcatttt aaagaaattg 6961 tatttgttaa atatgtacta caaacttagt agttggaagg gctaattcac tcccaaagaa 7021 gacaagatat ccttgatctg tggatctacc acacacaagg ctacttccct gattagcaga 7081 actacacacc agggccaggg gtcagatatc cactgacctt tggatggtgc tacaagctag 7141 taccagttga gccagataag gtagaagagg ccaataaagg agagaacacc agcttgttac 7201 accctgtgag cctgcatggg atggatgacc cggagagaga agtgttagag tggaggtttg 7261 acagccgcct agcatttcat cacgtggccc gagagctgca tccggagtac ttcaagaact 7321 gctgatatcg agcttgctac aagggacttt ccgctgggga ctttccaggg aggcgtggcc 7381 tgggcgggac tggggagtgg cgagccctca gatcctgcat ataagcagct gctttttgcc 7441 tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg 7501 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt 7561 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc 7621 tctagcagtg gcgcccgaac agggacttga aagcgaaagg gaaaccagag gagctctctc 7681 gacgcaggac tcggcttgct gaagcgcgca cggcaagagg cgaggggcgg cgactgcaga 7741 gtacgccaaa aattttgact agcggaggct agaaggagag agatgggtgc gagagcgtca 7801 gtattaagcg ggggagaatt agatcgcgat gggaaaaaat tcggttaagg ccagggggaa 7861 agaaaaaata taaattaaaa catatagtat gggcaagcag ggagctagaa cgattcgcag 7921 ttaatcctgg cctgttagaa acatcagaag gctgtagaca aatactggga cagctacaac 7981 catcccttca gacaggatca gaagaactta gatcattata taatacagta gcaaccctct 8041 attgtgtgca tcaaaggata gagataaaag acaccaagga agctttagac aagatagagg 8101 aagagcaaaa caaaagtaag accaccgcac agcaagcggc cggccgctga tcttcagacc 8161 tggaggagga gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa 8221 aattgaacca ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa 8281 aagagcagtg ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat 8341 gggcgcagcg tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca 8401 gcagcagaac aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt 8461 ctggggcatc aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca 8521 acagctcctg gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg 8581 gaatgctagt tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga 8641 gtgggacaga gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca 8701 aaaccagcaa gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg 8761 gaattggttt aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg 8821 aggcttggta ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca 8881 gggatattca ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccgacaggcc 8941 cgaaggaata gaagaagaag gtggagagag agacagagac agatccattc gattagtgaa 9001 cggatctcga cggtatcgcc gaattctcaa caataggcag tattcatcca caattttaaa 9061 agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca 9121 gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat 9181 tactcggaca gcagagatcc agtttggact agtggagttc cgcgttacat aacttacgca 9241 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgaccaa 9301 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg 9361 caaaactgcc cacttggcag tacatcaagt gtatcatttg ccaacaacgc cccctattga 9421 cgtcaatgac gcaaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt 9481 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg 9541 gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc 9601 cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg 9661 taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat 9721 aagcagagct cgtttagtga accgtcagat cgcctggaga cgccatccac gctgttttga 9781 cctccattca agacaccggc ggccttaatt aaacc //